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The map label for this gene is fumB [H]

Identifier: 85375408

GI number: 85375408

Start: 2602521

End: 2604047

Strand: Reverse

Name: fumB [H]

Synonym: ELI_12905

Alternate gene names: 85375408

Gene position: 2604047-2602521 (Counterclockwise)

Preceding gene: 85375414

Following gene: 85375407

Centisome position: 85.31

GC content: 63.72

Gene sequence:

>1527_bases
ATGAGCGACATGACCACGATACGCGAAGACGATCTCATCGAAAGCGTCGCCGACGCGCTGCAATATATCTCCTACTATCA
CCCGATGGATTACATCCGCGCCCTGGGCGATGCCTACGAGGCGGAGCAGGGCCCGGCGGCGAAGGATGCGATCGCACAAA
TCCTTACCAACAGCCGCATGTGCGCCGAGGGGCATCGACCGATCTGCCAGGACACCGGCATCGTCAACGTGTTCGTGAAA
TGGGGGCAGGACTGCCGGCTCGTCTCTAGGCGGAGCCTGCAGGATGTCGTCGATGAAGGCGTGCGCCGCGCCTACAATCA
TCCTGACAACAAGCTGCGCGCCTCGATCCTCGCCGATCCGGCGTTCACCCGCCGCAACACCCGCGACAACACGCCGTGTG
TGCTGTCGGTCGAGATGGTGCCGGGCCGCATGGTGGAGATCGACGTCGCGGCCAAGGGCGGCGGCAGCGAGAACAAGTCC
AAGTTCAAGATGATGAATCCCAGCGACAATATCGTCGACTGGGTGGTCGAACAGCTGCCCAGCATGGGCGCGGGCTGGTG
TCCGCCGGGCATGCTGGGCATCGGCATCGGTGGCACGGCAGAACATTGCATGCGGCTCGCCAAGCTGAGCCTGATGGAGC
CGATCGACATGGGCCAGCTCAAGCAGCGCGGGGCGCAGAACGATCTCGAGCAATTGCGGATCGACATTTTCGATGCGGTC
AACGCCCAGGGGATCGGGGCGCAGGGACTGGGCGGGCTGTCGACTGTGCTCGACGTGAAGATTCTCGACGCGCCCTGCCA
TGCGGCAGGTAAGCCTGTCGGGATGATCCCCAATTGCGCCGCCACGCGCCACGCCCATTTCACGCTCGATGGCTCCGGCC
CGGCCTACCTCGAACAGCCCGATCTCGACCAATGGCCCAAGGTCGACTGGCAGCCCGACAGCGCTGCAAAGCGCGTCGAT
CTCGACAACCTGACGCAGGAAGAGGTCAATAGCTGGGAGCATGGCGACCGGCTGCTGCTCTCGGGCAAGATGCTGACCGG
GCGCGATGCTGCCCACAAGCGCATCCAGGACATGCTCGACAAGGGCGAAGAGCTGCCGGTCGATTTCAAGGGCCGCGCGA
TTTATTACGTCGGCCCGGTCGATCCGGTGATGGGCGAAGTCGTCGGCCCCGCCGGCCCGACCACCGCTACGCGGATGGAC
AAATTCACCGAGATGATGCTCGACCTCGGCCTGCTGGCGATGATCGGCAAGGCCGAACGCGGCCATGACGCGGTGGAAGT
GATCAGCCGCTACAAGGTCGCCTACCTCATGGCGACGGGCGGCGCGGCTTACCTCGTGGCGCGCGCGATCAAGGAATCCA
GGGTCGTCGGCTTCGAAGACCTCGGAATGGAGGCGATCTACGAATTCACCGTCAAGGACATGCCGGTGACGGTGGCCGTC
GACAGCCAAGGAAACAACGTCCACACGCTTGCCCCTGCCAAGTGGAAGCAGCGGATAGCCGACGAAAAGCTGCTGGCCGA
CGGCTAA

Upstream 100 bases:

>100_bases
CAAGCGCTTATGCGGCTGCGAATGCCGTCTTTGCCTTTTGACGGGTAGCGGCTCGCGGGCCTATGGGGACGCCGGTTCTA
CGAGAAGGAGTATCGCTTCG

Downstream 100 bases:

>100_bases
CGGCGCAGGGCCGTCGAGCGCGCCATCTCGCTCGACCAGCGGCAGAATCGCGTCGACTACGGTGCTGGCCTCGACAGGCC
GTTTGGGCCTAGAGGCAGGC

Product: Fe-S type hydro-lyase tartrate/fumarate alpha region:Fe-S typehydro-lyase tartrate/fumarate beta region

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSDMTTIREDDLIESVADALQYISYYHPMDYIRALGDAYEAEQGPAAKDAIAQILTNSRMCAEGHRPICQDTGIVNVFVK
WGQDCRLVSRRSLQDVVDEGVRRAYNHPDNKLRASILADPAFTRRNTRDNTPCVLSVEMVPGRMVEIDVAAKGGGSENKS
KFKMMNPSDNIVDWVVEQLPSMGAGWCPPGMLGIGIGGTAEHCMRLAKLSLMEPIDMGQLKQRGAQNDLEQLRIDIFDAV
NAQGIGAQGLGGLSTVLDVKILDAPCHAAGKPVGMIPNCAATRHAHFTLDGSGPAYLEQPDLDQWPKVDWQPDSAAKRVD
LDNLTQEEVNSWEHGDRLLLSGKMLTGRDAAHKRIQDMLDKGEELPVDFKGRAIYYVGPVDPVMGEVVGPAGPTTATRMD
KFTEMMLDLGLLAMIGKAERGHDAVEVISRYKVAYLMATGGAAYLVARAIKESRVVGFEDLGMEAIYEFTVKDMPVTVAV
DSQGNNVHTLAPAKWKQRIADEKLLADG

Sequences:

>Translated_508_residues
MSDMTTIREDDLIESVADALQYISYYHPMDYIRALGDAYEAEQGPAAKDAIAQILTNSRMCAEGHRPICQDTGIVNVFVK
WGQDCRLVSRRSLQDVVDEGVRRAYNHPDNKLRASILADPAFTRRNTRDNTPCVLSVEMVPGRMVEIDVAAKGGGSENKS
KFKMMNPSDNIVDWVVEQLPSMGAGWCPPGMLGIGIGGTAEHCMRLAKLSLMEPIDMGQLKQRGAQNDLEQLRIDIFDAV
NAQGIGAQGLGGLSTVLDVKILDAPCHAAGKPVGMIPNCAATRHAHFTLDGSGPAYLEQPDLDQWPKVDWQPDSAAKRVD
LDNLTQEEVNSWEHGDRLLLSGKMLTGRDAAHKRIQDMLDKGEELPVDFKGRAIYYVGPVDPVMGEVVGPAGPTTATRMD
KFTEMMLDLGLLAMIGKAERGHDAVEVISRYKVAYLMATGGAAYLVARAIKESRVVGFEDLGMEAIYEFTVKDMPVTVAV
DSQGNNVHTLAPAKWKQRIADEKLLADG
>Mature_507_residues
SDMTTIREDDLIESVADALQYISYYHPMDYIRALGDAYEAEQGPAAKDAIAQILTNSRMCAEGHRPICQDTGIVNVFVKW
GQDCRLVSRRSLQDVVDEGVRRAYNHPDNKLRASILADPAFTRRNTRDNTPCVLSVEMVPGRMVEIDVAAKGGGSENKSK
FKMMNPSDNIVDWVVEQLPSMGAGWCPPGMLGIGIGGTAEHCMRLAKLSLMEPIDMGQLKQRGAQNDLEQLRIDIFDAVN
AQGIGAQGLGGLSTVLDVKILDAPCHAAGKPVGMIPNCAATRHAHFTLDGSGPAYLEQPDLDQWPKVDWQPDSAAKRVDL
DNLTQEEVNSWEHGDRLLLSGKMLTGRDAAHKRIQDMLDKGEELPVDFKGRAIYYVGPVDPVMGEVVGPAGPTTATRMDK
FTEMMLDLGLLAMIGKAERGHDAVEVISRYKVAYLMATGGAAYLVARAIKESRVVGFEDLGMEAIYEFTVKDMPVTVAVD
SQGNNVHTLAPAKWKQRIADEKLLADG

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=452, Percent_Identity=31.1946902654867, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787897, Length=461, Percent_Identity=29.9349240780911, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789442, Length=291, Percent_Identity=28.1786941580756, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789443, Length=177, Percent_Identity=29.9435028248588, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55509; Mature: 55378

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMTTIREDDLIESVADALQYISYYHPMDYIRALGDAYEAEQGPAAKDAIAQILTNSRM
CCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
CAEGHRPICQDTGIVNVFVKWGQDCRLVSRRSLQDVVDEGVRRAYNHPDNKLRASILADP
HHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
AFTRRNTRDNTPCVLSVEMVPGRMVEIDVAAKGGGSENKSKFKMMNPSDNIVDWVVEQLP
HHHCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCC
SMGAGWCPPGMLGIGIGGTAEHCMRLAKLSLMEPIDMGQLKQRGAQNDLEQLRIDIFDAV
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHC
NAQGIGAQGLGGLSTVLDVKILDAPCHAAGKPVGMIPNCAATRHAHFTLDGSGPAYLEQP
CCCCCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
DLDQWPKVDWQPDSAAKRVDLDNLTQEEVNSWEHGDRLLLSGKMLTGRDAAHKRIQDMLD
CCCCCCCCCCCCCCHHHHCCHHCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHHHHH
KGEELPVDFKGRAIYYVGPVDPVMGEVVGPAGPTTATRMDKFTEMMLDLGLLAMIGKAER
CCCCCCEEECCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GHDAVEVISRYKVAYLMATGGAAYLVARAIKESRVVGFEDLGMEAIYEFTVKDMPVTVAV
CHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCHHHHCHHHHHHHHCCCCCEEEEE
DSQGNNVHTLAPAKWKQRIADEKLLADG
ECCCCEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDMTTIREDDLIESVADALQYISYYHPMDYIRALGDAYEAEQGPAAKDAIAQILTNSRM
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
CAEGHRPICQDTGIVNVFVKWGQDCRLVSRRSLQDVVDEGVRRAYNHPDNKLRASILADP
HHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
AFTRRNTRDNTPCVLSVEMVPGRMVEIDVAAKGGGSENKSKFKMMNPSDNIVDWVVEQLP
HHHCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCC
SMGAGWCPPGMLGIGIGGTAEHCMRLAKLSLMEPIDMGQLKQRGAQNDLEQLRIDIFDAV
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHC
NAQGIGAQGLGGLSTVLDVKILDAPCHAAGKPVGMIPNCAATRHAHFTLDGSGPAYLEQP
CCCCCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
DLDQWPKVDWQPDSAAKRVDLDNLTQEEVNSWEHGDRLLLSGKMLTGRDAAHKRIQDMLD
CCCCCCCCCCCCCCHHHHCCHHCCCHHHHHCCCCCCEEEEECCEECCCHHHHHHHHHHHH
KGEELPVDFKGRAIYYVGPVDPVMGEVVGPAGPTTATRMDKFTEMMLDLGLLAMIGKAER
CCCCCCEEECCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GHDAVEVISRYKVAYLMATGGAAYLVARAIKESRVVGFEDLGMEAIYEFTVKDMPVTVAV
CHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCHHHHCHHHHHHHHCCCCCEEEEE
DSQGNNVHTLAPAKWKQRIADEKLLADG
ECCCCEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]