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The map label for this gene is rlmH

Identifier: 85375474

GI number: 85375474

Start: 2665381

End: 2665803

Strand: Direct

Name: rlmH

Synonym: ELI_13235

Alternate gene names: 85375474

Gene position: 2665381-2665803 (Clockwise)

Preceding gene: 85375473

Following gene: 85375475

Centisome position: 87.32

GC content: 65.72

Gene sequence:

>423_bases
ATGCTCCTCCACATCATCGCACGTGGCAAGATCGCCCGCTCGCCGGAGGAGGAGCTGGTCACGCGTTACGAGAAACGCCT
GACCTGGCCGGTGAAACTGACAGAATTGCCGGAAACCGGGGGACGCATCCCCGATCCGCAGACCCCATTCAAGACCGTGC
TGCTCGATGAGCGCGGCAAGGACCTGTCCTCCCATCGGTTGGCTAGGCAGCTGGAGCGCTGGCGCGATGACGGGATGCGC
GAAACGCGCTTCGTGCTGGGCGCGGCGGACGGGCATGCACAGGAGGAACGAGCGGAGGCCGACCTCTTGCTGGCCTTCGG
CTCGGCGACCTGGCCGCATCTGCTGGCGCGGGCGATGCTGATGGAACAGCTGTACCGGGCGACCAGCATCCTTGCAGGCC
ATCCCTATCATCGGGCAGGGTGA

Upstream 100 bases:

>100_bases
CAAGGGCGACCGCCCGCCCGCAGGGATGCGACCGAAGGTCGCGTGAGCGAGGATAGCCAAGGCTGCGGATGCAGCCGCCG
GCGCTTGAGGCCCAAACAAA

Downstream 100 bases:

>100_bases
CGGCATCATGATCTCGCGCCTGCTCCTGTTGCTGCTCGGACTGCTCGCCTTGGTGGGTGTCGCGGCGCTGGCGCCGGGCC
TTGTGGCGCAGGCGGATGGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MLLHIIARGKIARSPEEELVTRYEKRLTWPVKLTELPETGGRIPDPQTPFKTVLLDERGKDLSSHRLARQLERWRDDGMR
ETRFVLGAADGHAQEERAEADLLLAFGSATWPHLLARAMLMEQLYRATSILAGHPYHRAG

Sequences:

>Translated_140_residues
MLLHIIARGKIARSPEEELVTRYEKRLTWPVKLTELPETGGRIPDPQTPFKTVLLDERGKDLSSHRLARQLERWRDDGMR
ETRFVLGAADGHAQEERAEADLLLAFGSATWPHLLARAMLMEQLYRATSILAGHPYHRAG
>Mature_140_residues
MLLHIIARGKIARSPEEELVTRYEKRLTWPVKLTELPETGGRIPDPQTPFKTVLLDERGKDLSSHRLARQLERWRDDGMR
ETRFVLGAADGHAQEERAEADLLLAFGSATWPHLLARAMLMEQLYRATSILAGHPYHRAG

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=88, Percent_Identity=39.7727272727273, Blast_Score=66, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_ERYLH (Q2N6F2)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459536.1
- ProteinModelPortal:   Q2N6F2
- STRING:   Q2N6F2
- GeneID:   3869352
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_13235
- NMPDR:   fig|314225.3.peg.377
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   WPHLLAR
- PhylomeDB:   Q2N6F2
- ProtClustDB:   CLSK835479
- BioCyc:   ELIT314225:ELI_13235-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 15977; Mature: 15977

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHIIARGKIARSPEEELVTRYEKRLTWPVKLTELPETGGRIPDPQTPFKTVLLDERGK
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHEECCCCC
DLSSHRLARQLERWRDDGMRETRFVLGAADGHAQEERAEADLLLAFGSATWPHLLARAML
CHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHH
MEQLYRATSILAGHPYHRAG
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLLHIIARGKIARSPEEELVTRYEKRLTWPVKLTELPETGGRIPDPQTPFKTVLLDERGK
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHEECCCCC
DLSSHRLARQLERWRDDGMRETRFVLGAADGHAQEERAEADLLLAFGSATWPHLLARAML
CHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHH
MEQLYRATSILAGHPYHRAG
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA