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The map label for this gene is thcD [H]

Identifier: 85375311

GI number: 85375311

Start: 2512078

End: 2513280

Strand: Direct

Name: thcD [H]

Synonym: ELI_12420

Alternate gene names: 85375311

Gene position: 2512078-2513280 (Clockwise)

Preceding gene: 85375310

Following gene: 85375312

Centisome position: 82.3

GC content: 65.34

Gene sequence:

>1203_bases
GTGGGGGCAGGGCACGGCGGCGCGCAGGCGGCCATCGCCCTGCGGCAAAAGGGTTTCGAAGGCAGCATCGCGCTTCTGAC
GCGGGAGAAGGTCCCGCCGTACGAGCGCCCGCCGCTGTCCAAGGAGTACTTTGCCGACGAAAAACCGTTCGAGCGTATCC
TGATCCGTCCCGAGACATTCTGGGCCGACAAGCAGATCGACCTGCAACTCGGGCGCGCGGTGAGCGAGGTCGATGCGACG
GCGCATGCGCTCACCTTCACCGATGGCAGCACCATGGAATACGGCCAACTGATCTGGGCCGCCGGCGGCGACGCGCGACG
CCTGTCGTGTTCGGGTGCCGAGCTCGAGGGCGTGCACGCCATCCGTAACCGCCCCGATGTCGACCTACTGAAAAGCGAGC
TGGATGCCGGTAGGCGCAAGGCCGTGGTGATCGGCGGTGGTTACATCGGCCTCGAAGCAGCGGCGGTCCTGCGCAAGCTC
GGGTGCGAGGTGACGGTACTCGAGGCGCTGCCGAGAGTGCTGTCGCGGGTTGCCGGAGAAGATCTTTCCGCCTTCTTCGA
ATCCGAGCACCGCGCTCATGGTGTCGATGTTCGCACGGAGGCTGCCGTCGAAGCGCTCGAAGGCGATGGCAGCCGCATAA
CGGGCGTAAAGCTCGAAGGCGATGAAATGGTGCCAGCCGATTTCGTTATTGTCGGGATCGGCATCGTCCCGTCGATCGGC
CCGCTGATCGCGGCGGGAGCGGCGGGGTCCAACGGGGTCGATGTGGACGAATTCTGCCGCACCAGCCTGGAGGATATTTA
CGCCATCGGCGACTGTGCGGCGCATGCCAATCGCTATGCCGACGGGGCGGTGATCCGGCTGGAATCGGTCCAGAATGCCA
ACGACATGGCGACGACCGTGGCCCGCCACGTCACCGGCGATCCGGAGCCTTACGGCGCGGTGCCGTGGTTCTGGTCCAAC
CAGTACGACCTGAGGTTGCAGACGGTCGGCTTTTCCATGCAGCATGACGAGACCGTGCTGCGCGGCAATCCGGCGGAGCG
CAGCTTCAGCGTCGTCTATCTGCGCGAAGGGCGCGTGATCGCGCTCGACTGCGTCAACCGGACGAAGGACTATGCGCAGG
GGCGCAAACTGGTCGAAGCGCGGCTGGAGGCGAGCCCCGAACAGCTCGCCGATATCGAGACACCGCTCAAGGAACTGCTG
TGA

Upstream 100 bases:

>100_bases
CAGGCGCGCAACGTCCTGTCGCGCTGAACCCGAACAGGCTTGACGAGCGGGGCGCACCACCCCCATTCCGCTGCGCATGG
ATCGAGCGGACATTTGCATC

Downstream 100 bases:

>100_bases
GGAGGGCGCCCTTATGATCGCACCCGGTGACGGCAAGCCCATGCTCGAAGGCGTGACGGTCATCGACCTCACCAGCATGG
TGTTCGGCCCCTATTGCACG

Product: putative ferredoxin reductase component (dioxygenase)

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MGAGHGGAQAAIALRQKGFEGSIALLTREKVPPYERPPLSKEYFADEKPFERILIRPETFWADKQIDLQLGRAVSEVDAT
AHALTFTDGSTMEYGQLIWAAGGDARRLSCSGAELEGVHAIRNRPDVDLLKSELDAGRRKAVVIGGGYIGLEAAAVLRKL
GCEVTVLEALPRVLSRVAGEDLSAFFESEHRAHGVDVRTEAAVEALEGDGSRITGVKLEGDEMVPADFVIVGIGIVPSIG
PLIAAGAAGSNGVDVDEFCRTSLEDIYAIGDCAAHANRYADGAVIRLESVQNANDMATTVARHVTGDPEPYGAVPWFWSN
QYDLRLQTVGFSMQHDETVLRGNPAERSFSVVYLREGRVIALDCVNRTKDYAQGRKLVEARLEASPEQLADIETPLKELL

Sequences:

>Translated_400_residues
MGAGHGGAQAAIALRQKGFEGSIALLTREKVPPYERPPLSKEYFADEKPFERILIRPETFWADKQIDLQLGRAVSEVDAT
AHALTFTDGSTMEYGQLIWAAGGDARRLSCSGAELEGVHAIRNRPDVDLLKSELDAGRRKAVVIGGGYIGLEAAAVLRKL
GCEVTVLEALPRVLSRVAGEDLSAFFESEHRAHGVDVRTEAAVEALEGDGSRITGVKLEGDEMVPADFVIVGIGIVPSIG
PLIAAGAAGSNGVDVDEFCRTSLEDIYAIGDCAAHANRYADGAVIRLESVQNANDMATTVARHVTGDPEPYGAVPWFWSN
QYDLRLQTVGFSMQHDETVLRGNPAERSFSVVYLREGRVIALDCVNRTKDYAQGRKLVEARLEASPEQLADIETPLKELL
>Mature_399_residues
GAGHGGAQAAIALRQKGFEGSIALLTREKVPPYERPPLSKEYFADEKPFERILIRPETFWADKQIDLQLGRAVSEVDATA
HALTFTDGSTMEYGQLIWAAGGDARRLSCSGAELEGVHAIRNRPDVDLLKSELDAGRRKAVVIGGGYIGLEAAAVLRKLG
CEVTVLEALPRVLSRVAGEDLSAFFESEHRAHGVDVRTEAAVEALEGDGSRITGVKLEGDEMVPADFVIVGIGIVPSIGP
LIAAGAAGSNGVDVDEFCRTSLEDIYAIGDCAAHANRYADGAVIRLESVQNANDMATTVARHVTGDPEPYGAVPWFWSNQ
YDLRLQTVGFSMQHDETVLRGNPAERSFSVVYLREGRVIALDCVNRTKDYAQGRKLVEARLEASPEQLADIETPLKELL

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=372, Percent_Identity=30.3763440860215, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI21389617, Length=372, Percent_Identity=30.3763440860215, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI65787454, Length=372, Percent_Identity=30.3763440860215, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI22202629, Length=327, Percent_Identity=25.3822629969419, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI4757732, Length=327, Percent_Identity=25.3822629969419, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=33.5, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789065, Length=312, Percent_Identity=24.6794871794872, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789765, Length=264, Percent_Identity=30.3030303030303, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=366, Percent_Identity=26.775956284153, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24585130, Length=402, Percent_Identity=25.3731343283582, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24639257, Length=367, Percent_Identity=26.7029972752044, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281359715, Length=367, Percent_Identity=26.9754768392371, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281359713, Length=367, Percent_Identity=26.9754768392371, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24639250, Length=367, Percent_Identity=26.9754768392371, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI18543267, Length=367, Percent_Identity=26.9754768392371, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24639252, Length=367, Percent_Identity=26.9754768392371, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 43198; Mature: 43067

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAGHGGAQAAIALRQKGFEGSIALLTREKVPPYERPPLSKEYFADEKPFERILIRPETF
CCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCHHEEECCCCC
WADKQIDLQLGRAVSEVDATAHALTFTDGSTMEYGQLIWAAGGDARRLSCSGAELEGVHA
CCCCEEHHHHHHHHHHHHCHHEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCCCCHHH
IRNRPDVDLLKSELDAGRRKAVVIGGGYIGLEAAAVLRKLGCEVTVLEALPRVLSRVAGE
HCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCH
DLSAFFESEHRAHGVDVRTEAAVEALEGDGSRITGVKLEGDEMVPADFVIVGIGIVPSIG
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECCHHCCHH
PLIAAGAAGSNGVDVDEFCRTSLEDIYAIGDCAAHANRYADGAVIRLESVQNANDMATTV
HHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
ARHVTGDPEPYGAVPWFWSNQYDLRLQTVGFSMQHDETVLRGNPAERSFSVVYLREGRVI
HHHCCCCCCCCCCCCCEECCCEEEEEEEECCCCCCCCEEECCCCCCCCEEEEEEECCCEE
ALDCVNRTKDYAQGRKLVEARLEASPEQLADIETPLKELL
EEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
GAGHGGAQAAIALRQKGFEGSIALLTREKVPPYERPPLSKEYFADEKPFERILIRPETF
CCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCHHEEECCCCC
WADKQIDLQLGRAVSEVDATAHALTFTDGSTMEYGQLIWAAGGDARRLSCSGAELEGVHA
CCCCEEHHHHHHHHHHHHCHHEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCCCCHHH
IRNRPDVDLLKSELDAGRRKAVVIGGGYIGLEAAAVLRKLGCEVTVLEALPRVLSRVAGE
HCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCH
DLSAFFESEHRAHGVDVRTEAAVEALEGDGSRITGVKLEGDEMVPADFVIVGIGIVPSIG
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECCHHCCHH
PLIAAGAAGSNGVDVDEFCRTSLEDIYAIGDCAAHANRYADGAVIRLESVQNANDMATTV
HHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
ARHVTGDPEPYGAVPWFWSNQYDLRLQTVGFSMQHDETVLRGNPAERSFSVVYLREGRVI
HHHCCCCCCCCCCCCCEECCCEEEEEEEECCCCCCCCEEECCCCCCCCEEEEEEECCCEE
ALDCVNRTKDYAQGRKLVEARLEASPEQLADIETPLKELL
EEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]