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The map label for this gene is glyS

Identifier: 85860061

GI number: 85860061

Start: 2273066

End: 2275138

Strand: Direct

Name: glyS

Synonym: SYN_01537

Alternate gene names: 85860061

Gene position: 2273066-2275138 (Clockwise)

Preceding gene: 85860060

Following gene: 85860065

Centisome position: 71.5

GC content: 53.84

Gene sequence:

>2073_bases
ATGAGCAATGAGTTGCTGCTTGAAATAGGAACGGAAGAAATCCCCGCGGCCTTTCTTCCCAAGGCACTTCAGGACATGAG
TTCGATGATCCGCAAAGCGCTTACCGAAGCGAGAATTCCCTTCGGACAGGTCCACACCTTCGGAACGCCCCGGCGACTCT
GCCTTGCCGTGGCCGATGTGGCTGAAAAGCAGGAAGATCAGGTCATTGAAAAACTGGGCCCCGCCAGAAGGGTTTCCTTT
GATGCGGACGGGAATCCCACCAAGGCCGCCCTGGGCTTCGCGAAAAGCCAGGGCGTCGACATTTCAGAGATCGGAACGAT
GCAGACCGATAAAGGGGAGTACATCTGCATCAGCAGACACATCAGCGGGAAAAGCACTGTTTCCCTGCTGTCGGAAATGC
TGTCCAGACTGATCACTTCTCTTTCCTTCAAGAAGTCCATGCGGTGGGGAAACCTGGATTTTCGGTTCGCCAGACCAATC
CACTGGATTCTTGCCCTTTACGGCGGCGAAGTCATCCCCTTCCGTATTGAAAACATCGAAAGCGGCGCGACCTCCCGGGG
GCACCGTTTCATGCATCCAGAGGCTTTTCCTGTGAGCAATCTGCAGGAGTATCTGGCCCGAACCAGAGAGCATTTTGTAA
TCGTAGCCCCCGAGGAAAGGAAGAGAATCATTCTTGAGGAAGCACGCAAAGCCGCTGCGGCCGTTTCCGGACGGGTGCTG
GAAAATGAAGACCTTCTGGAAACCGTCACGTATCTTGTGGAGTATCCCACCATCGTCTGTGGAAGTTTTGATCGAAAGTA
CCTAGAGCTTCCCAAGGAAGTCCTGATCACCTCCATGATGTCGCACCAGAAATATTTTCCTGTCGTGGACCAGGAAGGTC
GCCTGCTGCCCTTCTTTATTACGATCAACAATACCCTGGCCCGGGACCCCGCCGTTGTGACCCGGGGAAATGAAAAAGTC
ATCCGGGCGAGGCTGTCAGATGCCCAGTTTTTCTTTGAAGAAGATCAGAAGATCCGTCTCGATGACCGGGTGGAAGGACT
TCAACAGGTTGTTTTTCACACCCTGCTGGGAACGTCCTACGAAAAAGTTCAACGTTTCCGAAAGCTGGCCGGCTGGATCG
CCGACCGTATCGATCCGTCCCTGAAAAACAGAGTAAACCGCTCGGCTCTGCTGGCCAAAGCCGATCTGGATACTCAGATG
GTCGGAGAGTTTTCAGAGCTGCAGGGCATCATGGGGAGAGAATATGCCCTCCTTGCTGGCGAAGATCCGACTGTCGCCCG
GGCTATCTACGAACACTACCTCCCACTGACCGCCGGCGGCGATCTCCCCCAAACCCATGAAGGCGCCATTGTCAGCATTG
CCGACAAGATGGACAGCATCGCCGGATTCTTCGGCGTCAATCTGGTTCCCACAGGGACGGCGGATCCTTATGCCCTGCGC
CGGCAGGCCCTTGGTGTTATCAATATTATCCTTGATAAAAAGTATCCCCTGACGCTGGATGATCTGGTGGATGAGTGTAT
TTCGATTCTTGAGGAAAAGCTGAAGAGGCCGGCGGAAGAAACCCGGAAGGATGTTATAGAATTCTTCAGGGGAAGGCTTG
AAAACATGCTGATTTCCCAGGGCCACCCCCACGACGTGGTCAGCGCCGTTCTTGCCGCCGGTTTCGCCGATCTTGTCCAG
GTCATAAAAAAGATTGAGGCCATGGAATCCTTCAAAGCGCATCCCGCTTATGAACCACTGGCCATCGCCTTCAAGCGGGC
CGGCAACATCTTGAAGGAATTCAGAAACGGCCGAATCGATCCGGCTCTTTTCAGTGCCGCGGAAGAGAACCAGTTGTACT
CAACCCTTCTGGAGGCCCGCGCCAGGGTTGTCAAGGCCCTGGAGAAAGACGATTACCCTGCCGCACTTCTGGAACTTGCC
GCTCTCCGTCAGCCGATCGACCACTTTTTTGAATCGGTCATGGTCATGGTTGATGAAGAGAACATCCGCTTTAATCGGCT
TTCCCTTCTGGAAGCCCTGTTTTCAATCTTCCGCCGTATTGCAGATTTTTCACGAATCGTTACGGAATCCTGA

Upstream 100 bases:

>100_bases
CTGCTGAACAGATCGGAGGATTGACACTCGGATTCCTCAGGTAAAACAGGATCAACATTTCCGCTTTTTTGAAAGCTATT
CTTAAAGATGGGAGAATCAG

Downstream 100 bases:

>100_bases
CAATTATGGGGCGTTGAAGGAATCTCTGTTCTTCAACGCCCCACGGCATTCTACCTTTTGACAACCAGGACCGGTTGATC
CGCCTTGCGGATTACCGCTT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MSNELLLEIGTEEIPAAFLPKALQDMSSMIRKALTEARIPFGQVHTFGTPRRLCLAVADVAEKQEDQVIEKLGPARRVSF
DADGNPTKAALGFAKSQGVDISEIGTMQTDKGEYICISRHISGKSTVSLLSEMLSRLITSLSFKKSMRWGNLDFRFARPI
HWILALYGGEVIPFRIENIESGATSRGHRFMHPEAFPVSNLQEYLARTREHFVIVAPEERKRIILEEARKAAAAVSGRVL
ENEDLLETVTYLVEYPTIVCGSFDRKYLELPKEVLITSMMSHQKYFPVVDQEGRLLPFFITINNTLARDPAVVTRGNEKV
IRARLSDAQFFFEEDQKIRLDDRVEGLQQVVFHTLLGTSYEKVQRFRKLAGWIADRIDPSLKNRVNRSALLAKADLDTQM
VGEFSELQGIMGREYALLAGEDPTVARAIYEHYLPLTAGGDLPQTHEGAIVSIADKMDSIAGFFGVNLVPTGTADPYALR
RQALGVINIILDKKYPLTLDDLVDECISILEEKLKRPAEETRKDVIEFFRGRLENMLISQGHPHDVVSAVLAAGFADLVQ
VIKKIEAMESFKAHPAYEPLAIAFKRAGNILKEFRNGRIDPALFSAAEENQLYSTLLEARARVVKALEKDDYPAALLELA
ALRQPIDHFFESVMVMVDEENIRFNRLSLLEALFSIFRRIADFSRIVTES

Sequences:

>Translated_690_residues
MSNELLLEIGTEEIPAAFLPKALQDMSSMIRKALTEARIPFGQVHTFGTPRRLCLAVADVAEKQEDQVIEKLGPARRVSF
DADGNPTKAALGFAKSQGVDISEIGTMQTDKGEYICISRHISGKSTVSLLSEMLSRLITSLSFKKSMRWGNLDFRFARPI
HWILALYGGEVIPFRIENIESGATSRGHRFMHPEAFPVSNLQEYLARTREHFVIVAPEERKRIILEEARKAAAAVSGRVL
ENEDLLETVTYLVEYPTIVCGSFDRKYLELPKEVLITSMMSHQKYFPVVDQEGRLLPFFITINNTLARDPAVVTRGNEKV
IRARLSDAQFFFEEDQKIRLDDRVEGLQQVVFHTLLGTSYEKVQRFRKLAGWIADRIDPSLKNRVNRSALLAKADLDTQM
VGEFSELQGIMGREYALLAGEDPTVARAIYEHYLPLTAGGDLPQTHEGAIVSIADKMDSIAGFFGVNLVPTGTADPYALR
RQALGVINIILDKKYPLTLDDLVDECISILEEKLKRPAEETRKDVIEFFRGRLENMLISQGHPHDVVSAVLAAGFADLVQ
VIKKIEAMESFKAHPAYEPLAIAFKRAGNILKEFRNGRIDPALFSAAEENQLYSTLLEARARVVKALEKDDYPAALLELA
ALRQPIDHFFESVMVMVDEENIRFNRLSLLEALFSIFRRIADFSRIVTES
>Mature_689_residues
SNELLLEIGTEEIPAAFLPKALQDMSSMIRKALTEARIPFGQVHTFGTPRRLCLAVADVAEKQEDQVIEKLGPARRVSFD
ADGNPTKAALGFAKSQGVDISEIGTMQTDKGEYICISRHISGKSTVSLLSEMLSRLITSLSFKKSMRWGNLDFRFARPIH
WILALYGGEVIPFRIENIESGATSRGHRFMHPEAFPVSNLQEYLARTREHFVIVAPEERKRIILEEARKAAAAVSGRVLE
NEDLLETVTYLVEYPTIVCGSFDRKYLELPKEVLITSMMSHQKYFPVVDQEGRLLPFFITINNTLARDPAVVTRGNEKVI
RARLSDAQFFFEEDQKIRLDDRVEGLQQVVFHTLLGTSYEKVQRFRKLAGWIADRIDPSLKNRVNRSALLAKADLDTQMV
GEFSELQGIMGREYALLAGEDPTVARAIYEHYLPLTAGGDLPQTHEGAIVSIADKMDSIAGFFGVNLVPTGTADPYALRR
QALGVINIILDKKYPLTLDDLVDECISILEEKLKRPAEETRKDVIEFFRGRLENMLISQGHPHDVVSAVLAAGFADLVQV
IKKIEAMESFKAHPAYEPLAIAFKRAGNILKEFRNGRIDPALFSAAEENQLYSTLLEARARVVKALEKDDYPAALLELAA
LRQPIDHFFESVMVMVDEENIRFNRLSLLEALFSIFRRIADFSRIVTES

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=684, Percent_Identity=40.9356725146199, Blast_Score=490, Evalue=1e-139,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_SYNAS (Q2LVI9)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_462263.1
- ProteinModelPortal:   Q2LVI9
- STRING:   Q2LVI9
- GeneID:   3884074
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01537
- NMPDR:   fig|56780.10.peg.2199
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   Q2LVI9
- ProtClustDB:   PRK01233
- BioCyc:   SACI56780:SYN_01537-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 77604; Mature: 77473

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNELLLEIGTEEIPAAFLPKALQDMSSMIRKALTEARIPFGQVHTFGTPRRLCLAVADV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHH
AEKQEDQVIEKLGPARRVSFDADGNPTKAALGFAKSQGVDISEIGTMQTDKGEYICISRH
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEC
ISGKSTVSLLSEMLSRLITSLSFKKSMRWGNLDFRFARPIHWILALYGGEVIPFRIENIE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCEEEEEECCCC
SGATSRGHRFMHPEAFPVSNLQEYLARTREHFVIVAPEERKRIILEEARKAAAAVSGRVL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCC
ENEDLLETVTYLVEYPTIVCGSFDRKYLELPKEVLITSMMSHQKYFPVVDQEGRLLPFFI
CCHHHHHHHHHHHHCCHHEECCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
TINNTLARDPAVVTRGNEKVIRARLSDAQFFFEEDQKIRLDDRVEGLQQVVFHTLLGTSY
EECCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCH
EKVQRFRKLAGWIADRIDPSLKNRVNRSALLAKADLDTQMVGEFSELQGIMGREYALLAG
HHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCCHHHHHCHHHHHHHCCCCEEEEEC
EDPTVARAIYEHYLPLTAGGDLPQTHEGAIVSIADKMDSIAGFFGVNLVPTGTADPYALR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCEEECCCCCHHHHH
RQALGVINIILDKKYPLTLDDLVDECISILEEKLKRPAEETRKDVIEFFRGRLENMLISQ
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
GHPHDVVSAVLAAGFADLVQVIKKIEAMESFKAHPAYEPLAIAFKRAGNILKEFRNGRID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PALFSAAEENQLYSTLLEARARVVKALEKDDYPAALLELAALRQPIDHFFESVMVMVDEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCC
NIRFNRLSLLEALFSIFRRIADFSRIVTES
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNELLLEIGTEEIPAAFLPKALQDMSSMIRKALTEARIPFGQVHTFGTPRRLCLAVADV
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHH
AEKQEDQVIEKLGPARRVSFDADGNPTKAALGFAKSQGVDISEIGTMQTDKGEYICISRH
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEC
ISGKSTVSLLSEMLSRLITSLSFKKSMRWGNLDFRFARPIHWILALYGGEVIPFRIENIE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCEEEEEECCCC
SGATSRGHRFMHPEAFPVSNLQEYLARTREHFVIVAPEERKRIILEEARKAAAAVSGRVL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCC
ENEDLLETVTYLVEYPTIVCGSFDRKYLELPKEVLITSMMSHQKYFPVVDQEGRLLPFFI
CCHHHHHHHHHHHHCCHHEECCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
TINNTLARDPAVVTRGNEKVIRARLSDAQFFFEEDQKIRLDDRVEGLQQVVFHTLLGTSY
EECCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCH
EKVQRFRKLAGWIADRIDPSLKNRVNRSALLAKADLDTQMVGEFSELQGIMGREYALLAG
HHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCCHHHHHCHHHHHHHCCCCEEEEEC
EDPTVARAIYEHYLPLTAGGDLPQTHEGAIVSIADKMDSIAGFFGVNLVPTGTADPYALR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCEEECCCCCHHHHH
RQALGVINIILDKKYPLTLDDLVDECISILEEKLKRPAEETRKDVIEFFRGRLENMLISQ
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
GHPHDVVSAVLAAGFADLVQVIKKIEAMESFKAHPAYEPLAIAFKRAGNILKEFRNGRID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PALFSAAEENQLYSTLLEARARVVKALEKDDYPAALLELAALRQPIDHFFESVMVMVDEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCC
NIRFNRLSLLEALFSIFRRIADFSRIVTES
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA