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The map label for this gene is def [H]

Identifier: 85860534

GI number: 85860534

Start: 2714698

End: 2715267

Strand: Direct

Name: def [H]

Synonym: SYN_01803

Alternate gene names: 85860534

Gene position: 2714698-2715267 (Clockwise)

Preceding gene: 85860515

Following gene: 85860535

Centisome position: 85.39

GC content: 51.93

Gene sequence:

>570_bases
ATGTCTTATCGTCACAAAGTATTAACCCTCTGGAGCGAAATTTCCGTCAACGATGAAGAAACGCGCATTCTGCGGACCCC
CTCCAGGGATCTCCCGATTCCGCTGAGCCGCGAGGCCAGAGACCAGATACAAACTCTGGTTGACGCCTTTCTTGAGCGGG
ATGACGCACTGGGATTGGCAGCGCCTCAGATCGGAATAAACCGGAGGATCGTAATCTTCCGCAACAAGGGATTTGATGAG
GAAGGGTGGTCCAAAAAAGAAAAAGACTATGATCTGCTGATCAACCCCCGTATCACCCAGACCAGGGGAGAGCTTGTCAA
AGGAGCGGAGGGATGTCTCTCCTGCCCGAGTATTCAGGTGGAAGTCAACCGTTTCCCGGAAGTTAAAGTTCGGGCCTTCG
ATCGTCACGGCAACCGGATCAGCAAACGGTATGCCGATTTTCTCGCCCGCGTCGCTCAACACGAACTCGACCATCTGGAA
GGAAAATTGATTGTGGATTGTGAAGGCCCGGTCTACTTTCCTCGAGAGAAAAAAGCCTTCTTTGAACGGATTTTTGCGCA
GATTCCCTGA

Upstream 100 bases:

>100_bases
TATAAGATATTATCAAGGATATTGTCATCATGCAAATCCTTGAAATGGGCAGGTCAACATGTTATAGAGCTTTGAAAAAG
ATTATTAGAGGCTGTATTTC

Downstream 100 bases:

>100_bases
CACCGGGCTGCATTTTCAAAGGATAACAAAGCGGCGGAGAATAAGCGTCGCAGGCATATGAGAGCATATGCCGTGAAACC
AATGCGAAACCGATGAAAGT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSYRHKVLTLWSEISVNDEETRILRTPSRDLPIPLSREARDQIQTLVDAFLERDDALGLAAPQIGINRRIVIFRNKGFDE
EGWSKKEKDYDLLINPRITQTRGELVKGAEGCLSCPSIQVEVNRFPEVKVRAFDRHGNRISKRYADFLARVAQHELDHLE
GKLIVDCEGPVYFPREKKAFFERIFAQIP

Sequences:

>Translated_189_residues
MSYRHKVLTLWSEISVNDEETRILRTPSRDLPIPLSREARDQIQTLVDAFLERDDALGLAAPQIGINRRIVIFRNKGFDE
EGWSKKEKDYDLLINPRITQTRGELVKGAEGCLSCPSIQVEVNRFPEVKVRAFDRHGNRISKRYADFLARVAQHELDHLE
GKLIVDCEGPVYFPREKKAFFERIFAQIP
>Mature_188_residues
SYRHKVLTLWSEISVNDEETRILRTPSRDLPIPLSREARDQIQTLVDAFLERDDALGLAAPQIGINRRIVIFRNKGFDEE
GWSKKEKDYDLLINPRITQTRGELVKGAEGCLSCPSIQVEVNRFPEVKVRAFDRHGNRISKRYADFLARVAQHELDHLEG
KLIVDCEGPVYFPREKKAFFERIFAQIP

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=137, Percent_Identity=35.7664233576642, Blast_Score=74, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24645726, Length=145, Percent_Identity=35.1724137931034, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645728, Length=155, Percent_Identity=29.0322580645161, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21906; Mature: 21775

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRHKVLTLWSEISVNDEETRILRTPSRDLPIPLSREARDQIQTLVDAFLERDDALGLA
CCCCHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
APQIGINRRIVIFRNKGFDEEGWSKKEKDYDLLINPRITQTRGELVKGAEGCLSCPSIQV
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCHHHCCCCCEEE
EVNRFPEVKVRAFDRHGNRISKRYADFLARVAQHELDHLEGKLIVDCEGPVYFPREKKAF
EECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCHHHHH
FERIFAQIP
HHHHHHHCC
>Mature Secondary Structure 
SYRHKVLTLWSEISVNDEETRILRTPSRDLPIPLSREARDQIQTLVDAFLERDDALGLA
CCCHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
APQIGINRRIVIFRNKGFDEEGWSKKEKDYDLLINPRITQTRGELVKGAEGCLSCPSIQV
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCHHHCCCCCEEE
EVNRFPEVKVRAFDRHGNRISKRYADFLARVAQHELDHLEGKLIVDCEGPVYFPREKKAF
EECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCHHHHH
FERIFAQIP
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]