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The map label for this gene is opuAB [H]

Identifier: 86361122

GI number: 86361122

Start: 444601

End: 445452

Strand: Reverse

Name: opuAB [H]

Synonym: RHE_PF00392

Alternate gene names: 86361122

Gene position: 445452-444601 (Counterclockwise)

Preceding gene: 86361123

Following gene: 86361121

Centisome position: 69.33

GC content: 64.79

Gene sequence:

>852_bases
ATGAATCTTTCGGACTTGCAGTTTTCGCCCGGCGCTTTCCTGGCCCCATCAGTCGATTGGCTCAACACCAACCTTCATCC
GCTGTTTGCGGCCATCAGCTATGTGGTCGAAACGGTGCTGTCCGCCATCGAGGCCGCACTTCTCTTCGTGCCGCCTTATG
CGCTGATCGCGATCGTCGTCGTGCTGGCATTCTTTGCCGTCGGCCTGCGCGCCGCCATCCTCGCCGGCCTCTGCCTCGGC
TTCTGCCTGCTTGTCGGCCTGTGGACGGCGTCGATGCAGACGCTTGCTCTGGTCACCGTCGCCGTCGTGATCTCCGTTCT
CATCGCCTTTCCGATCGGCGTGCTCTGCTCGCGTTACAAGGCATTGGAGGCCGCAGTTCGCCCGGTTCTCGACGTGATGC
AGACCGTGCCGCCATGGGTCTATCTCATTCCGGCGGTGATGATCTTCAGCCTCGGACGCGTGCCGGCGATCATCGCCACC
ATCGTCTATGGCATTCCGCCGATGCTGCGTCTGACGACACTTGCCTTCAACCAGGTGCCGAAGGTGTTCATCGAGCTCGG
CAGCGCCATCGGGGCCCCGCCACGCTCGATCCTGTGGAAGATCGAGATGCCGCTTGCCAAGCAGACGCTGCTGGTCGGCC
TCAACCAGTGCATCCTGCTGTCGCTTGCGATGGTCGTGCTCGCCGGCCTCGTCGGTGCGGGCGGGCTCGGCGCGGAAGTG
ACGCGCGGCCTGACGCGCATGGAAATGGGGCTCGGCCTCAGAGCGGGTCTGGCGATCGTTGCCGTCGCCCTGCTGCTCGA
CCGGCTGACCCGCGGTCTGTTCGACCGCGGCCGGCTGGCGAAGGCGCGGTGA

Upstream 100 bases:

>100_bases
TCGCGACTTCTATCAATGCGGCGATCGCCATCGTGGTATTGACCATGGTGATTGACCGAATAACGCAGAGCGCCGCTCGC
TTGGGAACGGGGAGGAAGTC

Downstream 100 bases:

>100_bases
GCCGGGATATGAAAAGATGAGAAACAGCTTCTTCTGCATCGATGCCCATACCTGCGGCAACCCGGTCCGTCTCGTCGCCG
GAGGCGGCCCGCTGCTTCCA

Product: glycine betaine/L-proline ABC transporter, permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNLSDLQFSPGAFLAPSVDWLNTNLHPLFAAISYVVETVLSAIEAALLFVPPYALIAIVVVLAFFAVGLRAAILAGLCLG
FCLLVGLWTASMQTLALVTVAVVISVLIAFPIGVLCSRYKALEAAVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPKVFIELGSAIGAPPRSILWKIEMPLAKQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLTRGLFDRGRLAKAR

Sequences:

>Translated_283_residues
MNLSDLQFSPGAFLAPSVDWLNTNLHPLFAAISYVVETVLSAIEAALLFVPPYALIAIVVVLAFFAVGLRAAILAGLCLG
FCLLVGLWTASMQTLALVTVAVVISVLIAFPIGVLCSRYKALEAAVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPKVFIELGSAIGAPPRSILWKIEMPLAKQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLTRGLFDRGRLAKAR
>Mature_283_residues
MNLSDLQFSPGAFLAPSVDWLNTNLHPLFAAISYVVETVLSAIEAALLFVPPYALIAIVVVLAFFAVGLRAAILAGLCLG
FCLLVGLWTASMQTLALVTVAVVISVLIAFPIGVLCSRYKALEAAVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPKVFIELGSAIGAPPRSILWKIEMPLAKQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLTRGLFDRGRLAKAR

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=272, Percent_Identity=37.1323529411765, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1788451, Length=202, Percent_Identity=29.7029702970297, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788449, Length=184, Percent_Identity=28.804347826087, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29994; Mature: 29994

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]