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The map label for this gene is pcbC [H]

Identifier: 85375063

GI number: 85375063

Start: 2258461

End: 2259390

Strand: Reverse

Name: pcbC [H]

Synonym: ELI_11180

Alternate gene names: 85375063

Gene position: 2259390-2258461 (Counterclockwise)

Preceding gene: 85375064

Following gene: 85375062

Centisome position: 74.02

GC content: 62.47

Gene sequence:

>930_bases
ATGACCGACATTGCTTCCGTTTCACTCGACCAGCCGCTCGAAAGCATCGCCGGCGAGCTTGGCGCGTCTTTCTCCGAATT
CGGCTTTGCCATCGTCAAGGATCACGGCATCCCGCAGGATCTGATCGACCGGGCGGAAGAGATGTCGAAGGCCTTCTTTG
CGCTGCCAACCGAGACCAAGCTCAAGTACAAGATCGAAGGCGCCGGCGGGGCACGCGGCTATACGCCGTTCGGAACCGAA
AAGGCCAAGGATGCGAAGGTCCATGACCTGAAGGAGTTCTGGCACGTCGGCCGCTCGCTGCCCGCCGATCACGAACTGGC
CGAATATATGGCACCCAATGTCTGGCCTACCGAAGTCGACGGCTTCGAAGAGACCTTCGAAGCGCTCTACGCGGCGTTCG
AACAAGCCGGCCTGCGCGTGCTGGAGGCGATTGCCATCCATCTCGGGCTGGAGCGCACCTTCTTCGAAAAGACGGTCGAG
GACGGCAATTCGGTCATGCGCCTGCTGCGCTATCCGCCGCTTGAAGGCGAAGAAGCCGAGGGCGCGATCCGCGCCGCCGC
CCATGGCGACATCAATACCATCACGCTGTTGCTGGGGGCCGAGGAGGCCGGGCTCGAACTGCTGACGAAGCAGGGCGAGT
GGTTGGCGGTAAACCCGCCCCCGGGCGCGCTGGCGGTAAATATCGGCGATATGCTCGACCGGCTTACCAACGGCAAGCTG
CGCTCGACGACGCACAGGGTCGTCAACCCGCACGGCGAAGCGGCCTATCGCGCGCGCTATTCGATGCCGTTCTTCCTGCA
TTTCCGGCCCGATTTCATCATCGAGCCGCTGGAATCCTGTTCGGAATCCGTGACCGGGAATACGCCCCAGCCGGCGATCT
CATCGCACGACTTTCTCCTGCAGCGCCTGCGCGAGATCAATCTGGCCTGA

Upstream 100 bases:

>100_bases
ATCGCCAAGCTCGGTCTCAGAGCGCTCGCCGCCGGCTCGCTGGCCAACCTGATGAGTGCCGCCTTGGCGGGCCTGTTCCT
CCCTTACTAAGATAAGTGCC

Downstream 100 bases:

>100_bases
TCCTGCGCCCTTGATCGGCCTGTGCATAAGAATAAGAGCGCTTGTTGCAATGCAGCAACACCACATAACCCTCTGAAAAA
CTTTACACTTTTCATGACAC

Product: oxidoreductase iron/ascorbate family protein

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTDIASVSLDQPLESIAGELGASFSEFGFAIVKDHGIPQDLIDRAEEMSKAFFALPTETKLKYKIEGAGGARGYTPFGTE
KAKDAKVHDLKEFWHVGRSLPADHELAEYMAPNVWPTEVDGFEETFEALYAAFEQAGLRVLEAIAIHLGLERTFFEKTVE
DGNSVMRLLRYPPLEGEEAEGAIRAAAHGDINTITLLLGAEEAGLELLTKQGEWLAVNPPPGALAVNIGDMLDRLTNGKL
RSTTHRVVNPHGEAAYRARYSMPFFLHFRPDFIIEPLESCSESVTGNTPQPAISSHDFLLQRLREINLA

Sequences:

>Translated_309_residues
MTDIASVSLDQPLESIAGELGASFSEFGFAIVKDHGIPQDLIDRAEEMSKAFFALPTETKLKYKIEGAGGARGYTPFGTE
KAKDAKVHDLKEFWHVGRSLPADHELAEYMAPNVWPTEVDGFEETFEALYAAFEQAGLRVLEAIAIHLGLERTFFEKTVE
DGNSVMRLLRYPPLEGEEAEGAIRAAAHGDINTITLLLGAEEAGLELLTKQGEWLAVNPPPGALAVNIGDMLDRLTNGKL
RSTTHRVVNPHGEAAYRARYSMPFFLHFRPDFIIEPLESCSESVTGNTPQPAISSHDFLLQRLREINLA
>Mature_308_residues
TDIASVSLDQPLESIAGELGASFSEFGFAIVKDHGIPQDLIDRAEEMSKAFFALPTETKLKYKIEGAGGARGYTPFGTEK
AKDAKVHDLKEFWHVGRSLPADHELAEYMAPNVWPTEVDGFEETFEALYAAFEQAGLRVLEAIAIHLGLERTFFEKTVED
GNSVMRLLRYPPLEGEEAEGAIRAAAHGDINTITLLLGAEEAGLELLTKQGEWLAVNPPPGALAVNIGDMLDRLTNGKLR
STTHRVVNPHGEAAYRARYSMPFFLHFRPDFIIEPLESCSESVTGNTPQPAISSHDFLLQRLREINLA

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI85816264, Length=300, Percent_Identity=29.3333333333333, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28572124, Length=282, Percent_Identity=28.0141843971631, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 34036; Mature: 33905

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIASVSLDQPLESIAGELGASFSEFGFAIVKDHGIPQDLIDRAEEMSKAFFALPTETK
CCCCCCCCHHHHHHHHHHHHCCCHHHHCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
LKYKIEGAGGARGYTPFGTEKAKDAKVHDLKEFWHVGRSLPADHELAEYMAPNVWPTEVD
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
GFEETFEALYAAFEQAGLRVLEAIAIHLGLERTFFEKTVEDGNSVMRLLRYPPLEGEEAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GAIRAAAHGDINTITLLLGAEEAGLELLTKQGEWLAVNPPPGALAVNIGDMLDRLTNGKL
CCEEHHCCCCCEEEEEEEECCHHHHHHHHCCCCEEEECCCCCEEEEEHHHHHHHHCCCCH
RSTTHRVVNPHGEAAYRARYSMPFFLHFRPDFIIEPLESCSESVTGNTPQPAISSHDFLL
HHHHHHHCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
QRLREINLA
HHHHHHCCC
>Mature Secondary Structure 
TDIASVSLDQPLESIAGELGASFSEFGFAIVKDHGIPQDLIDRAEEMSKAFFALPTETK
CCCCCCCHHHHHHHHHHHHCCCHHHHCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
LKYKIEGAGGARGYTPFGTEKAKDAKVHDLKEFWHVGRSLPADHELAEYMAPNVWPTEVD
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
GFEETFEALYAAFEQAGLRVLEAIAIHLGLERTFFEKTVEDGNSVMRLLRYPPLEGEEAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GAIRAAAHGDINTITLLLGAEEAGLELLTKQGEWLAVNPPPGALAVNIGDMLDRLTNGKL
CCEEHHCCCCCEEEEEEEECCHHHHHHHHCCCCEEEECCCCCEEEEEHHHHHHHHCCCCH
RSTTHRVVNPHGEAAYRARYSMPFFLHFRPDFIIEPLESCSESVTGNTPQPAISSHDFLL
HHHHHHHCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
QRLREINLA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2308852 [H]