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The map label for this gene is ogt [H]

Identifier: 85858671

GI number: 85858671

Start: 845861

End: 847183

Strand: Direct

Name: ogt [H]

Synonym: SYN_02919

Alternate gene names: 85858671

Gene position: 845861-847183 (Clockwise)

Preceding gene: 85858670

Following gene: 85858672

Centisome position: 26.61

GC content: 55.33

Gene sequence:

>1323_bases
ATGATCATGGCCCATATTCGAGTTGATGAACTGATGAATCAGGAAGCAACTCATACCCCGGCGTTCTGTCTTCGCGACAC
ACCCTTTGGCCCTGTGGCCGTACTGTGGTCGGTTCATGGAGGCGAACCGAAGATCGGCCGCATCCTGCTGTCCGCTCCGG
AAATCTCAGCCGGGCAGGCTGTCCGGGCGCTTTTCCCTCATTCCAGGGGCGCTTCCTGTGAGGAGATCGGCCTGATCCTC
GATCAGATCGCGGCTTTTCTGGGCGGCGAGGATTCTCGATTTTCTCTCGACAGCCTTCGCCTTGATCTCTGTACCGCCTT
TCAAAAGAGAGTTCTGCTCGCCGATTATGCCATACCCCGGGGCCGGATCAGCACATACGGATTGATTGCGAAACACCTGG
ATAACCCGAAAGGAGCCCGAGCCGTGGGAACCGCCCTGGCGAACAATCCCTTCCCTCTTGTGGTTCCCTGTCACCGCGCG
ATCCGTTCCGATGGAAGTCTGGGAGGCTTCCAATACGGTCTGGAAATGAAAAGAAAATTGCTGGAGATGGAAGGGATTGT
CTTTCAGGATAAAAATCATGTCGCAACGCAGGATTTCTTCTATCGGGATGAAGCTGGAAGAGTCGGGGAAAAGGAGCATA
GCGGAAAACTGATCGTGAATGAGACGCTGAATGCGCAGCAGAGGCACTGGGAAAACACATTTTCTGAAGCAGAAGACCTG
TTCGGCATTGAACCCAGTGAGCCGGCGCGGATTGCCGTGGAGCTGTTTCTGAAAGAAGGAAAAAGGAGGATCCTCGAACT
GGGGGGAGGGCAGGGCCGGGATGCCCTGTTCTTTGCCAGCCAGGGGTTCCAGGTGGATGTCCTCGATTATTCCGAACAGT
CGATAACAACTATTGCGGAAAAGGCCCGGCAAATGGGTTTGTCCGACAAAGTGAAGGCCATCCGGCATGACATCCGGGAG
CCGCTTCCCTTTGAAGATGGGATTTTCGATGCCTGCTTTTCGCACATGCTCTACTGTATGGCGTTGACGACTGCTGAACT
GGAAAGACTGTCCAGCGAAGTGCGTCGGGTCCTGCGTCCCGCTGGGCTGAACATCTACACGGTTCGTCACACCGGCGATG
CGCATTACGGCGCCGGCATCTCCCGGGGCGAGGATCTGTATGAGGTCGGCGGTTTTATCGTCCATTTTTTCACCAGGGAA
AAGGTCGAACGACTGGCCGGAGGCTATGATCTGGTCGGCATCGACGAATTCGAAGAAGGCGACCTTCCCCGAAAACTGTT
CCGGGTGACCCTGAGAAAGAAGGATTGGGTTTCGGCCTCGTAA

Upstream 100 bases:

>100_bases
CGAGGTCTGCGGGATCCTGCTGCAACAGGAGAGCCCTCCTTCGTGAGCGGCTTTTCTTTGTCAATCTTCTGAATATGGAA
AGGGGCAGAAATGGAAAATA

Downstream 100 bases:

>100_bases
CTCCAACCGGTTGTTGTCCTGATGCCATATCCGAGCGCACTGTTCGTTGACAGCATGATCGGCCACTCGACGGTCGACAT
ATGCGCGCCTGCGTCGACTT

Product: 6-o-methylguanine DNA methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIMAHIRVDELMNQEATHTPAFCLRDTPFGPVAVLWSVHGGEPKIGRILLSAPEISAGQAVRALFPHSRGASCEEIGLIL
DQIAAFLGGEDSRFSLDSLRLDLCTAFQKRVLLADYAIPRGRISTYGLIAKHLDNPKGARAVGTALANNPFPLVVPCHRA
IRSDGSLGGFQYGLEMKRKLLEMEGIVFQDKNHVATQDFFYRDEAGRVGEKEHSGKLIVNETLNAQQRHWENTFSEAEDL
FGIEPSEPARIAVELFLKEGKRRILELGGGQGRDALFFASQGFQVDVLDYSEQSITTIAEKARQMGLSDKVKAIRHDIRE
PLPFEDGIFDACFSHMLYCMALTTAELERLSSEVRRVLRPAGLNIYTVRHTGDAHYGAGISRGEDLYEVGGFIVHFFTRE
KVERLAGGYDLVGIDEFEEGDLPRKLFRVTLRKKDWVSAS

Sequences:

>Translated_440_residues
MIMAHIRVDELMNQEATHTPAFCLRDTPFGPVAVLWSVHGGEPKIGRILLSAPEISAGQAVRALFPHSRGASCEEIGLIL
DQIAAFLGGEDSRFSLDSLRLDLCTAFQKRVLLADYAIPRGRISTYGLIAKHLDNPKGARAVGTALANNPFPLVVPCHRA
IRSDGSLGGFQYGLEMKRKLLEMEGIVFQDKNHVATQDFFYRDEAGRVGEKEHSGKLIVNETLNAQQRHWENTFSEAEDL
FGIEPSEPARIAVELFLKEGKRRILELGGGQGRDALFFASQGFQVDVLDYSEQSITTIAEKARQMGLSDKVKAIRHDIRE
PLPFEDGIFDACFSHMLYCMALTTAELERLSSEVRRVLRPAGLNIYTVRHTGDAHYGAGISRGEDLYEVGGFIVHFFTRE
KVERLAGGYDLVGIDEFEEGDLPRKLFRVTLRKKDWVSAS
>Mature_440_residues
MIMAHIRVDELMNQEATHTPAFCLRDTPFGPVAVLWSVHGGEPKIGRILLSAPEISAGQAVRALFPHSRGASCEEIGLIL
DQIAAFLGGEDSRFSLDSLRLDLCTAFQKRVLLADYAIPRGRISTYGLIAKHLDNPKGARAVGTALANNPFPLVVPCHRA
IRSDGSLGGFQYGLEMKRKLLEMEGIVFQDKNHVATQDFFYRDEAGRVGEKEHSGKLIVNETLNAQQRHWENTFSEAEDL
FGIEPSEPARIAVELFLKEGKRRILELGGGQGRDALFFASQGFQVDVLDYSEQSITTIAEKARQMGLSDKVKAIRHDIRE
PLPFEDGIFDACFSHMLYCMALTTAELERLSSEVRRVLRPAGLNIYTVRHTGDAHYGAGISRGEDLYEVGGFIVHFFTRE
KVERLAGGYDLVGIDEFEEGDLPRKLFRVTLRKKDWVSAS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=166, Percent_Identity=31.3253012048193, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787596, Length=84, Percent_Identity=41.6666666666667, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=44.4444444444444, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=44.4444444444444, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=80, Percent_Identity=38.75, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 49008; Mature: 49008

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMAHIRVDELMNQEATHTPAFCLRDTPFGPVAVLWSVHGGEPKIGRILLSAPEISAGQA
CEEEEEEHHHHHCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCH
VRALFPHSRGASCEEIGLILDQIAAFLGGEDSRFSLDSLRLDLCTAFQKRVLLADYAIPR
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCC
GRISTYGLIAKHLDNPKGARAVGTALANNPFPLVVPCHRAIRSDGSLGGFQYGLEMKRKL
CCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHH
LEMEGIVFQDKNHVATQDFFYRDEAGRVGEKEHSGKLIVNETLNAQQRHWENTFSEAEDL
HHHCCEEEECCCCCCCCHHEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
FGIEPSEPARIAVELFLKEGKRRILELGGGQGRDALFFASQGFQVDVLDYSEQSITTIAE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHH
KARQMGLSDKVKAIRHDIREPLPFEDGIFDACFSHMLYCMALTTAELERLSSEVRRVLRP
HHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGLNIYTVRHTGDAHYGAGISRGEDLYEVGGFIVHFFTREKVERLAGGYDLVGIDEFEEG
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
DLPRKLFRVTLRKKDWVSAS
CCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIMAHIRVDELMNQEATHTPAFCLRDTPFGPVAVLWSVHGGEPKIGRILLSAPEISAGQA
CEEEEEEHHHHHCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCH
VRALFPHSRGASCEEIGLILDQIAAFLGGEDSRFSLDSLRLDLCTAFQKRVLLADYAIPR
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCC
GRISTYGLIAKHLDNPKGARAVGTALANNPFPLVVPCHRAIRSDGSLGGFQYGLEMKRKL
CCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHH
LEMEGIVFQDKNHVATQDFFYRDEAGRVGEKEHSGKLIVNETLNAQQRHWENTFSEAEDL
HHHCCEEEECCCCCCCCHHEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
FGIEPSEPARIAVELFLKEGKRRILELGGGQGRDALFFASQGFQVDVLDYSEQSITTIAE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHH
KARQMGLSDKVKAIRHDIREPLPFEDGIFDACFSHMLYCMALTTAELERLSSEVRRVLRP
HHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGLNIYTVRHTGDAHYGAGISRGEDLYEVGGFIVHFFTREKVERLAGGYDLVGIDEFEEG
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
DLPRKLFRVTLRKKDWVSAS
CCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]