Click here to switch to the map view.

The map label for this gene is 85375082

Identifier: 85375082

GI number: 85375082

Start: 2280208

End: 2281059

Strand: Reverse

Name: 85375082

Synonym: ELI_11275

Alternate gene names: NA

Gene position: 2281059-2280208 (Counterclockwise)

Preceding gene: 85375083

Following gene: 85375081

Centisome position: 74.73

GC content: 62.21

Gene sequence:

>852_bases
ATGAGAGAGATCACCGTAGCCGCCTTGCAGCTGCCGCTCGGCAGCGAGGATGAGCAGGCCAATATCGATGCCGTGGCCGA
GCTGGTCGAGCAGGCCGCCGCACGCGGGGCCGAAATCGTGCTGCCGCCGGAGCTCTTTTCCGGCCCCTATTTCTGCCAAG
TCGAAGACGAGGCGCTGTTCGCGCTGGCGCGCCCGACGCTGGAACATCCCAGCGTCGTCGCGATGCGCAAGCTGGCGGGC
AAGCTCGAGATCGCCATCCCCACCAGCTTCTTCGAGCGCGACGGGCATCACTATTACAACACGCTCGCCATGATCGGGCC
CGATGGCGAACTTCAGGGGACCTACCGCAAAAGCCACATTCCCGATGGTCCCGGGTATGAAGAGAAGTTCTATTTCCGCC
CGGGCAATGACGGGTTCAAGGTGTGGGATGTGTGCGGCACGCGCATCGGCGTCGGGGTGTGCTGGGACCAGTGGTATCCC
GAATGCGCGCGGGTAATGGCGCTGATGGGCGCCGAATTGCTGTTCTACCCGACCGCCATCGGTTCCGAGCCCTACGACGC
CGATTTCGATACCAGCCGCATGTGGCAGCGCTCGATGCAGGGCCACAGCGTGTCCAATTGCATGCCGGTGATCGCCGCCA
ATCGTATCGGCACCGAAGGTGAGGCAAAATTCTACGGCCACAGTTTCATCACCAATGAATGGGGGGACAAGATCGTCGAG
TTCGGTGCCGAAGAAGACGGTGTGCTGGTGGCAACCCTCGATCTCGACGAAGCTGCGAAACACCGTGCCGGGATGGGCTT
TTTCCGCGATCGCCGCCCGCAGCTCTATAGGCGGATTTGCGAGGACATCTGA

Upstream 100 bases:

>100_bases
GCCGCCGCCGGAAGAAGGCGCTCCCTTCATGGCCGGCACCACAACCAGCAGCGTATCGGTGCAAGTGGACTTCGCCCTGC
GCCCGCTCTAACGGCGGCGA

Downstream 100 bases:

>100_bases
GCAGCACCTATCTCGTCGCGCTCGGAAGCAACCGGCCGCATCACCTCTATGGCCGTCCGCGCGCGGTCGTGCATGCAGCG
ATGGAAGAGCTGGCGGTTCT

Product: hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MREITVAALQLPLGSEDEQANIDAVAELVEQAAARGAEIVLPPELFSGPYFCQVEDEALFALARPTLEHPSVVAMRKLAG
KLEIAIPTSFFERDGHHYYNTLAMIGPDGELQGTYRKSHIPDGPGYEEKFYFRPGNDGFKVWDVCGTRIGVGVCWDQWYP
ECARVMALMGAELLFYPTAIGSEPYDADFDTSRMWQRSMQGHSVSNCMPVIAANRIGTEGEAKFYGHSFITNEWGDKIVE
FGAEEDGVLVATLDLDEAAKHRAGMGFFRDRRPQLYRRICEDI

Sequences:

>Translated_283_residues
MREITVAALQLPLGSEDEQANIDAVAELVEQAAARGAEIVLPPELFSGPYFCQVEDEALFALARPTLEHPSVVAMRKLAG
KLEIAIPTSFFERDGHHYYNTLAMIGPDGELQGTYRKSHIPDGPGYEEKFYFRPGNDGFKVWDVCGTRIGVGVCWDQWYP
ECARVMALMGAELLFYPTAIGSEPYDADFDTSRMWQRSMQGHSVSNCMPVIAANRIGTEGEAKFYGHSFITNEWGDKIVE
FGAEEDGVLVATLDLDEAAKHRAGMGFFRDRRPQLYRRICEDI
>Mature_283_residues
MREITVAALQLPLGSEDEQANIDAVAELVEQAAARGAEIVLPPELFSGPYFCQVEDEALFALARPTLEHPSVVAMRKLAG
KLEIAIPTSFFERDGHHYYNTLAMIGPDGELQGTYRKSHIPDGPGYEEKFYFRPGNDGFKVWDVCGTRIGVGVCWDQWYP
ECARVMALMGAELLFYPTAIGSEPYDADFDTSRMWQRSMQGHSVSNCMPVIAANRIGTEGEAKFYGHSFITNEWGDKIVE
FGAEEDGVLVATLDLDEAAKHRAGMGFFRDRRPQLYRRICEDI

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=283, Percent_Identity=30.3886925795053, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI297632348, Length=293, Percent_Identity=28.3276450511945, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI297632350, Length=293, Percent_Identity=28.3276450511945, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI5031947, Length=293, Percent_Identity=28.3276450511945, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI7706509, Length=275, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI297632346, Length=198, Percent_Identity=28.7878787878788, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17533173, Length=311, Percent_Identity=27.9742765273312, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17556280, Length=281, Percent_Identity=25.6227758007117, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=291, Percent_Identity=26.4604810996564, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21358471, Length=277, Percent_Identity=27.7978339350181, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355835, Length=281, Percent_Identity=27.7580071174377, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17933642, Length=280, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31629; Mature: 31629

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50263 CN_HYDROLASE ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREITVAALQLPLGSEDEQANIDAVAELVEQAAARGAEIVLPPELFSGPYFCQVEDEALF
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECHHHHCCCEEEEECCHHHH
ALARPTLEHPSVVAMRKLAGKLEIAIPTSFFERDGHHYYNTLAMIGPDGELQGTYRKSHI
HHHCCCCCCCHHHHHHHHCCEEEEEECHHHHHCCCCEEEEEEEEECCCCCCCCCCHHCCC
PDGPGYEEKFYFRPGNDGFKVWDVCGTRIGVGVCWDQWYPECARVMALMGAELLFYPTAI
CCCCCCCCEEEECCCCCCCEEHHHHCCHHCCEEEHHHHHHHHHHHHHHHCCHHEEEEHHC
GSEPYDADFDTSRMWQRSMQGHSVSNCMPVIAANRIGTEGEAKFYGHSFITNEWGDKIVE
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCHHHHCHHHHHHHH
FGAEEDGVLVATLDLDEAAKHRAGMGFFRDRRPQLYRRICEDI
CCCCCCCEEEEEECHHHHHHHHCCCCHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MREITVAALQLPLGSEDEQANIDAVAELVEQAAARGAEIVLPPELFSGPYFCQVEDEALF
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECHHHHCCCEEEEECCHHHH
ALARPTLEHPSVVAMRKLAGKLEIAIPTSFFERDGHHYYNTLAMIGPDGELQGTYRKSHI
HHHCCCCCCCHHHHHHHHCCEEEEEECHHHHHCCCCEEEEEEEEECCCCCCCCCCHHCCC
PDGPGYEEKFYFRPGNDGFKVWDVCGTRIGVGVCWDQWYPECARVMALMGAELLFYPTAI
CCCCCCCCEEEECCCCCCCEEHHHHCCHHCCEEEHHHHHHHHHHHHHHHCCHHEEEEHHC
GSEPYDADFDTSRMWQRSMQGHSVSNCMPVIAANRIGTEGEAKFYGHSFITNEWGDKIVE
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCHHHHCHHHHHHHH
FGAEEDGVLVATLDLDEAAKHRAGMGFFRDRRPQLYRRICEDI
CCCCCCCEEEEEECHHHHHHHHCCCCHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]