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The map label for this gene is nuoF2 [H]

Identifier: 85858925

GI number: 85858925

Start: 1105371

End: 1107287

Strand: Direct

Name: nuoF2 [H]

Synonym: SYN_02139

Alternate gene names: 85858925

Gene position: 1105371-1107287 (Clockwise)

Preceding gene: 85858924

Following gene: 85858926

Centisome position: 34.77

GC content: 57.96

Gene sequence:

>1917_bases
TTGACGCGCTGGATCCGGTCTGCAAGATCCCCGAATATAAGGTCTGTTCTTGCAAAATCGAAAAAATTTACCTGTAAGGA
GGGGATAGCGATGATTACGACGGAAAATATCCAAGAGGTGATTAATAACCGGGGAAAGGCCAGGGAACACCTGATGGCCA
TTCTGCGGGATCTGGAAAATCTGTCCGGCAACCAGCAGTTGAGCCCGGAAACCCTGAACGCCGTCGCGGAGGCCATGAAC
CTCCCGCAGAGTACGGTGGCGGGCTTTGTCGGCTTTTATACGATGTTCAGCACCAGGCCCCGGGCGAAATTCCTGATCCG
GGTCTGCAAGTCCGGCCCCTGCCATGTCATGGGGGCGAGGACGATCTTCGATTATGTAGAAAAGCACCTGGGGATCTCGC
CCGGCCAGACAACGGCGGACGGACTGTTCCACCTGGAGGCTTGTGAATGCCTGGGCATCTGTTCCGTGGCTCCGGCGATG
ATGATCAACTACGATCTCCACGGCAACCTGACGGAAGAGCGGATTGCGACGATTCTGGACGGGTACCGGAGCAGGGAGCC
CTTCTTCGGCGAGGCCTGTGGCCCCGAGGTGGAAGGAAGAGTCTGCATGCTGGATGAACCCGGACAGACCCAGAGACTGA
CGGAGAAAATCGGCTCGATCGATCCCCTGAGCGTGGACAGCTATCTGGAAAAGGGTGGTTATGCAGCAATAAAGAAAGCC
CTGGCGGAATATCAGCCCGATGACGTGATCGCCATCGTCAAGGATTCCGGATTGCGTGGCCGCGGCGGTGCAGGATTCCC
CGCAGGCGTGAAATGGTCCTTCCTGCCCAAGGGGGACATGCAGAAGTACGTCATCTGCAATGCCGACGAAGGGGAGCCGG
GCACTTACAAGGACCGGATTCTCATGGAGGAAAATCCCCATGGACTCCTCGAAGGCATGATGCTCTGCGGCTATGCCACG
GGCGCCACGGTCGGGTATATCTACATCCGGGGTGAATACCGCCGCTCCATCGAGCGGCTCCAGCGGGCCATCGATCAGGC
CCGGGAAAAGGGCATCCTGGGGGATAACATTTTCGGGTCTTCCTTCCGCTTCGACATCTTCATCAAAGAAGGGGGCGGGG
CTTATGTCTGCGGCGAGGAATCATCGCTCATGAATTCCATGGAAGGAAAACGGGGCTACCCTCGCTTCCGGCCGCCTTTC
CCGGCAGGGGCCGGCTTCCTGGCGAAGCCCTCGAACGTCAACAACGTGGAAACCTATGCCTCCGTGCCGATGATTATCGA
GAAGGGTGCGGCCTGGTACAAATCCGTCGGCCTGGAAACATGTACGGGAACCAAACTGTACTGTCTCTCCGGCAAGCTGA
ACCGGACGGGGCTGGTGGAAATGCCCATGGGAACAACCCTGCGGGAAATCATCGAAACGTTCGGGAAGGGCATCAAGAAC
GGAAAGACCTTCAAGTTCGCCCAGGTGGGCGGTTCCGCCGGCGGTATCCTGGGGCCGGAACTGATGGATCTGCCGCTGGA
CATCGATTCAACGATCAAGGCGGGGGTGACGCTGGGTTCCGGCGTGGTACTGGTCTGTGACGAAGACACCTGTCCGGTGG
ATTTTCTCCTCGATGTCCTCAACTTCTTCGAACATGAATCCTGCGGTCAGTGCGTCCCCTGCCGCGTGGGAACCCGCCAG
CTTCATCATCTGGCCCGGAAGTTCGCCACTGGAACCGCGGTTCCGGGCGATCTGGACCTCATGGTGGAGAAGGCGAAACT
GATGAAAAACTCCCTTTGTGCACTCGGGCAGTCACCCATTCTTCCCATCACGACGATGCTGAAATATTTCCGGGAAGATT
TCCTCCGACACTGCGATCCCTCCTTATGCTGTCCGGAATGTGCCAACTCGCTGGCCCACTACTATCGGGGTCACTGA

Upstream 100 bases:

>100_bases
GGCAGCATCCGCATCAAGGCGAAAATATCGGAAAAGCCGAAAGCCGGCGTGGTCTTTATGCCCTTCCATTTCTTTGAGAC
CTGCGCGAATGTCCTGACGA

Downstream 100 bases:

>100_bases
GATCAGGTCATAGGCAAAGGGAAAGAATTTTTTTCTTGAATTAATTTCCCTTTGCCTATAAATGGGGAAAACCTATTTTA
CGATGGGAATTTTATATTTT

Product: NADH-quinone oxidoreductase chain F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MTRWIRSARSPNIRSVLAKSKKFTCKEGIAMITTENIQEVINNRGKAREHLMAILRDLENLSGNQQLSPETLNAVAEAMN
LPQSTVAGFVGFYTMFSTRPRAKFLIRVCKSGPCHVMGARTIFDYVEKHLGISPGQTTADGLFHLEACECLGICSVAPAM
MINYDLHGNLTEERIATILDGYRSREPFFGEACGPEVEGRVCMLDEPGQTQRLTEKIGSIDPLSVDSYLEKGGYAAIKKA
LAEYQPDDVIAIVKDSGLRGRGGAGFPAGVKWSFLPKGDMQKYVICNADEGEPGTYKDRILMEENPHGLLEGMMLCGYAT
GATVGYIYIRGEYRRSIERLQRAIDQAREKGILGDNIFGSSFRFDIFIKEGGGAYVCGEESSLMNSMEGKRGYPRFRPPF
PAGAGFLAKPSNVNNVETYASVPMIIEKGAAWYKSVGLETCTGTKLYCLSGKLNRTGLVEMPMGTTLREIIETFGKGIKN
GKTFKFAQVGGSAGGILGPELMDLPLDIDSTIKAGVTLGSGVVLVCDEDTCPVDFLLDVLNFFEHESCGQCVPCRVGTRQ
LHHLARKFATGTAVPGDLDLMVEKAKLMKNSLCALGQSPILPITTMLKYFREDFLRHCDPSLCCPECANSLAHYYRGH

Sequences:

>Translated_638_residues
MTRWIRSARSPNIRSVLAKSKKFTCKEGIAMITTENIQEVINNRGKAREHLMAILRDLENLSGNQQLSPETLNAVAEAMN
LPQSTVAGFVGFYTMFSTRPRAKFLIRVCKSGPCHVMGARTIFDYVEKHLGISPGQTTADGLFHLEACECLGICSVAPAM
MINYDLHGNLTEERIATILDGYRSREPFFGEACGPEVEGRVCMLDEPGQTQRLTEKIGSIDPLSVDSYLEKGGYAAIKKA
LAEYQPDDVIAIVKDSGLRGRGGAGFPAGVKWSFLPKGDMQKYVICNADEGEPGTYKDRILMEENPHGLLEGMMLCGYAT
GATVGYIYIRGEYRRSIERLQRAIDQAREKGILGDNIFGSSFRFDIFIKEGGGAYVCGEESSLMNSMEGKRGYPRFRPPF
PAGAGFLAKPSNVNNVETYASVPMIIEKGAAWYKSVGLETCTGTKLYCLSGKLNRTGLVEMPMGTTLREIIETFGKGIKN
GKTFKFAQVGGSAGGILGPELMDLPLDIDSTIKAGVTLGSGVVLVCDEDTCPVDFLLDVLNFFEHESCGQCVPCRVGTRQ
LHHLARKFATGTAVPGDLDLMVEKAKLMKNSLCALGQSPILPITTMLKYFREDFLRHCDPSLCCPECANSLAHYYRGH
>Mature_637_residues
TRWIRSARSPNIRSVLAKSKKFTCKEGIAMITTENIQEVINNRGKAREHLMAILRDLENLSGNQQLSPETLNAVAEAMNL
PQSTVAGFVGFYTMFSTRPRAKFLIRVCKSGPCHVMGARTIFDYVEKHLGISPGQTTADGLFHLEACECLGICSVAPAMM
INYDLHGNLTEERIATILDGYRSREPFFGEACGPEVEGRVCMLDEPGQTQRLTEKIGSIDPLSVDSYLEKGGYAAIKKAL
AEYQPDDVIAIVKDSGLRGRGGAGFPAGVKWSFLPKGDMQKYVICNADEGEPGTYKDRILMEENPHGLLEGMMLCGYATG
ATVGYIYIRGEYRRSIERLQRAIDQAREKGILGDNIFGSSFRFDIFIKEGGGAYVCGEESSLMNSMEGKRGYPRFRPPFP
AGAGFLAKPSNVNNVETYASVPMIIEKGAAWYKSVGLETCTGTKLYCLSGKLNRTGLVEMPMGTTLREIIETFGKGIKNG
KTFKFAQVGGSAGGILGPELMDLPLDIDSTIKAGVTLGSGVVLVCDEDTCPVDFLLDVLNFFEHESCGQCVPCRVGTRQL
HHLARKFATGTAVPGDLDLMVEKAKLMKNSLCALGQSPILPITTMLKYFREDFLRHCDPSLCCPECANSLAHYYRGH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=391, Percent_Identity=41.1764705882353, Blast_Score=316, Evalue=4e-86,
Organism=Homo sapiens, GI260656005, Length=391, Percent_Identity=41.1764705882353, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI222080062, Length=158, Percent_Identity=33.5443037974684, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1788620, Length=411, Percent_Identity=42.5790754257908, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788621, Length=112, Percent_Identity=41.0714285714286, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531727, Length=375, Percent_Identity=42.1333333333333, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17561328, Length=148, Percent_Identity=37.8378378378378, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19920794, Length=391, Percent_Identity=40.6649616368286, Blast_Score=314, Evalue=9e-86,
Organism=Drosophila melanogaster, GI45550452, Length=392, Percent_Identity=39.5408163265306, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24653819, Length=378, Percent_Identity=38.0952380952381, Blast_Score=254, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24653817, Length=348, Percent_Identity=36.2068965517241, Blast_Score=223, Evalue=4e-58,
Organism=Drosophila melanogaster, GI21355353, Length=132, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859877, Length=113, Percent_Identity=38.0530973451327, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 69859; Mature: 69728

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRWIRSARSPNIRSVLAKSKKFTCKEGIAMITTENIQEVINNRGKAREHLMAILRDLEN
CCHHHHHCCCCCHHHHHHHHCCCHHHCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHH
LSGNQQLSPETLNAVAEAMNLPQSTVAGFVGFYTMFSTRPRAKFLIRVCKSGPCHVMGAR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEHH
TIFDYVEKHLGISPGQTTADGLFHLEACECLGICSVAPAMMINYDLHGNLTEERIATILD
HHHHHHHHHCCCCCCCCCHHHEEEEHHHHHHHHHHHCHHHEEEECCCCCCHHHHHHHHHH
GYRSREPFFGEACGPEVEGRVCMLDEPGQTQRLTEKIGSIDPLSVDSYLEKGGYAAIKKA
HHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHH
LAEYQPDDVIAIVKDSGLRGRGGAGFPAGVKWSFLPKGDMQKYVICNADEGEPGTYKDRI
HHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCEE
LMEENPHGLLEGMMLCGYATGATVGYIYIRGEYRRSIERLQRAIDQAREKGILGDNIFGS
EEECCCCHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SFRFDIFIKEGGGAYVCGEESSLMNSMEGKRGYPRFRPPFPAGAGFLAKPSNVNNVETYA
CEEEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
SVPMIIEKGAAWYKSVGLETCTGTKLYCLSGKLNRTGLVEMPMGTTLREIIETFGKGIKN
HCCHHHHCCCHHHHHCCCCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
GKTFKFAQVGGSAGGILGPELMDLPLDIDSTIKAGVTLGSGVVLVCDEDTCPVDFLLDVL
CCEEEEEECCCCCCCCCCCHHHCCCCCCCCHHHHCEEECCCEEEEECCCCCCHHHHHHHH
NFFEHESCGQCVPCRVGTRQLHHLARKFATGTAVPGDLDLMVEKAKLMKNSLCALGQSPI
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LPITTMLKYFREDFLRHCDPSLCCPECANSLAHYYRGH
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRWIRSARSPNIRSVLAKSKKFTCKEGIAMITTENIQEVINNRGKAREHLMAILRDLEN
CHHHHHCCCCCHHHHHHHHCCCHHHCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHH
LSGNQQLSPETLNAVAEAMNLPQSTVAGFVGFYTMFSTRPRAKFLIRVCKSGPCHVMGAR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEHH
TIFDYVEKHLGISPGQTTADGLFHLEACECLGICSVAPAMMINYDLHGNLTEERIATILD
HHHHHHHHHCCCCCCCCCHHHEEEEHHHHHHHHHHHCHHHEEEECCCCCCHHHHHHHHHH
GYRSREPFFGEACGPEVEGRVCMLDEPGQTQRLTEKIGSIDPLSVDSYLEKGGYAAIKKA
HHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHH
LAEYQPDDVIAIVKDSGLRGRGGAGFPAGVKWSFLPKGDMQKYVICNADEGEPGTYKDRI
HHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCEE
LMEENPHGLLEGMMLCGYATGATVGYIYIRGEYRRSIERLQRAIDQAREKGILGDNIFGS
EEECCCCHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SFRFDIFIKEGGGAYVCGEESSLMNSMEGKRGYPRFRPPFPAGAGFLAKPSNVNNVETYA
CEEEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
SVPMIIEKGAAWYKSVGLETCTGTKLYCLSGKLNRTGLVEMPMGTTLREIIETFGKGIKN
HCCHHHHCCCHHHHHCCCCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
GKTFKFAQVGGSAGGILGPELMDLPLDIDSTIKAGVTLGSGVVLVCDEDTCPVDFLLDVL
CCEEEEEECCCCCCCCCCCHHHCCCCCCCCHHHHCEEECCCEEEEECCCCCCHHHHHHHH
NFFEHESCGQCVPCRVGTRQLHHLARKFATGTAVPGDLDLMVEKAKLMKNSLCALGQSPI
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LPITTMLKYFREDFLRHCDPSLCCPECANSLAHYYRGH
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]