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The map label for this gene is ygbJ [C]

Identifier: 86361075

GI number: 86361075

Start: 386846

End: 387790

Strand: Direct

Name: ygbJ [C]

Synonym: RHE_PF00345

Alternate gene names: 86361075

Gene position: 386846-387790 (Clockwise)

Preceding gene: 86361070

Following gene: 86361076

Centisome position: 60.21

GC content: 65.82

Gene sequence:

>945_bases
ATGACACCGCTCGCTGAAAACTCAGGCTCCGCCATCGTCGCGGCCGTCATCGGCCTCGGTTCCATGGGAATGGGAATGGC
CAGCTCAATGAAGCGCGCAGGTCTGGACGTCGTTGGATATGACATAACGCCGGCCGCGGTGGACCGCTTCGTCGCCGAAG
GCGGACGTGGCGCGGCATCCCCTGCCGACGCCGCCAAGGGGGCCGACATCATCGTCTCCGTGGTGGTCAACGGCGCGCAG
ACCGAAGCCGTGCTGTTCGGATCCAAGGGCGTTGCTGCTCCGATGAAGCGCGGCGCGGTCTTCATCTCGTCGGCGACGAT
GGACCCCGCCATCGCGCGCGATCTGGCGGCGAGGGCGCAGGCTCTCGGCCTGCACTATCTCGACGCGCCGATTTCAGGCG
GCGCGGCCAGGGCGGCGAAAGGTGAGCTGACGATCATGGCCTCCGGCTCCAGGCAGGCTTTCGATAGGGCGCGCCCGGCC
CTCGACGCCATGGCCGGCAAGGTTTACGAGCTCGGCGAGGCGGCGGGAACCGGCGCCGCCTTCAAGATGATCAACCAGCT
TCTCGCCGGCGTACATATCGCGGCCGCATGCGAGGCGATAACTTTTGCCGCCAAGCAGGGCCTCGACCTCGACAAGGTCT
ATGAAGTCATCACCGCCTCGGCGGGCAATTCCTGGATGTTTGAAAATCGTGTCCCGCACGTGCTGGCCGGGGACTATACG
CCGGCCAGCAGCATTGAGATTTTCGTCAAGGATCTCGGCATCGTACTGGATATGGCCCGTTCGCAGCGTTATCCGGCACC
GCTCGCGGCAGCGGCCCTGCAAATGTACCTGGCGGCCGCCGGCGCCGGCATGGGCGGCGACGATGATTCCTCGCTCGCAC
GCCTCTACGCCCAGCTTTCCGGCGCGCAATTGCCCGGTTTCGCCAAAGAGCCGCAAGGCCGGTAA

Upstream 100 bases:

>100_bases
TTTTTGGCAGCGCTGCCATTTGACTGTTGGCGAGGCCGCTGGAATGATCTAGAAAGAATGGCAGCGCTGCCAAAATGGCT
CGTGATCGGAGGACGTTCCC

Downstream 100 bases:

>100_bases
GGAGGGTCGACATGCCAGTTTTCGCCGCCAACCTGACGATGATGTTCAGCGAATGGGACTTCCTCGACCGCTTCGACGCC
GCAGCCGACGCGGGGTTTTC

Product: putative 3-hydroxyisobutyrate dehydrogenase protein

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTPLAENSGSAIVAAVIGLGSMGMGMASSMKRAGLDVVGYDITPAAVDRFVAEGGRGAASPADAAKGADIIVSVVVNGAQ
TEAVLFGSKGVAAPMKRGAVFISSATMDPAIARDLAARAQALGLHYLDAPISGGAARAAKGELTIMASGSRQAFDRARPA
LDAMAGKVYELGEAAGTGAAFKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYT
PASSIEIFVKDLGIVLDMARSQRYPAPLAAAALQMYLAAAGAGMGGDDDSSLARLYAQLSGAQLPGFAKEPQGR

Sequences:

>Translated_314_residues
MTPLAENSGSAIVAAVIGLGSMGMGMASSMKRAGLDVVGYDITPAAVDRFVAEGGRGAASPADAAKGADIIVSVVVNGAQ
TEAVLFGSKGVAAPMKRGAVFISSATMDPAIARDLAARAQALGLHYLDAPISGGAARAAKGELTIMASGSRQAFDRARPA
LDAMAGKVYELGEAAGTGAAFKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYT
PASSIEIFVKDLGIVLDMARSQRYPAPLAAAALQMYLAAAGAGMGGDDDSSLARLYAQLSGAQLPGFAKEPQGR
>Mature_313_residues
TPLAENSGSAIVAAVIGLGSMGMGMASSMKRAGLDVVGYDITPAAVDRFVAEGGRGAASPADAAKGADIIVSVVVNGAQT
EAVLFGSKGVAAPMKRGAVFISSATMDPAIARDLAARAQALGLHYLDAPISGGAARAAKGELTIMASGSRQAFDRARPAL
DAMAGKVYELGEAAGTGAAFKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYTP
ASSIEIFVKDLGIVLDMARSQRYPAPLAAAALQMYLAAAGAGMGGDDDSSLARLYAQLSGAQLPGFAKEPQGR

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=31.8339100346021, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=26.0714285714286, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1789092, Length=300, Percent_Identity=48.3333333333333, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI145693186, Length=288, Percent_Identity=32.6388888888889, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1790315, Length=279, Percent_Identity=35.1254480286738, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786719, Length=288, Percent_Identity=29.1666666666667, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17557316, Length=268, Percent_Identity=31.7164179104478, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24655230, Length=289, Percent_Identity=30.1038062283737, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922568, Length=289, Percent_Identity=30.1038062283737, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574115, Length=278, Percent_Identity=24.1007194244604, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31821; Mature: 31690

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLAENSGSAIVAAVIGLGSMGMGMASSMKRAGLDVVGYDITPAAVDRFVAEGGRGAAS
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCC
PADAAKGADIIVSVVVNGAQTEAVLFGSKGVAAPMKRGAVFISSATMDPAIARDLAARAQ
CCHHHCCCCEEEEEEECCCCCEEEEECCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHH
ALGLHYLDAPISGGAARAAKGELTIMASGSRQAFDRARPALDAMAGKVYELGEAAGTGAA
HHHHHEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEEECCCC
PASSIEIFVKDLGIVLDMARSQRYPAPLAAAALQMYLAAAGAGMGGDDDSSLARLYAQLS
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GAQLPGFAKEPQGR
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TPLAENSGSAIVAAVIGLGSMGMGMASSMKRAGLDVVGYDITPAAVDRFVAEGGRGAAS
CCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCC
PADAAKGADIIVSVVVNGAQTEAVLFGSKGVAAPMKRGAVFISSATMDPAIARDLAARAQ
CCHHHCCCCEEEEEEECCCCCEEEEECCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHH
ALGLHYLDAPISGGAARAAKGELTIMASGSRQAFDRARPALDAMAGKVYELGEAAGTGAA
HHHHHEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEEECCCC
PASSIEIFVKDLGIVLDMARSQRYPAPLAAAALQMYLAAAGAGMGGDDDSSLARLYAQLS
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GAQLPGFAKEPQGR
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]