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The map label for this gene is metZ [H]

Identifier: 85374485

GI number: 85374485

Start: 1702691

End: 1703908

Strand: Reverse

Name: metZ [H]

Synonym: ELI_08290

Alternate gene names: 85374485

Gene position: 1703908-1702691 (Counterclockwise)

Preceding gene: 85374486

Following gene: 85374482

Centisome position: 55.82

GC content: 66.09

Gene sequence:

>1218_bases
ATGAAGAAGACTACCGGCACCGACCGTTCCGTCACCCGCAACTGGCGCCCTGCCACCCAGGCGGTGCGCGGCGGCACCTG
GCGCAGCGAACACGGCGAGACCAGCGAAGCGCTGTTCCTCACCTCCGGCTACACCTACGACGATGCGCGGACCGTGGCGG
ACCGCTTCGCCGGCGATGCCGAGGGCATGACCTATTCGCGTCTGCAGAACCCCACCGTCGCCATGCTGGAAGAGCGCATC
GCGCTGATGGAAGGGGCCGAGGCCTGCCGCGCGCAGGCGAGCGGCATGGCGGCGATGACCGCCGCCCTGCTGTGCCAGCT
ATCGACCGGCGATCACATCGTTTCGGCCAAGGCCGCCTTCGGCTCGTGCCGCTGGCTGGTCGATCACTTGTGCCCGCGCT
TCGGGATCGAGACGACGGTTATCGACAGCGCCGACAATGATGCCTGGGAGGCCGCGATCCGGCCCAACACCAATGTCTTC
TTCTTCGAGACTCCCGCCAATCCCACGCTCGACGTGGTCGACATGCAGCATGTCTGCGACCTCGCCCGGGCGCACGGCAT
CACCACCGTGGTCGACAACGCTTTCGCTACCAGCGTGCTGCAAAAGCCGCTCGACTTCGGGGCCGACGTGATCGCCTACA
GCGCGACCAAGCTGATGGACGGGCAGGGCCGCGTGCTCGCCGGTGCGGTGTGCGGCAGCGAGGAGTGGATCAACGAAGTC
CTGTTGCCGTTCCAGCGCAACACCGGGCCGAACCTTTCGCCCTTCAATGCCTGGGTGGTGCACAAGGGGCTCGAAACGCT
CGAGCTGCGCGCCATGAAGCAGAGCGAGAATGCTGTCGCACTCGGCGAGTTCATGGAGGATCGGGTGCGCAAAGCCGGTG
GCACGATGCTCCACCCGGGCCTGCCTAGCCATCCGCGCCACGATCTCGCGATGAAGCAGATGCGCGCGACCGGCCCGATC
TTCGCTTTCGATGTCGGCAGCCGCTCCACCGCCTTCGCCGTGCTCGATGCGCTCGCGCTGGTCGATATCTCCAACAATAT
CGGCGATGCGCGCAGCCTCATGTGCCACCCGGCCAGCACGACCCACGCCAATATGGGCGCAGAAGCGCGTGCGGACATGG
GCGTGACCGAGGGTCTCCTCCGCATCAATGTGGGGCTGGAGGATATCGAGGATCTGAGGGAAGATCTCGACCAAGCGTTC
GCGGCGGCCGGTCTTTAG

Upstream 100 bases:

>100_bases
GCGCGTCGTGACTAGCCCGCGCTTCGCTGCAACCTGCCGTTGCCGCACCTAAAGCGCGCGCCGCTTGCCGCCCGCCCGCT
CGCTGCGTAAGCGGCATGGC

Downstream 100 bases:

>100_bases
CGCGCGGACAGCATCCGCCTCTCCAGCACGTCGATCTCCTCCCGCAGGGCAAGCGCGACGGTCAGTGCGCGCCCGCAATC
GTCATTGTCGGTCAGGGCTG

Product: cystathionine beta-lyase

Products: methanethiol; NH3; 2-oxobutanoate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKKTTGTDRSVTRNWRPATQAVRGGTWRSEHGETSEALFLTSGYTYDDARTVADRFAGDAEGMTYSRLQNPTVAMLEERI
ALMEGAEACRAQASGMAAMTAALLCQLSTGDHIVSAKAAFGSCRWLVDHLCPRFGIETTVIDSADNDAWEAAIRPNTNVF
FFETPANPTLDVVDMQHVCDLARAHGITTVVDNAFATSVLQKPLDFGADVIAYSATKLMDGQGRVLAGAVCGSEEWINEV
LLPFQRNTGPNLSPFNAWVVHKGLETLELRAMKQSENAVALGEFMEDRVRKAGGTMLHPGLPSHPRHDLAMKQMRATGPI
FAFDVGSRSTAFAVLDALALVDISNNIGDARSLMCHPASTTHANMGAEARADMGVTEGLLRINVGLEDIEDLREDLDQAF
AAAGL

Sequences:

>Translated_405_residues
MKKTTGTDRSVTRNWRPATQAVRGGTWRSEHGETSEALFLTSGYTYDDARTVADRFAGDAEGMTYSRLQNPTVAMLEERI
ALMEGAEACRAQASGMAAMTAALLCQLSTGDHIVSAKAAFGSCRWLVDHLCPRFGIETTVIDSADNDAWEAAIRPNTNVF
FFETPANPTLDVVDMQHVCDLARAHGITTVVDNAFATSVLQKPLDFGADVIAYSATKLMDGQGRVLAGAVCGSEEWINEV
LLPFQRNTGPNLSPFNAWVVHKGLETLELRAMKQSENAVALGEFMEDRVRKAGGTMLHPGLPSHPRHDLAMKQMRATGPI
FAFDVGSRSTAFAVLDALALVDISNNIGDARSLMCHPASTTHANMGAEARADMGVTEGLLRINVGLEDIEDLREDLDQAF
AAAGL
>Mature_405_residues
MKKTTGTDRSVTRNWRPATQAVRGGTWRSEHGETSEALFLTSGYTYDDARTVADRFAGDAEGMTYSRLQNPTVAMLEERI
ALMEGAEACRAQASGMAAMTAALLCQLSTGDHIVSAKAAFGSCRWLVDHLCPRFGIETTVIDSADNDAWEAAIRPNTNVF
FFETPANPTLDVVDMQHVCDLARAHGITTVVDNAFATSVLQKPLDFGADVIAYSATKLMDGQGRVLAGAVCGSEEWINEV
LLPFQRNTGPNLSPFNAWVVHKGLETLELRAMKQSENAVALGEFMEDRVRKAGGTMLHPGLPSHPRHDLAMKQMRATGPI
FAFDVGSRSTAFAVLDALALVDISNNIGDARSLMCHPASTTHANMGAEARADMGVTEGLLRINVGLEDIEDLREDLDQAF
AAAGL

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=350, Percent_Identity=37.1428571428571, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI299473759, Length=350, Percent_Identity=34.2857142857143, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI25453487, Length=349, Percent_Identity=32.378223495702, Blast_Score=145, Evalue=9e-35,
Organism=Escherichia coli, GI1790375, Length=392, Percent_Identity=32.6530612244898, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=25.765306122449, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538055, Length=370, Percent_Identity=36.4864864864865, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17560056, Length=350, Percent_Identity=36.2857142857143, Blast_Score=214, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=34.2318059299191, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=393, Percent_Identity=33.587786259542, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=416, Percent_Identity=29.8076923076923, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=413, Percent_Identity=26.1501210653753, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=270, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=342, Percent_Identity=26.9005847953216, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922554, Length=353, Percent_Identity=36.5439093484419, Blast_Score=231, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 4.4.1.11

Molecular weight: Translated: 43691; Mature: 43691

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTTGTDRSVTRNWRPATQAVRGGTWRSEHGETSEALFLTSGYTYDDARTVADRFAGDA
CCCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCC
EGMTYSRLQNPTVAMLEERIALMEGAEACRAQASGMAAMTAALLCQLSTGDHIVSAKAAF
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEHHHHH
GSCRWLVDHLCPRFGIETTVIDSADNDAWEAAIRPNTNVFFFETPANPTLDVVDMQHVCD
HHHHHHHHHHCHHCCCCEEEEECCCCCCEEEEECCCCCEEEEECCCCCCEEHHHHHHHHH
LARAHGITTVVDNAFATSVLQKPLDFGADVIAYSATKLMDGQGRVLAGAVCGSEEWINEV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
LLPFQRNTGPNLSPFNAWVVHKGLETLELRAMKQSENAVALGEFMEDRVRKAGGTMLHPG
HCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCC
LPSHPRHDLAMKQMRATGPIFAFDVGSRSTAFAVLDALALVDISNNIGDARSLMCHPAST
CCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCC
THANMGAEARADMGVTEGLLRINVGLEDIEDLREDLDQAFAAAGL
CCCCCCCCHHHCCCHHCCEEEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKTTGTDRSVTRNWRPATQAVRGGTWRSEHGETSEALFLTSGYTYDDARTVADRFAGDA
CCCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCC
EGMTYSRLQNPTVAMLEERIALMEGAEACRAQASGMAAMTAALLCQLSTGDHIVSAKAAF
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEHHHHH
GSCRWLVDHLCPRFGIETTVIDSADNDAWEAAIRPNTNVFFFETPANPTLDVVDMQHVCD
HHHHHHHHHHCHHCCCCEEEEECCCCCCEEEEECCCCCEEEEECCCCCCEEHHHHHHHHH
LARAHGITTVVDNAFATSVLQKPLDFGADVIAYSATKLMDGQGRVLAGAVCGSEEWINEV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
LLPFQRNTGPNLSPFNAWVVHKGLETLELRAMKQSENAVALGEFMEDRVRKAGGTMLHPG
HCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCC
LPSHPRHDLAMKQMRATGPIFAFDVGSRSTAFAVLDALALVDISNNIGDARSLMCHPAST
CCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCC
THANMGAEARADMGVTEGLLRINVGLEDIEDLREDLDQAFAAAGL
CCCCCCCCHHHCCCHHCCEEEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-methionine

Specific reaction: L-methionine = methanethiol + NH3 + 2-oxobutanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]