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The map label for this gene is yogA [H]

Identifier: 86360934

GI number: 86360934

Start: 214238

End: 215311

Strand: Direct

Name: yogA [H]

Synonym: RHE_PF00203

Alternate gene names: 86360934

Gene position: 214238-215311 (Clockwise)

Preceding gene: 86360933

Following gene: 86360935

Centisome position: 33.34

GC content: 63.87

Gene sequence:

>1074_bases
ATGACGATCCCGGAAAAGATGGCGGCGGTGTTGCTCACCGGCCATGGCGGCCTGGAAAAGCTCGTCTACGACAGGGACTT
TCCTGTTCCGACACCGGCGGAGGGCGAGGTGCTGATCCGCGTTACCGCCTGCGGCATGAACAATACCGACGTCTGGGTGC
GCCAAGGCGCCTACGGCACAGAGGATGATCCCTCCGCCGTCTCCACCTGGCGCAGGCAGGGCAACACGCTGACCTTCCCG
CGCATCCAGGGCACCGATACGGTCGGTCATATCGTCGGTGCCGGCAAAGGCGTCGATCCGAAGCGCATCGGCGAACGCGT
CATGGTCGATTTCTCGATCTACAATCGCGACGACGACAGTCTTGCCGACATCGATTACATGGGCCACGGGCGGGATGGCG
GCTACGCCGAATATATGGTGCTGCCTGCCGAGAACGCCCATGTCGTCGCGACCGATCTGACCGATATCGAGCTCGCCACT
TTCTGCTGCGCCTATCTCACAGGCGAACGGATGCTGGAACGGGCAAGGCTTGCCGCCGGCGAACGCGTTCTCGTCACCGG
CGCTTCCGGCGGCGTCGGTTCAGCGATCATCCAGCTCGCCCGTGCCCGCGGCGCCATCCCGATCGCGGTCGCAGGCCCAG
GCAAAGAGGCGGCGATGCTCGAGATCGGCGCCGAAGCGGTCGTCACTCGCGGCAAGGGCGATCTGGTCGAAGCCGTCCAT
TCGGCAAGCCGCGGACAGCCGATCGATGTCGTCGCCGATCTCGTCGGCGGGCCGCTGTTCAACGATCTCCTGAAGATCCT
GCGTCCCGAGGGCCGTTACACGACGGCCGGCGCGATCGCCGGGCCCGTCGTCCAGCTCGACCTCAGGACCATGTACCTGA
AGCATTTGGAACTGCATGGATCGAGCCAGGGAAGCCGCGCCGACTTCCACCGGCTGGTCGGCTATATCGAAAACCGCAAG
ATCCGGCCGCTCGTCGGCGGTGTTTATCCGCTGTCGGAATTTCATCGCGCCCAGACCGATTTCATGGCGAAGAACTTCGT
CGGCAAATTGGTCGTGGTACCCGACCAGAGATAG

Upstream 100 bases:

>100_bases
CCACCACTCTGCCTCACCCGCGAAGAAGCAGACGTCGTCGTCAAGGCGGCTGCGGACGCGATCGAAAGTGTTTTCAAAAC
CATCTGAGGAGAAGAGCCGC

Downstream 100 bases:

>100_bases
TGCGCTTCCCCGATCACGCCGCCGGCGAACCTTATCGGTTTAGCGGCGTTTCACGGCCATGGACACCGCCAAAACCACAT
ATCTCACCCCTGCCGAACTC

Product: Zn-dependent oxidoreductase protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTIPEKMAAVLLTGHGGLEKLVYDRDFPVPTPAEGEVLIRVTACGMNNTDVWVRQGAYGTEDDPSAVSTWRRQGNTLTFP
RIQGTDTVGHIVGAGKGVDPKRIGERVMVDFSIYNRDDDSLADIDYMGHGRDGGYAEYMVLPAENAHVVATDLTDIELAT
FCCAYLTGERMLERARLAAGERVLVTGASGGVGSAIIQLARARGAIPIAVAGPGKEAAMLEIGAEAVVTRGKGDLVEAVH
SASRGQPIDVVADLVGGPLFNDLLKILRPEGRYTTAGAIAGPVVQLDLRTMYLKHLELHGSSQGSRADFHRLVGYIENRK
IRPLVGGVYPLSEFHRAQTDFMAKNFVGKLVVVPDQR

Sequences:

>Translated_357_residues
MTIPEKMAAVLLTGHGGLEKLVYDRDFPVPTPAEGEVLIRVTACGMNNTDVWVRQGAYGTEDDPSAVSTWRRQGNTLTFP
RIQGTDTVGHIVGAGKGVDPKRIGERVMVDFSIYNRDDDSLADIDYMGHGRDGGYAEYMVLPAENAHVVATDLTDIELAT
FCCAYLTGERMLERARLAAGERVLVTGASGGVGSAIIQLARARGAIPIAVAGPGKEAAMLEIGAEAVVTRGKGDLVEAVH
SASRGQPIDVVADLVGGPLFNDLLKILRPEGRYTTAGAIAGPVVQLDLRTMYLKHLELHGSSQGSRADFHRLVGYIENRK
IRPLVGGVYPLSEFHRAQTDFMAKNFVGKLVVVPDQR
>Mature_356_residues
TIPEKMAAVLLTGHGGLEKLVYDRDFPVPTPAEGEVLIRVTACGMNNTDVWVRQGAYGTEDDPSAVSTWRRQGNTLTFPR
IQGTDTVGHIVGAGKGVDPKRIGERVMVDFSIYNRDDDSLADIDYMGHGRDGGYAEYMVLPAENAHVVATDLTDIELATF
CCAYLTGERMLERARLAAGERVLVTGASGGVGSAIIQLARARGAIPIAVAGPGKEAAMLEIGAEAVVTRGKGDLVEAVHS
ASRGQPIDVVADLVGGPLFNDLLKILRPEGRYTTAGAIAGPVVQLDLRTMYLKHLELHGSSQGSRADFHRLVGYIENRKI
RPLVGGVYPLSEFHRAQTDFMAKNFVGKLVVVPDQR

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=363, Percent_Identity=27.5482093663912, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI22538444, Length=363, Percent_Identity=27.5482093663912, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI24308257, Length=376, Percent_Identity=27.3936170212766, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI194239674, Length=356, Percent_Identity=25.2808988764045, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI13236495, Length=356, Percent_Identity=25.2808988764045, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI18379349, Length=375, Percent_Identity=25.3333333333333, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI194239676, Length=266, Percent_Identity=29.3233082706767, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI28557745, Length=289, Percent_Identity=26.2975778546713, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1790485, Length=361, Percent_Identity=24.9307479224377, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI226510992, Length=168, Percent_Identity=25.5952380952381, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787863, Length=181, Percent_Identity=27.0718232044199, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=331, Percent_Identity=26.5861027190332, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17562582, Length=321, Percent_Identity=25.8566978193146, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562584, Length=325, Percent_Identity=24.9230769230769, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=364, Percent_Identity=25, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323961, Length=324, Percent_Identity=29.0123456790123, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=324, Percent_Identity=29.6296296296296, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=362, Percent_Identity=26.2430939226519, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319621, Length=318, Percent_Identity=27.6729559748428, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 38362; Mature: 38231

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPEKMAAVLLTGHGGLEKLVYDRDFPVPTPAEGEVLIRVTACGMNNTDVWVRQGAYGT
CCCHHHEEEEEEECCCCHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCC
EDDPSAVSTWRRQGNTLTFPRIQGTDTVGHIVGAGKGVDPKRIGERVMVDFSIYNRDDDS
CCCHHHHHHHHHCCCEEECCCCCCCCCHHHHEECCCCCCHHHCCCEEEEEEEEECCCCCC
LADIDYMGHGRDGGYAEYMVLPAENAHVVATDLTDIELATFCCAYLTGERMLERARLAAG
CCCEEECCCCCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ERVLVTGASGGVGSAIIQLARARGAIPIAVAGPGKEAAMLEIGAEAVVTRGKGDLVEAVH
CEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHEEECCCCHHHHHHH
SASRGQPIDVVADLVGGPLFNDLLKILRPEGRYTTAGAIAGPVVQLDLRTMYLKHLELHG
HHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHCC
SSQGSRADFHRLVGYIENRKIRPLVGGVYPLSEFHRAQTDFMAKNFVGKLVVVPDQR
CCCCCHHHHHHHHHHHCCCEECHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCC
>Mature Secondary Structure 
TIPEKMAAVLLTGHGGLEKLVYDRDFPVPTPAEGEVLIRVTACGMNNTDVWVRQGAYGT
CCHHHEEEEEEECCCCHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCC
EDDPSAVSTWRRQGNTLTFPRIQGTDTVGHIVGAGKGVDPKRIGERVMVDFSIYNRDDDS
CCCHHHHHHHHHCCCEEECCCCCCCCCHHHHEECCCCCCHHHCCCEEEEEEEEECCCCCC
LADIDYMGHGRDGGYAEYMVLPAENAHVVATDLTDIELATFCCAYLTGERMLERARLAAG
CCCEEECCCCCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ERVLVTGASGGVGSAIIQLARARGAIPIAVAGPGKEAAMLEIGAEAVVTRGKGDLVEAVH
CEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHEEECCCCHHHHHHH
SASRGQPIDVVADLVGGPLFNDLLKILRPEGRYTTAGAIAGPVVQLDLRTMYLKHLELHG
HHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHCC
SSQGSRADFHRLVGYIENRKIRPLVGGVYPLSEFHRAQTDFMAKNFVGKLVVVPDQR
CCCCCHHHHHHHHHHHCCCEECHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]