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The map label for this gene is ygcF [C]

Identifier: 85860085

GI number: 85860085

Start: 2291878

End: 2292525

Strand: Direct

Name: ygcF [C]

Synonym: SYN_01560

Alternate gene names: 85860085

Gene position: 2291878-2292525 (Clockwise)

Preceding gene: 85860084

Following gene: 85860086

Centisome position: 72.09

GC content: 52.47

Gene sequence:

>648_bases
GTGCCCCTGAAGGTCAACGAGATTTTCTACAGCATTCAGGGAGAATCCTCCTTTATCGGCATTCCCTGCGTTTTCGTCCG
GCTGACCGGCTGCAATCTCCGCTGCTCCTACTGCGACACCCGTTACGCCTACGAGGAAGGAACGCTGTGGGACATCCCCG
ACATTCTCGACCGGATATCCTCTTATCATTGTCCTCTCGTGGAAATCACGGGAGGGGAACCCCTGATCCAGAAGGAAACA
CCCCTTCTGATCCGCGATCTGCTGGACAGAGGATGCCATGTTCTGCTGGAAACCAATGGATCGAGACCCATTCGTACAAT
CGATGAACGCTGTGTACGGATTGTGGATATCAAATGCCCCTCCAGTGGGGAAAGCCGGAGCAACGATTACCGCAACCTGG
ATGATCTCACCGGACAGGATGAAATCAAGTTCGTCCTGGGCGAGAGAAAGGATTACGAGTTCGCTAAGGAAATCCTCATT
TCCCGGAATCTGGCAAACCGGATCTCCCATCCCCCCATCTTTTCTCCTGTCACCAATAGCCTTGATCCAAAAAAGCTGGT
CCGATGGATTCTGGAAGATCATCTTCCTGTCCGTTTTCAGCTCCAGCTTCACAAAATCCTGTGGGGAAACGATGTGCGGG
GTGTTTGA

Upstream 100 bases:

>100_bases
AACGAACTGAGTCGGAAACTGAATACGGAAAAGATCAGGGTCTCACGGGTCAGGGTTTCCGAAACGCCGACAAGCAAAGC
CTCTTACCGGGAGATATGAG

Downstream 100 bases:

>100_bases
AACTAGGGAAAAGACTCCAAGAGGCGGCAGGGAAAAGCCGGTGCAGTCGCATGTTTACATCATATCGAGGATACAGAGAC
GAAGCCGGTGCAGTCAGATG

Product: queuosine biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MPLKVNEIFYSIQGESSFIGIPCVFVRLTGCNLRCSYCDTRYAYEEGTLWDIPDILDRISSYHCPLVEITGGEPLIQKET
PLLIRDLLDRGCHVLLETNGSRPIRTIDERCVRIVDIKCPSSGESRSNDYRNLDDLTGQDEIKFVLGERKDYEFAKEILI
SRNLANRISHPPIFSPVTNSLDPKKLVRWILEDHLPVRFQLQLHKILWGNDVRGV

Sequences:

>Translated_215_residues
MPLKVNEIFYSIQGESSFIGIPCVFVRLTGCNLRCSYCDTRYAYEEGTLWDIPDILDRISSYHCPLVEITGGEPLIQKET
PLLIRDLLDRGCHVLLETNGSRPIRTIDERCVRIVDIKCPSSGESRSNDYRNLDDLTGQDEIKFVLGERKDYEFAKEILI
SRNLANRISHPPIFSPVTNSLDPKKLVRWILEDHLPVRFQLQLHKILWGNDVRGV
>Mature_214_residues
PLKVNEIFYSIQGESSFIGIPCVFVRLTGCNLRCSYCDTRYAYEEGTLWDIPDILDRISSYHCPLVEITGGEPLIQKETP
LLIRDLLDRGCHVLLETNGSRPIRTIDERCVRIVDIKCPSSGESRSNDYRNLDDLTGQDEIKFVLGERKDYEFAKEILIS
RNLANRISHPPIFSPVTNSLDPKKLVRWILEDHLPVRFQLQLHKILWGNDVRGV

Specific function: Unknown

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789139, Length=233, Percent_Identity=27.8969957081545, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 24730; Mature: 24599

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKVNEIFYSIQGESSFIGIPCVFVRLTGCNLRCSYCDTRYAYEEGTLWDIPDILDRIS
CCEEEEEEEEEECCCCCEEECEEEEEEEECCCEEECCCCCEEEECCCCEECHHHHHHHHH
SYHCPLVEITGGEPLIQKETPLLIRDLLDRGCHVLLETNGSRPIRTIDERCVRIVDIKCP
HCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHCEEEEEEECC
SSGESRSNDYRNLDDLTGQDEIKFVLGERKDYEFAKEILISRNLANRISHPPIFSPVTNS
CCCCCCCCCCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LDPKKLVRWILEDHLPVRFQLQLHKILWGNDVRGV
CCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
PLKVNEIFYSIQGESSFIGIPCVFVRLTGCNLRCSYCDTRYAYEEGTLWDIPDILDRIS
CEEEEEEEEEECCCCCEEECEEEEEEEECCCEEECCCCCEEEECCCCEECHHHHHHHHH
SYHCPLVEITGGEPLIQKETPLLIRDLLDRGCHVLLETNGSRPIRTIDERCVRIVDIKCP
HCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHCEEEEEEECC
SSGESRSNDYRNLDDLTGQDEIKFVLGERKDYEFAKEILISRNLANRISHPPIFSPVTNS
CCCCCCCCCCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LDPKKLVRWILEDHLPVRFQLQLHKILWGNDVRGV
CCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]