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The map label for this gene is lpd3 [H]

Identifier: 85374429

GI number: 85374429

Start: 1661026

End: 1662444

Strand: Direct

Name: lpd3 [H]

Synonym: ELI_08010

Alternate gene names: 85374429

Gene position: 1661026-1662444 (Clockwise)

Preceding gene: 85374428

Following gene: 85374430

Centisome position: 54.42

GC content: 62.02

Gene sequence:

>1419_bases
ATGGCTGACCATTCCTTTGATTACGACGTCCTCGTTATCGGCGCTGGTCCGGGTGGCTATGTCGCCGCCATCCGCGCTGC
GCAGCTGGGGCTCAAGACCGCTTGTGCCGAAGGCCGCGAAACACTCGGGGGCACCTGCCTCAATGTCGGCTGCATCCCGT
CCAAGGCGATGCTGCATGCCTCGGAATATTTCGATGCGGCCGCCAACGGCACTATGGAAAGCATGGGCATCGAGGTGAAG
CCCAAGCTCAATCTCGACAAGATGCACGGCCAGCGGCTCGATGCCGTCGACGGGCTGACCAAGGGCATCGAATTCCTGTT
CAAGAAGAACAAGGTCGACTGGAAAAAGGGTTACGCCACCTTCCAGGACGCCCACACTGTCAAAATCGGTGACGAGACCG
TCACCGCGAAAGACATCGTGATCGCGACCGGCTCTTCCGTCACCCCCCTGCCCGGGGTCGAGGTCGACAATGCCAAGGGC
GTGGTGGTCGATTCCACCGGCGCGCTCGAATTGGCCAAGGTGCCCAGAAAAATGGTCGTGATCGGCGGCGGCGTGATCGG
GCTGGAACTCGGCTCGGTCTGGCGGCGGCTAGGCGCTGAAGTGACGTGCGTCGAGTTTCTCGATGAAATCCTGCCCGGTA
TGGACGGCGATATCCGCAAGGAGTCGCGCAAGATTTTCAAGAAGCAGGGGATCGAGTTCAAGCTGAAGACCAAGGTCACG
GGCGTAACTGTCAAGGGCAAGAAAGCCCATCTGACGCTAGAACCTGCCGACGGAGGCGACGAAGAGACGATGGAGGCCGA
TTGCGTGCTCGTCTCCATCGGTCGCAGGCCCAACACCGAAGGGCTCGGCCTCGACTCTATCGGCCTCGACGTGAACAAGC
GCGGCCAGATCGAAACCGATCACGATTTCCGCACCAGTGTGGAAGGCGTCTGGGCCATCGGCGACGTCGTTCCCGGTCCG
ATGCTGGCCCACAAGGCGGAAGACGAAGGCATCGCGGTGGCGGAGAACATCGCCGGGCAGACCGGCATCGTGAACCACGC
CGTCATCCCCAGCGTCGTCTATACCCTGCCCGAGATCGCTGGCGTCGGCCTGACCACCGAACAAGCCATCGAAGCCGCTG
GCGGCGACAAGACCAAGGTGAAAGTCGGCAAATTCCCGATGATGGCCAACAGCCGCGCCAAGACCAATCACGAGCCCGAC
GGCTTGGTGAAGGTCATCGCCGACGCCGAGACCGACCGCGTTCTGGGCATATGGGCCATCGCCAGCGTCGCCGGCACGAT
GATAGCCGAAGCGGGCATCGCGATGGAATTCGGCGCAACGAGCGAGGACATTGCCTACACCTGCCACGCCCACCCGACCC
ACGCCGAAGCGATGAAGGAAGCGGCGATGGCAGTTCAGGGCAAGCCGATCCACATTTGA

Upstream 100 bases:

>100_bases
CCGTTCGGGCTGAGCCTGTCGAAGCCCTGTCCTTCTTCTGGACCGACGGAAAAAAGGAAGTACAGCCCTTCGACAGGCTC
AGGGCGAACGGAGTTTGGAA

Downstream 100 bases:

>100_bases
TCGACTGGTCTGCCCATGCCTCCCTTCCACCTCGCCTTTCCCGTCCATGACCTGATCGCTGCGCGCGAGTTCTGGGGTGG
GGTCATGGGTTGTGCGGAAG

Product: 2-oxoglutarate dehydrogenase, E3 component, lipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MADHSFDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRETLGGTCLNVGCIPSKAMLHASEYFDAAANGTMESMGIEVK
PKLNLDKMHGQRLDAVDGLTKGIEFLFKKNKVDWKKGYATFQDAHTVKIGDETVTAKDIVIATGSSVTPLPGVEVDNAKG
VVVDSTGALELAKVPRKMVVIGGGVIGLELGSVWRRLGAEVTCVEFLDEILPGMDGDIRKESRKIFKKQGIEFKLKTKVT
GVTVKGKKAHLTLEPADGGDEETMEADCVLVSIGRRPNTEGLGLDSIGLDVNKRGQIETDHDFRTSVEGVWAIGDVVPGP
MLAHKAEDEGIAVAENIAGQTGIVNHAVIPSVVYTLPEIAGVGLTTEQAIEAAGGDKTKVKVGKFPMMANSRAKTNHEPD
GLVKVIADAETDRVLGIWAIASVAGTMIAEAGIAMEFGATSEDIAYTCHAHPTHAEAMKEAAMAVQGKPIHI

Sequences:

>Translated_472_residues
MADHSFDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRETLGGTCLNVGCIPSKAMLHASEYFDAAANGTMESMGIEVK
PKLNLDKMHGQRLDAVDGLTKGIEFLFKKNKVDWKKGYATFQDAHTVKIGDETVTAKDIVIATGSSVTPLPGVEVDNAKG
VVVDSTGALELAKVPRKMVVIGGGVIGLELGSVWRRLGAEVTCVEFLDEILPGMDGDIRKESRKIFKKQGIEFKLKTKVT
GVTVKGKKAHLTLEPADGGDEETMEADCVLVSIGRRPNTEGLGLDSIGLDVNKRGQIETDHDFRTSVEGVWAIGDVVPGP
MLAHKAEDEGIAVAENIAGQTGIVNHAVIPSVVYTLPEIAGVGLTTEQAIEAAGGDKTKVKVGKFPMMANSRAKTNHEPD
GLVKVIADAETDRVLGIWAIASVAGTMIAEAGIAMEFGATSEDIAYTCHAHPTHAEAMKEAAMAVQGKPIHI
>Mature_471_residues
ADHSFDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRETLGGTCLNVGCIPSKAMLHASEYFDAAANGTMESMGIEVKP
KLNLDKMHGQRLDAVDGLTKGIEFLFKKNKVDWKKGYATFQDAHTVKIGDETVTAKDIVIATGSSVTPLPGVEVDNAKGV
VVDSTGALELAKVPRKMVVIGGGVIGLELGSVWRRLGAEVTCVEFLDEILPGMDGDIRKESRKIFKKQGIEFKLKTKVTG
VTVKGKKAHLTLEPADGGDEETMEADCVLVSIGRRPNTEGLGLDSIGLDVNKRGQIETDHDFRTSVEGVWAIGDVVPGPM
LAHKAEDEGIAVAENIAGQTGIVNHAVIPSVVYTLPEIAGVGLTTEQAIEAAGGDKTKVKVGKFPMMANSRAKTNHEPDG
LVKVIADAETDRVLGIWAIASVAGTMIAEAGIAMEFGATSEDIAYTCHAHPTHAEAMKEAAMAVQGKPIHI

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=480, Percent_Identity=52.2916666666667, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=28.997867803838, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI148277065, Length=477, Percent_Identity=28.3018867924528, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519430, Length=477, Percent_Identity=28.3018867924528, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519428, Length=477, Percent_Identity=28.3018867924528, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519426, Length=477, Percent_Identity=28.3018867924528, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148277071, Length=477, Percent_Identity=28.3018867924528, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI291045266, Length=478, Percent_Identity=28.0334728033473, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=29.4491525423729, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI291045268, Length=476, Percent_Identity=26.4705882352941, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=39.9568034557235, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=32.3394495412844, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=30.5439330543933, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI87081717, Length=470, Percent_Identity=27.2340425531915, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789065, Length=248, Percent_Identity=27.8225806451613, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=472, Percent_Identity=51.6949152542373, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=29.3032786885246, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=476, Percent_Identity=29.2016806722689, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=476, Percent_Identity=29.2016806722689, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=488, Percent_Identity=27.2540983606557, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=227, Percent_Identity=28.6343612334802, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=48.8517745302714, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6325240, Length=496, Percent_Identity=34.0725806451613, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6325166, Length=479, Percent_Identity=29.4363256784969, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21358499, Length=476, Percent_Identity=51.6806722689076, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24640549, Length=481, Percent_Identity=29.5218295218295, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640553, Length=481, Percent_Identity=29.5218295218295, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640551, Length=481, Percent_Identity=29.5218295218295, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17737741, Length=489, Percent_Identity=28.8343558282209, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49729; Mature: 49597

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADHSFDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRETLGGTCLNVGCIPSKAMLHA
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHHH
SEYFDAAANGTMESMGIEVKPKLNLDKMHGQRLDAVDGLTKGIEFLFKKNKVDWKKGYAT
HHHHHHHCCCCHHHCCEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCE
FQDAHTVKIGDETVTAKDIVIATGSSVTPLPGVEVDNAKGVVVDSTGALELAKVPRKMVV
ECCCEEEEECCCEEEECEEEEECCCCCCCCCCCEECCCCEEEEECCCCEEHHHCCCEEEE
IGGGVIGLELGSVWRRLGAEVTCVEFLDEILPGMDGDIRKESRKIFKKQGIEFKLKTKVT
EECCEEEEHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEE
GVTVKGKKAHLTLEPADGGDEETMEADCVLVSIGRRPNTEGLGLDSIGLDVNKRGQIETD
EEEEECCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HDFRTSVEGVWAIGDVVPGPMLAHKAEDEGIAVAENIAGQTGIVNHAVIPSVVYTLPEIA
CCHHHCCCCEEEECCCCCCCHHEECCCCCCCEEEHHCCCCCCCCHHHHHHHHHHHHHHHH
GVGLTTEQAIEAAGGDKTKVKVGKFPMMANSRAKTNHEPDGLVKVIADAETDRVLGIWAI
CCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEHHHH
ASVAGTMIAEAGIAMEFGATSEDIAYTCHAHPTHAEAMKEAAMAVQGKPIHI
HHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADHSFDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRETLGGTCLNVGCIPSKAMLHA
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHHH
SEYFDAAANGTMESMGIEVKPKLNLDKMHGQRLDAVDGLTKGIEFLFKKNKVDWKKGYAT
HHHHHHHCCCCHHHCCEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCE
FQDAHTVKIGDETVTAKDIVIATGSSVTPLPGVEVDNAKGVVVDSTGALELAKVPRKMVV
ECCCEEEEECCCEEEECEEEEECCCCCCCCCCCEECCCCEEEEECCCCEEHHHCCCEEEE
IGGGVIGLELGSVWRRLGAEVTCVEFLDEILPGMDGDIRKESRKIFKKQGIEFKLKTKVT
EECCEEEEHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEE
GVTVKGKKAHLTLEPADGGDEETMEADCVLVSIGRRPNTEGLGLDSIGLDVNKRGQIETD
EEEEECCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HDFRTSVEGVWAIGDVVPGPMLAHKAEDEGIAVAENIAGQTGIVNHAVIPSVVYTLPEIA
CCHHHCCCCEEEECCCCCCCHHEECCCCCCCEEEHHCCCCCCCCHHHHHHHHHHHHHHHH
GVGLTTEQAIEAAGGDKTKVKVGKFPMMANSRAKTNHEPDGLVKVIADAETDRVLGIWAI
CCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEHHHH
ASVAGTMIAEAGIAMEFGATSEDIAYTCHAHPTHAEAMKEAAMAVQGKPIHI
HHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]