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The map label for this gene is gcvA [C]

Identifier: 85374530

GI number: 85374530

Start: 1743471

End: 1744355

Strand: Direct

Name: gcvA [C]

Synonym: ELI_08515

Alternate gene names: 85374530

Gene position: 1743471-1744355 (Clockwise)

Preceding gene: 85374529

Following gene: 85374534

Centisome position: 57.12

GC content: 65.42

Gene sequence:

>885_bases
ATGCCCCTGCGACGCCTCCCCCCGCTGCGCGCTCTCGAAGCCTTCATGCGCACTGTCCGCCTCGGCTCGGCCCGCGCCGC
AGCGGAGGAACTCAATCTCAGCCCCAGCGCGCTGTCTCGGCGGATCGCCAATCTCGAGGAGTTCGTCGGCAAGAAGCTGT
TTACCCGCGCCAAGCAGGCCATGGGCCTGACCGACGAAGGACACCAGTTCTACGAGGCCGTGAGCCCGCATTTCGAAGCG
CTGCAACGCGCGGTCGAGGGGCAGTCGGACAATCTCTCGCTGCTGCGCCTGCGCCTGGGCGTCTTGCCGCTGTTCGGCAG
CCAGCGCCTGTTCCCTCGCCTGGGCGAACTGCGCGCGCGGCATAAACTCCTGCATATCGATATCGATACCGGCCCGCATC
TCGAAGACCGGGTCGGCGACACGCTGGATGCGGCGATCATCCTCTCGCGCGGGCCCGAGCGCGGCCTGCACGCCGTGCGG
CTCGACGAGAACAAGGTGCACGCAATCTGCAGCAAGGAGCTTGCTGCGGATATCGGGACCAATCCCGATCAGGATACGCT
CGCGAAGCAAACCTTCCTGATCCACAACGAGCTGCCCGAGAGCTTCATGGCGTGGAAGGACGAGCTCGACATGCGCGATC
TCGATCCGGCGGCGATCGATCATTACGACAGCGGCCAGCTGATGCTGGAGGCCGCCGCACAGGGCCTCGGCATCGCCATC
ATGCATGACGACCACCTGCGCCGCGCCGATGACGACCGGCTGACCGAGCTGTTCGATCTCGATGTCGACAGCCCCTATAG
CTACTGGTTCGTCTGCAAGCCGACCGCACTGGAAAGCCGCCCGGTGCGGCTGTTCCACGACTGGCTGGTCAACGCGGGTC
TGTAA

Upstream 100 bases:

>100_bases
GCCCCGCAACGGGAAGAGGTCGTCGCCGAGAGTTAGGGCATTCGGACTACGGCCTTTCTACCTTCTCGACATTGCAATTT
TTGCACCCCACATAGGGACC

Downstream 100 bases:

>100_bases
CCGCGGCCCCGGCCTCCGTGCTGCGCCTGCCCAGCCGGAACTCGACCGGCACACATGCCATCCACGGTATCAGGACGGGC
GAGACGAACATCAATCCGAT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MPLRRLPPLRALEAFMRTVRLGSARAAAEELNLSPSALSRRIANLEEFVGKKLFTRAKQAMGLTDEGHQFYEAVSPHFEA
LQRAVEGQSDNLSLLRLRLGVLPLFGSQRLFPRLGELRARHKLLHIDIDTGPHLEDRVGDTLDAAIILSRGPERGLHAVR
LDENKVHAICSKELAADIGTNPDQDTLAKQTFLIHNELPESFMAWKDELDMRDLDPAAIDHYDSGQLMLEAAAQGLGIAI
MHDDHLRRADDDRLTELFDLDVDSPYSYWFVCKPTALESRPVRLFHDWLVNAGL

Sequences:

>Translated_294_residues
MPLRRLPPLRALEAFMRTVRLGSARAAAEELNLSPSALSRRIANLEEFVGKKLFTRAKQAMGLTDEGHQFYEAVSPHFEA
LQRAVEGQSDNLSLLRLRLGVLPLFGSQRLFPRLGELRARHKLLHIDIDTGPHLEDRVGDTLDAAIILSRGPERGLHAVR
LDENKVHAICSKELAADIGTNPDQDTLAKQTFLIHNELPESFMAWKDELDMRDLDPAAIDHYDSGQLMLEAAAQGLGIAI
MHDDHLRRADDDRLTELFDLDVDSPYSYWFVCKPTALESRPVRLFHDWLVNAGL
>Mature_293_residues
PLRRLPPLRALEAFMRTVRLGSARAAAEELNLSPSALSRRIANLEEFVGKKLFTRAKQAMGLTDEGHQFYEAVSPHFEAL
QRAVEGQSDNLSLLRLRLGVLPLFGSQRLFPRLGELRARHKLLHIDIDTGPHLEDRVGDTLDAAIILSRGPERGLHAVRL
DENKVHAICSKELAADIGTNPDQDTLAKQTFLIHNELPESFMAWKDELDMRDLDPAAIDHYDSGQLMLEAAAQGLGIAIM
HDDHLRRADDDRLTELFDLDVDSPYSYWFVCKPTALESRPVRLFHDWLVNAGL

Specific function: Regulatory Protein For The Glycine Cleavage System Operon (Gcv). Mediates Activation Of Gcv By Glycine And Repression By Purines. Gcva Is Negatively Autoregulated. Bind To Three Sites Upstream Of The Gcv Promoter. [C]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=27.0833333333333, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786448, Length=235, Percent_Identity=27.2340425531915, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=168, Percent_Identity=31.547619047619, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=25.2, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33094; Mature: 32963

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRRLPPLRALEAFMRTVRLGSARAAAEELNLSPSALSRRIANLEEFVGKKLFTRAKQA
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLTDEGHQFYEAVSPHFEALQRAVEGQSDNLSLLRLRLGVLPLFGSQRLFPRLGELRAR
CCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
HKLLHIDIDTGPHLEDRVGDTLDAAIILSRGPERGLHAVRLDENKVHAICSKELAADIGT
CEEEEEECCCCCCHHHHHCCHHHHHHEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCC
NPDQDTLAKQTFLIHNELPESFMAWKDELDMRDLDPAAIDHYDSGQLMLEAAAQGLGIAI
CCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHCCCEEEE
MHDDHLRRADDDRLTELFDLDVDSPYSYWFVCKPTALESRPVRLFHDWLVNAGL
EECCHHCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PLRRLPPLRALEAFMRTVRLGSARAAAEELNLSPSALSRRIANLEEFVGKKLFTRAKQA
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLTDEGHQFYEAVSPHFEALQRAVEGQSDNLSLLRLRLGVLPLFGSQRLFPRLGELRAR
CCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
HKLLHIDIDTGPHLEDRVGDTLDAAIILSRGPERGLHAVRLDENKVHAICSKELAADIGT
CEEEEEECCCCCCHHHHHCCHHHHHHEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCC
NPDQDTLAKQTFLIHNELPESFMAWKDELDMRDLDPAAIDHYDSGQLMLEAAAQGLGIAI
CCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHCCCEEEE
MHDDHLRRADDDRLTELFDLDVDSPYSYWFVCKPTALESRPVRLFHDWLVNAGL
EECCHHCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]