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The map label for this gene is sucB [H]

Identifier: 85374428

GI number: 85374428

Start: 1659659

End: 1660909

Strand: Direct

Name: sucB [H]

Synonym: ELI_08005

Alternate gene names: 85374428

Gene position: 1659659-1660909 (Clockwise)

Preceding gene: 85374427

Following gene: 85374429

Centisome position: 54.37

GC content: 64.59

Gene sequence:

>1251_bases
ATGACGACAGAAATCCAGGTCCCCCAGCTCGGTGAATCGGTCACCGAAGGCACGATCGGCGAATGGCTCAAGCAGCCCGG
CGATGCGGTCGAGGTCGACGAGCCCATCGCCAGCCTCGAGACCGACAAGGTCGCGGTCGAGGTGCCTTCCCCGGTCGCGG
GCGTGATCGGCGAGCTCAAGGCCGAGGTTGGCGACACCGTCGAAGTCGGCGCCGTGATCGCAACGGTCGAAGAAGGCGCG
ACCGGCGCTGCGACCAAGGGTGAAGAGCCCGCCCGCTCGCAGGAAAAACGCGAGCAAGGCCGGGAAGAACGCGCCGAGCA
GGAAGAAGCGACCGATTCCCCCTCTGTCGACGGATCGCAGACGCTCAGCCCGGCCGTGCGCCGCGCAGTGCTGGAACACG
GGGTCGATCCCTCGACCATCAAGGGCACCGGCAAGGACGGGCGCCTGACCAAGGAAGACGTGGTCGCCGCGGCGCGCGCC
AAGCGCGATGGCGGCGGCGAGAGCGCCAGCGCTCCCGCCCCAGCCCCGGCCGCAGCCACTTCCGGCGGCGAACGCCGCGA
GGAGCGCGTCAAGATGACGCGCATGCGCCAGACCATCGCCAAGCGTTTGAAGGGCGCGCAGGAAGAGGCCGCGCTGCTCA
CGACCTTCAATGATGTCGACATGTCCGCGGTGATCGAGGCACGCACGAAGTACAAGGATTTGTTCGCCAAGAAGCACGAC
ATCCGCTTGGGCTTCATGGGCTTCTTCGCCAAAGCCGCGTGCCTTGCCCTGAAGGACGTGCCGAGCGTCAACGCCTATAT
CGAAGGCGAAGAGATCGTCTATCACGACTACATCGATATTTCCGTCGCCGTCAGCGCGCCCAATGGCCTGGTGGTTCCGG
TGATCCGCGACGCGCAGGCCAAGGGTTTCGCTCAGATCGAGAAAGACATCGCCGATTTCGGCAAGCGCGCAAAGGAAGGC
ACGCTGACGATGGAAGACATGAAGGGCGGCACCTTCACCATCTCCAACGGCGGCGTGTTCGGCAGCCTGATGTCGACCCC
GATCATCAACCCGCCGCAAAGCGCGGTGCTGGGCCTGCATCGCATTGAAGACCGTCCGGTTGCCGTGAATGGCGAAGTCG
TCATCCGCCCGATGATGTACATCGCGCTGAGTTACGACCACCGCCTGATCGACGGCCGCGAAGCCGTCACCGCACTCAAG
ATCATCAAGGAAGCGATCGAAGATCCCACCCGGATGCTGATCGACCTCTAG

Upstream 100 bases:

>100_bases
AGCTTGGTGTGCATCGCTCTCGACCTCGCCAATTGCGGCGAGACCGCCAAGGTCACCCTGAAACGCCCGCCGAACAAGCC
AAAGGGCTGAGGAAGAACAC

Downstream 100 bases:

>100_bases
AGATCAACCCCCATGTCCGTTCGGGCTGAGCCTGTCGAAGCCCTGTCCTTCTTCTGGACCGACGGAAAAAAGGAAGTACA
GCCCTTCGACAGGCTCAGGG

Product: dihydrolipoamide succinyl transferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTTEIQVPQLGESVTEGTIGEWLKQPGDAVEVDEPIASLETDKVAVEVPSPVAGVIGELKAEVGDTVEVGAVIATVEEGA
TGAATKGEEPARSQEKREQGREERAEQEEATDSPSVDGSQTLSPAVRRAVLEHGVDPSTIKGTGKDGRLTKEDVVAAARA
KRDGGGESASAPAPAPAAATSGGERREERVKMTRMRQTIAKRLKGAQEEAALLTTFNDVDMSAVIEARTKYKDLFAKKHD
IRLGFMGFFAKAACLALKDVPSVNAYIEGEEIVYHDYIDISVAVSAPNGLVVPVIRDAQAKGFAQIEKDIADFGKRAKEG
TLTMEDMKGGTFTISNGGVFGSLMSTPIINPPQSAVLGLHRIEDRPVAVNGEVVIRPMMYIALSYDHRLIDGREAVTALK
IIKEAIEDPTRMLIDL

Sequences:

>Translated_416_residues
MTTEIQVPQLGESVTEGTIGEWLKQPGDAVEVDEPIASLETDKVAVEVPSPVAGVIGELKAEVGDTVEVGAVIATVEEGA
TGAATKGEEPARSQEKREQGREERAEQEEATDSPSVDGSQTLSPAVRRAVLEHGVDPSTIKGTGKDGRLTKEDVVAAARA
KRDGGGESASAPAPAPAAATSGGERREERVKMTRMRQTIAKRLKGAQEEAALLTTFNDVDMSAVIEARTKYKDLFAKKHD
IRLGFMGFFAKAACLALKDVPSVNAYIEGEEIVYHDYIDISVAVSAPNGLVVPVIRDAQAKGFAQIEKDIADFGKRAKEG
TLTMEDMKGGTFTISNGGVFGSLMSTPIINPPQSAVLGLHRIEDRPVAVNGEVVIRPMMYIALSYDHRLIDGREAVTALK
IIKEAIEDPTRMLIDL
>Mature_415_residues
TTEIQVPQLGESVTEGTIGEWLKQPGDAVEVDEPIASLETDKVAVEVPSPVAGVIGELKAEVGDTVEVGAVIATVEEGAT
GAATKGEEPARSQEKREQGREERAEQEEATDSPSVDGSQTLSPAVRRAVLEHGVDPSTIKGTGKDGRLTKEDVVAAARAK
RDGGGESASAPAPAPAAATSGGERREERVKMTRMRQTIAKRLKGAQEEAALLTTFNDVDMSAVIEARTKYKDLFAKKHDI
RLGFMGFFAKAACLALKDVPSVNAYIEGEEIVYHDYIDISVAVSAPNGLVVPVIRDAQAKGFAQIEKDIADFGKRAKEGT
LTMEDMKGGTFTISNGGVFGSLMSTPIINPPQSAVLGLHRIEDRPVAVNGEVVIRPMMYIALSYDHRLIDGREAVTALKI
IKEAIEDPTRMLIDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=60.2362204724409, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=29.490022172949, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=29.490022172949, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=28.7671232876712, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=27.9176201372998, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=37.5, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786946, Length=418, Percent_Identity=49.0430622009569, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1786305, Length=437, Percent_Identity=32.4942791762014, Blast_Score=195, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI25146366, Length=416, Percent_Identity=45.9134615384615, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=29.2517006802721, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=429, Percent_Identity=28.2051282051282, Blast_Score=167, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=30.4918032786885, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=46.9194312796209, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=28.7610619469027, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=236, Percent_Identity=60.5932203389831, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=28.1395348837209, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44416; Mature: 44285

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEIQVPQLGESVTEGTIGEWLKQPGDAVEVDEPIASLETDKVAVEVPSPVAGVIGELK
CCCCCCCCHHCCHHHCCHHHHHHHCCCCCEECCCHHHHCCCCCEEEECCCCHHHHHHHHH
AEVGDTVEVGAVIATVEEGATGAATKGEEPARSQEKREQGREERAEQEEATDSPSVDGSQ
HHCCCCEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
TLSPAVRRAVLEHGVDPSTIKGTGKDGRLTKEDVVAAARAKRDGGGESASAPAPAPAAAT
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
SGGERREERVKMTRMRQTIAKRLKGAQEEAALLTTFNDVDMSAVIEARTKYKDLFAKKHD
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHC
IRLGFMGFFAKAACLALKDVPSVNAYIEGEEIVYHDYIDISVAVSAPNGLVVPVIRDAQA
CEEHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEEEEEEEEECCCCEEEEEECCCCC
KGFAQIEKDIADFGKRAKEGTLTMEDMKGGTFTISNGGVFGSLMSTPIINPPQSAVLGLH
CHHHHHHHHHHHHHHHHCCCCEEHEECCCCEEEEECCCEEHHHHHCCCCCCCHHHHHHHH
RIEDRPVAVNGEVVIRPMMYIALSYDHRLIDGREAVTALKIIKEAIEDPTRMLIDL
HCCCCCEEECCCEEEEEHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
TTEIQVPQLGESVTEGTIGEWLKQPGDAVEVDEPIASLETDKVAVEVPSPVAGVIGELK
CCCCCCCHHCCHHHCCHHHHHHHCCCCCEECCCHHHHCCCCCEEEECCCCHHHHHHHHH
AEVGDTVEVGAVIATVEEGATGAATKGEEPARSQEKREQGREERAEQEEATDSPSVDGSQ
HHCCCCEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
TLSPAVRRAVLEHGVDPSTIKGTGKDGRLTKEDVVAAARAKRDGGGESASAPAPAPAAAT
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
SGGERREERVKMTRMRQTIAKRLKGAQEEAALLTTFNDVDMSAVIEARTKYKDLFAKKHD
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHC
IRLGFMGFFAKAACLALKDVPSVNAYIEGEEIVYHDYIDISVAVSAPNGLVVPVIRDAQA
CEEHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEEEEEEEEECCCCEEEEEECCCCC
KGFAQIEKDIADFGKRAKEGTLTMEDMKGGTFTISNGGVFGSLMSTPIINPPQSAVLGLH
CHHHHHHHHHHHHHHHHCCCCEEHEECCCCEEEEECCCEEHHHHHCCCCCCCHHHHHHHH
RIEDRPVAVNGEVVIRPMMYIALSYDHRLIDGREAVTALKIIKEAIEDPTRMLIDL
HCCCCCEEECCCEEEEEHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]