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The map label for this gene is purB [H]

Identifier: 85859074

GI number: 85859074

Start: 1249324

End: 1250622

Strand: Direct

Name: purB [H]

Synonym: SYN_00426

Alternate gene names: 85859074

Gene position: 1249324-1250622 (Clockwise)

Preceding gene: 85859062

Following gene: 85859075

Centisome position: 39.3

GC content: 53.35

Gene sequence:

>1299_bases
TTGATTCCAAGATATTCAAGAGATCGAATGACTGCCATCTGGACTCCGGAAAACAGGTACCGGACATGGCTGGATATTGA
AATCCTTGCCTGCGAGGCAATGTCTCAACTGGGCTTGATCCCTCCGGCTTCACTTGAACAAATCAAGAATAGAGCCGGGT
TTGATGTTGAAAGGATCGATGAGATTGAAAGGACGACCAAACATGATGTCATCGCCTTTTTAACGTCCGTTACTGAAAAG
GTCGGGGAGGACGGACGGTACATCCACATGGGAATGACCTCGTCCGATGTTCTGGATACTTCCTTTGCGGTATTGCTCAA
GCAGGCCGCGGACATCCTCATCGAAGACCTGGATCGGCTCCTGATTGTCCTGAAGGAGAAGGCTTTTCAGTTCAAGGACA
CCGTCATGATGGGCCGTTCCCACGGCATCCATGCGGAACCTGTCACCTTCGGCCTGAAAATGGCTCTCTGGTATCAGGAG
ATGATCCGCGGTCATGAGCGTCTCGTCAGAGCCAGAGACGCCGTCAGTGTCGGGAAGATATCCGGCGCAGTAGGAACGTT
CTCTTTTATTGATCCGGCGGTAGAGGCTTATGTCTGCCGCCATCTCGGATTGACGCCCGCCCCCATCTCATCTCAGATCG
TCCAGCGGGACCGCCATGCGGAATTCTTTTCCGTGCTGGCGATACTGGCTTCTTCGATCGACAAATTCTCCCAGGAGATC
CGCCTGCTCCAGAGAACCGAGGTGAGGGAGGCAGAAGAGTATTTTTCTCCGGGGCAGAAAGGATCGTCAGCGATGCCTCA
TAAACGGAACCCGGTTCTTTCCGAAAATCTTTCCGGGCTGGCCCGCCTGATGCGGTCCTACGCCCTGGCGGCTCTTGAGG
ATGTCCCGCTGTGGCATGAGCGGGATATCAGCCATTCCTCCGTGGAAAGAGTGATCGGACCCGATGCCACGATCCTTATG
GATTTCATGCTGGGCCGCTTTACCGGCCTGATGGAAAAACTTGTCGTCTACCCGGAACGGATGCTTTCCAACCTGAACAT
GACCCACGGCGTCATCTTCTCTCAAATGGTCCTGCTGCGTCTGATTGACAAGGGAATGACCCGCGAACAGGGTTATGCCG
TCGTCCAGAAGAATGCCATGACGGCCTGGCAGGAGGGGACTTCCTTTCAGGGATTGCTGATGCAGGACAACGAGGTGAGG
GGCTGCCTTACTGAAGAGGAACTGTGCGATGTCTTCCGGATTGAAAATTTCCTGAAAAATGTGGATGTCATCTTCAAGAG
GGTTTTTGGAGAACAATAG

Upstream 100 bases:

>100_bases
TTGAAGGATGGGTAGTTTTTAATTCCTTTTTACGGGTTTTTGGTTTATGAGGGAAATCCGGTTTTCATGGAAAAATTGAA
TGTAGAGAAGGAGAACGAGT

Downstream 100 bases:

>100_bases
GATGTCCCAGTGCCGGATTGTCTTTCTGGTCCTGCTGGTTGCCGCTGTTGTGGGTTGCGGTTCCATAAAAGGATACGTCG
ACATCGCCAGAGAACAGGGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIPRYSRDRMTAIWTPENRYRTWLDIEILACEAMSQLGLIPPASLEQIKNRAGFDVERIDEIERTTKHDVIAFLTSVTEK
VGEDGRYIHMGMTSSDVLDTSFAVLLKQAADILIEDLDRLLIVLKEKAFQFKDTVMMGRSHGIHAEPVTFGLKMALWYQE
MIRGHERLVRARDAVSVGKISGAVGTFSFIDPAVEAYVCRHLGLTPAPISSQIVQRDRHAEFFSVLAILASSIDKFSQEI
RLLQRTEVREAEEYFSPGQKGSSAMPHKRNPVLSENLSGLARLMRSYALAALEDVPLWHERDISHSSVERVIGPDATILM
DFMLGRFTGLMEKLVVYPERMLSNLNMTHGVIFSQMVLLRLIDKGMTREQGYAVVQKNAMTAWQEGTSFQGLLMQDNEVR
GCLTEEELCDVFRIENFLKNVDVIFKRVFGEQ

Sequences:

>Translated_432_residues
MIPRYSRDRMTAIWTPENRYRTWLDIEILACEAMSQLGLIPPASLEQIKNRAGFDVERIDEIERTTKHDVIAFLTSVTEK
VGEDGRYIHMGMTSSDVLDTSFAVLLKQAADILIEDLDRLLIVLKEKAFQFKDTVMMGRSHGIHAEPVTFGLKMALWYQE
MIRGHERLVRARDAVSVGKISGAVGTFSFIDPAVEAYVCRHLGLTPAPISSQIVQRDRHAEFFSVLAILASSIDKFSQEI
RLLQRTEVREAEEYFSPGQKGSSAMPHKRNPVLSENLSGLARLMRSYALAALEDVPLWHERDISHSSVERVIGPDATILM
DFMLGRFTGLMEKLVVYPERMLSNLNMTHGVIFSQMVLLRLIDKGMTREQGYAVVQKNAMTAWQEGTSFQGLLMQDNEVR
GCLTEEELCDVFRIENFLKNVDVIFKRVFGEQ
>Mature_432_residues
MIPRYSRDRMTAIWTPENRYRTWLDIEILACEAMSQLGLIPPASLEQIKNRAGFDVERIDEIERTTKHDVIAFLTSVTEK
VGEDGRYIHMGMTSSDVLDTSFAVLLKQAADILIEDLDRLLIVLKEKAFQFKDTVMMGRSHGIHAEPVTFGLKMALWYQE
MIRGHERLVRARDAVSVGKISGAVGTFSFIDPAVEAYVCRHLGLTPAPISSQIVQRDRHAEFFSVLAILASSIDKFSQEI
RLLQRTEVREAEEYFSPGQKGSSAMPHKRNPVLSENLSGLARLMRSYALAALEDVPLWHERDISHSSVERVIGPDATILM
DFMLGRFTGLMEKLVVYPERMLSNLNMTHGVIFSQMVLLRLIDKGMTREQGYAVVQKNAMTAWQEGTSFQGLLMQDNEVR
GCLTEEELCDVFRIENFLKNVDVIFKRVFGEQ

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=403, Percent_Identity=29.0322580645161, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI183227688, Length=382, Percent_Identity=29.3193717277487, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1787376, Length=277, Percent_Identity=33.5740072202166, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17508577, Length=399, Percent_Identity=27.8195488721804, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32564234, Length=339, Percent_Identity=28.9085545722714, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=384, Percent_Identity=26.8229166666667, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24647570, Length=380, Percent_Identity=27.1052631578947, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49067; Mature: 49067

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRDRMTAIWTPENRYRTWLDIEILACEAMSQLGLIPPASLEQIKNRAGFDVERID
CCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
EIERTTKHDVIAFLTSVTEKVGEDGRYIHMGMTSSDVLDTSFAVLLKQAADILIEDLDRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LIVLKEKAFQFKDTVMMGRSHGIHAEPVTFGLKMALWYQEMIRGHERLVRARDAVSVGKI
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFSFIDPAVEAYVCRHLGLTPAPISSQIVQRDRHAEFFSVLAILASSIDKFSQEI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLQRTEVREAEEYFSPGQKGSSAMPHKRNPVLSENLSGLARLMRSYALAALEDVPLWHE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSVERVIGPDATILMDFMLGRFTGLMEKLVVYPERMLSNLNMTHGVIFSQMVLLR
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LIDKGMTREQGYAVVQKNAMTAWQEGTSFQGLLMQDNEVRGCLTEEELCDVFRIENFLKN
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
VDVIFKRVFGEQ
HHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRDRMTAIWTPENRYRTWLDIEILACEAMSQLGLIPPASLEQIKNRAGFDVERID
CCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
EIERTTKHDVIAFLTSVTEKVGEDGRYIHMGMTSSDVLDTSFAVLLKQAADILIEDLDRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LIVLKEKAFQFKDTVMMGRSHGIHAEPVTFGLKMALWYQEMIRGHERLVRARDAVSVGKI
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFSFIDPAVEAYVCRHLGLTPAPISSQIVQRDRHAEFFSVLAILASSIDKFSQEI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLQRTEVREAEEYFSPGQKGSSAMPHKRNPVLSENLSGLARLMRSYALAALEDVPLWHE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSVERVIGPDATILMDFMLGRFTGLMEKLVVYPERMLSNLNMTHGVIFSQMVLLR
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LIDKGMTREQGYAVVQKNAMTAWQEGTSFQGLLMQDNEVRGCLTEEELCDVFRIENFLKN
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
VDVIFKRVFGEQ
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]