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The map label for this gene is yfiS [H]

Identifier: 85860297

GI number: 85860297

Start: 2478377

End: 2479990

Strand: Direct

Name: yfiS [H]

Synonym: SYN_01220

Alternate gene names: 85860297

Gene position: 2478377-2479990 (Clockwise)

Preceding gene: 85860296

Following gene: 85860298

Centisome position: 77.95

GC content: 49.5

Gene sequence:

>1614_bases
ATGAACAGGAGAATACCAAGCCAGCAAATGAAATTCGTTATGCGGGCTTTGCGTTATCGGAATTACCGTCTTTTTTTCGT
TGGTCAGGGGATCTCTCTCATCGGCACCTGGATGCAGCAGCTCGCCTTGAGCTGGCTGGTGTACCGCCTGACCGAGTCTC
CCTTGTTTCTCGGAATTATAAGCTTTTCCAGCCAGATACCTTCTTTCATTTTGTCCCCTGTCGCGGGAGTCTATGCGGAC
CGATGGAATCGATACCATCTGGTTATCGCTACACAGTGTCTCGCCATGGTTCAGGCTTCCATTCTTGCAGTTCTTGCACT
GTCCGGGGCCATAACCGTCTGGCATCTTATTATTTTGAGTTTATTTCTGGGAATCGTCAATGCATTCGATGTTCCCATCA
GGCAATCCTTTGTTGTTGAAATGGTGGAACGTAAGGAAGATCTGGGAAATGCCATTGCGTTAAACTCCCTTCTTTTCAAC
GGCGCCCGACTCATCGGACCAACCCTGGCCGGAATGATCATCGCCTTCGCCGGGGAAGGAATCTGTTTTCTAATCAATGC
GATCAGCTTCCTCGCCGTTATCCCCGCCCTGCTGGCCATGAAAATTCCCACGAGGATCGTCCGATCTCGACGAGTTTATC
TGAAGGTAGGGCTGGAAGAAGGATATCATTACGTTCGCAACTCCATTCCTATTCGATACATCCTCATGCAGCTTTCCTTG
ATGAGCTTCATGGGAATGTCCTATGCGGTGCTTCTTCCTGTGTTCGCCCGGGATATCCTCGGGGGCGGTCCTCATAGCCT
GGGATTCCTCGTAGGAGCTGCCGGAGTAGGCGCCATGATAGGAGCGCTGTTTCTTGCATCGCGGGAATCAGTCATCGGAT
TGGGCAGGCTGATCGTCTTCTCCTCCGGACTCTTCGGAATCGGCGTGATCATTTTTTCCCTCTCGAAAGTTCTTCTGCTT
TCAATACTGATGATGTTTGGAATCGGCTTCGGCCTCATGGTACAAATGGCCTCCAGCAATACAATTCTTCAAACGATTGT
TGAAGAAGACAAGAGAGGGCGTATCATGAGCCTGTTTGCTGTTTCATTTATGGGCGTCGCTCCCTTCGGAAGCCTTTTTA
CAGGGTTGATGGCATCACATATCGGGGCGCCTGCAGCACTCGTAATTTGCGGGACCTGTTGCCTCCTGGGAACGGTTCTT
TTTTACAAAAAACTCCCGGATATCCGGAAGATCGTACGCCCAATCTACAAAGAAATGGGAATGTTCCCCCCCCTCGACGA
AGACTTGATGAATGCTGCAAAACCACTTTTGCATTTCACCAGGCGCTCCTGCCGTTATTTGCTTTTTCTCAACAGCTCCA
GCAACCTGTCCCGATACAGGTATCCCGCCAGCACAAGAATGCTGCTTACAGCGACAATCTCCAGCAACGTCAAATATTTA
TTGTTTCGAGCACAATTCCCGCTGACACTCAGAAGAATAGTTCCAAGCAGTCTTCCAATTGTTGAAATAATGGCCCACCA
TGGAAGGCCAACCAATGATCCTGCATCGTGGCCAAATAGCGGATCGATGAACAGACCAAAGATTTTAATCCCAGCTAATG
GTGCTCTGACATGA

Upstream 100 bases:

>100_bases
TCAAAAAGGAGGGGTCGTGATGAATGTTTCAAGAAGCAATAGTAAACAGGAAGGTGTCAGGATCTGTAAAGGCAATCTTT
CCTGACGCAAATTTTCAACA

Downstream 100 bases:

>100_bases
ACCCGTCCCGGACGTCCCACTCACTTGGAAATATGGGACGCACGACAAGGCTATGAAATGGAAATTTATCGGACAGCTCC
GACTTCAAGGGATACTTATT

Product: macrolide-efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MNRRIPSQQMKFVMRALRYRNYRLFFVGQGISLIGTWMQQLALSWLVYRLTESPLFLGIISFSSQIPSFILSPVAGVYAD
RWNRYHLVIATQCLAMVQASILAVLALSGAITVWHLIILSLFLGIVNAFDVPIRQSFVVEMVERKEDLGNAIALNSLLFN
GARLIGPTLAGMIIAFAGEGICFLINAISFLAVIPALLAMKIPTRIVRSRRVYLKVGLEEGYHYVRNSIPIRYILMQLSL
MSFMGMSYAVLLPVFARDILGGGPHSLGFLVGAAGVGAMIGALFLASRESVIGLGRLIVFSSGLFGIGVIIFSLSKVLLL
SILMMFGIGFGLMVQMASSNTILQTIVEEDKRGRIMSLFAVSFMGVAPFGSLFTGLMASHIGAPAALVICGTCCLLGTVL
FYKKLPDIRKIVRPIYKEMGMFPPLDEDLMNAAKPLLHFTRRSCRYLLFLNSSSNLSRYRYPASTRMLLTATISSNVKYL
LFRAQFPLTLRRIVPSSLPIVEIMAHHGRPTNDPASWPNSGSMNRPKILIPANGALT

Sequences:

>Translated_537_residues
MNRRIPSQQMKFVMRALRYRNYRLFFVGQGISLIGTWMQQLALSWLVYRLTESPLFLGIISFSSQIPSFILSPVAGVYAD
RWNRYHLVIATQCLAMVQASILAVLALSGAITVWHLIILSLFLGIVNAFDVPIRQSFVVEMVERKEDLGNAIALNSLLFN
GARLIGPTLAGMIIAFAGEGICFLINAISFLAVIPALLAMKIPTRIVRSRRVYLKVGLEEGYHYVRNSIPIRYILMQLSL
MSFMGMSYAVLLPVFARDILGGGPHSLGFLVGAAGVGAMIGALFLASRESVIGLGRLIVFSSGLFGIGVIIFSLSKVLLL
SILMMFGIGFGLMVQMASSNTILQTIVEEDKRGRIMSLFAVSFMGVAPFGSLFTGLMASHIGAPAALVICGTCCLLGTVL
FYKKLPDIRKIVRPIYKEMGMFPPLDEDLMNAAKPLLHFTRRSCRYLLFLNSSSNLSRYRYPASTRMLLTATISSNVKYL
LFRAQFPLTLRRIVPSSLPIVEIMAHHGRPTNDPASWPNSGSMNRPKILIPANGALT
>Mature_537_residues
MNRRIPSQQMKFVMRALRYRNYRLFFVGQGISLIGTWMQQLALSWLVYRLTESPLFLGIISFSSQIPSFILSPVAGVYAD
RWNRYHLVIATQCLAMVQASILAVLALSGAITVWHLIILSLFLGIVNAFDVPIRQSFVVEMVERKEDLGNAIALNSLLFN
GARLIGPTLAGMIIAFAGEGICFLINAISFLAVIPALLAMKIPTRIVRSRRVYLKVGLEEGYHYVRNSIPIRYILMQLSL
MSFMGMSYAVLLPVFARDILGGGPHSLGFLVGAAGVGAMIGALFLASRESVIGLGRLIVFSSGLFGIGVIIFSLSKVLLL
SILMMFGIGFGLMVQMASSNTILQTIVEEDKRGRIMSLFAVSFMGVAPFGSLFTGLMASHIGAPAALVICGTCCLLGTVL
FYKKLPDIRKIVRPIYKEMGMFPPLDEDLMNAAKPLLHFTRRSCRYLLFLNSSSNLSRYRYPASTRMLLTATISSNVKYL
LFRAQFPLTLRRIVPSSLPIVEIMAHHGRPTNDPASWPNSGSMNRPKILIPANGALT

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 59162; Mature: 59162

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRIPSQQMKFVMRALRYRNYRLFFVGQGISLIGTWMQQLALSWLVYRLTESPLFLGII
CCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHH
SFSSQIPSFILSPVAGVYADRWNRYHLVIATQCLAMVQASILAVLALSGAITVWHLIILS
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFLGIVNAFDVPIRQSFVVEMVERKEDLGNAIALNSLLFNGARLIGPTLAGMIIAFAGEG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
ICFLINAISFLAVIPALLAMKIPTRIVRSRRVYLKVGLEEGYHYVRNSIPIRYILMQLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHH
MSFMGMSYAVLLPVFARDILGGGPHSLGFLVGAAGVGAMIGALFLASRESVIGLGRLIVF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SSGLFGIGVIIFSLSKVLLLSILMMFGIGFGLMVQMASSNTILQTIVEEDKRGRIMSLFA
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VSFMGVAPFGSLFTGLMASHIGAPAALVICGTCCLLGTVLFYKKLPDIRKIVRPIYKEMG
HHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
MFPPLDEDLMNAAKPLLHFTRRSCRYLLFLNSSSNLSRYRYPASTRMLLTATISSNVKYL
CCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEECCCCCCEEEEEEECCCCEEE
LFRAQFPLTLRRIVPSSLPIVEIMAHHGRPTNDPASWPNSGSMNRPKILIPANGALT
EEEECCCHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MNRRIPSQQMKFVMRALRYRNYRLFFVGQGISLIGTWMQQLALSWLVYRLTESPLFLGII
CCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHH
SFSSQIPSFILSPVAGVYADRWNRYHLVIATQCLAMVQASILAVLALSGAITVWHLIILS
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFLGIVNAFDVPIRQSFVVEMVERKEDLGNAIALNSLLFNGARLIGPTLAGMIIAFAGEG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
ICFLINAISFLAVIPALLAMKIPTRIVRSRRVYLKVGLEEGYHYVRNSIPIRYILMQLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHH
MSFMGMSYAVLLPVFARDILGGGPHSLGFLVGAAGVGAMIGALFLASRESVIGLGRLIVF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SSGLFGIGVIIFSLSKVLLLSILMMFGIGFGLMVQMASSNTILQTIVEEDKRGRIMSLFA
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VSFMGVAPFGSLFTGLMASHIGAPAALVICGTCCLLGTVLFYKKLPDIRKIVRPIYKEMG
HHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
MFPPLDEDLMNAAKPLLHFTRRSCRYLLFLNSSSNLSRYRYPASTRMLLTATISSNVKYL
CCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEECCCCCCEEEEEEECCCCEEE
LFRAQFPLTLRRIVPSSLPIVEIMAHHGRPTNDPASWPNSGSMNRPKILIPANGALT
EEEECCCHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]