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The map label for this gene is yngI [H]

Identifier: 85858542

GI number: 85858542

Start: 702655

End: 704334

Strand: Direct

Name: yngI [H]

Synonym: SYN_03128

Alternate gene names: 85858542

Gene position: 702655-704334 (Clockwise)

Preceding gene: 85858541

Following gene: 85858543

Centisome position: 22.1

GC content: 51.43

Gene sequence:

>1680_bases
TTGTTATTCTTAAAAAAAGGAGGAATACAAATGGGTAAAGCGGTAGATTATACAGTTTTGAAATCTGTCTGGCAGACAGA
GCATACCGTTACAAGGCAGCTTTATCGTATGTTGCAGAAATGGCCGGATAAAGTTGCGATCATTGATCCTTTGATGAAGA
AATCGCTGACCTACAGGCAGTGGGATGAAGAAGCCAACCAGTTCGCGCATGCCCTTCTTGAAGCAGGGTGCACCACTTAT
GATACCGTCATGAGTGATGTATTCAATTGTTCCGAGTGGTTTACTCTGTACATGGGATGTGCGAAGGCCAGGTGCGTAAA
TGCAATGCAGAATTTCATGCTTCCCGAGGGGCAGGTTTCTAAGCTGATGGATGACAGCGAAACGGCCGTTTTCGTCTATG
ACTCTTCCCTCAAGGATATGGCGGTGAAAGCCGTTGAACTGTGCAAGTTCAAGCCGAAGGTCTGCGTCATGATAGGTGAG
GGTGAAGTTCCCCCCGGCCATGTGAGTTATGCGAAATTTATCGAAGGCAAACCAAAGACTGCGCCGCCGACGGAGAAGGA
CGTCGAGATGTGGGATCCGTGCATGCTTATCTACACGTCCGGGACGACAGGCCTGCCCAAGGGATTTTTCGTGAGTCATG
CGACCATCTTCTTCGACTGCATGATGAATGGTCACCTGCACAAGGTTGACGAACACTGCGTCAGTCTTGCCACAAACCCG
CTCTTTCACAGAGGGGGAAATACAACGGGGGTTCTGCCCGTACTCCATCAAGGGGGAGCCGTTGTCATCATGAGATCCTT
TGACGAGAATCTGGCATTGGATTACATCGAAAAATACAAGGTTACCCACATGGTCAGCGCTCCTGTCATCTATGAGAGAA
TGTGTCATACGCAGGAAGAGAAAGCTCGCGATGTCAGCAGTCTGCGTGCCCTGTGCTCCATGGGCGCACCCCTCGATAAG
GATTCCTGTCTGAGAGTCATGAGAATCCTGTGTCCCGGCGTTTACAACGGTTATGGAACCGCGGATCAGCATTGGGTCAC
CATGCTGAAACCCTGGGAGTTGCCGGAAAAGGCGGGAACCATCGGCCTGCCGATTACGGAAGACATGATCGTACTCGTCC
GCCTTGATCTTGGACGTCGCGGCAATCCGAACAATCCTGAAGACCTCGTCCCGAGGGATGGTGTGACGGAAGGCGAAATC
GCTCTCAGAACCATGCATGGCTGTTATGGATATCTGAACAGACCCCAGGATACCGAGAAGGGATTCCCCTATCCGGGCTG
GCAGCTTCCGGGCGATACGGCGGTCTGGGACGCAGAAGGTTATATCTCCATCCGTGGCCGTACGGATGACATGATCATCA
CGGGTGCGGAAAATGTTCATCCCGTCGTGGTTGAGGAGGCGATCAAGGACCATCCCGGCGTCATCGACGTCTTCGTAACG
GGTGCCCCGAGCAAGAGGTGGGGTGAGGCGATCGTTGCCTATATCGCCCTGAAAGATCCCAATCTCAAGGAAGAAGACTT
TGTGGAATTCTGCAAGAATCACCCGAGGCTGGCAAGGTATCAGCGGCCAAAGTATTACAAGTTCGTTGACTTCAAGGAAC
TGCCCTTCAATCCATCAGGAAAGAAAATGCATTTTGTCATCAAGGAAAGGGCCAAGAAAGATTTCCCCGACTTGGATTAG

Upstream 100 bases:

>100_bases
TCTCAAACACAATGTTTTATCATTTGAAATGCAAGGAGGTGAGTGCGCAGGGTCCAGACTAAGTCACTTGAATCGATAAT
GAAAATCTTAATCGTTTTCA

Downstream 100 bases:

>100_bases
AAAGCATCCTTTTGAAAACATTGATAGAGTTTCCCGGAGAGAAAACCGGGAAACTCTATGATGAATGGCCGATTATGGGG
ATCTTTACTGAAGTCTTTGA

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MLFLKKGGIQMGKAVDYTVLKSVWQTEHTVTRQLYRMLQKWPDKVAIIDPLMKKSLTYRQWDEEANQFAHALLEAGCTTY
DTVMSDVFNCSEWFTLYMGCAKARCVNAMQNFMLPEGQVSKLMDDSETAVFVYDSSLKDMAVKAVELCKFKPKVCVMIGE
GEVPPGHVSYAKFIEGKPKTAPPTEKDVEMWDPCMLIYTSGTTGLPKGFFVSHATIFFDCMMNGHLHKVDEHCVSLATNP
LFHRGGNTTGVLPVLHQGGAVVIMRSFDENLALDYIEKYKVTHMVSAPVIYERMCHTQEEKARDVSSLRALCSMGAPLDK
DSCLRVMRILCPGVYNGYGTADQHWVTMLKPWELPEKAGTIGLPITEDMIVLVRLDLGRRGNPNNPEDLVPRDGVTEGEI
ALRTMHGCYGYLNRPQDTEKGFPYPGWQLPGDTAVWDAEGYISIRGRTDDMIITGAENVHPVVVEEAIKDHPGVIDVFVT
GAPSKRWGEAIVAYIALKDPNLKEEDFVEFCKNHPRLARYQRPKYYKFVDFKELPFNPSGKKMHFVIKERAKKDFPDLD

Sequences:

>Translated_559_residues
MLFLKKGGIQMGKAVDYTVLKSVWQTEHTVTRQLYRMLQKWPDKVAIIDPLMKKSLTYRQWDEEANQFAHALLEAGCTTY
DTVMSDVFNCSEWFTLYMGCAKARCVNAMQNFMLPEGQVSKLMDDSETAVFVYDSSLKDMAVKAVELCKFKPKVCVMIGE
GEVPPGHVSYAKFIEGKPKTAPPTEKDVEMWDPCMLIYTSGTTGLPKGFFVSHATIFFDCMMNGHLHKVDEHCVSLATNP
LFHRGGNTTGVLPVLHQGGAVVIMRSFDENLALDYIEKYKVTHMVSAPVIYERMCHTQEEKARDVSSLRALCSMGAPLDK
DSCLRVMRILCPGVYNGYGTADQHWVTMLKPWELPEKAGTIGLPITEDMIVLVRLDLGRRGNPNNPEDLVPRDGVTEGEI
ALRTMHGCYGYLNRPQDTEKGFPYPGWQLPGDTAVWDAEGYISIRGRTDDMIITGAENVHPVVVEEAIKDHPGVIDVFVT
GAPSKRWGEAIVAYIALKDPNLKEEDFVEFCKNHPRLARYQRPKYYKFVDFKELPFNPSGKKMHFVIKERAKKDFPDLD
>Mature_559_residues
MLFLKKGGIQMGKAVDYTVLKSVWQTEHTVTRQLYRMLQKWPDKVAIIDPLMKKSLTYRQWDEEANQFAHALLEAGCTTY
DTVMSDVFNCSEWFTLYMGCAKARCVNAMQNFMLPEGQVSKLMDDSETAVFVYDSSLKDMAVKAVELCKFKPKVCVMIGE
GEVPPGHVSYAKFIEGKPKTAPPTEKDVEMWDPCMLIYTSGTTGLPKGFFVSHATIFFDCMMNGHLHKVDEHCVSLATNP
LFHRGGNTTGVLPVLHQGGAVVIMRSFDENLALDYIEKYKVTHMVSAPVIYERMCHTQEEKARDVSSLRALCSMGAPLDK
DSCLRVMRILCPGVYNGYGTADQHWVTMLKPWELPEKAGTIGLPITEDMIVLVRLDLGRRGNPNNPEDLVPRDGVTEGEI
ALRTMHGCYGYLNRPQDTEKGFPYPGWQLPGDTAVWDAEGYISIRGRTDDMIITGAENVHPVVVEEAIKDHPGVIDVFVT
GAPSKRWGEAIVAYIALKDPNLKEEDFVEFCKNHPRLARYQRPKYYKFVDFKELPFNPSGKKMHFVIKERAKKDFPDLD

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=561, Percent_Identity=25.668449197861, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI187761345, Length=394, Percent_Identity=27.9187817258883, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI187761343, Length=394, Percent_Identity=27.9187817258883, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI40807357, Length=538, Percent_Identity=24.7211895910781, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI58082049, Length=523, Percent_Identity=22.3709369024857, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI8923896, Length=588, Percent_Identity=22.4489795918367, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI253970489, Length=594, Percent_Identity=22.7272727272727, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI38505220, Length=513, Percent_Identity=23.7816764132554, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI122937307, Length=384, Percent_Identity=27.0833333333333, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI42544132, Length=372, Percent_Identity=24.1935483870968, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI38524616, Length=267, Percent_Identity=26.9662921348315, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI115511026, Length=476, Percent_Identity=25.2100840336134, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1788107, Length=543, Percent_Identity=25.2302025782689, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI145693145, Length=524, Percent_Identity=25.9541984732824, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI221142682, Length=510, Percent_Identity=23.5294117647059, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786529, Length=560, Percent_Identity=24.4642857142857, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786810, Length=517, Percent_Identity=23.21083172147, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790505, Length=575, Percent_Identity=23.1304347826087, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=558, Percent_Identity=26.7025089605735, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=558, Percent_Identity=26.7025089605735, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=558, Percent_Identity=26.5232974910394, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17558820, Length=526, Percent_Identity=26.2357414448669, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=565, Percent_Identity=25.8407079646018, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=530, Percent_Identity=25.8490566037736, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=553, Percent_Identity=24.2314647377938, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=520, Percent_Identity=24.2307692307692, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=539, Percent_Identity=25.6029684601113, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=538, Percent_Identity=25.8364312267658, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17551278, Length=265, Percent_Identity=23.3962264150943, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115534440, Length=275, Percent_Identity=24.7272727272727, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=357, Percent_Identity=21.2885154061625, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=540, Percent_Identity=23.1481481481481, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323182, Length=417, Percent_Identity=26.378896882494, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=565, Percent_Identity=24.070796460177, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859661, Length=536, Percent_Identity=24.6268656716418, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24581924, Length=562, Percent_Identity=22.7758007117438, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17933690, Length=487, Percent_Identity=24.2299794661191, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355181, Length=541, Percent_Identity=22.181146025878, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648260, Length=359, Percent_Identity=23.6768802228412, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922652, Length=543, Percent_Identity=22.6519337016575, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364798, Length=549, Percent_Identity=22.2222222222222, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358303, Length=318, Percent_Identity=22.6415094339623, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130357, Length=372, Percent_Identity=23.1182795698925, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653035, Length=541, Percent_Identity=19.0388170055453, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356441, Length=349, Percent_Identity=22.0630372492837, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583465, Length=267, Percent_Identity=24.7191011235955, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583463, Length=267, Percent_Identity=24.7191011235955, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648257, Length=503, Percent_Identity=22.2664015904573, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 63125; Mature: 63125

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLKKGGIQMGKAVDYTVLKSVWQTEHTVTRQLYRMLQKWPDKVAIIDPLMKKSLTYRQ
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCHHH
WDEEANQFAHALLEAGCTTYDTVMSDVFNCSEWFTLYMGCAKARCVNAMQNFMLPEGQVS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KLMDDSETAVFVYDSSLKDMAVKAVELCKFKPKVCVMIGEGEVPPGHVSYAKFIEGKPKT
HHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCC
APPTEKDVEMWDPCMLIYTSGTTGLPKGFFVSHATIFFDCMMNGHLHKVDEHCVSLATNP
CCCCCCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
LFHRGGNTTGVLPVLHQGGAVVIMRSFDENLALDYIEKYKVTHMVSAPVIYERMCHTQEE
CEECCCCCCCEEHEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCHHH
KARDVSSLRALCSMGAPLDKDSCLRVMRILCPGVYNGYGTADQHWVTMLKPWELPEKAGT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHCCE
IGLPITEDMIVLVRLDLGRRGNPNNPEDLVPRDGVTEGEIALRTMHGCYGYLNRPQDTEK
ECCCCCCCEEEEEEEECCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GFPYPGWQLPGDTAVWDAEGYISIRGRTDDMIITGAENVHPVVVEEAIKDHPGVIDVFVT
CCCCCCCCCCCCCEEECCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEE
GAPSKRWGEAIVAYIALKDPNLKEEDFVEFCKNHPRLARYQRPKYYKFVDFKELPFNPSG
CCCCHHHHHEEEEEEEECCCCCCHHHHHHHHHCCCCHHHCCCCCCEEEECCCCCCCCCCC
KKMHFVIKERAKKDFPDLD
CEEEEEEHHHHHCCCCCCC
>Mature Secondary Structure
MLFLKKGGIQMGKAVDYTVLKSVWQTEHTVTRQLYRMLQKWPDKVAIIDPLMKKSLTYRQ
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCHHH
WDEEANQFAHALLEAGCTTYDTVMSDVFNCSEWFTLYMGCAKARCVNAMQNFMLPEGQVS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KLMDDSETAVFVYDSSLKDMAVKAVELCKFKPKVCVMIGEGEVPPGHVSYAKFIEGKPKT
HHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCC
APPTEKDVEMWDPCMLIYTSGTTGLPKGFFVSHATIFFDCMMNGHLHKVDEHCVSLATNP
CCCCCCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
LFHRGGNTTGVLPVLHQGGAVVIMRSFDENLALDYIEKYKVTHMVSAPVIYERMCHTQEE
CEECCCCCCCEEHEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCHHH
KARDVSSLRALCSMGAPLDKDSCLRVMRILCPGVYNGYGTADQHWVTMLKPWELPEKAGT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHCCE
IGLPITEDMIVLVRLDLGRRGNPNNPEDLVPRDGVTEGEIALRTMHGCYGYLNRPQDTEK
ECCCCCCCEEEEEEEECCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GFPYPGWQLPGDTAVWDAEGYISIRGRTDDMIITGAENVHPVVVEEAIKDHPGVIDVFVT
CCCCCCCCCCCCCEEECCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEE
GAPSKRWGEAIVAYIALKDPNLKEEDFVEFCKNHPRLARYQRPKYYKFVDFKELPFNPSG
CCCCHHHHHEEEEEEEECCCCCCHHHHHHHHHCCCCHHHCCCCCCEEEECCCCCCCCCCC
KKMHFVIKERAKKDFPDLD
CEEEEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]