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The map label for this gene is gcvA [H]

Identifier: 86361117

GI number: 86361117

Start: 439096

End: 439983

Strand: Reverse

Name: gcvA [H]

Synonym: RHE_PF00387

Alternate gene names: 86361117

Gene position: 439983-439096 (Counterclockwise)

Preceding gene: 86361120

Following gene: 86361114

Centisome position: 68.48

GC content: 64.53

Gene sequence:

>888_bases
ATGCAATTTCGAAGACGGCTTCCATCGCTGACGGCGCTGGTGACGCTGGAAGCGGTGCTGCGCAGGAAAAGTTTCACCAC
GGCGGCGAGCGAACTCGGCGTCACCCAGGCGGCCGTCAGCCGGCAGATCGCCGCTTTGGAGGAGGAGCTCGGCCAGCCGC
TCTTCCTGCGTAAACACCGGGCGATCAAGCCAACGGCGGCCTGCGTCAGCCTCGGCGCGACGCTGGCGAAGAGCTTTGCC
GATATCGCCGAGAGCGTGGAGGCGATCCAGTCGCGCAGCCAGGATGTGGTGACGATCGGCGCAACCGTCGCCTTCTCTTC
CTTCTGGCTGCTGCCGCGGCTCGCCGAATTCCGCCGGGCCAATCCCGGCATTCTGGTGCGGGTGATATCCCAGGATAACC
CGATCACTCTTGATGACGGGGAGATCGACGTGGCGATCCGATACGGCCTGCCGCCCTTCAGCGATGGAACGGTCATTGCC
TCGCGCGGCGACGTCGTCTGCCCTGTCTGCTCTCCCGACCACCTCAGGCGCCGGGGAAATGGCCCTCTCAGTTCCACCGA
CGAATTCATCGAAACGGAGGTGATCGATCGTTCCTGGTATGGCTGGGGGCAATGGGTGTCGCTGACCGGCGGCAATATCG
AGGTCAAACCGTCGCTTCGCTTCAACCATTACACCGAAAGCATCGCGGCGGCGCGCGCGGGACAGGGGATTGCCTTGGGA
TGGCGCATGCTGATCGGCACGTTCCTGGAGGACGGAACGCTGGTGTCGGCTTCAGGAATCGAGCTTGCCGCCGAAGGCCG
CTACAACGTGATCGTGCCGATCAAGGCCAAGCGCAGCCATGCGGGCGATCTCGCCGCCGCCTGGCTGACAGCCTCGCTGC
ACGGCTGA

Upstream 100 bases:

>100_bases
ATGTGAGAGTTTCCCCAGATTGCTCTTGCAGCGCCGGGCTTTCAACGGCATAAAAAATTAGCCTCGCATAAGTTTTTATC
ATGCGAATAGGAGTCTCGTG

Downstream 100 bases:

>100_bases
TCAGAGGCCGAGGCCGGGTGTCGCCGTTCGATGAACGCCCTTCGGAACGAAAGCGAATTGATCCGCCAGCCCGAGGAAAT
CAGCCGCCAGGTGCACGACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHRAIKPTAACVSLGATLAKSFA
DIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRANPGILVRVISQDNPITLDDGEIDVAIRYGLPPFSDGTVIA
SRGDVVCPVCSPDHLRRRGNGPLSSTDEFIETEVIDRSWYGWGQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
WRMLIGTFLEDGTLVSASGIELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG

Sequences:

>Translated_295_residues
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHRAIKPTAACVSLGATLAKSFA
DIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRANPGILVRVISQDNPITLDDGEIDVAIRYGLPPFSDGTVIA
SRGDVVCPVCSPDHLRRRGNGPLSSTDEFIETEVIDRSWYGWGQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
WRMLIGTFLEDGTLVSASGIELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG
>Mature_295_residues
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHRAIKPTAACVSLGATLAKSFA
DIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRANPGILVRVISQDNPITLDDGEIDVAIRYGLPPFSDGTVIA
SRGDVVCPVCSPDHLRRRGNGPLSSTDEFIETEVIDRSWYGWGQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
WRMLIGTFLEDGTLVSASGIELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=272, Percent_Identity=32.7205882352941, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788706, Length=273, Percent_Identity=27.8388278388278, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=28.125, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=26.3888888888889, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787589, Length=243, Percent_Identity=27.5720164609054, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31866; Mature: 31866

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50931 HTH_LYSR ; PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHR
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEHHCC
AIKPTAACVSLGATLAKSFADIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
NPGILVRVISQDNPITLDDGEIDVAIRYGLPPFSDGTVIASRGDVVCPVCSPDHLRRRGN
CCCEEEEEECCCCCEEEECCCEEEEEEECCCCCCCCEEEECCCCEEEECCCHHHHHHCCC
GPLSSTDEFIETEVIDRSWYGWGQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCHHHH
WRMLIGTFLEDGTLVSASGIELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG
HHHHHHHHHCCCEEEEECCCEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHR
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEHHCC
AIKPTAACVSLGATLAKSFADIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
NPGILVRVISQDNPITLDDGEIDVAIRYGLPPFSDGTVIASRGDVVCPVCSPDHLRRRGN
CCCEEEEEECCCCCEEEECCCEEEEEEECCCCCCCCEEEECCCCEEEECCCHHHHHHCCC
GPLSSTDEFIETEVIDRSWYGWGQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCHHHH
WRMLIGTFLEDGTLVSASGIELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG
HHHHHHHHHCCCEEEEECCCEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]