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The map label for this gene is spoT [H]

Identifier: 85859558

GI number: 85859558

Start: 1780697

End: 1782202

Strand: Direct

Name: spoT [H]

Synonym: SYN_01901

Alternate gene names: 85859558

Gene position: 1780697-1782202 (Clockwise)

Preceding gene: 85859555

Following gene: 85859559

Centisome position: 56.01

GC content: 50.4

Gene sequence:

>1506_bases
ATGGGACGAGATACTCAGGTATTGGCTTGTGGCAGCCTATTTATTGAAAAAATCGAAAATACCTACACGGCAAAAGATGC
CAACCTCTTAAAAAAGGCATACGCCTTTTCAAGACAGCGAGAATCCGACTGCGATTCATCATCTTTCAAGGCCGCTGAAT
TGCTCATTGAGCAGGGGGCGGATGGCGAAACTGTTGCGTGTGCCCTGGTAGCCCCCCATTTTTGGCAAGGCCGCGTTAAA
CCAGAGGAAATTCGGGAACACGTCAGTCAAGATGTAGCCGATACACTCGCATATCTCAAACAACCCTTCAGCCTCCGTAT
CGATACCGAAATCCATCGGCGCAAGGATATTAATGCTCTCCTAGTGTCCATGTCCGAGACGCCCCGCGCGGCCATTCTTC
TTATCGTTTTCCGTCTAATAGAATTGGAAACGACGCTTGAATCCCAAGGAAAGAATCCATGCCATATGGCGCAGGAAACG
CTTCATTTCTATGTGCCAATCGCGGATCGGTTAAGCCTTGGCGAGATGCGCCGCCGTCTTGAAGATGTTTGCTTTCGCAT
TTTGCATCCCTTTCAGTATGAAAAACTGAAGCAGGACGTGACTCCGATTCAGTCCGAAGATGAGAAGTGTCTGGAGATAC
TGATTGAAGGCGTCAAACGTCTGTTGGACAAGAACCGCATTCATGCGGAAGTTCACGGTCGCGCAAAGAGCCTTTATAGC
ATCCACCTGAAGATGACCCTAAAGGGAACGGCGCTGGAGGACATCATGGATCGCATCGGACTGCGAATCATCGTCTCGTC
CGTGCCCGAATGCTATGCTGTACTCGGACTGCTGCATACCCATTTCAAACCGATTCCCGGAACCTTTGACGATTACATCG
GACTTCCCAAGGATAATGGCTACCAGTCTCTGCACACCTGTGTCTACCCAATGCGAGAGGTCACCCATAAACCGATTGAG
TTCCAGGTGCGGACCGAACTTATGCACATGGAAGCTGAGCACGGCTCGGCTGCGCATTGGCTCTATAAAAGTGCTGTCGC
TATGGGAAAAGACTCCCTCAAAAACCAATGGTTGAAGGGGCTCGTGCGCCGGCATGACCAGGCAAAGAGCACCGATGCGT
TTATTGAACTTTTACGCCGTCAGGTTTGCGAGGATCACATGGTGGTTTTCGGCAAGGGCGGCAGAATCACCCGCTTGCCC
GACAAAGCCACCGTTCTCGAATATCTGGATGCCGCCAATTTCTTTGCTTCACGGAGCAGTGTCGTAAAGGTGAATGGAAA
ATTAGCCAGCTTAGATCAAACCCTCCGGGATGGAGATTCCATTGAGATCGTTGACTGCGAAGATTCTGCCAGTCCAGGTA
CAGTTGTTGATGACATGGGGGACATAATCGGACAGCATAAAGCGTCACCCATGAACACGGAAGGTTGCAAGGTCTGGGCC
ACAGCGCAAGGATCTATTTTGAGACATAAAGAGGAGAACAGCCATGCCTCAACAGAAGGTCCATGA

Upstream 100 bases:

>100_bases
AATGATACTGGGGGGTGATATGAGTTGATACTGAACCCCTCCAGGGGGGTGAATTATCATGATACTCGACACATATTGTT
GATCCTTAGAATTTCGAGCA

Downstream 100 bases:

>100_bases
TTCAAGAATAAAGAAAATCGCCCTGGTTGGTAACTATATTCCGCGCCAATGCGGTATAGCCACTTTCACGTCCGATCTTC
TTACGGCATTGGCCACGGAA

Product: GTP pyrophosphokinase / guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MGRDTQVLACGSLFIEKIENTYTAKDANLLKKAYAFSRQRESDCDSSSFKAAELLIEQGADGETVACALVAPHFWQGRVK
PEEIREHVSQDVADTLAYLKQPFSLRIDTEIHRRKDINALLVSMSETPRAAILLIVFRLIELETTLESQGKNPCHMAQET
LHFYVPIADRLSLGEMRRRLEDVCFRILHPFQYEKLKQDVTPIQSEDEKCLEILIEGVKRLLDKNRIHAEVHGRAKSLYS
IHLKMTLKGTALEDIMDRIGLRIIVSSVPECYAVLGLLHTHFKPIPGTFDDYIGLPKDNGYQSLHTCVYPMREVTHKPIE
FQVRTELMHMEAEHGSAAHWLYKSAVAMGKDSLKNQWLKGLVRRHDQAKSTDAFIELLRRQVCEDHMVVFGKGGRITRLP
DKATVLEYLDAANFFASRSSVVKVNGKLASLDQTLRDGDSIEIVDCEDSASPGTVVDDMGDIIGQHKASPMNTEGCKVWA
TAQGSILRHKEENSHASTEGP

Sequences:

>Translated_501_residues
MGRDTQVLACGSLFIEKIENTYTAKDANLLKKAYAFSRQRESDCDSSSFKAAELLIEQGADGETVACALVAPHFWQGRVK
PEEIREHVSQDVADTLAYLKQPFSLRIDTEIHRRKDINALLVSMSETPRAAILLIVFRLIELETTLESQGKNPCHMAQET
LHFYVPIADRLSLGEMRRRLEDVCFRILHPFQYEKLKQDVTPIQSEDEKCLEILIEGVKRLLDKNRIHAEVHGRAKSLYS
IHLKMTLKGTALEDIMDRIGLRIIVSSVPECYAVLGLLHTHFKPIPGTFDDYIGLPKDNGYQSLHTCVYPMREVTHKPIE
FQVRTELMHMEAEHGSAAHWLYKSAVAMGKDSLKNQWLKGLVRRHDQAKSTDAFIELLRRQVCEDHMVVFGKGGRITRLP
DKATVLEYLDAANFFASRSSVVKVNGKLASLDQTLRDGDSIEIVDCEDSASPGTVVDDMGDIIGQHKASPMNTEGCKVWA
TAQGSILRHKEENSHASTEGP
>Mature_500_residues
GRDTQVLACGSLFIEKIENTYTAKDANLLKKAYAFSRQRESDCDSSSFKAAELLIEQGADGETVACALVAPHFWQGRVKP
EEIREHVSQDVADTLAYLKQPFSLRIDTEIHRRKDINALLVSMSETPRAAILLIVFRLIELETTLESQGKNPCHMAQETL
HFYVPIADRLSLGEMRRRLEDVCFRILHPFQYEKLKQDVTPIQSEDEKCLEILIEGVKRLLDKNRIHAEVHGRAKSLYSI
HLKMTLKGTALEDIMDRIGLRIIVSSVPECYAVLGLLHTHFKPIPGTFDDYIGLPKDNGYQSLHTCVYPMREVTHKPIEF
QVRTELMHMEAEHGSAAHWLYKSAVAMGKDSLKNQWLKGLVRRHDQAKSTDAFIELLRRQVCEDHMVVFGKGGRITRLPD
KATVLEYLDAANFFASRSSVVKVNGKLASLDQTLRDGDSIEIVDCEDSASPGTVVDDMGDIIGQHKASPMNTEGCKVWAT
AQGSILRHKEENSHASTEGP

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=440, Percent_Identity=28.1818181818182, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1790082, Length=446, Percent_Identity=29.372197309417, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 56391; Mature: 56260

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRDTQVLACGSLFIEKIENTYTAKDANLLKKAYAFSRQRESDCDSSSFKAAELLIEQGA
CCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
DGETVACALVAPHFWQGRVKPEEIREHVSQDVADTLAYLKQPFSLRIDTEIHRRKDINAL
CCCEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCHHH
LVSMSETPRAAILLIVFRLIELETTLESQGKNPCHMAQETLHFYVPIADRLSLGEMRRRL
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECHHHHCCHHHHHHHH
EDVCFRILHPFQYEKLKQDVTPIQSEDEKCLEILIEGVKRLLDKNRIHAEVHGRAKSLYS
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEE
IHLKMTLKGTALEDIMDRIGLRIIVSSVPECYAVLGLLHTHFKPIPGTFDDYIGLPKDNG
EEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
YQSLHTCVYPMREVTHKPIEFQVRTELMHMEAEHGSAAHWLYKSAVAMGKDSLKNQWLKG
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
LVRRHDQAKSTDAFIELLRRQVCEDHMVVFGKGGRITRLPDKATVLEYLDAANFFASRSS
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCC
VVKVNGKLASLDQTLRDGDSIEIVDCEDSASPGTVVDDMGDIIGQHKASPMNTEGCKVWA
EEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEE
TAQGSILRHKEENSHASTEGP
ECCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GRDTQVLACGSLFIEKIENTYTAKDANLLKKAYAFSRQRESDCDSSSFKAAELLIEQGA
CCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
DGETVACALVAPHFWQGRVKPEEIREHVSQDVADTLAYLKQPFSLRIDTEIHRRKDINAL
CCCEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCHHH
LVSMSETPRAAILLIVFRLIELETTLESQGKNPCHMAQETLHFYVPIADRLSLGEMRRRL
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECHHHHCCHHHHHHHH
EDVCFRILHPFQYEKLKQDVTPIQSEDEKCLEILIEGVKRLLDKNRIHAEVHGRAKSLYS
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEE
IHLKMTLKGTALEDIMDRIGLRIIVSSVPECYAVLGLLHTHFKPIPGTFDDYIGLPKDNG
EEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
YQSLHTCVYPMREVTHKPIEFQVRTELMHMEAEHGSAAHWLYKSAVAMGKDSLKNQWLKG
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
LVRRHDQAKSTDAFIELLRRQVCEDHMVVFGKGGRITRLPDKATVLEYLDAANFFASRSS
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCC
VVKVNGKLASLDQTLRDGDSIEIVDCEDSASPGTVVDDMGDIIGQHKASPMNTEGCKVWA
EEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEE
TAQGSILRHKEENSHASTEGP
ECCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]