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The map label for this gene is murE [H]

Identifier: 85858520

GI number: 85858520

Start: 672556

End: 674085

Strand: Direct

Name: murE [H]

Synonym: SYN_01740

Alternate gene names: 85858520

Gene position: 672556-674085 (Clockwise)

Preceding gene: 85858519

Following gene: 85858521

Centisome position: 21.15

GC content: 51.96

Gene sequence:

>1530_bases
ATGAACCTTTCGATCTTGTTGAAGGGAATCAACATTCTGGGAATTTCCGGACCTTCAGGCAGTGAAGTGAACGTGAGTTC
CGTATGCTACAATTCCCGGCAGTGTACTCCCGATTCCCTGTTCGTGGCGATAAGGGGTCTTAAATCCGACGGACATGAAT
ATATCGATGATGCGGTCCGTTGTGGTGCAAAAGTGATTGTCTGTGAAAAAGAAGTGACAATGCCTCCTGAAATTACAGTG
GTCAGAGTGGAAAACGGACGGAGAACCCTGGGGCTTCTGGCGAGAAATTTCTATGCGGATCCTTCCAGATGCCTGGTTGC
CGTTGCGGTGATGGGAACGAACGGGAAAACCACGGTCACCTATATCCTGGAGTCAATTTTTCAGGCGGCCGGCTTTTCGT
GCGGCGTCCTTGGTACTGTTAATTACCGTTATCCCGGCCTCGTCATGGAGGCACCCAATACAACACCGGAATCGCTGGAT
TTACAGCGGATTATTCGGGAAATGGTCGACCATGGGGTAACGCATCTCGTTGCCGAGGTATCGTCCCATGCCGTCGACCT
CCGACGCATCGATGATTGTGATTTTGATCTGGGAATTTTCACTAATCTTACCCAGGATCACCTGGATTATCACAAAACCA
TGGAGAATTATTTCCAGGCGAAAAAGAGGTTTTTTTCGGAAGTGCTTCCGGGCGGTAGAAAACAGCATCGTTACGCCATG
GCCGTGAATGCCGACGATCCATGGGGAAAACGCCTGATTCGAGAAACGGGGGAGATTTCCCGGCCTCTGACATTTGGAAT
TGAAGAGAAAGCCGACATCCGGGCGTCATCCTTTGATCTCTCTCTGGATGGCATTCGGGCGGAGATTGTGGTCGGCTCTA
TGAGCTTCGATGTCGACACCGCGCTGATTGGCAAGTTCAATCTGTACAATGTGCTGGCCGCGGTTTCGGCATCGGTGGCC
CTGCGCGTGCCGATTGAGGCCATTCGGCGGGGAGTGCGTGCAATGAATGCCGTTCCCGGACGTCTCGAGAAGGTCAGCGG
CTCGACACAGCCGGATGTATTTGTTGATTACGCCCACACTGACGACGCCCTGCGCAGGGTTCTTCAGAGTCTTTCGCCTT
TCAAGCGGGGAAGGATCATTACGGTGTTTGGCTGCGGTGGCGATCGCGACCGGGGAAAGAGACCCCTTATGGGTAAGGTG
GCGACAAATTACAGTGATCTGACAATTCTGACCTCTGATAATCCGAGATCGGAAGATCCCTTGTCCATTATTCGGGATAT
TGAAGTGGGCATTAACAGCCAATCCATTCCTGAAGGCTCTTCGGAGGATCTGTCCCGGGGAATGACCGGCAGAGGATATG
TGGTCATTCCGGACCGGAGGGAGGCCATCGGAGCGGCAATCCGGTCGGCACGTCCCTCCGATATTGTTCTCATTGCGGGA
AAAGGACATGAAGACTATCAGATCATTGGAGAGAAGCGTCTTTTCTTTGATGACCGCGCAGTTGCAAAAGAATTCCTTCA
GAATACATAA

Upstream 100 bases:

>100_bases
ATGGGCTGTTAAACAGCATCCGGTACCCGGCACACCGATAGCGCATTCAACCCATTGTTCCGTGATCTTCGATCACGGGA
ATTAAAAAGGCCTTTATGAA

Downstream 100 bases:

>100_bases
AAAAAACAGGCTGAAAGGGGCAAACCGATTTCCAGGTGATCGGCCTTATTCCAATTCCATATCACAGCGCATTCTTTGCC
GGCTTTGCTATCGTTTCCGT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNLSILLKGINILGISGPSGSEVNVSSVCYNSRQCTPDSLFVAIRGLKSDGHEYIDDAVRCGAKVIVCEKEVTMPPEITV
VRVENGRRTLGLLARNFYADPSRCLVAVAVMGTNGKTTVTYILESIFQAAGFSCGVLGTVNYRYPGLVMEAPNTTPESLD
LQRIIREMVDHGVTHLVAEVSSHAVDLRRIDDCDFDLGIFTNLTQDHLDYHKTMENYFQAKKRFFSEVLPGGRKQHRYAM
AVNADDPWGKRLIRETGEISRPLTFGIEEKADIRASSFDLSLDGIRAEIVVGSMSFDVDTALIGKFNLYNVLAAVSASVA
LRVPIEAIRRGVRAMNAVPGRLEKVSGSTQPDVFVDYAHTDDALRRVLQSLSPFKRGRIITVFGCGGDRDRGKRPLMGKV
ATNYSDLTILTSDNPRSEDPLSIIRDIEVGINSQSIPEGSSEDLSRGMTGRGYVVIPDRREAIGAAIRSARPSDIVLIAG
KGHEDYQIIGEKRLFFDDRAVAKEFLQNT

Sequences:

>Translated_509_residues
MNLSILLKGINILGISGPSGSEVNVSSVCYNSRQCTPDSLFVAIRGLKSDGHEYIDDAVRCGAKVIVCEKEVTMPPEITV
VRVENGRRTLGLLARNFYADPSRCLVAVAVMGTNGKTTVTYILESIFQAAGFSCGVLGTVNYRYPGLVMEAPNTTPESLD
LQRIIREMVDHGVTHLVAEVSSHAVDLRRIDDCDFDLGIFTNLTQDHLDYHKTMENYFQAKKRFFSEVLPGGRKQHRYAM
AVNADDPWGKRLIRETGEISRPLTFGIEEKADIRASSFDLSLDGIRAEIVVGSMSFDVDTALIGKFNLYNVLAAVSASVA
LRVPIEAIRRGVRAMNAVPGRLEKVSGSTQPDVFVDYAHTDDALRRVLQSLSPFKRGRIITVFGCGGDRDRGKRPLMGKV
ATNYSDLTILTSDNPRSEDPLSIIRDIEVGINSQSIPEGSSEDLSRGMTGRGYVVIPDRREAIGAAIRSARPSDIVLIAG
KGHEDYQIIGEKRLFFDDRAVAKEFLQNT
>Mature_509_residues
MNLSILLKGINILGISGPSGSEVNVSSVCYNSRQCTPDSLFVAIRGLKSDGHEYIDDAVRCGAKVIVCEKEVTMPPEITV
VRVENGRRTLGLLARNFYADPSRCLVAVAVMGTNGKTTVTYILESIFQAAGFSCGVLGTVNYRYPGLVMEAPNTTPESLD
LQRIIREMVDHGVTHLVAEVSSHAVDLRRIDDCDFDLGIFTNLTQDHLDYHKTMENYFQAKKRFFSEVLPGGRKQHRYAM
AVNADDPWGKRLIRETGEISRPLTFGIEEKADIRASSFDLSLDGIRAEIVVGSMSFDVDTALIGKFNLYNVLAAVSASVA
LRVPIEAIRRGVRAMNAVPGRLEKVSGSTQPDVFVDYAHTDDALRRVLQSLSPFKRGRIITVFGCGGDRDRGKRPLMGKV
ATNYSDLTILTSDNPRSEDPLSIIRDIEVGINSQSIPEGSSEDLSRGMTGRGYVVIPDRREAIGAAIRSARPSDIVLIAG
KGHEDYQIIGEKRLFFDDRAVAKEFLQNT

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=503, Percent_Identity=34.1948310139165, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1786274, Length=332, Percent_Identity=25.9036144578313, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 55939; Mature: 55939

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00485 A_DEAMINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSILLKGINILGISGPSGSEVNVSSVCYNSRQCTPDSLFVAIRGLKSDGHEYIDDAVR
CCCEEEEECCEEEEECCCCCCCEEHHHHEECCCCCCCHHEEEEEECCCCCCHHHHHHHHH
CGAKVIVCEKEVTMPPEITVVRVENGRRTLGLLARNFYADPSRCLVAVAVMGTNGKTTVT
CCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHH
YILESIFQAAGFSCGVLGTVNYRYPGLVMEAPNTTPESLDLQRIIREMVDHGVTHLVAEV
HHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SSHAVDLRRIDDCDFDLGIFTNLTQDHLDYHKTMENYFQAKKRFFSEVLPGGRKQHRYAM
HHHHEEHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
AVNADDPWGKRLIRETGEISRPLTFGIEEKADIRASSFDLSLDGIRAEIVVGSMSFDVDT
EECCCCHHHHHHHHHHCCCCCCEEECCCCCCCCEECCCCEEECCEEEEEEEEEECCCCCH
ALIGKFNLYNVLAAVSASVALRVPIEAIRRGVRAMNAVPGRLEKVSGSTQPDVFVDYAHT
HHHCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEECCC
DDALRRVLQSLSPFKRGRIITVFGCGGDRDRGKRPLMGKVATNYSDLTILTSDNPRSEDP
HHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCH
LSIIRDIEVGINSQSIPEGSSEDLSRGMTGRGYVVIPDRREAIGAAIRSARPSDIVLIAG
HHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEEEEC
KGHEDYQIIGEKRLFFDDRAVAKEFLQNT
CCCCCEEEECCEEEEECCHHHHHHHHHCC
>Mature Secondary Structure
MNLSILLKGINILGISGPSGSEVNVSSVCYNSRQCTPDSLFVAIRGLKSDGHEYIDDAVR
CCCEEEEECCEEEEECCCCCCCEEHHHHEECCCCCCCHHEEEEEECCCCCCHHHHHHHHH
CGAKVIVCEKEVTMPPEITVVRVENGRRTLGLLARNFYADPSRCLVAVAVMGTNGKTTVT
CCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHH
YILESIFQAAGFSCGVLGTVNYRYPGLVMEAPNTTPESLDLQRIIREMVDHGVTHLVAEV
HHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SSHAVDLRRIDDCDFDLGIFTNLTQDHLDYHKTMENYFQAKKRFFSEVLPGGRKQHRYAM
HHHHEEHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
AVNADDPWGKRLIRETGEISRPLTFGIEEKADIRASSFDLSLDGIRAEIVVGSMSFDVDT
EECCCCHHHHHHHHHHCCCCCCEEECCCCCCCCEECCCCEEECCEEEEEEEEEECCCCCH
ALIGKFNLYNVLAAVSASVALRVPIEAIRRGVRAMNAVPGRLEKVSGSTQPDVFVDYAHT
HHHCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEECCC
DDALRRVLQSLSPFKRGRIITVFGCGGDRDRGKRPLMGKVATNYSDLTILTSDNPRSEDP
HHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCH
LSIIRDIEVGINSQSIPEGSSEDLSRGMTGRGYVVIPDRREAIGAAIRSARPSDIVLIAG
HHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEEEEC
KGHEDYQIIGEKRLFFDDRAVAKEFLQNT
CCCCCEEEECCEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]