Click here to switch to the map view.

The map label for this gene is pyrD

Identifier: 85375390

GI number: 85375390

Start: 2587236

End: 2588267

Strand: Direct

Name: pyrD

Synonym: ELI_12815

Alternate gene names: 85375390

Gene position: 2587236-2588267 (Clockwise)

Preceding gene: 85375374

Following gene: 85375391

Centisome position: 84.76

GC content: 66.38

Gene sequence:

>1032_bases
ATGCTTTTTTCGCTCATCCGCCCTGCCATACACGCGCTCGATCCGGAAAGAGCGCACCGGTTTTCGATCGAAGCGCTCAA
GCTGGCGCCGTTGCCGCACAGCCGCCACTCCGACGCTTCGCTGTCCGTCAAAGTCGCCGGCATCCGCTTCCCCAATCCGG
TCGGCGTCGCGGCCGGTTACGACAAGGATGCCGAAGTGCCCGATGCCCTGCTGGGCCTCGGCTTCGGCTTCGTCGAAGTC
GGCTCGATCACACCCCGCCCGCAGGAGGGCAATCCGAAACCGCGCCTGTTCCGGCTGAGCCGCGACCGGGCGGTTATCAA
TCGCATGGGGTTCAACAATGCGGGGGCCGATGTGGCGGAGCGACGGCTGAGGGCCCGCGCGGCGAAGGGCGGCGTGATCG
GCATCAATGTCGGGGCGAACAAGGATTCGGATGATCGCATCGCCGATTACGCGACCATGGTCCGCCGCATGGCGCCTTAT
GCAAGCTATCTCACCGCCAATATCTCCAGCCCCAACACGCCGGGCCTGCGCGCGCTGCAGGACGAAGGGGCGCTGACCGG
ACTGCTCGACGCGGTGATGGAAGCGCCCGGTGCCGACGGTCCGCCGGTCTTCCTCAAGGTCGCGCCCGACCTCGAGCCTG
CCGATGTCGATGCGATTGCGCGGATCGCGATCGACAAGGGCCTCGGTGCGCTGATCGTCTCGAACACCACCATCTTCCGG
CCCGATCTCCAATCGCGCGATCGCGACGAGACGGGAGGCCTCTCCGGCGCGCCCCTCAAACCGCTCGCGCTCCAGCGTTT
GCGCGACTTCCGCAGCGCCACTGGCGGAGCTATTCCGCTGGTCGGCGTAGGCGGGATAGCCACTATAGACGACGCGTGGG
AACGCATTCGCGCAGGGGCGAGCCTCGTGCAGGTGTATTCGGCGATGGTCTACGAGGGCCCCGGCCTCGGGCGCTCCATC
GCGCGCGGGCTTTCGCGCAAATTGCGCGAACACGGCATGGCTTCGATTGAAGAAGCGGTCGGAAGCGAATAG

Upstream 100 bases:

>100_bases
GGAAACGACAAAACCGAAATTGTCCGGTTTTCCGGGCAATATATCCGCAGCCGGGCCTTGCTCGACAGATGCGGAATTGC
GGGCCATAGGGCGCGCACGC

Downstream 100 bases:

>100_bases
CACGCCTTGCCATGACAATTCGCCAAATCCTCGCCTCTCTCGCCGCGCTTGCCCTTGTCGGATGCGCCAATGCCTATGCC
GAAGAGCCGGTTGTTGCGCA

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLFSLIRPAIHALDPERAHRFSIEALKLAPLPHSRHSDASLSVKVAGIRFPNPVGVAAGYDKDAEVPDALLGLGFGFVEV
GSITPRPQEGNPKPRLFRLSRDRAVINRMGFNNAGADVAERRLRARAAKGGVIGINVGANKDSDDRIADYATMVRRMAPY
ASYLTANISSPNTPGLRALQDEGALTGLLDAVMEAPGADGPPVFLKVAPDLEPADVDAIARIAIDKGLGALIVSNTTIFR
PDLQSRDRDETGGLSGAPLKPLALQRLRDFRSATGGAIPLVGVGGIATIDDAWERIRAGASLVQVYSAMVYEGPGLGRSI
ARGLSRKLREHGMASIEEAVGSE

Sequences:

>Translated_343_residues
MLFSLIRPAIHALDPERAHRFSIEALKLAPLPHSRHSDASLSVKVAGIRFPNPVGVAAGYDKDAEVPDALLGLGFGFVEV
GSITPRPQEGNPKPRLFRLSRDRAVINRMGFNNAGADVAERRLRARAAKGGVIGINVGANKDSDDRIADYATMVRRMAPY
ASYLTANISSPNTPGLRALQDEGALTGLLDAVMEAPGADGPPVFLKVAPDLEPADVDAIARIAIDKGLGALIVSNTTIFR
PDLQSRDRDETGGLSGAPLKPLALQRLRDFRSATGGAIPLVGVGGIATIDDAWERIRAGASLVQVYSAMVYEGPGLGRSI
ARGLSRKLREHGMASIEEAVGSE
>Mature_343_residues
MLFSLIRPAIHALDPERAHRFSIEALKLAPLPHSRHSDASLSVKVAGIRFPNPVGVAAGYDKDAEVPDALLGLGFGFVEV
GSITPRPQEGNPKPRLFRLSRDRAVINRMGFNNAGADVAERRLRARAAKGGVIGINVGANKDSDDRIADYATMVRRMAPY
ASYLTANISSPNTPGLRALQDEGALTGLLDAVMEAPGADGPPVFLKVAPDLEPADVDAIARIAIDKGLGALIVSNTTIFR
PDLQSRDRDETGGLSGAPLKPLALQRLRDFRSATGGAIPLVGVGGIATIDDAWERIRAGASLVQVYSAMVYEGPGLGRSI
ARGLSRKLREHGMASIEEAVGSE

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=346, Percent_Identity=47.9768786127168, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1787177, Length=334, Percent_Identity=41.6167664670659, Blast_Score=247, Evalue=8e-67,
Organism=Escherichia coli, GI87082059, Length=327, Percent_Identity=25.0764525993884, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17509475, Length=356, Percent_Identity=42.9775280898876, Blast_Score=267, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6322633, Length=325, Percent_Identity=25.2307692307692, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281361352, Length=351, Percent_Identity=42.7350427350427, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI17137316, Length=351, Percent_Identity=42.7350427350427, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_ERYLH (Q2N6N6)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459452.1
- ProteinModelPortal:   Q2N6N6
- SMR:   Q2N6N6
- STRING:   Q2N6N6
- GeneID:   3869159
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_12815
- NMPDR:   fig|314225.3.peg.462
- eggNOG:   COG0167
- HOGENOM:   HBG351027
- OMA:   AALNRMG
- PhylomeDB:   Q2N6N6
- ProtClustDB:   PRK05286
- BioCyc:   ELIT314225:ELI_12815-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 36305; Mature: 36305

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSLIRPAIHALDPERAHRFSIEALKLAPLPHSRHSDASLSVKVAGIRFPNPVGVAAGY
CHHHHHHHHHHHCCHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCCCC
DKDAEVPDALLGLGFGFVEVGSITPRPQEGNPKPRLFRLSRDRAVINRMGFNNAGADVAE
CCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCHHHHH
RRLRARAAKGGVIGINVGANKDSDDRIADYATMVRRMAPYASYLTANISSPNTPGLRALQ
HHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHH
DEGALTGLLDAVMEAPGADGPPVFLKVAPDLEPADVDAIARIAIDKGLGALIVSNTTIFR
HCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCEEEC
PDLQSRDRDETGGLSGAPLKPLALQRLRDFRSATGGAIPLVGVGGIATIDDAWERIRAGA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHH
SLVQVYSAMVYEGPGLGRSIARGLSRKLREHGMASIEEAVGSE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MLFSLIRPAIHALDPERAHRFSIEALKLAPLPHSRHSDASLSVKVAGIRFPNPVGVAAGY
CHHHHHHHHHHHCCHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCCCC
DKDAEVPDALLGLGFGFVEVGSITPRPQEGNPKPRLFRLSRDRAVINRMGFNNAGADVAE
CCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCHHHHH
RRLRARAAKGGVIGINVGANKDSDDRIADYATMVRRMAPYASYLTANISSPNTPGLRALQ
HHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHH
DEGALTGLLDAVMEAPGADGPPVFLKVAPDLEPADVDAIARIAIDKGLGALIVSNTTIFR
HCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCEEEC
PDLQSRDRDETGGLSGAPLKPLALQRLRDFRSATGGAIPLVGVGGIATIDDAWERIRAGA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHH
SLVQVYSAMVYEGPGLGRSIARGLSRKLREHGMASIEEAVGSE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA