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The map label for this gene is pgaB [H]

Identifier: 85860345

GI number: 85860345

Start: 2521611

End: 2522510

Strand: Direct

Name: pgaB [H]

Synonym: SYN_00858

Alternate gene names: 85860345

Gene position: 2521611-2522510 (Clockwise)

Preceding gene: 85860343

Following gene: 85860346

Centisome position: 79.31

GC content: 53.78

Gene sequence:

>900_bases
ATGGAAGGAATGGGATACTCCCCTTTATCATTTTTACACCAAACGCAGCGCGGGCATCTATCAGCGCTACCTCGAGAAGC
TGCCGTCATGACGAAAAATCTTGATCGAAGAACTTTCCTGAAAGGAGCGGGCGCTCTGCTCGCTCTCCCGTTTATCGGAC
AGGCCTGTACATCACTGGCGCCGGTTCTCGACCAGGATTCCGTTATCACTGGGGCCGAAGGCGGCCTGCCGGTCCTGCTT
TATCATGATATTTCAGACTCCTTTTCCGACGCTTATACCGCAAGTGCCACTCAATTCGCCAGTCAGATGGAATGGCTTTA
CAGCAACGGCTATTCGGCAATTTCTCTCAGCCGGCTCAAGGATTCTCCACTTCCCGAAAAAGCCGTCGTCATCACCTTCG
ACGACGGATACGCGTCTTTCATGGATTTCGCTTTCCCCCTTCTGCAATCGTACCGGTTTAAAGCAACAATCAACATCATC
GGCAATGCTGTGGGATCTTATCTGCCTATGGCCGGCAACAGGCCCATGCTGAGCTGGGATGAGTACCGATATCTCACAGC
CGGGGGTATTGTTGACCTCGGCTGCCATACCTTTAAACTGCATGCCTTCCGCCACCGGGGTGTGCTGGATGTCCCGGAAA
AAATGCTTTACGACGATCTCAAGCTGTTCCAGGAAACCATGAAGCGGGAAATCGGCAGATCTTCCGGGATCATTGCCTGG
CCATACGGCTTCTACTCCCGGCGCGCAATGGAGATCGCCACACAGGCGGGATTCCACTGCATGCTTACGTCACGGCGGGG
CTTTTTCGGTCCGGCAAGCCCCCTGAACGAGATCCCCCGCATCTCTGTCGGCAGCGACATGAAATTCGAAACATTCAAGT
CAATACTGCACAGCGCATAG

Upstream 100 bases:

>100_bases
TCAAGCCGGAAAGTCAAAGCATGGCCGCCGTGCCAACCGGGCGGAACTTGAATGCCGCCGACATGCTGCCCTTGAGCGGC
TCGCCGTCACAGACATACAC

Downstream 100 bases:

>100_bases
TTTCCCAAAAAACGCCTCCCCGCCTTTACAGAAATTTATTTCATTTTTCAGGCGACATCCGGATTGCCTCTGCAGTTTTT
TCCGATCTCAGCCGCGATTG

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEGMGYSPLSFLHQTQRGHLSALPREAAVMTKNLDRRTFLKGAGALLALPFIGQACTSLAPVLDQDSVITGAEGGLPVLL
YHDISDSFSDAYTASATQFASQMEWLYSNGYSAISLSRLKDSPLPEKAVVITFDDGYASFMDFAFPLLQSYRFKATINII
GNAVGSYLPMAGNRPMLSWDEYRYLTAGGIVDLGCHTFKLHAFRHRGVLDVPEKMLYDDLKLFQETMKREIGRSSGIIAW
PYGFYSRRAMEIATQAGFHCMLTSRRGFFGPASPLNEIPRISVGSDMKFETFKSILHSA

Sequences:

>Translated_299_residues
MEGMGYSPLSFLHQTQRGHLSALPREAAVMTKNLDRRTFLKGAGALLALPFIGQACTSLAPVLDQDSVITGAEGGLPVLL
YHDISDSFSDAYTASATQFASQMEWLYSNGYSAISLSRLKDSPLPEKAVVITFDDGYASFMDFAFPLLQSYRFKATINII
GNAVGSYLPMAGNRPMLSWDEYRYLTAGGIVDLGCHTFKLHAFRHRGVLDVPEKMLYDDLKLFQETMKREIGRSSGIIAW
PYGFYSRRAMEIATQAGFHCMLTSRRGFFGPASPLNEIPRISVGSDMKFETFKSILHSA
>Mature_299_residues
MEGMGYSPLSFLHQTQRGHLSALPREAAVMTKNLDRRTFLKGAGALLALPFIGQACTSLAPVLDQDSVITGAEGGLPVLL
YHDISDSFSDAYTASATQFASQMEWLYSNGYSAISLSRLKDSPLPEKAVVITFDDGYASFMDFAFPLLQSYRFKATINII
GNAVGSYLPMAGNRPMLSWDEYRYLTAGGIVDLGCHTFKLHAFRHRGVLDVPEKMLYDDLKLFQETMKREIGRSSGIIAW
PYGFYSRRAMEIATQAGFHCMLTSRRGFFGPASPLNEIPRISVGSDMKFETFKSILHSA

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=286, Percent_Identity=24.8251748251748, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786322, Length=219, Percent_Identity=29.2237442922374, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 33119; Mature: 33119

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGMGYSPLSFLHQTQRGHLSALPREAAVMTKNLDRRTFLKGAGALLALPFIGQACTSLA
CCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHH
PVLDQDSVITGAEGGLPVLLYHDISDSFSDAYTASATQFASQMEWLYSNGYSAISLSRLK
HHCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHC
DSPLPEKAVVITFDDGYASFMDFAFPLLQSYRFKATINIIGNAVGSYLPMAGNRPMLSWD
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCCCCCCCHH
EYRYLTAGGIVDLGCHTFKLHAFRHRGVLDVPEKMLYDDLKLFQETMKREIGRSSGIIAW
CEEEEEECCEEECCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
PYGFYSRRAMEIATQAGFHCMLTSRRGFFGPASPLNEIPRISVGSDMKFETFKSILHSA
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEGMGYSPLSFLHQTQRGHLSALPREAAVMTKNLDRRTFLKGAGALLALPFIGQACTSLA
CCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHH
PVLDQDSVITGAEGGLPVLLYHDISDSFSDAYTASATQFASQMEWLYSNGYSAISLSRLK
HHCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHC
DSPLPEKAVVITFDDGYASFMDFAFPLLQSYRFKATINIIGNAVGSYLPMAGNRPMLSWD
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCCCCCCCHH
EYRYLTAGGIVDLGCHTFKLHAFRHRGVLDVPEKMLYDDLKLFQETMKREIGRSSGIIAW
CEEEEEECCEEECCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
PYGFYSRRAMEIATQAGFHCMLTSRRGFFGPASPLNEIPRISVGSDMKFETFKSILHSA
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]