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The map label for this gene is noeL [H]

Identifier: 85375511

GI number: 85375511

Start: 2704892

End: 2705929

Strand: Direct

Name: noeL [H]

Synonym: ELI_13420

Alternate gene names: 85375511

Gene position: 2704892-2705929 (Clockwise)

Preceding gene: 85375507

Following gene: 85375514

Centisome position: 88.62

GC content: 62.04

Gene sequence:

>1038_bases
GTGACAGGACAGGATGGTGCCTATCTGGCCCGGCTTCTGCTGGAAAAGGGCTACGAAGTGCACGGCCTCAAGCGGCGCTC
GTCCAGCTTCAATACGGGCCGGATCGAAGACATCTATCAGGATCCTCACGAGCCCGATCCGCGACTGATCCTGCATTATG
GCGATATGACCGATGCGACCAACCTGATCCGCATCGTGCAGGAAAGCCGCCCGCATGAGATCTACAATCTGGCGGCGCAA
AGCCATGTGCAGGTGAGCTTCGAAACGCCCGAATATACTGCCAATGCCGATGCCATCGGCCCGCTCCGCCTGCTCGAAGC
GATCCGTATCCTCGGGCTGGAAGAGGAGACCCGCTTCTACCAGGCTTCGACATCGGAGCTCTACGGGTTGGTACAGGAAG
CACCGCAAAGCGAGACGACGCCGTTCTACCCGCGCAGCCCCTATGGTGTGGCCAAGCTCTACGGCTACTGGATCACGGTC
AATTATCGCGAGGCTTACGGAATGCATGCTTCCAACGGCATCCTGTTCAATCACGAAAGCCCGCTGCGCGGCGAAACCTT
CGTCACCCGCAAGATCACCCGCGCGGCGGCGGCCATTGCGCTGGGGCGGCAGGACAAGCTCTATCTCGGCAATCTCGACG
CGCAGCGGGACTGGGGCCACGCCCGCGAATATGCCGAGGGCATGTGGCGCATGCTGCAGCGGGATGAGCCCGACGATTAC
GTGTTGGCGACCGGCGTCACCACTTCGGTACGCGATTTCACGCGCTGGGCGTTCGAAGATGCCGGGATCGCGCTGGTTTT
TACCGGCGAAGGCGTCGACGAGAAGGGCGTGTGTGCCAAGACCGGCCGCGAACTGGTCGAAGTCGATCCGCGCTATTTCC
GGCCGGCGGAGGTCGAACTCCTGATCGGCGATGCCTCGAAGGCGCGGGACAGGTTGGGCTGGGAACCCAGGCTCGGCGTG
CGAGAATTGGCGCGGGAAATGGTGGCCGCAGACCTCGAAATCATGCGCGACGATACGATCGCCAGGGAAGATTGCTGA

Upstream 100 bases:

>100_bases
CTGTCGGTCGCGCGGCTAAGGGTGTCGCAGCATTGAGAAATTCCTGCTGCGGGAGGCTAGAGTGGCAAAACCAAACGGCA
AACGCGCTCTCGTCACCGGT

Downstream 100 bases:

>100_bases
TCAGCTCGGCTGCGTGATCAGCGCCTTGCCCCGCGTACAGGTGATATGCGCGATATCCTCGACCAGCGCGCACGATCCGG
CAGGCACGGCCTCGCCGGCG

Product: GDP-mannose 46-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTGQDGAYLARLLLEKGYEVHGLKRRSSSFNTGRIEDIYQDPHEPDPRLILHYGDMTDATNLIRIVQESRPHEIYNLAAQ
SHVQVSFETPEYTANADAIGPLRLLEAIRILGLEEETRFYQASTSELYGLVQEAPQSETTPFYPRSPYGVAKLYGYWITV
NYREAYGMHASNGILFNHESPLRGETFVTRKITRAAAAIALGRQDKLYLGNLDAQRDWGHAREYAEGMWRMLQRDEPDDY
VLATGVTTSVRDFTRWAFEDAGIALVFTGEGVDEKGVCAKTGRELVEVDPRYFRPAEVELLIGDASKARDRLGWEPRLGV
RELAREMVAADLEIMRDDTIAREDC

Sequences:

>Translated_345_residues
MTGQDGAYLARLLLEKGYEVHGLKRRSSSFNTGRIEDIYQDPHEPDPRLILHYGDMTDATNLIRIVQESRPHEIYNLAAQ
SHVQVSFETPEYTANADAIGPLRLLEAIRILGLEEETRFYQASTSELYGLVQEAPQSETTPFYPRSPYGVAKLYGYWITV
NYREAYGMHASNGILFNHESPLRGETFVTRKITRAAAAIALGRQDKLYLGNLDAQRDWGHAREYAEGMWRMLQRDEPDDY
VLATGVTTSVRDFTRWAFEDAGIALVFTGEGVDEKGVCAKTGRELVEVDPRYFRPAEVELLIGDASKARDRLGWEPRLGV
RELAREMVAADLEIMRDDTIAREDC
>Mature_344_residues
TGQDGAYLARLLLEKGYEVHGLKRRSSSFNTGRIEDIYQDPHEPDPRLILHYGDMTDATNLIRIVQESRPHEIYNLAAQS
HVQVSFETPEYTANADAIGPLRLLEAIRILGLEEETRFYQASTSELYGLVQEAPQSETTPFYPRSPYGVAKLYGYWITVN
YREAYGMHASNGILFNHESPLRGETFVTRKITRAAAAIALGRQDKLYLGNLDAQRDWGHAREYAEGMWRMLQRDEPDDYV
LATGVTTSVRDFTRWAFEDAGIALVFTGEGVDEKGVCAKTGRELVEVDPRYFRPAEVELLIGDASKARDRLGWEPRLGVR
ELAREMVAADLEIMRDDTIAREDC

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=339, Percent_Identity=59.5870206489675, Blast_Score=436, Evalue=1e-122,
Organism=Escherichia coli, GI1788366, Length=352, Percent_Identity=63.6363636363636, Blast_Score=476, Evalue=1e-136,
Organism=Escherichia coli, GI48994969, Length=184, Percent_Identity=29.3478260869565, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788353, Length=287, Percent_Identity=25.4355400696864, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=343, Percent_Identity=62.3906705539359, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17539424, Length=343, Percent_Identity=62.3906705539359, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17539422, Length=343, Percent_Identity=62.3906705539359, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901786, Length=343, Percent_Identity=62.3906705539359, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901788, Length=343, Percent_Identity=62.3906705539359, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17507723, Length=343, Percent_Identity=62.9737609329446, Blast_Score=446, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24158427, Length=342, Percent_Identity=59.3567251461988, Blast_Score=430, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39131; Mature: 39000

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQDGAYLARLLLEKGYEVHGLKRRSSSFNTGRIEDIYQDPHEPDPRLILHYGDMTDAT
CCCCCHHHHHHHHHHCCCCEECCHHCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCHH
NLIRIVQESRPHEIYNLAAQSHVQVSFETPEYTANADAIGPLRLLEAIRILGLEEETRFY
HHHHHHHCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH
QASTSELYGLVQEAPQSETTPFYPRSPYGVAKLYGYWITVNYREAYGMHASNGILFNHES
HCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEEEEEEEHHHCCCCCCCCEEECCCC
PLRGETFVTRKITRAAAAIALGRQDKLYLGNLDAQRDWGHAREYAEGMWRMLQRDEPDDY
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
VLATGVTTSVRDFTRWAFEDAGIALVFTGEGVDEKGVCAKTGRELVEVDPRYFRPAEVEL
EEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHEECCCCCCCCCEEEE
LIGDASKARDRLGWEPRLGVRELAREMVAADLEIMRDDTIAREDC
EECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TGQDGAYLARLLLEKGYEVHGLKRRSSSFNTGRIEDIYQDPHEPDPRLILHYGDMTDAT
CCCCHHHHHHHHHHCCCCEECCHHCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCHH
NLIRIVQESRPHEIYNLAAQSHVQVSFETPEYTANADAIGPLRLLEAIRILGLEEETRFY
HHHHHHHCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH
QASTSELYGLVQEAPQSETTPFYPRSPYGVAKLYGYWITVNYREAYGMHASNGILFNHES
HCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEEEEEEEHHHCCCCCCCCEEECCCC
PLRGETFVTRKITRAAAAIALGRQDKLYLGNLDAQRDWGHAREYAEGMWRMLQRDEPDDY
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
VLATGVTTSVRDFTRWAFEDAGIALVFTGEGVDEKGVCAKTGRELVEVDPRYFRPAEVEL
EEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHEECCCCCCCCCEEEE
LIGDASKARDRLGWEPRLGVRELAREMVAADLEIMRDDTIAREDC
EECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10065558 [H]