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The map label for this gene is nifS1 [H]

Identifier: 85858913

GI number: 85858913

Start: 1092286

End: 1093470

Strand: Direct

Name: nifS1 [H]

Synonym: SYN_02126

Alternate gene names: 85858913

Gene position: 1092286-1093470 (Clockwise)

Preceding gene: 85858912

Following gene: 85858914

Centisome position: 34.36

GC content: 57.3

Gene sequence:

>1185_bases
ATGACGACGGTTTATCTGGATAACAATGCAACAACCCGTGTTGTGCCGGAGGTGCTGGAGGTGATGCTCCCCTATTTCAG
CGAGCTCTACGGCAATCCGTCAAGCATGCATACCTTTGGCGGGCAGGTGGGCATCAAAATCCGGGAGGCCCGGGAACAGG
TTGCCGCCTTGCTTGGCGCGTCTCCCGACGAGATCATTTTTACCAGCTGTGGTACGGAAAGCGACAATACGGCCATTCGA
TCGGCACTGGCCGCCCATCCGGATCGCAAGCATATCGTGACCAGTCGGGTGGAGCATCCAGCCATCAAAGCGCTCTGCGC
CCATCTGGCCGAATCGGGCTATCGTGTGACGGAACTGCCGGTCGACAGTGAGGGCCGTCTCAATATGGAAGCCTTTGAAC
AATGCCTGACGCCGGACACGGCCATCGTAAGCCTCATGTGGGCCAACAATGAAACAGGCGTTGTCTTTCCTGTGGAGAGG
GTCGCGGAGATCGCTCACAGCCACGGTATCCTGTTTCACACCGATGCCGTCCAGGCCGTCGGGAAAATTCCGATCGACAT
GCGGCAAAATACGATAGACATGCTGTCCCTTTCCGGTCACAAGCTCCATGCCCCCAAGGGGATCGGCGTCTTCTATCTCC
GGCGGGGAACGCGTTTTTCTCCCTTCATGATCGGCGGTCACCAGGAAAGGGAACGCCGAGGAGGGACGGAGAACACACCA
AGCATCATCGGTCTTGGAAAGGCCTGTGAACTGGCAGCCCGGCATATGGAAGAGGAGGACAGCAGGGTAAGGCAATTGCG
GGACAAACTGGAAAACGAACTTCTGGCGAGAATTCCCCGGGCCCGTGTTAACGGCGACCGGGTCAATCGTCTTCCCAACA
CCACCAACATCAGTTTTGAATTTGTGGAAGGGGAGGCCATCCTGCTTATGCTGGATCGATATGGCATTTGTGCCTCATCC
GGCTCGGCATGCACATCGGGATCTCTGCAACCGTCACACGTGCTGCGGGCCATGGGCGTTCCCTTCACTATGGCCCACGG
ATCGATTCGATTCAGTCTCAGCATCGAAAACACGGAAGCGGACGTCGATCTGGTCATTGAGACCCTGCCGGGAATCATCG
CGCAGCTGAGGGAAATGTCGCCGTTCTGGCAGACGGAAGGCCAATCGTGTGCCGCCAGTTCATGA

Upstream 100 bases:

>100_bases
GTGCCGTTTGCCGGGCCTCCCAGCAGACCCTGAAGGGTTTTGTGGAGTTCAAACTCCGTGAACTGGTAACACCAGAGCTC
ATCGTTGAGGAGGTGAAGGC

Downstream 100 bases:

>100_bases
CCATCTCGAAGTTCGCGGAGTAAAAATTATGGATAAGGATAAACGCCGTACAGCCCTGGTCACGGGCGCGAGCCGGGGGA
TCGGGCGCGCTGTGGCCCTT

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTTVYLDNNATTRVVPEVLEVMLPYFSELYGNPSSMHTFGGQVGIKIREAREQVAALLGASPDEIIFTSCGTESDNTAIR
SALAAHPDRKHIVTSRVEHPAIKALCAHLAESGYRVTELPVDSEGRLNMEAFEQCLTPDTAIVSLMWANNETGVVFPVER
VAEIAHSHGILFHTDAVQAVGKIPIDMRQNTIDMLSLSGHKLHAPKGIGVFYLRRGTRFSPFMIGGHQERERRGGTENTP
SIIGLGKACELAARHMEEEDSRVRQLRDKLENELLARIPRARVNGDRVNRLPNTTNISFEFVEGEAILLMLDRYGICASS
GSACTSGSLQPSHVLRAMGVPFTMAHGSIRFSLSIENTEADVDLVIETLPGIIAQLREMSPFWQTEGQSCAASS

Sequences:

>Translated_394_residues
MTTVYLDNNATTRVVPEVLEVMLPYFSELYGNPSSMHTFGGQVGIKIREAREQVAALLGASPDEIIFTSCGTESDNTAIR
SALAAHPDRKHIVTSRVEHPAIKALCAHLAESGYRVTELPVDSEGRLNMEAFEQCLTPDTAIVSLMWANNETGVVFPVER
VAEIAHSHGILFHTDAVQAVGKIPIDMRQNTIDMLSLSGHKLHAPKGIGVFYLRRGTRFSPFMIGGHQERERRGGTENTP
SIIGLGKACELAARHMEEEDSRVRQLRDKLENELLARIPRARVNGDRVNRLPNTTNISFEFVEGEAILLMLDRYGICASS
GSACTSGSLQPSHVLRAMGVPFTMAHGSIRFSLSIENTEADVDLVIETLPGIIAQLREMSPFWQTEGQSCAASS
>Mature_393_residues
TTVYLDNNATTRVVPEVLEVMLPYFSELYGNPSSMHTFGGQVGIKIREAREQVAALLGASPDEIIFTSCGTESDNTAIRS
ALAAHPDRKHIVTSRVEHPAIKALCAHLAESGYRVTELPVDSEGRLNMEAFEQCLTPDTAIVSLMWANNETGVVFPVERV
AEIAHSHGILFHTDAVQAVGKIPIDMRQNTIDMLSLSGHKLHAPKGIGVFYLRRGTRFSPFMIGGHQERERRGGTENTPS
IIGLGKACELAARHMEEEDSRVRQLRDKLENELLARIPRARVNGDRVNRLPNTTNISFEFVEGEAILLMLDRYGICASSG
SACTSGSLQPSHVLRAMGVPFTMAHGSIRFSLSIENTEADVDLVIETLPGIIAQLREMSPFWQTEGQSCAASS

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=43.8961038961039, Blast_Score=334, Evalue=9e-92,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=37.8896882494005, Blast_Score=237, Evalue=1e-62,
Organism=Escherichia coli, GI48994898, Length=397, Percent_Identity=42.5692695214106, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1789175, Length=391, Percent_Identity=29.6675191815857, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787970, Length=396, Percent_Identity=25.5050505050505, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=386, Percent_Identity=41.9689119170984, Blast_Score=307, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI17533177, Length=315, Percent_Identity=32.6984126984127, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=385, Percent_Identity=41.2987012987013, Blast_Score=308, Evalue=1e-84,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=42.3376623376623, Blast_Score=318, Evalue=5e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43179; Mature: 43048

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVYLDNNATTRVVPEVLEVMLPYFSELYGNPSSMHTFGGQVGIKIREAREQVAALLGA
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEEHHHHHHHHHHHCC
SPDEIIFTSCGTESDNTAIRSALAAHPDRKHIVTSRVEHPAIKALCAHLAESGYRVTELP
CCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECC
VDSEGRLNMEAFEQCLTPDTAIVSLMWANNETGVVFPVERVAEIAHSHGILFHTDAVQAV
CCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCEEEECHHHHHH
GKIPIDMRQNTIDMLSLSGHKLHAPKGIGVFYLRRGTRFSPFMIGGHQERERRGGTENTP
HCCCCCCCCCCEEEEECCCCEEECCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCCCC
SIIGLGKACELAARHMEEEDSRVRQLRDKLENELLARIPRARVNGDRVNRLPNTTNISFE
CEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEE
FVEGEAILLMLDRYGICASSGSACTSGSLQPSHVLRAMGVPFTMAHGSIRFSLSIENTEA
EECCCEEEEEEECCCEECCCCCCCCCCCCCHHHHHHHHCCCEEEECCEEEEEEEECCCCC
DVDLVIETLPGIIAQLREMSPFWQTEGQSCAASS
CHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCC
>Mature Secondary Structure 
TTVYLDNNATTRVVPEVLEVMLPYFSELYGNPSSMHTFGGQVGIKIREAREQVAALLGA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEEHHHHHHHHHHHCC
SPDEIIFTSCGTESDNTAIRSALAAHPDRKHIVTSRVEHPAIKALCAHLAESGYRVTELP
CCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECC
VDSEGRLNMEAFEQCLTPDTAIVSLMWANNETGVVFPVERVAEIAHSHGILFHTDAVQAV
CCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCEEEECHHHHHH
GKIPIDMRQNTIDMLSLSGHKLHAPKGIGVFYLRRGTRFSPFMIGGHQERERRGGTENTP
HCCCCCCCCCCEEEEECCCCEEECCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCCCC
SIIGLGKACELAARHMEEEDSRVRQLRDKLENELLARIPRARVNGDRVNRLPNTTNISFE
CEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEE
FVEGEAILLMLDRYGICASSGSACTSGSLQPSHVLRAMGVPFTMAHGSIRFSLSIENTEA
EECCCEEEEEEECCCEECCCCCCCCCCCCCHHHHHHHHCCCEEEECCEEEEEEEECCCCC
DVDLVIETLPGIIAQLREMSPFWQTEGQSCAASS
CHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]