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The map label for this gene is rpoN [H]

Identifier: 85859058

GI number: 85859058

Start: 1230643

End: 1232106

Strand: Direct

Name: rpoN [H]

Synonym: SYN_00945

Alternate gene names: 85859058

Gene position: 1230643-1232106 (Clockwise)

Preceding gene: 85859057

Following gene: 85859059

Centisome position: 38.71

GC content: 45.15

Gene sequence:

>1464_bases
ATGGCATTTGAACTCAAACAGAACCTCAAACTCAGTCAGCAACTGATCATGACTCCACAGCTGCAGCAGGCCATCAAGCT
GCTCCAGCTCTCAAGAATGGAAATGATCGATGTCATTAACCAGGAAATGGAAGAGAATCCTTTGCTGGAAGATGCACCTG
CGGATGAGTATGATGAAACGGAACGGAATCATGGCAGCGAGGAAGGCAAAACTCCTGAAAGGGAAGAGCTCAGGGGGATT
GAACGAACCGAAGAGATAACGGGAGAGGGAGACGGAAAAGAAGAATTCGACTGGAACAGTTACCTCGAAGATTATGGAAC
GTTCGGGACAACGTATGATCGAAGTGACGGGGAGGCTCCGAACTGGGATAATGTACTGACGGAAAAGTCGACATTGACCG
ATCATTTGATGTGGCAATTGAAGCTTTCCCGCTTGACGGAACAGGAAATGAGGGTCGGTGAACAGATAATCGGCAATCTG
GACGCGAATGGATATCTTGCCGCCTCTATTGAAGAAATTGCCGGGCTGGAAAACGTTTCGGAGAAAGACGTTGAAACGGT
TCTGAAAAAGATTCAGGAATTTGATCCCCCCGGGATTGCGGCAAGGAATCTTCAGGAATGCCTGCTTATCCAGGTCAAAA
TGTTAGGGATACAGAATCAACTGGTTGAGGCTATCATTCAGGATCACATGAAAGACCTGGAGATTAAGAATTACAATCAT
ATTTCCCGGAAAAACAAGGTCCCGGTCGATGATGTTTTTCAGGCGGTTATGATCATCAGCAACATGGATCCAAGACCCGG
AAGTGTTTATAATGAAGAGAAAATTCAGGCCATTATTCCTGACGTTTATGTCTTCAAGTCTGGTGATGATTATAAAATTG
TTCTGAATGATGACGGTTTGCCCCGCCTGAGGATCAGCAATTTCTATAAAGAAATCATGGGCAACGTCAAAGGCGATTCC
AATTCGGAAAGCGGCAGAAAATATATTCGAGAAAGAGTGCAGTCGGCAACCTGGCTGATTAAAAGCATTCAGCAGAGGCA
GAGAACCATATACAAAGTGGCGGAAAGCATCGTCAGGCACCAGAGGGAGTTTTTTGACAGAGGGATTAATTATCTGAAGC
CGCTGGTTTTACGAGATATCGCTTATGACGTGGAAATGCACGAGTCGACGATCAGCAGAGTGGTAACAAATAAATACATG
TACACCCCTCGCGGGATCTTCGAATTGAAATATTTTTTCAGCAGCAGTATTCAACGGAAAAGTGGCGAAGCTATCGCGTC
AAAAAGTGTCAAAGAGGAAATCCGCAGGGTCATCGGCGCGGAAGATCCTCTACATCCCTACAGCGATCAGGAAATTGTCA
GTATTCTGGAGAGTAAGGGTATTAAAATTGCCCGGAGAACCGTTGCCAAATATCGAGAAATGATGAATATTCTGCCTTCA
TCCAGAAGGAGAAGGTATTTTTAA

Upstream 100 bases:

>100_bases
TCCAGTGAAATGGCAAGGAAAATTTATTTCGGCGAGGATTTTACTCTGTAATTGATCTTCGGCGACAGCGTGCATTTCAT
ATTGGCAGAAGTTAAAAGAT

Downstream 100 bases:

>100_bases
CCTGGAAACATAACGTTCGTTGATGTTCCCGTTTCGGGTTAAGCCGTTCGGCCGTATCATCATGTTACGGCCGTATCACG
TTAAATGGATTCAAGGAGGA

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MAFELKQNLKLSQQLIMTPQLQQAIKLLQLSRMEMIDVINQEMEENPLLEDAPADEYDETERNHGSEEGKTPEREELRGI
ERTEEITGEGDGKEEFDWNSYLEDYGTFGTTYDRSDGEAPNWDNVLTEKSTLTDHLMWQLKLSRLTEQEMRVGEQIIGNL
DANGYLAASIEEIAGLENVSEKDVETVLKKIQEFDPPGIAARNLQECLLIQVKMLGIQNQLVEAIIQDHMKDLEIKNYNH
ISRKNKVPVDDVFQAVMIISNMDPRPGSVYNEEKIQAIIPDVYVFKSGDDYKIVLNDDGLPRLRISNFYKEIMGNVKGDS
NSESGRKYIRERVQSATWLIKSIQQRQRTIYKVAESIVRHQREFFDRGINYLKPLVLRDIAYDVEMHESTISRVVTNKYM
YTPRGIFELKYFFSSSIQRKSGEAIASKSVKEEIRRVIGAEDPLHPYSDQEIVSILESKGIKIARRTVAKYREMMNILPS
SRRRRYF

Sequences:

>Translated_487_residues
MAFELKQNLKLSQQLIMTPQLQQAIKLLQLSRMEMIDVINQEMEENPLLEDAPADEYDETERNHGSEEGKTPEREELRGI
ERTEEITGEGDGKEEFDWNSYLEDYGTFGTTYDRSDGEAPNWDNVLTEKSTLTDHLMWQLKLSRLTEQEMRVGEQIIGNL
DANGYLAASIEEIAGLENVSEKDVETVLKKIQEFDPPGIAARNLQECLLIQVKMLGIQNQLVEAIIQDHMKDLEIKNYNH
ISRKNKVPVDDVFQAVMIISNMDPRPGSVYNEEKIQAIIPDVYVFKSGDDYKIVLNDDGLPRLRISNFYKEIMGNVKGDS
NSESGRKYIRERVQSATWLIKSIQQRQRTIYKVAESIVRHQREFFDRGINYLKPLVLRDIAYDVEMHESTISRVVTNKYM
YTPRGIFELKYFFSSSIQRKSGEAIASKSVKEEIRRVIGAEDPLHPYSDQEIVSILESKGIKIARRTVAKYREMMNILPS
SRRRRYF
>Mature_486_residues
AFELKQNLKLSQQLIMTPQLQQAIKLLQLSRMEMIDVINQEMEENPLLEDAPADEYDETERNHGSEEGKTPEREELRGIE
RTEEITGEGDGKEEFDWNSYLEDYGTFGTTYDRSDGEAPNWDNVLTEKSTLTDHLMWQLKLSRLTEQEMRVGEQIIGNLD
ANGYLAASIEEIAGLENVSEKDVETVLKKIQEFDPPGIAARNLQECLLIQVKMLGIQNQLVEAIIQDHMKDLEIKNYNHI
SRKNKVPVDDVFQAVMIISNMDPRPGSVYNEEKIQAIIPDVYVFKSGDDYKIVLNDDGLPRLRISNFYKEIMGNVKGDSN
SESGRKYIRERVQSATWLIKSIQQRQRTIYKVAESIVRHQREFFDRGINYLKPLVLRDIAYDVEMHESTISRVVTNKYMY
TPRGIFELKYFFSSSIQRKSGEAIASKSVKEEIRRVIGAEDPLHPYSDQEIVSILESKGIKIARRTVAKYREMMNILPSS
RRRRYF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=483, Percent_Identity=40.1656314699793, Blast_Score=355, Evalue=5e-99,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56412; Mature: 56281

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFELKQNLKLSQQLIMTPQLQQAIKLLQLSRMEMIDVINQEMEENPLLEDAPADEYDET
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
ERNHGSEEGKTPEREELRGIERTEEITGEGDGKEEFDWNSYLEDYGTFGTTYDRSDGEAP
HHHCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
NWDNVLTEKSTLTDHLMWQLKLSRLTEQEMRVGEQIIGNLDANGYLAASIEEIAGLENVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHCCCCCC
EKDVETVLKKIQEFDPPGIAARNLQECLLIQVKMLGIQNQLVEAIIQDHMKDLEIKNYNH
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ISRKNKVPVDDVFQAVMIISNMDPRPGSVYNEEKIQAIIPDVYVFKSGDDYKIVLNDDGL
HHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEECCCCC
PRLRISNFYKEIMGNVKGDSNSESGRKYIRERVQSATWLIKSIQQRQRTIYKVAESIVRH
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QREFFDRGINYLKPLVLRDIAYDVEMHESTISRVVTNKYMYTPRGIFELKYFFSSSIQRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHH
SGEAIASKSVKEEIRRVIGAEDPLHPYSDQEIVSILESKGIKIARRTVAKYREMMNILPS
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
SRRRRYF
CCCCCCC
>Mature Secondary Structure 
AFELKQNLKLSQQLIMTPQLQQAIKLLQLSRMEMIDVINQEMEENPLLEDAPADEYDET
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
ERNHGSEEGKTPEREELRGIERTEEITGEGDGKEEFDWNSYLEDYGTFGTTYDRSDGEAP
HHHCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
NWDNVLTEKSTLTDHLMWQLKLSRLTEQEMRVGEQIIGNLDANGYLAASIEEIAGLENVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHCCCCCC
EKDVETVLKKIQEFDPPGIAARNLQECLLIQVKMLGIQNQLVEAIIQDHMKDLEIKNYNH
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ISRKNKVPVDDVFQAVMIISNMDPRPGSVYNEEKIQAIIPDVYVFKSGDDYKIVLNDDGL
HHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEECCCCC
PRLRISNFYKEIMGNVKGDSNSESGRKYIRERVQSATWLIKSIQQRQRTIYKVAESIVRH
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QREFFDRGINYLKPLVLRDIAYDVEMHESTISRVVTNKYMYTPRGIFELKYFFSSSIQRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHH
SGEAIASKSVKEEIRRVIGAEDPLHPYSDQEIVSILESKGIKIARRTVAKYREMMNILPS
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
SRRRRYF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203540; 8444818; 8025669; 7876255; 9278503 [H]