Click here to switch to the map view.

The map label for this gene is ycdJ [C]

Identifier: 86360998

GI number: 86360998

Start: 291916

End: 292779

Strand: Direct

Name: ycdJ [C]

Synonym: RHE_PF00268

Alternate gene names: 86360998

Gene position: 291916-292779 (Clockwise)

Preceding gene: 86360997

Following gene: 86360999

Centisome position: 45.43

GC content: 61.46

Gene sequence:

>864_bases
GTGTTTGAAGGCTTTTCTCTCGAGGCGGTCGATGTCGGGCCGGGATCGCTTCGCGTCCGCCGCGGCGGCTCAGGACCCGC
CGTTCTTCTGCTTCACGGCCACCCCAGGACACATATGACCTGGGGCAAGGTGGCGGACCTGCTTTCACCCGATCACACGG
TCGTCTGCCCCGATCTCCCCGGCTTCGGCCGCTCCTATCAGCCCGGCGATGCTTCCGACAGCAGAAATTCTTCCAAGCGA
GCTAAGGCCGAAGCGCACATCGAGCTGATGCGGCGACTGGGTCACGAGAACTTCGCGGTGGTCGGCCATGACCGCGGGAG
CCTCACTGCCTTCCGCATGGCAATGGACCATCCAGATCGCGTAAGGAAACTCGTCATTGTCGACGCCATTCCCGTCATCG
AACATCTCGAACGTGCCGACTGGAAATTTGCGCGGGACTGGTACCACTGGTTCTTCTTCGCCCAGAAGGAAAGACCGGAG
CGGGCGATCTCCGCCGATCCCCTCGCGTGGTACGACAAACTTTCGCCCGCGCTAATGGGCCCCCAAGCCTATGAAGATCT
CATCGACGTCATCCACGATCCTCACGTCATCCACGGGATGATCGAGGATTACCGCGCCAGCCTCAGCATCGACCATCTGC
ACGACGGTGATGACCGCGCCGCCGGCCGCAAAATAATCTGCCCGATGCTCTGCCTCTGGTCGCTGCGCGACGATATGGAG
CAGATCTATGGCGATCCAGTCGCGATCTGGCGCAACTGGGCTAGGGACGTGCGCGGCTTCGGCATAGACAGTGGACACCA
TGTGGCCGAGGAGAATCCGGCAGCACTCTCGCAGGCCATCCGGGAGTTTCTCGAAAACGGATAG

Upstream 100 bases:

>100_bases
TTCCGATTCGCGTGCTGCAGCTTGCCGGCGAAGCCGGCAGGAAGGGCGAAGGGGCGACATTTTCGGACTATGCCAAGCTC
GTTGGAAAGCTGAGCTAAGC

Downstream 100 bases:

>100_bases
GGTGAAGGCCCAAAGCGCTCTCCATCCCGCTAGGAATGTCCCGTTCTGACGATTTCTGCGATAAGTTGATAAATTTGGTC
TTGTTTCCGAGGCGCTCCTA

Product: putative hydrolase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MFEGFSLEAVDVGPGSLRVRRGGSGPAVLLLHGHPRTHMTWGKVADLLSPDHTVVCPDLPGFGRSYQPGDASDSRNSSKR
AKAEAHIELMRRLGHENFAVVGHDRGSLTAFRMAMDHPDRVRKLVIVDAIPVIEHLERADWKFARDWYHWFFFAQKERPE
RAISADPLAWYDKLSPALMGPQAYEDLIDVIHDPHVIHGMIEDYRASLSIDHLHDGDDRAAGRKIICPMLCLWSLRDDME
QIYGDPVAIWRNWARDVRGFGIDSGHHVAEENPAALSQAIREFLENG

Sequences:

>Translated_287_residues
MFEGFSLEAVDVGPGSLRVRRGGSGPAVLLLHGHPRTHMTWGKVADLLSPDHTVVCPDLPGFGRSYQPGDASDSRNSSKR
AKAEAHIELMRRLGHENFAVVGHDRGSLTAFRMAMDHPDRVRKLVIVDAIPVIEHLERADWKFARDWYHWFFFAQKERPE
RAISADPLAWYDKLSPALMGPQAYEDLIDVIHDPHVIHGMIEDYRASLSIDHLHDGDDRAAGRKIICPMLCLWSLRDDME
QIYGDPVAIWRNWARDVRGFGIDSGHHVAEENPAALSQAIREFLENG
>Mature_287_residues
MFEGFSLEAVDVGPGSLRVRRGGSGPAVLLLHGHPRTHMTWGKVADLLSPDHTVVCPDLPGFGRSYQPGDASDSRNSSKR
AKAEAHIELMRRLGHENFAVVGHDRGSLTAFRMAMDHPDRVRKLVIVDAIPVIEHLERADWKFARDWYHWFFFAQKERPE
RAISADPLAWYDKLSPALMGPQAYEDLIDVIHDPHVIHGMIEDYRASLSIDHLHDGDDRAAGRKIICPMLCLWSLRDDME
QIYGDPVAIWRNWARDVRGFGIDSGHHVAEENPAALSQAIREFLENG

Specific function: Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has low activity towards chloroacetate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324392, Length=277, Percent_Identity=25.2707581227437, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.3 [H]

Molecular weight: Translated: 32391; Mature: 32391

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEGFSLEAVDVGPGSLRVRRGGSGPAVLLLHGHPRTHMTWGKVADLLSPDHTVVCPDLP
CCCCCEEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEECCCCC
GFGRSYQPGDASDSRNSSKRAKAEAHIELMRRLGHENFAVVGHDRGSLTAFRMAMDHPDR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHH
VRKLVIVDAIPVIEHLERADWKFARDWYHWFFFAQKERPERAISADPLAWYDKLSPALMG
HHEEHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHCCHHHCC
PQAYEDLIDVIHDPHVIHGMIEDYRASLSIDHLHDGDDRAAGRKIICPMLCLWSLRDDME
HHHHHHHHHHHCCCHHHHHHHHHHHHHCEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHH
QIYGDPVAIWRNWARDVRGFGIDSGHHVAEENPAALSQAIREFLENG
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEGFSLEAVDVGPGSLRVRRGGSGPAVLLLHGHPRTHMTWGKVADLLSPDHTVVCPDLP
CCCCCEEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEECCCCC
GFGRSYQPGDASDSRNSSKRAKAEAHIELMRRLGHENFAVVGHDRGSLTAFRMAMDHPDR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHH
VRKLVIVDAIPVIEHLERADWKFARDWYHWFFFAQKERPERAISADPLAWYDKLSPALMG
HHEEHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHCCHHHCC
PQAYEDLIDVIHDPHVIHGMIEDYRASLSIDHLHDGDDRAAGRKIICPMLCLWSLRDDME
HHHHHHHHHHHCCCHHHHHHHHHHHHHCEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHH
QIYGDPVAIWRNWARDVRGFGIDSGHHVAEENPAALSQAIREFLENG
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA