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The map label for this gene is rutB [H]

Identifier: 85860360

GI number: 85860360

Start: 2538525

End: 2538917

Strand: Direct

Name: rutB [H]

Synonym: SYN_01201

Alternate gene names: 85860360

Gene position: 2538525-2538917 (Clockwise)

Preceding gene: 85860359

Following gene: 85860362

Centisome position: 79.85

GC content: 56.74

Gene sequence:

>393_bases
ATGCCCGATGATTTTCTTTTCAAGTCCCGAGCGAAGCCGCATGGAATTCGTGGAAAGGCCGGAGCCCAGGTCATCTCCGA
ACTGAAACCCGAAAACGGCGACATCATCGTCCCCAAACCCCGTTTCAGCGCTTTTTTCAAGACGGACCTCGACCGGATCC
TGGGAGAACGGGGGGTGGACACCGTCGTGATCGGCGGGGTCGCCACCGAGGTATGCGTTCTCAGCACGGCCTATGATGCC
GTATGCCATAATTTCACCGTCATCGTCCTTGAGGATTGCTGCGCCTCCCGCTTCCGGGAAACCCGGGATCAGGTTCTCGA
TCTTTTCAGGAAAAGTCCTCTTTACCCCCTGCTCCGGGTCATGTCCCTGGCCGATTTTCAACAAACGGCATGA

Upstream 100 bases:

>100_bases
CGTAGGCGAAGAAGGCCTCAGGATCATTCCCAATCTTCAGGCGGCGATCAGGTTCTGCCGTCAGAAGGCCATCCCTGTTG
TTTATGCCAACGATGCGCTG

Downstream 100 bases:

>100_bases
AGAGAAAAAAATCGGCAAACGCTCTCTATATTGGGAATGACTTGAAGGTGTATCATGTGGGAAAGATATTTGCTTTTCCA
AAACAATACCCCGGCTAAAA

Product: pyrazinamidase / nicotinamidase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MPDDFLFKSRAKPHGIRGKAGAQVISELKPENGDIIVPKPRFSAFFKTDLDRILGERGVDTVVIGGVATEVCVLSTAYDA
VCHNFTVIVLEDCCASRFRETRDQVLDLFRKSPLYPLLRVMSLADFQQTA

Sequences:

>Translated_130_residues
MPDDFLFKSRAKPHGIRGKAGAQVISELKPENGDIIVPKPRFSAFFKTDLDRILGERGVDTVVIGGVATEVCVLSTAYDA
VCHNFTVIVLEDCCASRFRETRDQVLDLFRKSPLYPLLRVMSLADFQQTA
>Mature_129_residues
PDDFLFKSRAKPHGIRGKAGAQVISELKPENGDIIVPKPRFSAFFKTDLDRILGERGVDTVVIGGVATEVCVLSTAYDAV
CHNFTVIVLEDCCASRFRETRDQVLDLFRKSPLYPLLRVMSLADFQQTA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=78, Percent_Identity=46.1538461538462, Blast_Score=75, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 14462; Mature: 14331

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDDFLFKSRAKPHGIRGKAGAQVISELKPENGDIIVPKPRFSAFFKTDLDRILGERGVD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCC
TVVIGGVATEVCVLSTAYDAVCHNFTVIVLEDCCASRFRETRDQVLDLFRKSPLYPLLRV
EEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MSLADFQQTA
HHHHHHHHCC
>Mature Secondary Structure 
PDDFLFKSRAKPHGIRGKAGAQVISELKPENGDIIVPKPRFSAFFKTDLDRILGERGVD
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCC
TVVIGGVATEVCVLSTAYDAVCHNFTVIVLEDCCASRFRETRDQVLDLFRKSPLYPLLRV
EEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MSLADFQQTA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA