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The map label for this gene is speE1 [H]

Identifier: 85858549

GI number: 85858549

Start: 707160

End: 708284

Strand: Direct

Name: speE1 [H]

Synonym: SYN_01347

Alternate gene names: 85858549

Gene position: 707160-708284 (Clockwise)

Preceding gene: 85858548

Following gene: 85858550

Centisome position: 22.24

GC content: 48.71

Gene sequence:

>1125_bases
GTGTCACGAATGTGGCGTGGGAATCCGTTAAGAAATGAAGGGGCGGCCATGAAGCCCACGGGAAACCAAATCCTTGCAGA
ATTTTTTCGTTGTTCTGCAAACATATTGAATGATTCCGACGCGCTTGAAAGAATCCTGGCGGGAGGGATTGAAGCCTGCG
GTCTCGGCCTGGTCAGCTTGAACAGCCATTGCTATGATCCCATCGGCATTACCTCTATTGCCGTCATCAGTGAATCCCAC
ATTGCAATCCATACCTATCCGGAAGCCCATCACGCCTCGGTGGATATTTTTACCTGTTCCCGGGGTAACGAGAAGTCACA
GCATCTTTTGCATTATCTGGAAGAGAAACTGATGCCGGCAACCACACGGGTTGTGGAGGTATCCAGGGGAAATCCCCTCG
AGGTGTCCCGGACCAACTGGATTACCGATGATAACAGCGCAACCGGTTTTGATGTCCGGTATCGCGTTGATCGGGAACTC
TTTTCCGGGTTATCCAGGTATCAGGATATCAAGGTCATCGAGAATCAGGATTTCGGCCGGATGCTGTTTCTGGATAATGA
TCTGCAGATTGCTGAAAGGGATGCCTGGCTCTATAACGAGGCGATGGTAAGTCCAGTGCGTGGAAAACTGGATGATCTTT
CGTCTGTGGCGATTTTAGGCGGCGGGGATGGCGGTGTTCTTTATGAACTGTTGAAATTGGATCCCGGCAGTGTTTCTGTC
ATTGATATCGATGAAGAAGTCATTCGGGTGGCAAGGACCTACCTCGAAAGCATCTGTCATTCTGCCTTCGAAGACGAAAG
GGTAAACATTGTTCTTGCCGATGTGTTCGAATACCTGGACGGGAGCCATCAGTTCGATGCAATGATCTATGACTTGACCA
TGCATCCGGAGTCCTTTATCGTGATGGATCGTGAGGACTACCTGAATCAGCTCTTTATGAAAATGAAGGAAGATTTGAAA
AGCCAGGGAGTTGTTTCCCTGCAGTGTGCTTCGGAATATGATCAAAAAACGACAGCGCTTGTAGAAAAATTGCTGAACCG
GCATTTCTCTTCCGTAAAGATGGACAAAAAGCTGATCCCCTCCTACGGCAGCATGTGGATGTTTGCATCCTGCATCAAAA
CCTGA

Upstream 100 bases:

>100_bases
TATTGACAGAACATCCTCATTTTTATATAAGAATCACTGTCTGTGAGATCTTATTAGTTAAATATTTGAATTTCTTGATA
ATATTTTCATATAGACTTCC

Downstream 100 bases:

>100_bases
AACGATCAGGAACCCCGTCTGAGCTTTTATGAACCGGCAACAACTGGAAGGTCGTATAGACATAGCGCCACGGACGCCCG
GTGTTTATCTCATGAAGGAT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSRMWRGNPLRNEGAAMKPTGNQILAEFFRCSANILNDSDALERILAGGIEACGLGLVSLNSHCYDPIGITSIAVISESH
IAIHTYPEAHHASVDIFTCSRGNEKSQHLLHYLEEKLMPATTRVVEVSRGNPLEVSRTNWITDDNSATGFDVRYRVDREL
FSGLSRYQDIKVIENQDFGRMLFLDNDLQIAERDAWLYNEAMVSPVRGKLDDLSSVAILGGGDGGVLYELLKLDPGSVSV
IDIDEEVIRVARTYLESICHSAFEDERVNIVLADVFEYLDGSHQFDAMIYDLTMHPESFIVMDREDYLNQLFMKMKEDLK
SQGVVSLQCASEYDQKTTALVEKLLNRHFSSVKMDKKLIPSYGSMWMFASCIKT

Sequences:

>Translated_374_residues
MSRMWRGNPLRNEGAAMKPTGNQILAEFFRCSANILNDSDALERILAGGIEACGLGLVSLNSHCYDPIGITSIAVISESH
IAIHTYPEAHHASVDIFTCSRGNEKSQHLLHYLEEKLMPATTRVVEVSRGNPLEVSRTNWITDDNSATGFDVRYRVDREL
FSGLSRYQDIKVIENQDFGRMLFLDNDLQIAERDAWLYNEAMVSPVRGKLDDLSSVAILGGGDGGVLYELLKLDPGSVSV
IDIDEEVIRVARTYLESICHSAFEDERVNIVLADVFEYLDGSHQFDAMIYDLTMHPESFIVMDREDYLNQLFMKMKEDLK
SQGVVSLQCASEYDQKTTALVEKLLNRHFSSVKMDKKLIPSYGSMWMFASCIKT
>Mature_373_residues
SRMWRGNPLRNEGAAMKPTGNQILAEFFRCSANILNDSDALERILAGGIEACGLGLVSLNSHCYDPIGITSIAVISESHI
AIHTYPEAHHASVDIFTCSRGNEKSQHLLHYLEEKLMPATTRVVEVSRGNPLEVSRTNWITDDNSATGFDVRYRVDRELF
SGLSRYQDIKVIENQDFGRMLFLDNDLQIAERDAWLYNEAMVSPVRGKLDDLSSVAILGGGDGGVLYELLKLDPGSVSVI
DIDEEVIRVARTYLESICHSAFEDERVNIVLADVFEYLDGSHQFDAMIYDLTMHPESFIVMDREDYLNQLFMKMKEDLKS
QGVVSLQCASEYDQKTTALVEKLLNRHFSSVKMDKKLIPSYGSMWMFASCIKT

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=198, Percent_Identity=31.8181818181818, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI21264341, Length=296, Percent_Identity=28.0405405405405, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786312, Length=224, Percent_Identity=28.125, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI212645616, Length=247, Percent_Identity=29.9595141700405, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323175, Length=238, Percent_Identity=28.9915966386555, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325326, Length=205, Percent_Identity=30.2439024390244, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21358269, Length=235, Percent_Identity=30.6382978723404, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24663238, Length=235, Percent_Identity=30.6382978723404, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645443, Length=206, Percent_Identity=30.0970873786408, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 42130; Mature: 41999

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRMWRGNPLRNEGAAMKPTGNQILAEFFRCSANILNDSDALERILAGGIEACGLGLVSL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHCCCCEEC
NSHCYDPIGITSIAVISESHIAIHTYPEAHHASVDIFTCSRGNEKSQHLLHYLEEKLMPA
CCCCCCCCCCCEEEEEECCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
TTRVVEVSRGNPLEVSRTNWITDDNSATGFDVRYRVDRELFSGLSRYQDIKVIENQDFGR
HHEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHCCEEEEECCCCCE
MLFLDNDLQIAERDAWLYNEAMVSPVRGKLDDLSSVAILGGGDGGVLYELLKLDPGSVSV
EEEEECCCCCHHCCHHHHHHHHHCCCCCCHHCCCEEEEEECCCCHHHHHHHHCCCCCEEE
IDIDEEVIRVARTYLESICHSAFEDERVNIVLADVFEYLDGSHQFDAMIYDLTMHPESFI
EECCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCEEEEEEEECCCEEE
VMDREDYLNQLFMKMKEDLKSQGVVSLQCASEYDQKTTALVEKLLNRHFSSVKMDKKLIP
EECHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SYGSMWMFASCIKT
CCCHHHHHHHHHCC
>Mature Secondary Structure 
SRMWRGNPLRNEGAAMKPTGNQILAEFFRCSANILNDSDALERILAGGIEACGLGLVSL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHCCCCEEC
NSHCYDPIGITSIAVISESHIAIHTYPEAHHASVDIFTCSRGNEKSQHLLHYLEEKLMPA
CCCCCCCCCCCEEEEEECCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
TTRVVEVSRGNPLEVSRTNWITDDNSATGFDVRYRVDRELFSGLSRYQDIKVIENQDFGR
HHEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHCCEEEEECCCCCE
MLFLDNDLQIAERDAWLYNEAMVSPVRGKLDDLSSVAILGGGDGGVLYELLKLDPGSVSV
EEEEECCCCCHHCCHHHHHHHHHCCCCCCHHCCCEEEEEECCCCHHHHHHHHCCCCCEEE
IDIDEEVIRVARTYLESICHSAFEDERVNIVLADVFEYLDGSHQFDAMIYDLTMHPESFI
EECCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCEEEEEEEECCCEEE
VMDREDYLNQLFMKMKEDLKSQGVVSLQCASEYDQKTTALVEKLLNRHFSSVKMDKKLIP
EECHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SYGSMWMFASCIKT
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]