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The map label for this gene is rnfE [H]

Identifier: 85860865

GI number: 85860865

Start: 3026894

End: 3027538

Strand: Direct

Name: rnfE [H]

Synonym: SYN_01661

Alternate gene names: 85860865

Gene position: 3026894-3027538 (Clockwise)

Preceding gene: 85860864

Following gene: 85860866

Centisome position: 95.21

GC content: 50.39

Gene sequence:

>645_bases
TTGACATTAAGGAGGAGTATTTTGGCCGCCACGAAAAAAACCAATAGCGAACTGCTTTTCAACGGGATTTTGCTGGAAAA
TCCGATTTTCCGCCTGGCCCTTTCCATGTGCCCGGCCGTCGGATTGACGACCTCTATCAAAAACGGTCTGCTGCTCGGCA
CCGCCGTCCTTTTCGTCCAGGTCTTTTCCAGTTGTACGATTGCCGCCATCAAGAACTTTATTCACCCCAGGATTCGCATT
CCGACCTACACGTTGACCATTGCCACCTGGGTTACGGTCATCGACCTGGTCCTGGCTGCCTATGTGCCCGAAGCCTACAA
GCAGGTGGGACTTTTCGTCAAACTGATTGTTGCGTTTGCAATTATTACCATGCGGCTTGAAATGTTTGCCTGTAAGAACA
CTGTTACGTCCTCATTCTTTGATGGTGTCGGCATGGGCATCGGGTTCATGGTCGGGATGGTGACTATCGGCTTCGTTCGG
GAGCTTCTCGGCGCAGGTACGCTTCTCGGCTACGATGTTCTTGGATTCAAACCCCTGCTTTTCTTTGTTCTCCCTGCTGC
AGGTTTCTTCTGTGTGGGGATCATGATGGCAATGTTCAACTACATTGAGGTGGTTTTCAACCGCAGTAAAGAGAGGAAGT
CATGA

Upstream 100 bases:

>100_bases
GGTGCGTGACAGATGGCGTGAAGAACATGGCCAAGCGGTTTGCCTACCGGATCAAGATCCTGGATGAGGCAATCGCCAGC
CAGAAGATACCAGCTTTGAT

Downstream 100 bases:

>100_bases
GCACTTTGATGAAAAGATCCATTTTGCTGTCGATCTGGCTGGTTTCGATCTTTCTTGCCAGTCTGCCGGTGTGCGGTCAT
GCTGCCGAAAATTCATTTGC

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfE [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTLRRSILAATKKTNSELLFNGILLENPIFRLALSMCPAVGLTTSIKNGLLLGTAVLFVQVFSSCTIAAIKNFIHPRIRI
PTYTLTIATWVTVIDLVLAAYVPEAYKQVGLFVKLIVAFAIITMRLEMFACKNTVTSSFFDGVGMGIGFMVGMVTIGFVR
ELLGAGTLLGYDVLGFKPLLFFVLPAAGFFCVGIMMAMFNYIEVVFNRSKERKS

Sequences:

>Translated_214_residues
MTLRRSILAATKKTNSELLFNGILLENPIFRLALSMCPAVGLTTSIKNGLLLGTAVLFVQVFSSCTIAAIKNFIHPRIRI
PTYTLTIATWVTVIDLVLAAYVPEAYKQVGLFVKLIVAFAIITMRLEMFACKNTVTSSFFDGVGMGIGFMVGMVTIGFVR
ELLGAGTLLGYDVLGFKPLLFFVLPAAGFFCVGIMMAMFNYIEVVFNRSKERKS
>Mature_213_residues
TLRRSILAATKKTNSELLFNGILLENPIFRLALSMCPAVGLTTSIKNGLLLGTAVLFVQVFSSCTIAAIKNFIHPRIRIP
TYTLTIATWVTVIDLVLAAYVPEAYKQVGLFVKLIVAFAIITMRLEMFACKNTVTSSFFDGVGMGIGFMVGMVTIGFVRE
LLGAGTLLGYDVLGFKPLLFFVLPAAGFFCVGIMMAMFNYIEVVFNRSKERKS

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=196, Percent_Identity=30.6122448979592, Blast_Score=84, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 23477; Mature: 23346

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRRSILAATKKTNSELLFNGILLENPIFRLALSMCPAVGLTTSIKNGLLLGTAVLFVQ
CCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHCHHCCHHHHHCCHHHHHHHHHHH
VFSSCTIAAIKNFIHPRIRIPTYTLTIATWVTVIDLVLAAYVPEAYKQVGLFVKLIVAFA
HHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IITMRLEMFACKNTVTSSFFDGVGMGIGFMVGMVTIGFVRELLGAGTLLGYDVLGFKPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FFVLPAAGFFCVGIMMAMFNYIEVVFNRSKERKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
TLRRSILAATKKTNSELLFNGILLENPIFRLALSMCPAVGLTTSIKNGLLLGTAVLFVQ
CHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHCHHCCHHHHHCCHHHHHHHHHHH
VFSSCTIAAIKNFIHPRIRIPTYTLTIATWVTVIDLVLAAYVPEAYKQVGLFVKLIVAFA
HHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IITMRLEMFACKNTVTSSFFDGVGMGIGFMVGMVTIGFVRELLGAGTLLGYDVLGFKPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FFVLPAAGFFCVGIMMAMFNYIEVVFNRSKERKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA