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The map label for this gene is rph

Identifier: 85859450

GI number: 85859450

Start: 1656484

End: 1657206

Strand: Direct

Name: rph

Synonym: SYN_00902

Alternate gene names: 85859450

Gene position: 1656484-1657206 (Clockwise)

Preceding gene: 85859449

Following gene: 85859451

Centisome position: 52.1

GC content: 50.9

Gene sequence:

>723_bases
TTGAGGAAAAATCGTCGTCATGAGGATGAACTTCGGGAAGTGAAAATCACCCGGAATTATTTGAAGCACCCCCAGGGGTC
AGTCCTCATTGAAGTCGGGGATACAAAGGTAATCTGTACAGCGGCATTGGAAGCAAAGGTCCCGCCCTTTCTGAGAAATT
CAGGAAAAGGTTGGTTGACAGCTGAATATGCGATGCTTCCCATGGCTACCCAGGAACGAAACCAGAGGGAATCTGTGCGG
GGCCATATCGGGGGAAGAACTCATGAAATCCAGAGGCTGATTGGCAGATCCCTTCGAGCAGTAACCGATCTGGACAGTTT
GGGCGAGAATACCGTCTACATTGACTGTGACGTCATTCAAGCTGATGGGGGAACAAGAACAGCGTCCATCACAGGAAGTT
TTGTGGCCTTGGTTGATCTTTTCCGAAAAATGAAGGAAAAGGGCATCTGCGAGACCATGCCGATACGGGATTACGTGTCC
GCAGTAAGTGTGGGCGTCGTTGACGGTCAACTCCTGCTTGATTTGGAATACGAAGAGGATTCGCGTGCGGACGTGGATAT
GAATGTGGTCATGACGGGCGCAGGCGAATTTATTGAAGTGCAGGGAACGGCGGAAAGGGTTCCTTTCGGGCGGGACAGAC
TGACCGCAATGGTCGAACTGGCTGGAAGAGGAATCAGAAAGTTGACGGAAGTTCAGAGAGAGATCCTGGGAGGACAGATC
TGA

Upstream 100 bases:

>100_bases
TATTGCCGACGCGATCAGCAGGAGCATAAAAGAACTTTACTGATCACGGGTCTTTTTTTCAGCGTCTCATTCGTAACTTT
TATACAGGGGCATCGGAATA

Downstream 100 bases:

>100_bases
GAAAAAAAGTCGTTTTCGCATCCCGCAATGAAGGAAAGATCGGGGAAGTACGTGCAATCCTTGAAGGTCTTGATATCGAT
CTGGTTTCATTGGCAGAGTA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRKNRRHEDELREVKITRNYLKHPQGSVLIEVGDTKVICTAALEAKVPPFLRNSGKGWLTAEYAMLPMATQERNQRESVR
GHIGGRTHEIQRLIGRSLRAVTDLDSLGENTVYIDCDVIQADGGTRTASITGSFVALVDLFRKMKEKGICETMPIRDYVS
AVSVGVVDGQLLLDLEYEEDSRADVDMNVVMTGAGEFIEVQGTAERVPFGRDRLTAMVELAGRGIRKLTEVQREILGGQI

Sequences:

>Translated_240_residues
MRKNRRHEDELREVKITRNYLKHPQGSVLIEVGDTKVICTAALEAKVPPFLRNSGKGWLTAEYAMLPMATQERNQRESVR
GHIGGRTHEIQRLIGRSLRAVTDLDSLGENTVYIDCDVIQADGGTRTASITGSFVALVDLFRKMKEKGICETMPIRDYVS
AVSVGVVDGQLLLDLEYEEDSRADVDMNVVMTGAGEFIEVQGTAERVPFGRDRLTAMVELAGRGIRKLTEVQREILGGQI
>Mature_240_residues
MRKNRRHEDELREVKITRNYLKHPQGSVLIEVGDTKVICTAALEAKVPPFLRNSGKGWLTAEYAMLPMATQERNQRESVR
GHIGGRTHEIQRLIGRSLRAVTDLDSLGENTVYIDCDVIQADGGTRTASITGSFVALVDLFRKMKEKGICETMPIRDYVS
AVSVGVVDGQLLLDLEYEEDSRADVDMNVVMTGAGEFIEVQGTAERVPFGRDRLTAMVELAGRGIRKLTEVQREILGGQI

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=60.2870813397129, Blast_Score=261, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_SYNAS (Q2LTS3)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461652.1
- ProteinModelPortal:   Q2LTS3
- SMR:   Q2LTS3
- STRING:   Q2LTS3
- GeneID:   3885010
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00902
- NMPDR:   fig|56780.10.peg.1595
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q2LTS3
- BioCyc:   SACI56780:SYN_00902-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26688; Mature: 26688

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNRRHEDELREVKITRNYLKHPQGSVLIEVGDTKVICTAALEAKVPPFLRNSGKGWLT
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEHHCCCCHHHHCCCCCEEE
AEYAMLPMATQERNQRESVRGHIGGRTHEIQRLIGRSLRAVTDLDSLGENTVYIDCDVIQ
EEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGSFVALVDLFRKMKEKGICETMPIRDYVSAVSVGVVDGQLLLDLEYEED
CCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCEEEEEEECCCC
SRADVDMNVVMTGAGEFIEVQGTAERVPFGRDRLTAMVELAGRGIRKLTEVQREILGGQI
CCCCCEEEEEEECCCCEEEECCCHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKNRRHEDELREVKITRNYLKHPQGSVLIEVGDTKVICTAALEAKVPPFLRNSGKGWLT
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEHHCCCCHHHHCCCCCEEE
AEYAMLPMATQERNQRESVRGHIGGRTHEIQRLIGRSLRAVTDLDSLGENTVYIDCDVIQ
EEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGSFVALVDLFRKMKEKGICETMPIRDYVSAVSVGVVDGQLLLDLEYEED
CCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCEEEEEEECCCC
SRADVDMNVVMTGAGEFIEVQGTAERVPFGRDRLTAMVELAGRGIRKLTEVQREILGGQI
CCCCCEEEEEEECCCCEEEECCCHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA