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The map label for this gene is prfC

Identifier: 85860354

GI number: 85860354

Start: 2533651

End: 2535240

Strand: Direct

Name: prfC

Synonym: SYN_00848

Alternate gene names: 85860354

Gene position: 2533651-2535240 (Clockwise)

Preceding gene: 85860348

Following gene: 85860356

Centisome position: 79.69

GC content: 57.8

Gene sequence:

>1590_bases
ATGTACCAGAAAGAAATCGAACAGCGCCGGACCTTCGGCATCATCAGCCATCCGGACGCCGGAAAAACCACCTTGACGGA
AAAGCTGCTCCTCTTTGGCGGGGCAATCCAGCTTGCCGGAACGGTCAAGGCCCGCAAGGCCACCCGCCACGCCACAAGCG
ACTGGATGGCCATCGAAAAGGAACGCGGCATTTCCGTAACCACCTCGGTGATGAAGTTTCATTACCGGGATTTTGAAATC
AACCTCCTCGACACCCCGGGGCATCAGGATTTCTCCGAGGACACCTACCGGGTGCTCACAGCGGTGGACAGCGCGGTCAT
GGTCATTGACGGGGCAAAGGGCGTGGAACCCCAGACGGAAAAACTCATGGAGATCTGCCTGCTGCGCAACACCCCGGTGA
TCACCTTCATCAACAAGCTGGACCGGGAGGTGTTGTCGCCCCTGGAACTGCTGGCCGATATCGAAAAGAAACTGCAGATC
GAATGCGTCCCCATGTCCTGGCCGATCGGAATGGGCAAGAGTTTCCGTGGGGTATACAACCTGTATCGCAGGGAACTGCA
ATTGTTTACGCCCGGCGCTTCAACCCGCGGCGGCGAGGTCTTCGTCATCCGCGATCTTGACGATCCGGAACTGGACAGGC
GGCTGGGTTCCCAGGCCGACGAACTGCGCGAAGACATCGCCCTGATGGAAGGAGCGGCTAATCCCTTTGTTTATGAGCAC
TTTCTGAAGGCGAATCAGACACCCGTTTTCTTCGGCAGCGCCATCAACAATTTCGGCGTCCGCGAGCTGCTGGACGCCGT
CGTGGAGCTGGCCCCCAGTCCCCGTCCCCGCCTGGCAACCACCCGAGAGGTTTTGCCCTCGGAAGAGCAGTTTTCCGGCT
TTGCCTTCAAGATTCAGGCGAATATGGACAGCGCCCACCGCGACCGCATCGCCTTCGTGCGGATCTGTTCCGGAAAATTC
ACCCGGGGGATGAAAGTCCGCCACAACCGGATCGGCAAAGACGTGACCCTGGCCAACGCCACGATCTTCATGGCCCAGGA
TCGCGCCTCGGTGGAAGAGGCCTATCCCGGAGACATCATCGGGATTCACAACCACGGAACCATCCGGATCGGGGACACCT
TCACCGAAGGAGAGGAACTGAGATTCACGGGAATCCCCAACTTCGCCCCGGAACACTTCTGCCGGGTCCGGCTGAAAAAT
CCCTTGAAAACCAAGCAGCTTCAGAAAGGACTTAACCAGCTATCTGAGGAAGGGATCGTTCAGGTCTTCCGTCCCCTGAC
CAGCAACGACTATATCCTGGGCGTGGTTGGCGTTCTGCAGTTTGACGTAACCATGGTGCGCCTTCTGAACGAATACGGCG
TCGAAGCGGATTACGAGTCGGTGGATTACGCCGCCGCCCGCTGGGTCACCTGCCCGGAGCGGGAAAAACTGGAAAATTTT
GAAAAGCAGCATCGGTCCAATCTGGCACTTGATTCCGAGGGGAATCTGATCTACCTTGCACTCAGCCAATGGCGCCTCTC
CAACACAATGGAGGAATGGCCCGATATCACGATGCACAAGACCATGGAGCACAGTCAGCGGCTCAATTGA

Upstream 100 bases:

>100_bases
ATCATCATTTTTATTATAGAAAGCCACGTTTATCATCGGGCCTCGAAAGGCCCGCTTTTTTTTACACTTCGACGAGAATT
GAAAGGATTTTGAAGTTAGG

Downstream 100 bases:

>100_bases
TGCGACGTTTGTTATAATTCCGGATCAAAGAGAACCGCAGGGCGGCGCGGCGCAACGATACAGGCTTGCAGAGGCGCCTG
TCGAACAGCCGATGGCGGAA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MYQKEIEQRRTFGIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKATRHATSDWMAIEKERGISVTTSVMKFHYRDFEI
NLLDTPGHQDFSEDTYRVLTAVDSAVMVIDGAKGVEPQTEKLMEICLLRNTPVITFINKLDREVLSPLELLADIEKKLQI
ECVPMSWPIGMGKSFRGVYNLYRRELQLFTPGASTRGGEVFVIRDLDDPELDRRLGSQADELREDIALMEGAANPFVYEH
FLKANQTPVFFGSAINNFGVRELLDAVVELAPSPRPRLATTREVLPSEEQFSGFAFKIQANMDSAHRDRIAFVRICSGKF
TRGMKVRHNRIGKDVTLANATIFMAQDRASVEEAYPGDIIGIHNHGTIRIGDTFTEGEELRFTGIPNFAPEHFCRVRLKN
PLKTKQLQKGLNQLSEEGIVQVFRPLTSNDYILGVVGVLQFDVTMVRLLNEYGVEADYESVDYAAARWVTCPEREKLENF
EKQHRSNLALDSEGNLIYLALSQWRLSNTMEEWPDITMHKTMEHSQRLN

Sequences:

>Translated_529_residues
MYQKEIEQRRTFGIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKATRHATSDWMAIEKERGISVTTSVMKFHYRDFEI
NLLDTPGHQDFSEDTYRVLTAVDSAVMVIDGAKGVEPQTEKLMEICLLRNTPVITFINKLDREVLSPLELLADIEKKLQI
ECVPMSWPIGMGKSFRGVYNLYRRELQLFTPGASTRGGEVFVIRDLDDPELDRRLGSQADELREDIALMEGAANPFVYEH
FLKANQTPVFFGSAINNFGVRELLDAVVELAPSPRPRLATTREVLPSEEQFSGFAFKIQANMDSAHRDRIAFVRICSGKF
TRGMKVRHNRIGKDVTLANATIFMAQDRASVEEAYPGDIIGIHNHGTIRIGDTFTEGEELRFTGIPNFAPEHFCRVRLKN
PLKTKQLQKGLNQLSEEGIVQVFRPLTSNDYILGVVGVLQFDVTMVRLLNEYGVEADYESVDYAAARWVTCPEREKLENF
EKQHRSNLALDSEGNLIYLALSQWRLSNTMEEWPDITMHKTMEHSQRLN
>Mature_529_residues
MYQKEIEQRRTFGIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKATRHATSDWMAIEKERGISVTTSVMKFHYRDFEI
NLLDTPGHQDFSEDTYRVLTAVDSAVMVIDGAKGVEPQTEKLMEICLLRNTPVITFINKLDREVLSPLELLADIEKKLQI
ECVPMSWPIGMGKSFRGVYNLYRRELQLFTPGASTRGGEVFVIRDLDDPELDRRLGSQADELREDIALMEGAANPFVYEH
FLKANQTPVFFGSAINNFGVRELLDAVVELAPSPRPRLATTREVLPSEEQFSGFAFKIQANMDSAHRDRIAFVRICSGKF
TRGMKVRHNRIGKDVTLANATIFMAQDRASVEEAYPGDIIGIHNHGTIRIGDTFTEGEELRFTGIPNFAPEHFCRVRLKN
PLKTKQLQKGLNQLSEEGIVQVFRPLTSNDYILGVVGVLQFDVTMVRLLNEYGVEADYESVDYAAARWVTCPEREKLENF
EKQHRSNLALDSEGNLIYLALSQWRLSNTMEEWPDITMHKTMEHSQRLN

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=497, Percent_Identity=28.7726358148893, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI19923640, Length=505, Percent_Identity=27.5247524752475, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI25306283, Length=464, Percent_Identity=28.2327586206897, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI25306287, Length=474, Percent_Identity=26.7932489451477, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=33.1395348837209, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=32.6530612244898, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=29.5302013422819, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI310132016, Length=122, Percent_Identity=34.4262295081967, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310110807, Length=122, Percent_Identity=34.4262295081967, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310123363, Length=122, Percent_Identity=34.4262295081967, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790835, Length=524, Percent_Identity=59.3511450381679, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=520, Percent_Identity=28.0769230769231, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI48994988, Length=184, Percent_Identity=33.1521739130435, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788922, Length=171, Percent_Identity=31.5789473684211, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=513, Percent_Identity=26.7056530214425, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=186, Percent_Identity=36.0215053763441, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=34.0909090909091, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=164, Percent_Identity=29.2682926829268, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=139, Percent_Identity=30.9352517985612, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=139, Percent_Identity=30.9352517985612, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=26.5560165975104, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322359, Length=551, Percent_Identity=25.7713248638838, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324707, Length=152, Percent_Identity=32.8947368421053, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=152, Percent_Identity=32.8947368421053, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=145, Percent_Identity=28.9655172413793, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=487, Percent_Identity=28.952772073922, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI221458488, Length=510, Percent_Identity=27.0588235294118, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI78706572, Length=176, Percent_Identity=34.0909090909091, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585709, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24585711, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24585713, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28574573, Length=154, Percent_Identity=32.4675324675325, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_SYNAS (Q2LWC5)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_462557.1
- ProteinModelPortal:   Q2LWC5
- SMR:   Q2LWC5
- STRING:   Q2LWC5
- GeneID:   3883305
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00848
- NMPDR:   fig|56780.10.peg.2503
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- PhylomeDB:   Q2LWC5
- ProtClustDB:   PRK00741
- BioCyc:   SACI56780:SYN_00848-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59991; Mature: 59991

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQKEIEQRRTFGIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKATRHATSDWMAIEK
CCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCHHEEHH
ERGISVTTSVMKFHYRDFEINLLDTPGHQDFSEDTYRVLTAVDSAVMVIDGAKGVEPQTE
CCCCCHHHHHHHHEECCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHH
KLMEICLLRNTPVITFINKLDREVLSPLELLADIEKKLQIECVPMSWPIGMGKSFRGVYN
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCHHHHHHH
LYRRELQLFTPGASTRGGEVFVIRDLDDPELDRRLGSQADELREDIALMEGAANPFVYEH
HHHHHHEEECCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHH
FLKANQTPVFFGSAINNFGVRELLDAVVELAPSPRPRLATTREVLPSEEQFSGFAFKIQA
HHHCCCCCEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHCCCEEEEEEE
NMDSAHRDRIAFVRICSGKFTRGMKVRHNRIGKDVTLANATIFMAQDRASVEEAYPGDII
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHCCCCEEEECEEEEEECCCHHHHHCCCCCEE
GIHNHGTIRIGDTFTEGEELRFTGIPNFAPEHFCRVRLKNPLKTKQLQKGLNQLSEEGIV
EEECCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QVFRPLTSNDYILGVVGVLQFDVTMVRLLNEYGVEADYESVDYAAARWVTCPEREKLENF
HHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHH
EKQHRSNLALDSEGNLIYLALSQWRLSNTMEEWPDITMHKTMEHSQRLN
HHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MYQKEIEQRRTFGIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKATRHATSDWMAIEK
CCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCHHEEHH
ERGISVTTSVMKFHYRDFEINLLDTPGHQDFSEDTYRVLTAVDSAVMVIDGAKGVEPQTE
CCCCCHHHHHHHHEECCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHH
KLMEICLLRNTPVITFINKLDREVLSPLELLADIEKKLQIECVPMSWPIGMGKSFRGVYN
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCHHHHHHH
LYRRELQLFTPGASTRGGEVFVIRDLDDPELDRRLGSQADELREDIALMEGAANPFVYEH
HHHHHHEEECCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHH
FLKANQTPVFFGSAINNFGVRELLDAVVELAPSPRPRLATTREVLPSEEQFSGFAFKIQA
HHHCCCCCEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHCCCEEEEEEE
NMDSAHRDRIAFVRICSGKFTRGMKVRHNRIGKDVTLANATIFMAQDRASVEEAYPGDII
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHCCCCEEEECEEEEEECCCHHHHHCCCCCEE
GIHNHGTIRIGDTFTEGEELRFTGIPNFAPEHFCRVRLKNPLKTKQLQKGLNQLSEEGIV
EEECCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QVFRPLTSNDYILGVVGVLQFDVTMVRLLNEYGVEADYESVDYAAARWVTCPEREKLENF
HHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHH
EKQHRSNLALDSEGNLIYLALSQWRLSNTMEEWPDITMHKTMEHSQRLN
HHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA