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The map label for this gene is ptsN [H]

Identifier: 85859060

GI number: 85859060

Start: 1232779

End: 1233240

Strand: Direct

Name: ptsN [H]

Synonym: SYN_00943

Alternate gene names: 85859060

Gene position: 1232779-1233240 (Clockwise)

Preceding gene: 85859059

Following gene: 85859061

Centisome position: 38.78

GC content: 42.42

Gene sequence:

>462_bases
ATGAAAATCACTGATTTGCTTAAAAAAGACTTTATCATCGAAGAGCTGGTCTCAACAGGCAAGCAGGAAGTGCTTGAAGA
GTTGTCGCGGAGTTTTCAGAAAGGAAATGTAAAGTTTAACCTGGATGCCATGATTAAAGTCCTTCTGGAACGTGAAAAAC
TTGGAAGCACCGGAATCGGTGATCATATTGCTATTCCTCACGGGAAATTGAATGGTCTTGACGACCTGATCGTCGCGTTC
GGACGAAGCAGCCGGGGGATCGATTTTGACGCGATGGATGGAAAACCGGTAAATTTGTTTTTTCTTCTGATGGCTCCGGA
ACACTCCACCGGTCAGCATCTTAAGGCGCTGGCCAAAATATCACGAATGTTGAAGGATCATCATTTTCGAAAAAGTCTTA
TGGAAACCAAATCCAAAGATGAATTATATCAGATTATTGCCGACAAGGATGATCAAACATAA

Upstream 100 bases:

>100_bases
ATGCGATATCCGAAAACGTCAATGTCATTTACAGAAGGGATGATGGCGGTTATCTCCTGATCGAAACCAACGGTTGATAT
CCTGTGCTTGAGGATCCGGC

Downstream 100 bases:

>100_bases
TTCCGGTTCCATATCAACGCGCATTTTCCGGCAGTTCCGTTATCGGTTTATCAGTGCACTACAGTATTGACTGAGCCTCC
CCTGCCTTGTCGCCTTGATA

Product: nitrogen regulatory IIA protein

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MKITDLLKKDFIIEELVSTGKQEVLEELSRSFQKGNVKFNLDAMIKVLLEREKLGSTGIGDHIAIPHGKLNGLDDLIVAF
GRSSRGIDFDAMDGKPVNLFFLLMAPEHSTGQHLKALAKISRMLKDHHFRKSLMETKSKDELYQIIADKDDQT

Sequences:

>Translated_153_residues
MKITDLLKKDFIIEELVSTGKQEVLEELSRSFQKGNVKFNLDAMIKVLLEREKLGSTGIGDHIAIPHGKLNGLDDLIVAF
GRSSRGIDFDAMDGKPVNLFFLLMAPEHSTGQHLKALAKISRMLKDHHFRKSLMETKSKDELYQIIADKDDQT
>Mature_153_residues
MKITDLLKKDFIIEELVSTGKQEVLEELSRSFQKGNVKFNLDAMIKVLLEREKLGSTGIGDHIAIPHGKLNGLDDLIVAF
GRSSRGIDFDAMDGKPVNLFFLLMAPEHSTGQHLKALAKISRMLKDHHFRKSLMETKSKDELYQIIADKDDQT

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=153, Percent_Identity=34.640522875817, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786951, Length=151, Percent_Identity=25.1655629139073, Blast_Score=63, Evalue=8e-12,
Organism=Escherichia coli, GI2367327, Length=117, Percent_Identity=30.7692307692308, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17265; Mature: 17265

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITDLLKKDFIIEELVSTGKQEVLEELSRSFQKGNVKFNLDAMIKVLLEREKLGSTGIG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCCCC
DHIAIPHGKLNGLDDLIVAFGRSSRGIDFDAMDGKPVNLFFLLMAPEHSTGQHLKALAKI
CCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
SRMLKDHHFRKSLMETKSKDELYQIIADKDDQT
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKITDLLKKDFIIEELVSTGKQEVLEELSRSFQKGNVKFNLDAMIKVLLEREKLGSTGIG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCCCC
DHIAIPHGKLNGLDDLIVAFGRSSRGIDFDAMDGKPVNLFFLLMAPEHSTGQHLKALAKI
CCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
SRMLKDHHFRKSLMETKSKDELYQIIADKDDQT
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]