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The map label for this gene is dus [H]

Identifier: 85858573

GI number: 85858573

Start: 731735

End: 732715

Strand: Direct

Name: dus [H]

Synonym: SYN_02436

Alternate gene names: 85858573

Gene position: 731735-732715 (Clockwise)

Preceding gene: 85858572

Following gene: 85858574

Centisome position: 23.02

GC content: 45.77

Gene sequence:

>981_bases
ATGATTGTCATCGGCTCTTTAATAATAAAAAATAATGTGATTCTGGCGCCCATGGCCGGAATTTCCAATCTGCCTTTCAG
GATGATGGCGAGGGAGGCTGGATGTGCTCTTGCCTTTACGGAAATGATCAGTGCCAATGGTCTCGTTCGTGGGATTGAAA
AGAGTCAGCAGTATATGAAGTCCTGCATCGATGATAAGCCTTTGGGGGTGCAGATATTCGGAAACGATCCGACCACCATG
GCTGACGCGGTGAGGATTGTCGATGATTACGGAGCCGACCTGATTGATTTGAATATGGGATGCCCGGTAAAAAAAGTTGT
AAAAAATGGCGCCGGAGCCGCTCTGCTGAAGGATCCTGGATTGGTATCACAAATTCTCGAATCTGTCAGAAAGGCGACTC
GAAAACCGTTGACCGTGAAAATCCGTTCCGGATGGAATCTCCAGAGCATCAATGCAGTCCAGATAGCGAGAATTGCGGAG
CAGCAGGGGATTGATGCTGTTTTTGTTCATGCCCGAACTGCAGTTCAGGGGTTTGCCGGCTCAGCGGATTGGAGAATAAT
TGAAGAGATTAAGAATTCAGTAGGGATTAAAGTGATTGGAAATGGCGATGTCAAGTCAGGCGCTGACGCGTGGCGTATGA
TTAAAATGACGGGTTGCGATGGCGTCATGATTGGGAGGGGGGCATTGGGAAATCCTTGGATATTTAATGAAGCTCTTAAT
TATCAGTCCACTGTGCAAACACATTCTGAGCCGGTTTTAAATGAACGTAAAGAAATGATCTTACGTCATCTGGAAAGAGA
GACAATTTTTCTGGGTCAAAAAGAGGCTGTCAAACGCTTCCGGAAACATCTTCTCTGGTATACAAAGGGGATAAGGGGGG
GGGCCTCTTTCCGATCAAGAGTAACAACAATCAGTGATCAGAATTTATTGCTTCGTGAGATCGAAGCGTTTTTTGATCAG
GAAGAAATACCTTACCAATAA

Upstream 100 bases:

>100_bases
ACAAGACACGCTGCCGAGGTTCCTCTTTTGAATTCAGCGGTAACCGCGCCTGGATGAGGAGTTGGAATGATCGGTAAAGC
GAAGGGTATGAAATAAATTT

Downstream 100 bases:

>100_bases
AGGAAAAGGTTCCTAAATAAGATCTTGACTTTTAGATCGTGTATTGTAATATTTTGCTGAAATATCTCGATGGGCTGTGA
AGTGTAGGGTCTGAAATCCC

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MIVIGSLIIKNNVILAPMAGISNLPFRMMAREAGCALAFTEMISANGLVRGIEKSQQYMKSCIDDKPLGVQIFGNDPTTM
ADAVRIVDDYGADLIDLNMGCPVKKVVKNGAGAALLKDPGLVSQILESVRKATRKPLTVKIRSGWNLQSINAVQIARIAE
QQGIDAVFVHARTAVQGFAGSADWRIIEEIKNSVGIKVIGNGDVKSGADAWRMIKMTGCDGVMIGRGALGNPWIFNEALN
YQSTVQTHSEPVLNERKEMILRHLERETIFLGQKEAVKRFRKHLLWYTKGIRGGASFRSRVTTISDQNLLLREIEAFFDQ
EEIPYQ

Sequences:

>Translated_326_residues
MIVIGSLIIKNNVILAPMAGISNLPFRMMAREAGCALAFTEMISANGLVRGIEKSQQYMKSCIDDKPLGVQIFGNDPTTM
ADAVRIVDDYGADLIDLNMGCPVKKVVKNGAGAALLKDPGLVSQILESVRKATRKPLTVKIRSGWNLQSINAVQIARIAE
QQGIDAVFVHARTAVQGFAGSADWRIIEEIKNSVGIKVIGNGDVKSGADAWRMIKMTGCDGVMIGRGALGNPWIFNEALN
YQSTVQTHSEPVLNERKEMILRHLERETIFLGQKEAVKRFRKHLLWYTKGIRGGASFRSRVTTISDQNLLLREIEAFFDQ
EEIPYQ
>Mature_326_residues
MIVIGSLIIKNNVILAPMAGISNLPFRMMAREAGCALAFTEMISANGLVRGIEKSQQYMKSCIDDKPLGVQIFGNDPTTM
ADAVRIVDDYGADLIDLNMGCPVKKVVKNGAGAALLKDPGLVSQILESVRKATRKPLTVKIRSGWNLQSINAVQIARIAE
QQGIDAVFVHARTAVQGFAGSADWRIIEEIKNSVGIKVIGNGDVKSGADAWRMIKMTGCDGVMIGRGALGNPWIFNEALN
YQSTVQTHSEPVLNERKEMILRHLERETIFLGQKEAVKRFRKHLLWYTKGIRGGASFRSRVTTISDQNLLLREIEAFFDQ
EEIPYQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=36.4444444444444, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI40807366, Length=252, Percent_Identity=32.5396825396825, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=33.4763948497854, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI8923374, Length=249, Percent_Identity=30.9236947791165, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1789660, Length=316, Percent_Identity=39.5569620253165, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=33.7662337662338, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693211, Length=291, Percent_Identity=26.1168384879725, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=294, Percent_Identity=30.952380952381, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=230, Percent_Identity=34.7826086956522, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=31.5315315315315, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17510279, Length=254, Percent_Identity=27.1653543307087, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=286, Percent_Identity=26.5734265734266, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=30.3030303030303, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=29.646017699115, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24585320, Length=259, Percent_Identity=32.046332046332, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24580595, Length=249, Percent_Identity=30.9236947791165, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19920448, Length=249, Percent_Identity=30.9236947791165, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19921524, Length=232, Percent_Identity=32.7586206896552, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=30.241935483871, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35987; Mature: 35987

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIGSLIIKNNVILAPMAGISNLPFRMMAREAGCALAFTEMISANGLVRGIEKSQQYMK
CEEEEEEEEECCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHH
SCIDDKPLGVQIFGNDPTTMADAVRIVDDYGADLIDLNMGCPVKKVVKNGAGAALLKDPG
HHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCEEEECCCC
LVSQILESVRKATRKPLTVKIRSGWNLQSINAVQIARIAEQQGIDAVFVHARTAVQGFAG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHCCC
SADWRIIEEIKNSVGIKVIGNGDVKSGADAWRMIKMTGCDGVMIGRGALGNPWIFNEALN
CCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCEEEECCCCCCCCEEEHHHCC
YQSTVQTHSEPVLNERKEMILRHLERETIFLGQKEAVKRFRKHLLWYTKGIRGGASFRSR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
VTTISDQNLLLREIEAFFDQEEIPYQ
HEEECCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIVIGSLIIKNNVILAPMAGISNLPFRMMAREAGCALAFTEMISANGLVRGIEKSQQYMK
CEEEEEEEEECCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHH
SCIDDKPLGVQIFGNDPTTMADAVRIVDDYGADLIDLNMGCPVKKVVKNGAGAALLKDPG
HHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCEEEECCCC
LVSQILESVRKATRKPLTVKIRSGWNLQSINAVQIARIAEQQGIDAVFVHARTAVQGFAG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHCCC
SADWRIIEEIKNSVGIKVIGNGDVKSGADAWRMIKMTGCDGVMIGRGALGNPWIFNEALN
CCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCEEEECCCCCCCCEEEHHHCC
YQSTVQTHSEPVLNERKEMILRHLERETIFLGQKEAVKRFRKHLLWYTKGIRGGASFRSR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
VTTISDQNLLLREIEAFFDQEEIPYQ
HEEECCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]