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The map label for this gene is pcrA [H]

Identifier: 85858661

GI number: 85858661

Start: 832274

End: 834094

Strand: Direct

Name: pcrA [H]

Synonym: SYN_02908

Alternate gene names: 85858661

Gene position: 832274-834094 (Clockwise)

Preceding gene: 85858660

Following gene: 85858662

Centisome position: 26.18

GC content: 50.85

Gene sequence:

>1821_bases
ATGCAACTAACTGAGAAACAGACAAAGATCATGGACGCCGAAGGGCACGTCCTCGTCACGGGAGGACCTGGCTCCGGTAA
GACTACTATTTCTATTCTCAAGGCAGCGCAAATCGCTGGATGCGGTCTACGTCCAGATCAGAAAATTCTGTTTCTCAGTT
TTGCCCGTGCGACCGTTTCAAGGGTTGTTGAAGCCATTGAATATGAGCAACAGATTCCGCACGAGCAGAAGAAACGGATC
GATGTCGAGACGTATCATTCGTTTTTTTGGCGTATTCTCAAAGCGCATGGTTATCTAGTCGGCTTACCGAGGCAGCTCTC
CATCCTCACACCACCAAGCGAAGCTATAGAGCTTTCGACGATTCGGAGTGGTTATGCGCCAGCGTCCAAACTGAATAATG
CTGAGAAAGCCGAGAAGCGTGCAAGGGAAGACGCGGAACGTCTGAGACTCGCACGGATTGAGGGTTGCGTCTGCTTTGAC
CTTTTCGCGCAGTATACTGGGGATATCCTGCAGGGGAGCAAGCGCATCCGTAAGCTGATCGCCACCATGTATCCAGTCAT
CATCCTTGACGAATTTCAGGACACAAATGTCGAGCAGTGGCGCGTCGTGCAGGCGCTTGGTGAGCACAGCATATTACTCG
CCCTCGCTGATCCTGAGCAGCGCATATATGACTGGATCGGGGCTGACCCTAAACGCTTAAGCCACTTCAAAGACGCATTC
GCGCCAACCGAGGTGGACCTCAGCACTGATAATCACCGCAGTGCCGGCATCGATATCGTTGCGTTCGGCAATGACATTCT
TACGGGCAAGTTCCGGGAAGAGCCCTACAGGGGAGTGCAAATCGATGTCTATACTCCAAATTTCAATCAGGCTTTTTCTG
CTCTAGTTACTACCACTTACAAAGCCAGAAAACGTCTCATCAATAGTGGTCGAGAGGATTGTTCTTTAGCCATCCTCGTG
CCTACGAAAAAGATGACTCGCCTTGTCTCGGATAGCTTTCGGTCACCCCTTGCAGGTATGACCAAGATTCCTCATGCGGC
CGCCATCGAGACGGAGGCCGCCATCTTGGGAGCCGAGATAATCGCCTTCCTGATGCAGCCCGATATGGACGGCAGCCACT
TCAAGCACTTTATCGACCTGTTGCGCAACTATTTTCATGGTAAGGGGGGCGACAACCCAACAAAGGCCGACCTCAAGGAG
GCCGAGAATATCCAGAAGGCATATAATGACTGGCGCTCCCGGCAAGCGGCCGGAAAGGACATCAACAAGAGAAGCATCCT
TATCGCCATTATCGCTGTTTATGGTACGACGCGCAAGATAGTGCTCACAGGGGATCCTGACAAAGATTGGCGTGCAGTTA
GGAACATTTTGGAAGAGGGCGCCTGCCCTCGTCTTAAAAGAGTTGCGGAAGAAGTCCGAAATATCCGCCTTCTGGAACGC
GGCACACAGTTAAGACAAGAGCTTTCACAAGACTGGCGCGACAAGGGTGCCTATGACAATGCTCTCAGGATCACCCAACA
GGCTTTTGTACGGGAACATTTTTCCACTAACGCCAATCCTGAATCTGGTGTTGTGGTAATGAACATGCACAAGGCTAAGG
GCAAGCAGTTCGACGAGGTGATTATCTTTGAAGGTTGGCCGATTAAACGGAAGGGACAACCTCCATACAATGCCGACCGT
ATTGTTCGGTACAACTCACGAAAGCATGATAGCGACCAATCTCGTCAGAATCTTCGAGTTAGCATAACGAGAGGAAAGTT
TCATGCCACGATCTTAACTCCTAAGGGTGACCCTTGTATCCTTCTTATTGGTGAGGGTTGA

Upstream 100 bases:

>100_bases
TCCTTGAAAGCAATTTGCGAACCTGTCCACGTTGGTGATGCCGACGGCGGCGCGCTCAACGCACCCGAAGCTCCAGATTC
TGAACAGGGAGTAGACGTGG

Downstream 100 bases:

>100_bases
TAAGTGAAGCAAATTGTCTTGATTATTTTATGGCGAAAATGTTGTAACCTAAATAGCTAAAATCACAGTCACCTGGCAGC
TAAAGCCTGTGGCTTCCGCT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MQLTEKQTKIMDAEGHVLVTGGPGSGKTTISILKAAQIAGCGLRPDQKILFLSFARATVSRVVEAIEYEQQIPHEQKKRI
DVETYHSFFWRILKAHGYLVGLPRQLSILTPPSEAIELSTIRSGYAPASKLNNAEKAEKRAREDAERLRLARIEGCVCFD
LFAQYTGDILQGSKRIRKLIATMYPVIILDEFQDTNVEQWRVVQALGEHSILLALADPEQRIYDWIGADPKRLSHFKDAF
APTEVDLSTDNHRSAGIDIVAFGNDILTGKFREEPYRGVQIDVYTPNFNQAFSALVTTTYKARKRLINSGREDCSLAILV
PTKKMTRLVSDSFRSPLAGMTKIPHAAAIETEAAILGAEIIAFLMQPDMDGSHFKHFIDLLRNYFHGKGGDNPTKADLKE
AENIQKAYNDWRSRQAAGKDINKRSILIAIIAVYGTTRKIVLTGDPDKDWRAVRNILEEGACPRLKRVAEEVRNIRLLER
GTQLRQELSQDWRDKGAYDNALRITQQAFVREHFSTNANPESGVVVMNMHKAKGKQFDEVIIFEGWPIKRKGQPPYNADR
IVRYNSRKHDSDQSRQNLRVSITRGKFHATILTPKGDPCILLIGEG

Sequences:

>Translated_606_residues
MQLTEKQTKIMDAEGHVLVTGGPGSGKTTISILKAAQIAGCGLRPDQKILFLSFARATVSRVVEAIEYEQQIPHEQKKRI
DVETYHSFFWRILKAHGYLVGLPRQLSILTPPSEAIELSTIRSGYAPASKLNNAEKAEKRAREDAERLRLARIEGCVCFD
LFAQYTGDILQGSKRIRKLIATMYPVIILDEFQDTNVEQWRVVQALGEHSILLALADPEQRIYDWIGADPKRLSHFKDAF
APTEVDLSTDNHRSAGIDIVAFGNDILTGKFREEPYRGVQIDVYTPNFNQAFSALVTTTYKARKRLINSGREDCSLAILV
PTKKMTRLVSDSFRSPLAGMTKIPHAAAIETEAAILGAEIIAFLMQPDMDGSHFKHFIDLLRNYFHGKGGDNPTKADLKE
AENIQKAYNDWRSRQAAGKDINKRSILIAIIAVYGTTRKIVLTGDPDKDWRAVRNILEEGACPRLKRVAEEVRNIRLLER
GTQLRQELSQDWRDKGAYDNALRITQQAFVREHFSTNANPESGVVVMNMHKAKGKQFDEVIIFEGWPIKRKGQPPYNADR
IVRYNSRKHDSDQSRQNLRVSITRGKFHATILTPKGDPCILLIGEG
>Mature_606_residues
MQLTEKQTKIMDAEGHVLVTGGPGSGKTTISILKAAQIAGCGLRPDQKILFLSFARATVSRVVEAIEYEQQIPHEQKKRI
DVETYHSFFWRILKAHGYLVGLPRQLSILTPPSEAIELSTIRSGYAPASKLNNAEKAEKRAREDAERLRLARIEGCVCFD
LFAQYTGDILQGSKRIRKLIATMYPVIILDEFQDTNVEQWRVVQALGEHSILLALADPEQRIYDWIGADPKRLSHFKDAF
APTEVDLSTDNHRSAGIDIVAFGNDILTGKFREEPYRGVQIDVYTPNFNQAFSALVTTTYKARKRLINSGREDCSLAILV
PTKKMTRLVSDSFRSPLAGMTKIPHAAAIETEAAILGAEIIAFLMQPDMDGSHFKHFIDLLRNYFHGKGGDNPTKADLKE
AENIQKAYNDWRSRQAAGKDINKRSILIAIIAVYGTTRKIVLTGDPDKDWRAVRNILEEGACPRLKRVAEEVRNIRLLER
GTQLRQELSQDWRDKGAYDNALRITQQAFVREHFSTNANPESGVVVMNMHKAKGKQFDEVIIFEGWPIKRKGQPPYNADR
IVRYNSRKHDSDQSRQNLRVSITRGKFHATILTPKGDPCILLIGEG

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=278, Percent_Identity=24.8201438848921, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 68316; Mature: 68316

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTEKQTKIMDAEGHVLVTGGPGSGKTTISILKAAQIAGCGLRPDQKILFLSFARATVS
CCCCCCHHHEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHH
RVVEAIEYEQQIPHEQKKRIDVETYHSFFWRILKAHGYLVGLPRQLSILTPPSEAIELST
HHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCHHHEEHHH
IRSGYAPASKLNNAEKAEKRAREDAERLRLARIEGCVCFDLFAQYTGDILQGSKRIRKLI
HHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATMYPVIILDEFQDTNVEQWRVVQALGEHSILLALADPEQRIYDWIGADPKRLSHFKDAF
HHHCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHC
APTEVDLSTDNHRSAGIDIVAFGNDILTGKFREEPYRGVQIDVYTPNFNQAFSALVTTTY
CCCEEECCCCCCCCCCEEEEEECCCEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
KARKRLINSGREDCSLAILVPTKKMTRLVSDSFRSPLAGMTKIPHAAAIETEAAILGAEI
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
IAFLMQPDMDGSHFKHFIDLLRNYFHGKGGDNPTKADLKEAENIQKAYNDWRSRQAAGKD
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
INKRSILIAIIAVYGTTRKIVLTGDPDKDWRAVRNILEEGACPRLKRVAEEVRNIRLLER
CCCCHHEEEEEEHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GTQLRQELSQDWRDKGAYDNALRITQQAFVREHFSTNANPESGVVVMNMHKAKGKQFDEV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCEE
IIFEGWPIKRKGQPPYNADRIVRYNSRKHDSDQSRQNLRVSITRGKFHATILTPKGDPCI
EEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECEEEEEEECCCCCCEE
LLIGEG
EEEECC
>Mature Secondary Structure
MQLTEKQTKIMDAEGHVLVTGGPGSGKTTISILKAAQIAGCGLRPDQKILFLSFARATVS
CCCCCCHHHEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHH
RVVEAIEYEQQIPHEQKKRIDVETYHSFFWRILKAHGYLVGLPRQLSILTPPSEAIELST
HHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCHHHEEHHH
IRSGYAPASKLNNAEKAEKRAREDAERLRLARIEGCVCFDLFAQYTGDILQGSKRIRKLI
HHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATMYPVIILDEFQDTNVEQWRVVQALGEHSILLALADPEQRIYDWIGADPKRLSHFKDAF
HHHCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHC
APTEVDLSTDNHRSAGIDIVAFGNDILTGKFREEPYRGVQIDVYTPNFNQAFSALVTTTY
CCCEEECCCCCCCCCCEEEEEECCCEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
KARKRLINSGREDCSLAILVPTKKMTRLVSDSFRSPLAGMTKIPHAAAIETEAAILGAEI
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
IAFLMQPDMDGSHFKHFIDLLRNYFHGKGGDNPTKADLKEAENIQKAYNDWRSRQAAGKD
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
INKRSILIAIIAVYGTTRKIVLTGDPDKDWRAVRNILEEGACPRLKRVAEEVRNIRLLER
CCCCHHEEEEEEHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GTQLRQELSQDWRDKGAYDNALRITQQAFVREHFSTNANPESGVVVMNMHKAKGKQFDEV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCEE
IIFEGWPIKRKGQPPYNADRIVRYNSRKHDSDQSRQNLRVSITRGKFHATILTPKGDPCI
EEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECEEEEEEECCCCCCEE
LLIGEG
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]