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The map label for this gene is pth

Identifier: 85859732

GI number: 85859732

Start: 1961192

End: 1961782

Strand: Direct

Name: pth

Synonym: SYN_02769

Alternate gene names: 85859732

Gene position: 1961192-1961782 (Clockwise)

Preceding gene: 85859731

Following gene: 85859733

Centisome position: 61.69

GC content: 50.42

Gene sequence:

>591_bases
TTGAAGCTGATTGTGGGACTCGGGAACCCCGGCGCCCGGTACCGATTCACCCGGCATAATTTTGGATTTTTTGTGCTGGA
TCAGCTGGCGGAAGATCAGGATATCATGATGTCGCAGACAGGGTTTGACGCCCTGTGGGGGAAGGGAGTAATTGCCGGAC
AGAGCGTTCTGCTTGCAAAGCCGCAGACGTTCATGAATCTGAGTGGAAAATCCGTCAGGAAACTGGCGGATTTCTTTAAG
ATTGTCGTTGAAGATGTTTTAGTCGTTCATGATGATCTGGATCTGCCTTTCGGAGTCATCCGACTCAAGGCAGGGGGGGG
TCAGGGAGGCCATAAGGGGTTGATATCCATTTGTGACTCTCTTGGCGGGCCGGAATTCCAACGGGTCAGACTGGGAATTG
GAAAGCCGGCTCAGAGATCGGCGGTTGAGCGATACGTTCTTGAACCATTCACTGAAAGTGAATTGCGCATTTTGCCCAGG
ATCATCGTAACAGCACGCAGCGCGATTCTTGAGGTGATTTCTTCAGGAATCAAAACGGCTATGAACAGCTACAACGGTAT
GGTTATCAACGACTTAATACAGGAGGTTTGA

Upstream 100 bases:

>100_bases
TTCACGGCGTGAACAAAGCTGAAGAAACGGAAGAAGCTTCCTCATAAAGGCATTTCGTCATCACGGAAAGTCCTTATCAT
TTCATTATTGAGAGGTTCTT

Downstream 100 bases:

>100_bases
TGATGTTTAAAGGAAAACGCAATTTTTTGTCTTTCTTCATCATTAGCGCCATACTTTTCTTGCTGGCGCCAATGGCCTTC
GCAGGGGAAGCCGATATCGA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKLIVGLGNPGARYRFTRHNFGFFVLDQLAEDQDIMMSQTGFDALWGKGVIAGQSVLLAKPQTFMNLSGKSVRKLADFFK
IVVEDVLVVHDDLDLPFGVIRLKAGGGQGGHKGLISICDSLGGPEFQRVRLGIGKPAQRSAVERYVLEPFTESELRILPR
IIVTARSAILEVISSGIKTAMNSYNGMVINDLIQEV

Sequences:

>Translated_196_residues
MKLIVGLGNPGARYRFTRHNFGFFVLDQLAEDQDIMMSQTGFDALWGKGVIAGQSVLLAKPQTFMNLSGKSVRKLADFFK
IVVEDVLVVHDDLDLPFGVIRLKAGGGQGGHKGLISICDSLGGPEFQRVRLGIGKPAQRSAVERYVLEPFTESELRILPR
IIVTARSAILEVISSGIKTAMNSYNGMVINDLIQEV
>Mature_196_residues
MKLIVGLGNPGARYRFTRHNFGFFVLDQLAEDQDIMMSQTGFDALWGKGVIAGQSVLLAKPQTFMNLSGKSVRKLADFFK
IVVEDVLVVHDDLDLPFGVIRLKAGGGQGGHKGLISICDSLGGPEFQRVRLGIGKPAQRSAVERYVLEPFTESELRILPR
IIVTARSAILEVISSGIKTAMNSYNGMVINDLIQEV

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=174, Percent_Identity=36.7816091954023, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI1787455, Length=184, Percent_Identity=42.3913043478261, Blast_Score=116, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321983, Length=169, Percent_Identity=30.1775147928994, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_SYNAS (Q2LUK5)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461934.1
- ProteinModelPortal:   Q2LUK5
- SMR:   Q2LUK5
- STRING:   Q2LUK5
- GeneID:   3883608
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02769
- NMPDR:   fig|56780.10.peg.1878
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- PhylomeDB:   Q2LUK5
- ProtClustDB:   PRK05426
- BioCyc:   SACI56780:SYN_02769-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21447; Mature: 21447

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVGLGNPGARYRFTRHNFGFFVLDQLAEDQDIMMSQTGFDALWGKGVIAGQSVLLAK
CEEEEECCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHCCCCHHHHCCCEECCCEEEEEC
PQTFMNLSGKSVRKLADFFKIVVEDVLVVHDDLDLPFGVIRLKAGGGQGGHKGLISICDS
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
LGGPEFQRVRLGIGKPAQRSAVERYVLEPFTESELRILPRIIVTARSAILEVISSGIKTA
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNSYNGMVINDLIQEV
HHCCCCCHHHHHHHHC
>Mature Secondary Structure
MKLIVGLGNPGARYRFTRHNFGFFVLDQLAEDQDIMMSQTGFDALWGKGVIAGQSVLLAK
CEEEEECCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHCCCCHHHHCCCEECCCEEEEEC
PQTFMNLSGKSVRKLADFFKIVVEDVLVVHDDLDLPFGVIRLKAGGGQGGHKGLISICDS
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
LGGPEFQRVRLGIGKPAQRSAVERYVLEPFTESELRILPRIIVTARSAILEVISSGIKTA
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNSYNGMVINDLIQEV
HHCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA