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The map label for this gene is opuAB [H]

Identifier: 86361123

GI number: 86361123

Start: 445449

End: 446297

Strand: Reverse

Name: opuAB [H]

Synonym: RHE_PF00393

Alternate gene names: 86361123

Gene position: 446297-445449 (Counterclockwise)

Preceding gene: 86361124

Following gene: 86361122

Centisome position: 69.46

GC content: 61.6

Gene sequence:

>849_bases
ATGGATACTTCAGTCTTAACCGATCTGTTCGACGAATGGACGGACTCCGCGCTCGAATGGGTGAGCGACAACGGCGAGTT
CCTCTTCGACTATATCAGGCAGGTGCTCGAGGGCCTCTATGACGGGATCCTCTGGCTCCTTGAGCTTCCGCCGTTCTACG
TGATCGCGATCATCGTGGCGCTGATCGGCTGGCGGCTGGTCAATGTCTGGTTCGCGGCGCTCAGCGGCGTCGCGCTGGCG
CTCTGCTTTTCGATGGGACTCTGGCCGGAGACGATGAGCACCCTGGCGCTGGTCCTGACAGCCACGGTGATCGCCTTGGC
GATCGGCATCCCGATCGGCATTGCGGCGGGCTTCTTCACCGCTCTTGACCGCTTCATGGAGCCGGGTCTCGATCTCATCC
AGACGCTTCCGCCATACATCTACCTGCTGCCGGCGATCGCCCTTCTCGGCTACGGACCGGCGACGGCGTTGATCGCGACC
GTGATCGTCGCCGTGCCGCCGGCGGTCCGCCTGACCTCGCTCGGTATCCGCATGACCCCCAAGGAGTTCATCGAACTTGG
CGAGGCGCTCGGGATGACTCCGGGAAAGATGTTCTTCAAGATCCGCCTTCCCTTTGCCCTGCCCAGCATGATGGCGGGCA
TTAACCAAAGCCTGATGATGGCCTTCGGCATGGTCGTCATCGCCGGCATCGTCGGTTCGGGCGGGCTCGGAGAGACGATC
TACGGCGCGATCAGGACGCTCGATATCGCGACTTCTATCAATGCGGCGATCGCCATCGTGGTATTGACCATGGTGATTGA
CCGAATAACGCAGAGCGCCGCTCGCTTGGGAACGGGGAGGAAGTCATGA

Upstream 100 bases:

>100_bases
GTCGTCGGCGTCGTCACGCCGCGCAGCCTGCTGATGGGCGTCAAGGGAACTTCCGCCCACGATCTAACGGCGGCGTGATC
CCCAACCGGAGCTGATCGAC

Downstream 100 bases:

>100_bases
ATCTTTCGGACTTGCAGTTTTCGCCCGGCGCTTTCCTGGCCCCATCAGTCGATTGGCTCAACACCAACCTTCATCCGCTG
TTTGCGGCCATCAGCTATGT

Product: glycine betaine/L-proline ABC transporter, permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MDTSVLTDLFDEWTDSALEWVSDNGEFLFDYIRQVLEGLYDGILWLLELPPFYVIAIIVALIGWRLVNVWFAALSGVALA
LCFSMGLWPETMSTLALVLTATVIALAIGIPIGIAAGFFTALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIAT
VIVAVPPAVRLTSLGIRMTPKEFIELGEALGMTPGKMFFKIRLPFALPSMMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YGAIRTLDIATSINAAIAIVVLTMVIDRITQSAARLGTGRKS

Sequences:

>Translated_282_residues
MDTSVLTDLFDEWTDSALEWVSDNGEFLFDYIRQVLEGLYDGILWLLELPPFYVIAIIVALIGWRLVNVWFAALSGVALA
LCFSMGLWPETMSTLALVLTATVIALAIGIPIGIAAGFFTALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIAT
VIVAVPPAVRLTSLGIRMTPKEFIELGEALGMTPGKMFFKIRLPFALPSMMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YGAIRTLDIATSINAAIAIVVLTMVIDRITQSAARLGTGRKS
>Mature_282_residues
MDTSVLTDLFDEWTDSALEWVSDNGEFLFDYIRQVLEGLYDGILWLLELPPFYVIAIIVALIGWRLVNVWFAALSGVALA
LCFSMGLWPETMSTLALVLTATVIALAIGIPIGIAAGFFTALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIAT
VIVAVPPAVRLTSLGIRMTPKEFIELGEALGMTPGKMFFKIRLPFALPSMMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YGAIRTLDIATSINAAIAIVVLTMVIDRITQSAARLGTGRKS

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=273, Percent_Identity=36.2637362637363, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=30.3664921465969, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788449, Length=237, Percent_Identity=29.535864978903, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30220; Mature: 30220

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSVLTDLFDEWTDSALEWVSDNGEFLFDYIRQVLEGLYDGILWLLELPPFYVIAIIVA
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LIGWRLVNVWFAALSGVALALCFSMGLWPETMSTLALVLTATVIALAIGIPIGIAAGFFT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIATVIVAVPPAVRLTSLGIRMTP
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCH
KEFIELGEALGMTPGKMFFKIRLPFALPSMMAGINQSLMMAFGMVVIAGIVGSGGLGETI
HHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
YGAIRTLDIATSINAAIAIVVLTMVIDRITQSAARLGTGRKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDTSVLTDLFDEWTDSALEWVSDNGEFLFDYIRQVLEGLYDGILWLLELPPFYVIAIIVA
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LIGWRLVNVWFAALSGVALALCFSMGLWPETMSTLALVLTATVIALAIGIPIGIAAGFFT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIATVIVAVPPAVRLTSLGIRMTP
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCH
KEFIELGEALGMTPGKMFFKIRLPFALPSMMAGINQSLMMAFGMVVIAGIVGSGGLGETI
HHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
YGAIRTLDIATSINAAIAIVVLTMVIDRITQSAARLGTGRKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]