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The map label for this gene is ywnE [H]

Identifier: 85375022

GI number: 85375022

Start: 2218694

End: 2219896

Strand: Reverse

Name: ywnE [H]

Synonym: ELI_10975

Alternate gene names: 85375022

Gene position: 2219896-2218694 (Counterclockwise)

Preceding gene: 85375025

Following gene: 85375020

Centisome position: 72.73

GC content: 61.93

Gene sequence:

>1203_bases
ATGGGGGAGACGAGACAGGTGCATACCGAGCCCGATCCGGTCGAATACAGCTATCCCGAACCGTTCTGCGTGGAAGCGGG
CGACCATACGCTGACCTTCCTGCCCGACGGGGTGCATCGCTTCGACGCATTGCTGGAGCTTGTCCAGGAAGCACAGGAAA
GCCTGCACCTGTTCTACTACATGTTCCAGGATGACGAAGCGGGCAATCCCGTGCGCGATGCGCTGGTGGAAGCCGCCGAG
CGCGGCGTGACCGTGTGCCTGGTGGTGGATCGCTTCGGGACCGATGCGGACAAGGAATTTTTCCAGCCGATCGTCGATGC
AGGCGGCAGCTTCGCCTTCTTCAACCCCAAGAAGAGTCGGCGCTACCTGATCCGCAACCACCAGAAGATGGCCATAGCCG
ATCGCGAAAAAGCGCTGGTTGGCGGGTTCAACGTGTCGGAGCACTACTTCAAGCCGCCCGAGGAAAACGGCTGGTGCGAC
ATGGGCGTGATAGTGCGAGGGCCGCTGGTCGAGCAATTGCTCGATTGGTATGGTCAGATCTACGATTGGGCGACCACGCC
GTCGGCGCAGTACCGCGCGATCCGCACGATGGTGCGCGACTGGGAGCCGGGGGATGGCAAATACCAGCTGCTGGTCGGCG
GCCCGACCTCGATCCCCAGCAACTGGGCGCGCCGGGTGAAGAAGGATCTCGCCCATGCCAGCAAACTCGATCTCGTCATG
GCCTATTTCTCCCCGCCGCGCAGTTTCCGGCGCCTGATCCGCCGGGTCGGGGAGCGGGGCAAGGTGCGGCTGGTGATGGC
CGCCAAGTCGGACAATCCGGCGACCGTCTCGGCAGCACGCGCGACCTATGGCTCAATGCTGCGGGCGGGCTGCGAGGTCT
ATGAATTCCAGCCGTGCAAGCTGCACATGAAGCTGCTGGTGATCGACGATGCGGTCTATTTCGGCAGCGCCAATTTCGAC
CATCGCTCGATCCGCCTCAACCTGGAACTGATGTTCCGTGTCGAGGACCCGGACCTGGCCAAGATGGTGCGCGAATTCGT
CGACGGCATGACCGAGGTCTCGGAGCAGATCACCTACGAATCGCACAAGAAGAATCGCACCTGGCTTTCGACCCTGAAGA
GCTATTTCGGGTGGTTCCTGGTGACCACGGTCGATTACACCGTCAGCAGCACGCTCAATGTCGGCAGCGAAGGTGCGCCG
TAA

Upstream 100 bases:

>100_bases
GGGCACTCCAGACAAAAATGAATTTCGGGATTGCGCGCGAAATAGGGTGGGGCTCGGAACGAGTCAAGAAATCGTGCGCT
AATGGGGTGCGCGCTAAGCG

Downstream 100 bases:

>100_bases
GCTGACCTAACCCTCGCCCCAGCTCGAATAGTCCTTCATCACGGGCGAGGTGAACTTGGTGAATTCGCTCTGGAAATGCA
GCGGTACATTGCCGGTCGAG

Product: phospholipase D family protein

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MGETRQVHTEPDPVEYSYPEPFCVEAGDHTLTFLPDGVHRFDALLELVQEAQESLHLFYYMFQDDEAGNPVRDALVEAAE
RGVTVCLVVDRFGTDADKEFFQPIVDAGGSFAFFNPKKSRRYLIRNHQKMAIADREKALVGGFNVSEHYFKPPEENGWCD
MGVIVRGPLVEQLLDWYGQIYDWATTPSAQYRAIRTMVRDWEPGDGKYQLLVGGPTSIPSNWARRVKKDLAHASKLDLVM
AYFSPPRSFRRLIRRVGERGKVRLVMAAKSDNPATVSAARATYGSMLRAGCEVYEFQPCKLHMKLLVIDDAVYFGSANFD
HRSIRLNLELMFRVEDPDLAKMVREFVDGMTEVSEQITYESHKKNRTWLSTLKSYFGWFLVTTVDYTVSSTLNVGSEGAP

Sequences:

>Translated_400_residues
MGETRQVHTEPDPVEYSYPEPFCVEAGDHTLTFLPDGVHRFDALLELVQEAQESLHLFYYMFQDDEAGNPVRDALVEAAE
RGVTVCLVVDRFGTDADKEFFQPIVDAGGSFAFFNPKKSRRYLIRNHQKMAIADREKALVGGFNVSEHYFKPPEENGWCD
MGVIVRGPLVEQLLDWYGQIYDWATTPSAQYRAIRTMVRDWEPGDGKYQLLVGGPTSIPSNWARRVKKDLAHASKLDLVM
AYFSPPRSFRRLIRRVGERGKVRLVMAAKSDNPATVSAARATYGSMLRAGCEVYEFQPCKLHMKLLVIDDAVYFGSANFD
HRSIRLNLELMFRVEDPDLAKMVREFVDGMTEVSEQITYESHKKNRTWLSTLKSYFGWFLVTTVDYTVSSTLNVGSEGAP
>Mature_399_residues
GETRQVHTEPDPVEYSYPEPFCVEAGDHTLTFLPDGVHRFDALLELVQEAQESLHLFYYMFQDDEAGNPVRDALVEAAER
GVTVCLVVDRFGTDADKEFFQPIVDAGGSFAFFNPKKSRRYLIRNHQKMAIADREKALVGGFNVSEHYFKPPEENGWCDM
GVIVRGPLVEQLLDWYGQIYDWATTPSAQYRAIRTMVRDWEPGDGKYQLLVGGPTSIPSNWARRVKKDLAHASKLDLVMA
YFSPPRSFRRLIRRVGERGKVRLVMAAKSDNPATVSAARATYGSMLRAGCEVYEFQPCKLHMKLLVIDDAVYFGSANFDH
RSIRLNLELMFRVEDPDLAKMVREFVDGMTEVSEQITYESHKKNRTWLSTLKSYFGWFLVTTVDYTVSSTLNVGSEGAP

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=368, Percent_Identity=25, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081826, Length=423, Percent_Identity=26.4775413711584, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787502, Length=332, Percent_Identity=25, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 45503; Mature: 45372

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGETRQVHTEPDPVEYSYPEPFCVEAGDHTLTFLPDGVHRFDALLELVQEAQESLHLFYY
CCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCEEEEEE
MFQDDEAGNPVRDALVEAAERGVTVCLVVDRFGTDADKEFFQPIVDAGGSFAFFNPKKSR
EEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCHH
RYLIRNHQKMAIADREKALVGGFNVSEHYFKPPEENGWCDMGVIVRGPLVEQLLDWYGQI
HHHHHCCCEEEECCCCHHEECCCCCCCCCCCCCCCCCCEEECEEECCHHHHHHHHHHHHH
YDWATTPSAQYRAIRTMVRDWEPGDGKYQLLVGGPTSIPSNWARRVKKDLAHASKLDLVM
HCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEE
AYFSPPRSFRRLIRRVGERGKVRLVMAAKSDNPATVSAARATYGSMLRAGCEVYEFQPCK
EECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHCCCCEEEECCCE
LHMKLLVIDDAVYFGSANFDHRSIRLNLELMFRVEDPDLAKMVREFVDGMTEVSEQITYE
EEEEEEEEECEEEECCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
SHKKNRTWLSTLKSYFGWFLVTTVDYTVSSTLNVGSEGAP
HHCCCHHHHHHHHHHHHEEEEEEEHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GETRQVHTEPDPVEYSYPEPFCVEAGDHTLTFLPDGVHRFDALLELVQEAQESLHLFYY
CCCCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCEEEEEE
MFQDDEAGNPVRDALVEAAERGVTVCLVVDRFGTDADKEFFQPIVDAGGSFAFFNPKKSR
EEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCHH
RYLIRNHQKMAIADREKALVGGFNVSEHYFKPPEENGWCDMGVIVRGPLVEQLLDWYGQI
HHHHHCCCEEEECCCCHHEECCCCCCCCCCCCCCCCCCEEECEEECCHHHHHHHHHHHHH
YDWATTPSAQYRAIRTMVRDWEPGDGKYQLLVGGPTSIPSNWARRVKKDLAHASKLDLVM
HCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEE
AYFSPPRSFRRLIRRVGERGKVRLVMAAKSDNPATVSAARATYGSMLRAGCEVYEFQPCK
EECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHCCCCEEEECCCE
LHMKLLVIDDAVYFGSANFDHRSIRLNLELMFRVEDPDLAKMVREFVDGMTEVSEQITYE
EEEEEEEEECEEEECCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
SHKKNRTWLSTLKSYFGWFLVTTVDYTVSSTLNVGSEGAP
HHCCCHHHHHHHHHHHHEEEEEEEHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]