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The map label for this gene is hypB [H]

Identifier: 85860207

GI number: 85860207

Start: 2404721

End: 2405380

Strand: Direct

Name: hypB [H]

Synonym: SYN_00599

Alternate gene names: 85860207

Gene position: 2404721-2405380 (Clockwise)

Preceding gene: 85860206

Following gene: 85860208

Centisome position: 75.64

GC content: 54.39

Gene sequence:

>660_bases
ATGAAAGTAACCGTCGTCAAGAATGTCCTCGATGCCAATGAGCGGATCGCCAATGACAACCGCGGGCTCTTCGACAGGAA
AAAAATCTACGTCATCAACCTCATGAGCTCGCCCGGTGCGGGAAAGACATCTCTCGTGGAAAAGACGATTCTGGCACTCA
AGGACAGGTACCGGATCGCCGTCATCGAAGGGGACATTCAGGACACCTGCGACGCCGACCGGGTATCGGCTCTGGGGATT
CCCGCCGTCCAGATCAATACGGGAGGAGCCTGTCACATTGACGGCAACATGATCCGGGACGCCCTCCCCGCCCTCAACCT
GGATGAGATCGACCTGCTCATCACCGAGAATGTGGGCAATCTTGTCTGTCCAGCAGAGTTCAAGATCGGGGAAAACGCCA
AGGTGATGATTCTGAGCACCCCCGAAGGAGCGGACAAGCCTGCGAAATATCCCCTCATGTTTCAGGAATCCTCTGTGTTG
ATCCTCAACAAAATGGATCTTCTCCCCTACGTGGATTTCGACCTGGAAAGGGCAAAGCGCACCGCCCTGTCCCTGAATCG
GGATTTGAAGATTTTTGAAGTCTCCTGCAGGAATGACGAAGGCCTGGAAGGCTGGTATTCATGGCTTGCCGGACAGATCG
AGGCCTTTCGCAATTCATGA

Upstream 100 bases:

>100_bases
TGGTGTCCAGCGAGGGGACCTGCGCGGCATACCTGAAATATGAAATCCATAACCGAAAATCGTAACGGAAATTCCGTTAC
GGAAATAAAGGAGATGCTCC

Downstream 100 bases:

>100_bases
GCAGAAAGATAAGGCGCGTCCGCTGCCTTTTCACCGGCATCGTCCAGGGAGTGGGATTCCGCCCTTATATTTATAGAATG
GCGACCAACTACAGTCTCGG

Product: Ni2+ insertion GTPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKVTVVKNVLDANERIANDNRGLFDRKKIYVINLMSSPGAGKTSLVEKTILALKDRYRIAVIEGDIQDTCDADRVSALGI
PAVQINTGGACHIDGNMIRDALPALNLDEIDLLITENVGNLVCPAEFKIGENAKVMILSTPEGADKPAKYPLMFQESSVL
ILNKMDLLPYVDFDLERAKRTALSLNRDLKIFEVSCRNDEGLEGWYSWLAGQIEAFRNS

Sequences:

>Translated_219_residues
MKVTVVKNVLDANERIANDNRGLFDRKKIYVINLMSSPGAGKTSLVEKTILALKDRYRIAVIEGDIQDTCDADRVSALGI
PAVQINTGGACHIDGNMIRDALPALNLDEIDLLITENVGNLVCPAEFKIGENAKVMILSTPEGADKPAKYPLMFQESSVL
ILNKMDLLPYVDFDLERAKRTALSLNRDLKIFEVSCRNDEGLEGWYSWLAGQIEAFRNS
>Mature_219_residues
MKVTVVKNVLDANERIANDNRGLFDRKKIYVINLMSSPGAGKTSLVEKTILALKDRYRIAVIEGDIQDTCDADRVSALGI
PAVQINTGGACHIDGNMIRDALPALNLDEIDLLITENVGNLVCPAEFKIGENAKVMILSTPEGADKPAKYPLMFQESSVL
ILNKMDLLPYVDFDLERAKRTALSLNRDLKIFEVSCRNDEGLEGWYSWLAGQIEAFRNS

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=205, Percent_Identity=50.7317073170732, Blast_Score=217, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 24244; Mature: 24244

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTVVKNVLDANERIANDNRGLFDRKKIYVINLMSSPGAGKTSLVEKTILALKDRYRIA
CEEEEEHHHHCCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEE
VIEGDIQDTCDADRVSALGIPAVQINTGGACHIDGNMIRDALPALNLDEIDLLITENVGN
EEECCCCCCCCCCHHHHCCCCEEEEECCCEEEECCHHHHHHHCCCCCCCEEEEEECCCCC
LVCPAEFKIGENAKVMILSTPEGADKPAKYPLMFQESSVLILNKMDLLPYVDFDLERAKR
EECCCEEEECCCCEEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCHHHHHH
TALSLNRDLKIFEVSCRNDEGLEGWYSWLAGQIEAFRNS
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVTVVKNVLDANERIANDNRGLFDRKKIYVINLMSSPGAGKTSLVEKTILALKDRYRIA
CEEEEEHHHHCCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEE
VIEGDIQDTCDADRVSALGIPAVQINTGGACHIDGNMIRDALPALNLDEIDLLITENVGN
EEECCCCCCCCCCHHHHCCCCEEEEECCCEEEECCHHHHHHHCCCCCCCEEEEEECCCCC
LVCPAEFKIGENAKVMILSTPEGADKPAKYPLMFQESSVLILNKMDLLPYVDFDLERAKR
EECCCEEEECCCCEEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCHHHHHH
TALSLNRDLKIFEVSCRNDEGLEGWYSWLAGQIEAFRNS
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]