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The map label for this gene is holB [H]

Identifier: 85859639

GI number: 85859639

Start: 1866482

End: 1867471

Strand: Direct

Name: holB [H]

Synonym: SYN_02985

Alternate gene names: 85859639

Gene position: 1866482-1867471 (Clockwise)

Preceding gene: 85859638

Following gene: 85859640

Centisome position: 58.71

GC content: 51.01

Gene sequence:

>990_bases
ATGGAGGCATTTCTCATGTCATTCAAGGACATCCTCGGTCACGCGAAACCCATAGGCATTCTTCAGAATGCCATACAACA
TAATCGTATGGCCCATGCCTATCTGTTCCATGGAATGGCAGGCGTAGGGAAACTCACCCTTGCCCTTAATTTTGCCAAAG
CGCTGAACTGCCTGCAGGGGGCCTCCGATGCCTGTGACTGCTGCATTTCCTGCATAAAGACCGATCATCGCAATCACCCG
GATATCGAAATGATTGAAGCTGAGGGTCAGTACATCCGGATCGATGCCGTAAAAGACCTTCAGGAGCGGATGAAATTCAA
ACCTATGGAGGGGAAGAAGCGCGTCGCCATCCTCGTGGACGCCGACCGCCTGAATAATGCCGCAGCCAACGCTCTGCTGA
AAACCCTCGAGGAACCATCACCGGCCAATATACTGATTCTGGTAACCGACAGACCCTACCAGCTTCCCATAACGATTCTT
TCACGATGTCAACAGATCCGCTTCAATCCTCTTGCTCAGGAAATAGTAACCACTCTGCTCCACGAGCGTTTTTCCGTCGA
AAACACCCAGGCCGGACTTCTGGCTGCCGCCTCCGGGGGAAGCATAGGCAAAGCCCTGGATCTGAACCGCGAAGCTTATC
TCACTTTAAAGAACGAAGTCCTGAACTTCATCGCCGGAATTTCTGCGGATTCATCCCTTCATCGATTAACTTTTCTTCGT
TTTTTAAGCGACGACAAGAGAGACATTATGGACAAACTGGACATCCTGAGAACCTTTTTCCGTGATGTCCTGATCCTTAG
GGAGACGGGAAGGGCAGAAGGACTCATGCACCAGGACCGTGTTGACCAGATTACCGCCTTTGCAGGGAAACGCACCACCG
GGGACATCCTGACTGCCATCCGGGCCATTGATTCGGCGGAACGTCGGCTGATACAGAATACGAATAAATCGTTGACTTTA
GAGGTCATGATGTTTAAACTCGCGCTCTGA

Upstream 100 bases:

>100_bases
TAGCTGGGAATCTCCGGTTTTTTTCAGGCCACTATTTTCATCTGCGCTTCATTTCCGGCCGCATCGCTGCATCAGGCAGC
AAAGAAACCCGCTTCAGATA

Downstream 100 bases:

>100_bases
TCAACAGATGACCGCAAAACGGCTTTATTCCAAACCTATATCAAAGGGCGCTCTTCGTCGGCTTCGCGATCGGTTTTCAT
ATATGCGTCGGGGTTCGCGT

Product: DNA polymerase III subunit gamma

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MEAFLMSFKDILGHAKPIGILQNAIQHNRMAHAYLFHGMAGVGKLTLALNFAKALNCLQGASDACDCCISCIKTDHRNHP
DIEMIEAEGQYIRIDAVKDLQERMKFKPMEGKKRVAILVDADRLNNAAANALLKTLEEPSPANILILVTDRPYQLPITIL
SRCQQIRFNPLAQEIVTTLLHERFSVENTQAGLLAAASGGSIGKALDLNREAYLTLKNEVLNFIAGISADSSLHRLTFLR
FLSDDKRDIMDKLDILRTFFRDVLILRETGRAEGLMHQDRVDQITAFAGKRTTGDILTAIRAIDSAERRLIQNTNKSLTL
EVMMFKLAL

Sequences:

>Translated_329_residues
MEAFLMSFKDILGHAKPIGILQNAIQHNRMAHAYLFHGMAGVGKLTLALNFAKALNCLQGASDACDCCISCIKTDHRNHP
DIEMIEAEGQYIRIDAVKDLQERMKFKPMEGKKRVAILVDADRLNNAAANALLKTLEEPSPANILILVTDRPYQLPITIL
SRCQQIRFNPLAQEIVTTLLHERFSVENTQAGLLAAASGGSIGKALDLNREAYLTLKNEVLNFIAGISADSSLHRLTFLR
FLSDDKRDIMDKLDILRTFFRDVLILRETGRAEGLMHQDRVDQITAFAGKRTTGDILTAIRAIDSAERRLIQNTNKSLTL
EVMMFKLAL
>Mature_329_residues
MEAFLMSFKDILGHAKPIGILQNAIQHNRMAHAYLFHGMAGVGKLTLALNFAKALNCLQGASDACDCCISCIKTDHRNHP
DIEMIEAEGQYIRIDAVKDLQERMKFKPMEGKKRVAILVDADRLNNAAANALLKTLEEPSPANILILVTDRPYQLPITIL
SRCQQIRFNPLAQEIVTTLLHERFSVENTQAGLLAAASGGSIGKALDLNREAYLTLKNEVLNFIAGISADSSLHRLTFLR
FLSDDKRDIMDKLDILRTFFRDVLILRETGRAEGLMHQDRVDQITAFAGKRTTGDILTAIRAIDSAERRLIQNTNKSLTL
EVMMFKLAL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=212, Percent_Identity=34.4339622641509, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787341, Length=238, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36728; Mature: 36728

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFLMSFKDILGHAKPIGILQNAIQHNRMAHAYLFHGMAGVGKLTLALNFAKALNCLQG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
ASDACDCCISCIKTDHRNHPDIEMIEAEGQYIRIDAVKDLQERMKFKPMEGKKRVAILVD
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHCCCCCCCCCEEEEEEE
ADRLNNAAANALLKTLEEPSPANILILVTDRPYQLPITILSRCQQIRFNPLAQEIVTTLL
HHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
HERFSVENTQAGLLAAASGGSIGKALDLNREAYLTLKNEVLNFIAGISADSSLHRLTFLR
HHHHCCCCCCCCEEEECCCCCCCCCEECCCHHEEEEHHHHHHHHHCCCCCCHHHHHHHHH
FLSDDKRDIMDKLDILRTFFRDVLILRETGRAEGLMHQDRVDQITAFAGKRTTGDILTAI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
RAIDSAERRLIQNTNKSLTLEVMMFKLAL
HHHHHHHHHHHHCCCCCEEHHHHHHHHCC
>Mature Secondary Structure
MEAFLMSFKDILGHAKPIGILQNAIQHNRMAHAYLFHGMAGVGKLTLALNFAKALNCLQG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
ASDACDCCISCIKTDHRNHPDIEMIEAEGQYIRIDAVKDLQERMKFKPMEGKKRVAILVD
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHCCCCCCCCCEEEEEEE
ADRLNNAAANALLKTLEEPSPANILILVTDRPYQLPITILSRCQQIRFNPLAQEIVTTLL
HHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
HERFSVENTQAGLLAAASGGSIGKALDLNREAYLTLKNEVLNFIAGISADSSLHRLTFLR
HHHHCCCCCCCCEEEECCCCCCCCCEECCCHHEEEEHHHHHHHHHCCCCCCHHHHHHHHH
FLSDDKRDIMDKLDILRTFFRDVLILRETGRAEGLMHQDRVDQITAFAGKRTTGDILTAI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
RAIDSAERRLIQNTNKSLTLEVMMFKLAL
HHHHHHHHHHHHCCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]