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The map label for this gene is nifU [H]

Identifier: 85858912

GI number: 85858912

Start: 1091465

End: 1092289

Strand: Direct

Name: nifU [H]

Synonym: SYN_02125

Alternate gene names: 85858912

Gene position: 1091465-1092289 (Clockwise)

Preceding gene: 85858910

Following gene: 85858913

Centisome position: 34.33

GC content: 55.39

Gene sequence:

>825_bases
ATGTGGGAATATACGGATAAGGTCAAGGAACATTTTTTGAAGCCCCGGAATGTTGGGGAAGTGGAGAATCCGGACGGGGT
GGCGGAGGTCGGCTCAATGGCCTGTGGCGATGCGCTCCGGCTGACTTTCAAACTGGATGAAAACAAGCGGATCAAGGAGG
CGAAATTCAAGACCTTCGGCTGTGCCAGTGCCATTGCCGCGGCCTCGGCGATGACGGAGATGATCAAGGGCCTCACCGTG
GAAGAGGCTGAAAAGATAACCAATCAGGACATTGCCGCTTTCCTTGGAGGGCTTCCTCAGGAAAAGATGCACTGTTCGGT
TCTCGGGCAGCAGGCTCTGGAAAAGGCGATTGCCTATTACCGGGGAGCGCCGATGGAAAAGAAAGAGGGGCAGATCGTCT
GCGAATGCTTCGGTGTGACGGACCTGGAAATCGAAAAAGCCGTGAGGGAAAACAACCTGACTACCGTGGAGGATGTGACC
AACTATACCAAGGCGGGAGGCGGCTGTGGGAGCTGCCACGAAAAGATTCAGGAGATCATTGACCGGGTTCGGGCAGAAGT
TCGCGAACCCGTCCGTCCGAAACTGACGAACATCCAGAAAATCCGCATGATAGAGGATGTCCTTGAAAAGGAAATCCGAC
CATCCCTGAAAAACGACGGCGGTGATGTGGAACTCATCGATGTGGTGGGAAACCGGGTGCTGGTGGCGACCCGGGGGGCC
TGTGCCGTTTGCCGGGCCTCCCAGCAGACCCTGAAGGGTTTTGTGGAGTTCAAACTCCGTGAACTGGTAACACCAGAGCT
CATCGTTGAGGAGGTGAAGGCATGA

Upstream 100 bases:

>100_bases
GAAAGATTATGGCAAAAAGACGGGAAACATTGCGTAAAAAAGAAAAATTTTCAGACGCCTTTTGACGGCGTTGCATCATC
TCTGGAGAGGGGGAAGCTGC

Downstream 100 bases:

>100_bases
CGACGGTTTATCTGGATAACAATGCAACAACCCGTGTTGTGCCGGAGGTGCTGGAGGTGATGCTCCCCTATTTCAGCGAG
CTCTACGGCAATCCGTCAAG

Product: iscU protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MWEYTDKVKEHFLKPRNVGEVENPDGVAEVGSMACGDALRLTFKLDENKRIKEAKFKTFGCASAIAAASAMTEMIKGLTV
EEAEKITNQDIAAFLGGLPQEKMHCSVLGQQALEKAIAYYRGAPMEKKEGQIVCECFGVTDLEIEKAVRENNLTTVEDVT
NYTKAGGGCGSCHEKIQEIIDRVRAEVREPVRPKLTNIQKIRMIEDVLEKEIRPSLKNDGGDVELIDVVGNRVLVATRGA
CAVCRASQQTLKGFVEFKLRELVTPELIVEEVKA

Sequences:

>Translated_274_residues
MWEYTDKVKEHFLKPRNVGEVENPDGVAEVGSMACGDALRLTFKLDENKRIKEAKFKTFGCASAIAAASAMTEMIKGLTV
EEAEKITNQDIAAFLGGLPQEKMHCSVLGQQALEKAIAYYRGAPMEKKEGQIVCECFGVTDLEIEKAVRENNLTTVEDVT
NYTKAGGGCGSCHEKIQEIIDRVRAEVREPVRPKLTNIQKIRMIEDVLEKEIRPSLKNDGGDVELIDVVGNRVLVATRGA
CAVCRASQQTLKGFVEFKLRELVTPELIVEEVKA
>Mature_274_residues
MWEYTDKVKEHFLKPRNVGEVENPDGVAEVGSMACGDALRLTFKLDENKRIKEAKFKTFGCASAIAAASAMTEMIKGLTV
EEAEKITNQDIAAFLGGLPQEKMHCSVLGQQALEKAIAYYRGAPMEKKEGQIVCECFGVTDLEIEKAVRENNLTTVEDVT
NYTKAGGGCGSCHEKIQEIIDRVRAEVREPVRPKLTNIQKIRMIEDVLEKEIRPSLKNDGGDVELIDVVGNRVLVATRGA
CAVCRASQQTLKGFVEFKLRELVTPELIVEEVKA

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=121, Percent_Identity=47.9338842975207, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI24307953, Length=122, Percent_Identity=45.9016393442623, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1788878, Length=121, Percent_Identity=46.2809917355372, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543474, Length=122, Percent_Identity=47.5409836065574, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6325122, Length=123, Percent_Identity=47.1544715447154, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324800, Length=122, Percent_Identity=44.2622950819672, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355597, Length=121, Percent_Identity=48.7603305785124, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 30290; Mature: 30290

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWEYTDKVKEHFLKPRNVGEVENPDGVAEVGSMACGDALRLTFKLDENKRIKEAKFKTFG
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHH
CASAIAAASAMTEMIKGLTVEEAEKITNQDIAAFLGGLPQEKMHCSVLGQQALEKAIAYY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RGAPMEKKEGQIVCECFGVTDLEIEKAVRENNLTTVEDVTNYTKAGGGCGSCHEKIQEII
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
DRVRAEVREPVRPKLTNIQKIRMIEDVLEKEIRPSLKNDGGDVELIDVVGNRVLVATRGA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCH
CAVCRASQQTLKGFVEFKLRELVTPELIVEEVKA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MWEYTDKVKEHFLKPRNVGEVENPDGVAEVGSMACGDALRLTFKLDENKRIKEAKFKTFG
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHH
CASAIAAASAMTEMIKGLTVEEAEKITNQDIAAFLGGLPQEKMHCSVLGQQALEKAIAYY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RGAPMEKKEGQIVCECFGVTDLEIEKAVRENNLTTVEDVTNYTKAGGGCGSCHEKIQEII
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
DRVRAEVREPVRPKLTNIQKIRMIEDVLEKEIRPSLKNDGGDVELIDVVGNRVLVATRGA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCH
CAVCRASQQTLKGFVEFKLRELVTPELIVEEVKA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]