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The map label for this gene is napG [H]

Identifier: 85861008

GI number: 85861008

Start: 3172063

End: 3173649

Strand: Direct

Name: napG [H]

Synonym: SYN_02058

Alternate gene names: 85861008

Gene position: 3172063-3173649 (Clockwise)

Preceding gene: 85861007

Following gene: 85861012

Centisome position: 99.77

GC content: 59.17

Gene sequence:

>1587_bases
ATGCAGGCGCCCGACTTTCCTGCACGACATCAAAGCCCGATGCTTAAAAATATCCGACGCCTGACTCAGGCCATCTTTCT
GCTTCTTTTTCTCTGGCTTTTCCTCTCGACGGAATCCCGGGGGGCCAATGAACTGGGCTACCCGGTCAAAATCTTCCTTG
ACGCCGATCCGCTTCTCACCCTCACCACGATCCTGTCCACGCGGAGGCTGGACAACCCTTTCCTGCTGGCCCTGTTCGTT
CTGATCGCCACGGTTGTGCTGGGCCGGGTGTTCTGCGGCTGGATCTGCCCGCTGGGAACCCTGCACAACATGGTAAGTTC
GCTGAAGAGACGGAACGTCACCGGGAAACCCCGCAATCTCTATTTATGGAAATATCTCCTGCTGATCTTTCTGCTGGTTT
CCTCGGCCTTTTCCCTTCAGCTTGCCGGGATCGCAGATCCTCTTTCCCTGCTGATCCGATCGTTGACGCTGGCCGTTTAC
CCCCTGATCCAGTATGCGACCGTCAGCGTCTTCGACACCGTCTATGCCTGGAATTTCCCTGTCGTTACCGATATTTCCGA
GTTTATTTATGATCTGCTGAAAAAGACCTTCCTCTCTTTCCACCAGCCCCATTTTCAGCAGTCTGTCCTGGTCGGCCTGC
TGTTCTTCGGTATCCTGGCCCTGAATCTTGCGGAAAAACGCTTCTGGTGCAAATACCTCTGTCCGCTGGGGGCCCTGCTC
GGGCTCTGTTCCCGATACGCCTTGCTGAACCTCTCCGTCAGTGAATCCTGCAACGGCTGCGGATCCTGCAGCGAGGTCTG
CCCGGGCGGAGCTTTGCCGGATTCGAGGAAAAACTGGCGCAAGCCGGAATGCCTGGTCTGCATGAACTGTGATGATCTGT
GCCCCCGGAATGCCGTCCGCTTCGGATTTTCCCGGAAACCATCCGGGGCGGCGCTGGATCTGGGAAAGCGACGGGTTATC
GGATCGATTGTCGCCGGGCTGGTCGCCGTCCCCCTGTTCAAAGTTTCGCCGCTGAAAAAAGCCGGTGCCGCTGAACCCCG
GTTGATCCGTCCCCCAGGTTCCCTGGAAGAAACGGAGTTTTTGAAACGATGCATCAAATGCGGGCAGTGCATAAAGGTCT
GCATCACGGGCGGCCTCCAGCCCACCCTGCTGGAAGCGGGACTGGACGGGATCTGGTCGCCGGTTCTCGTACCCAGGATC
GGCTACTGCGAATACCGGTGCACCCTCTGCGGGCAGGTCTGCCCGACAGGCGCGATTCGAGAACTTGCCCTGGAGGAAAA
GGCAGCGGTCCGGATCGGAACAGCCATGATCGACAAGGGGCGCTGTCTTCCTTTCGCGCATGCAACCCCCTGCATCGTCT
GCCAGGAGGTCTGCCCCACCCCGCAAAAGGCGATCTGGCTTGAGGCCGTGCGGGTGAAAAACAGGACCGGCCGGACCATG
ACCCTCCGACAGCCCCATGTGGATCTGGAACTCTGCGTCGGCTGCGGCATCTGCGAAGCCAAATGCCCGGTCCTCGGTCA
ACCCGCCATCAGGGCGACCAGCATCGGGGAATCCCGGTCGCAGGACAACCAGCTCCTGCTGCCCTGA

Upstream 100 bases:

>100_bases
CTGGGTTTTGTCCGCATCGGCCACCAGCGCCGACTGGGAAGCATGGACCTGGCCGGAATGAACATCAGGAGAATTACCCT
GTAAGCATGTCGCCACCTGC

Downstream 100 bases:

>100_bases
TCTTCAGGAATCTCCCTCTTCCCGGACTCATGAAGGCGCGGAACGTTTTGCATCTCCAGCAAAGTTGCATTCCATATGCA
ATATTTAATAAGGATGACAA

Product: 4Fe-4S binding protein

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MQAPDFPARHQSPMLKNIRRLTQAIFLLLFLWLFLSTESRGANELGYPVKIFLDADPLLTLTTILSTRRLDNPFLLALFV
LIATVVLGRVFCGWICPLGTLHNMVSSLKRRNVTGKPRNLYLWKYLLLIFLLVSSAFSLQLAGIADPLSLLIRSLTLAVY
PLIQYATVSVFDTVYAWNFPVVTDISEFIYDLLKKTFLSFHQPHFQQSVLVGLLFFGILALNLAEKRFWCKYLCPLGALL
GLCSRYALLNLSVSESCNGCGSCSEVCPGGALPDSRKNWRKPECLVCMNCDDLCPRNAVRFGFSRKPSGAALDLGKRRVI
GSIVAGLVAVPLFKVSPLKKAGAAEPRLIRPPGSLEETEFLKRCIKCGQCIKVCITGGLQPTLLEAGLDGIWSPVLVPRI
GYCEYRCTLCGQVCPTGAIRELALEEKAAVRIGTAMIDKGRCLPFAHATPCIVCQEVCPTPQKAIWLEAVRVKNRTGRTM
TLRQPHVDLELCVGCGICEAKCPVLGQPAIRATSIGESRSQDNQLLLP

Sequences:

>Translated_528_residues
MQAPDFPARHQSPMLKNIRRLTQAIFLLLFLWLFLSTESRGANELGYPVKIFLDADPLLTLTTILSTRRLDNPFLLALFV
LIATVVLGRVFCGWICPLGTLHNMVSSLKRRNVTGKPRNLYLWKYLLLIFLLVSSAFSLQLAGIADPLSLLIRSLTLAVY
PLIQYATVSVFDTVYAWNFPVVTDISEFIYDLLKKTFLSFHQPHFQQSVLVGLLFFGILALNLAEKRFWCKYLCPLGALL
GLCSRYALLNLSVSESCNGCGSCSEVCPGGALPDSRKNWRKPECLVCMNCDDLCPRNAVRFGFSRKPSGAALDLGKRRVI
GSIVAGLVAVPLFKVSPLKKAGAAEPRLIRPPGSLEETEFLKRCIKCGQCIKVCITGGLQPTLLEAGLDGIWSPVLVPRI
GYCEYRCTLCGQVCPTGAIRELALEEKAAVRIGTAMIDKGRCLPFAHATPCIVCQEVCPTPQKAIWLEAVRVKNRTGRTM
TLRQPHVDLELCVGCGICEAKCPVLGQPAIRATSIGESRSQDNQLLLP
>Mature_528_residues
MQAPDFPARHQSPMLKNIRRLTQAIFLLLFLWLFLSTESRGANELGYPVKIFLDADPLLTLTTILSTRRLDNPFLLALFV
LIATVVLGRVFCGWICPLGTLHNMVSSLKRRNVTGKPRNLYLWKYLLLIFLLVSSAFSLQLAGIADPLSLLIRSLTLAVY
PLIQYATVSVFDTVYAWNFPVVTDISEFIYDLLKKTFLSFHQPHFQQSVLVGLLFFGILALNLAEKRFWCKYLCPLGALL
GLCSRYALLNLSVSESCNGCGSCSEVCPGGALPDSRKNWRKPECLVCMNCDDLCPRNAVRFGFSRKPSGAALDLGKRRVI
GSIVAGLVAVPLFKVSPLKKAGAAEPRLIRPPGSLEETEFLKRCIKCGQCIKVCITGGLQPTLLEAGLDGIWSPVLVPRI
GYCEYRCTLCGQVCPTGAIRELALEEKAAVRIGTAMIDKGRCLPFAHATPCIVCQEVCPTPQKAIWLEAVRVKNRTGRTM
TLRQPHVDLELCVGCGICEAKCPVLGQPAIRATSIGESRSQDNQLLLP

Specific function: Involved in electron transfer (Probable) [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788533, Length=202, Percent_Identity=37.1287128712871, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787226, Length=251, Percent_Identity=27.4900398406374, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004494
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 58218; Mature: 58218

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEECCCCCHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure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EEECCCCCHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]