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The map label for this gene is nadE

Identifier: 85859763

GI number: 85859763

Start: 1997809

End: 1999884

Strand: Direct

Name: nadE

Synonym: SYN_01307

Alternate gene names: 85859763

Gene position: 1997809-1999884 (Clockwise)

Preceding gene: 85859762

Following gene: 85859764

Centisome position: 62.84

GC content: 52.12

Gene sequence:

>2076_bases
GTGAAGCCTATGCCTTCCGATCCTGTAAATTTCTTTAATATATACTCCCACGGCTTCATCAGGACGGCGATCTGCATTCC
GGATGTCCGCGTCGCCGATACGGAGTTCAACGCGGAAAAAACCATCTCGATGGCAAAGGAAGCGGCCGCCGGCAAGGCAG
TCTTCGCTCTGTTCCCGGAATTGAATCTCTCGGCTTACTCCAATGAAGATCTTTTTCATCAGGACGCTCTGCAGGAGAAT
GTTTATGACGCCCTCTTCAAGGTCGCCCGTGAGACGGCTTCCCTGAACCTGATTCTCATTGTCGGCGCTCCCATCCGGGT
GGACTGCCGCCTTTACAACTGCGGGGTAATCCTTTATCAGGGCCGTATCCTTGGCATCGCCGTCAAATCCTATCTTCCCA
ATTACCGGGAATTTTATGAAGCCAGACAGTTCAGCCCGGCAGACCAGGCGATCGCCACATCCATTGACCTCGGTACTCAG
AATGACATCCCTTTCGGGGCCAATCTGATCTTTGAAGTAAAGAACATCAAAAATTTCAAATTTTTCATTGAGATATGCGA
AGATGTGTGGGTTCCCATACCGCCGTCCAGCTTTGCGGCCATGGCTGGGGCAACGGTTATCGCCAATCTGTCGGCATCGA
ACATAACGGTAGGAAAGTCGGAATATCGTCACAGCCTTACGGCAAATCAGTCGGCCCGGTGCGTTTCGGCTTATCTTTAC
GCCGCCGCCGGTCCCGGCGAATCCACCACAGATCTCGCCTGGGACGGGCATGCCATGATTTATGAAAACGGCAACCTGCT
TTCTGAATCACAACGGTTCTCCCGCGTTCCTCAGATTATTTTTGCGGACGTCGATCTGGACCGGCTGGCTCAGGATCGAA
TGCGCCTGACGACCTTCGGGTTGAATGCCCGCACCCATAAGGATATCCTCTGCCGGTTTCGCAGGATCGCCTTCGATATT
GCTCCCGTATCAGGAAAGATTTTCCTGATACGGCAGTATCCCCGTTTTCCCTACATCCCGGCTGACCCCAGCAAACGGGA
CCAGCGTTGCTATGAGTCATACAATATCCAGGTTCAGGGATTGTCAAAGCGCCTCGAATCCACGGGCATAAAAAATGTCG
TGATCGGCATCTCCGGAGGGCTGGATTCAACCCATGCCCTCATCGTCGCCGCAAAGACGATGGATCTTCTCGGATATCCA
AGATCCTGCATTAAGGCCTACACCATGCCCGGTTTTGCGACAACGGACAAAACCTACACCAATTCTCTCCGGCTTATGAA
AGCCCTGGAAGTGGAAGCCAACGAAATCGACATCCGACCCAGTTGCATCCAGATGCTGAAAGATATCGGTCATCCGTTTG
CTGAAGGGAAGAAGCTTTACGACATCACTTTTGAAAATATTCAGGCGGGGGAACGGACCTCCCACCTTTTCCGGATCGCC
AATATGAGGAACGCCCTGGTGCTCGGCACGGGAGATTTGAGCGAACTGGCCCTCGGCTGGTGCACCTATGGCGTCGGCGA
TCACATGTCGCACTACAGCGTAAACGCGAGCGTACCCAAAACCCTCATTCAGCATCTGATTCGGTGGGTGGCGCAGTCGA
ATCAATTTTCTCCGGAAACTTCGGAAACTCTTATTGATATTCTGGAGACAGAAATCAGTCCCGAGCTCGTTCCCGGTGAA
GAAAATCAGCAGCCAGCCCAGAAAACGGAATTGATTATCGGTCCATACGAACTTCAGGACTTCAACAATTTCTACACGAC
GCGCTTCGGTTATCTGCCGACCAAAATCGCCTTTATAGCCTATTGTACCTGGAGGAATAAAAAAGAAGGCATATGGCCTG
ATCTCCCGGAAGAAAAAAAACATGAATACACAATCGGCGAGATCAAGCACTGGCTGCAGATCTATCTTCTGCGATTCTTC
AAGCTCAGCCAGTTCAAGCGCTCCTGCATACCCAACAGCCCGAAGGTGGGTTCCGGCGGCTCGCTTTCCCCAAGGGGTGA
TTACAGGGCCCCCAGTGACAGTGAAGCGACCGTCTGGCTCAAAAACGCCAAGCAGATTCCGGAAAGGGATGATTAA

Upstream 100 bases:

>100_bases
AACGTCTCAATGAAAATTCCAATCTCACCTGGTACAGCGTTGAAGCGGAAAACTTTGAAAGCATTCATAATCATAATGCC
TATGCCTACGTGGAAAAAGA

Downstream 100 bases:

>100_bases
AAAAGAAAAGGGTTTCAGAATACGCCATGTTTAGAAGGATGAAGGATAACGGAAATTACATACAGGGAGAAGCTTACCGG
GACTTTGAAGCTACGGAGCT

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MKPMPSDPVNFFNIYSHGFIRTAICIPDVRVADTEFNAEKTISMAKEAAAGKAVFALFPELNLSAYSNEDLFHQDALQEN
VYDALFKVARETASLNLILIVGAPIRVDCRLYNCGVILYQGRILGIAVKSYLPNYREFYEARQFSPADQAIATSIDLGTQ
NDIPFGANLIFEVKNIKNFKFFIEICEDVWVPIPPSSFAAMAGATVIANLSASNITVGKSEYRHSLTANQSARCVSAYLY
AAAGPGESTTDLAWDGHAMIYENGNLLSESQRFSRVPQIIFADVDLDRLAQDRMRLTTFGLNARTHKDILCRFRRIAFDI
APVSGKIFLIRQYPRFPYIPADPSKRDQRCYESYNIQVQGLSKRLESTGIKNVVIGISGGLDSTHALIVAAKTMDLLGYP
RSCIKAYTMPGFATTDKTYTNSLRLMKALEVEANEIDIRPSCIQMLKDIGHPFAEGKKLYDITFENIQAGERTSHLFRIA
NMRNALVLGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLIQHLIRWVAQSNQFSPETSETLIDILETEISPELVPGE
ENQQPAQKTELIIGPYELQDFNNFYTTRFGYLPTKIAFIAYCTWRNKKEGIWPDLPEEKKHEYTIGEIKHWLQIYLLRFF
KLSQFKRSCIPNSPKVGSGGSLSPRGDYRAPSDSEATVWLKNAKQIPERDD

Sequences:

>Translated_691_residues
MKPMPSDPVNFFNIYSHGFIRTAICIPDVRVADTEFNAEKTISMAKEAAAGKAVFALFPELNLSAYSNEDLFHQDALQEN
VYDALFKVARETASLNLILIVGAPIRVDCRLYNCGVILYQGRILGIAVKSYLPNYREFYEARQFSPADQAIATSIDLGTQ
NDIPFGANLIFEVKNIKNFKFFIEICEDVWVPIPPSSFAAMAGATVIANLSASNITVGKSEYRHSLTANQSARCVSAYLY
AAAGPGESTTDLAWDGHAMIYENGNLLSESQRFSRVPQIIFADVDLDRLAQDRMRLTTFGLNARTHKDILCRFRRIAFDI
APVSGKIFLIRQYPRFPYIPADPSKRDQRCYESYNIQVQGLSKRLESTGIKNVVIGISGGLDSTHALIVAAKTMDLLGYP
RSCIKAYTMPGFATTDKTYTNSLRLMKALEVEANEIDIRPSCIQMLKDIGHPFAEGKKLYDITFENIQAGERTSHLFRIA
NMRNALVLGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLIQHLIRWVAQSNQFSPETSETLIDILETEISPELVPGE
ENQQPAQKTELIIGPYELQDFNNFYTTRFGYLPTKIAFIAYCTWRNKKEGIWPDLPEEKKHEYTIGEIKHWLQIYLLRFF
KLSQFKRSCIPNSPKVGSGGSLSPRGDYRAPSDSEATVWLKNAKQIPERDD
>Mature_691_residues
MKPMPSDPVNFFNIYSHGFIRTAICIPDVRVADTEFNAEKTISMAKEAAAGKAVFALFPELNLSAYSNEDLFHQDALQEN
VYDALFKVARETASLNLILIVGAPIRVDCRLYNCGVILYQGRILGIAVKSYLPNYREFYEARQFSPADQAIATSIDLGTQ
NDIPFGANLIFEVKNIKNFKFFIEICEDVWVPIPPSSFAAMAGATVIANLSASNITVGKSEYRHSLTANQSARCVSAYLY
AAAGPGESTTDLAWDGHAMIYENGNLLSESQRFSRVPQIIFADVDLDRLAQDRMRLTTFGLNARTHKDILCRFRRIAFDI
APVSGKIFLIRQYPRFPYIPADPSKRDQRCYESYNIQVQGLSKRLESTGIKNVVIGISGGLDSTHALIVAAKTMDLLGYP
RSCIKAYTMPGFATTDKTYTNSLRLMKALEVEANEIDIRPSCIQMLKDIGHPFAEGKKLYDITFENIQAGERTSHLFRIA
NMRNALVLGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLIQHLIRWVAQSNQFSPETSETLIDILETEISPELVPGE
ENQQPAQKTELIIGPYELQDFNNFYTTRFGYLPTKIAFIAYCTWRNKKEGIWPDLPEEKKHEYTIGEIKHWLQIYLLRFF
KLSQFKRSCIPNSPKVGSGGSLSPRGDYRAPSDSEATVWLKNAKQIPERDD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=293, Percent_Identity=25.5972696245734, Blast_Score=90, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17538756, Length=276, Percent_Identity=28.2608695652174, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321865, Length=310, Percent_Identity=27.4193548387097, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641841, Length=293, Percent_Identity=25.5972696245734, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24641843, Length=293, Percent_Identity=25.5972696245734, Blast_Score=83, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 77599; Mature: 77599

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPMPSDPVNFFNIYSHGFIRTAICIPDVRVADTEFNAEKTISMAKEAAAGKAVFALFPE
CCCCCCCCCCHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCEEEEECCC
LNLSAYSNEDLFHQDALQENVYDALFKVARETASLNLILIVGAPIRVDCRLYNCGVILYQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEEEEECCEEEEE
GRILGIAVKSYLPNYREFYEARQFSPADQAIATSIDLGTQNDIPFGANLIFEVKNIKNFK
CEEEEEEHHHHCCCHHHHHHHHCCCCHHHHHHEEECCCCCCCCCCCCEEEEEECCCCCHH
FFIEICEDVWVPIPPSSFAAMAGATVIANLSASNITVGKSEYRHSLTANQSARCVSAYLY
HHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCCEEECHHHHHHHHCCCCCHHHHHHHHE
AAAGPGESTTDLAWDGHAMIYENGNLLSESQRFSRVPQIIFADVDLDRLAQDRMRLTTFG
EECCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHEEEEEC
LNARTHKDILCRFRRIAFDIAPVSGKIFLIRQYPRFPYIPADPSKRDQRCYESYNIQVQG
CCCCCHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCEEEEE
LSKRLESTGIKNVVIGISGGLDSTHALIVAAKTMDLLGYPRSCIKAYTMPGFATTDKTYT
HHHHHHHCCCCEEEEEEECCCCCCCEEEEEEHHHHHHCCCHHHHHHHCCCCCCCCCCHHH
NSLRLMKALEVEANEIDIRPSCIQMLKDIGHPFAEGKKLYDITFENIQAGERTSHLFRIA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
NMRNALVLGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLIQHLIRWVAQSNQFSPET
CCCCEEEEECCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCH
SETLIDILETEISPELVPGEENQQPAQKTELIIGPYELQDFNNFYTTRFGYLPTKIAFIA
HHHHHHHHHHCCCCCCCCCCCCCCCHHHCCEEECCHHHCCCCCHHHEECCCCHHHEEEEE
YCTWRNKKEGIWPDLPEEKKHEYTIGEIKHWLQIYLLRFFKLSQFKRSCIPNSPKVGSGG
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SLSPRGDYRAPSDSEATVWLKNAKQIPERDD
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKPMPSDPVNFFNIYSHGFIRTAICIPDVRVADTEFNAEKTISMAKEAAAGKAVFALFPE
CCCCCCCCCCHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCEEEEECCC
LNLSAYSNEDLFHQDALQENVYDALFKVARETASLNLILIVGAPIRVDCRLYNCGVILYQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEEEEECCEEEEE
GRILGIAVKSYLPNYREFYEARQFSPADQAIATSIDLGTQNDIPFGANLIFEVKNIKNFK
CEEEEEEHHHHCCCHHHHHHHHCCCCHHHHHHEEECCCCCCCCCCCCEEEEEECCCCCHH
FFIEICEDVWVPIPPSSFAAMAGATVIANLSASNITVGKSEYRHSLTANQSARCVSAYLY
HHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCCEEECHHHHHHHHCCCCCHHHHHHHHE
AAAGPGESTTDLAWDGHAMIYENGNLLSESQRFSRVPQIIFADVDLDRLAQDRMRLTTFG
EECCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHEEEEEC
LNARTHKDILCRFRRIAFDIAPVSGKIFLIRQYPRFPYIPADPSKRDQRCYESYNIQVQG
CCCCCHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCEEEEE
LSKRLESTGIKNVVIGISGGLDSTHALIVAAKTMDLLGYPRSCIKAYTMPGFATTDKTYT
HHHHHHHCCCCEEEEEEECCCCCCCEEEEEEHHHHHHCCCHHHHHHHCCCCCCCCCCHHH
NSLRLMKALEVEANEIDIRPSCIQMLKDIGHPFAEGKKLYDITFENIQAGERTSHLFRIA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
NMRNALVLGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLIQHLIRWVAQSNQFSPET
CCCCEEEEECCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCH
SETLIDILETEISPELVPGEENQQPAQKTELIIGPYELQDFNNFYTTRFGYLPTKIAFIA
HHHHHHHHHHCCCCCCCCCCCCCCCHHHCCEEECCHHHCCCCCHHHEECCCCHHHEEEEE
YCTWRNKKEGIWPDLPEEKKHEYTIGEIKHWLQIYLLRFFKLSQFKRSCIPNSPKVGSGG
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SLSPRGDYRAPSDSEATVWLKNAKQIPERDD
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]