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The map label for this gene is mdh [H]

Identifier: 85374423

GI number: 85374423

Start: 1653975

End: 1654949

Strand: Direct

Name: mdh [H]

Synonym: ELI_07980

Alternate gene names: 85374423

Gene position: 1653975-1654949 (Clockwise)

Preceding gene: 85374422

Following gene: 85374424

Centisome position: 54.19

GC content: 62.87

Gene sequence:

>975_bases
ATGGCCCACGCGACTGGACGCAAGAAGATCGCCCTCATCGGCTCCGGCATGATCGGCGGCACCCTCGCCCACCTCGCCGC
GAAGAAGGAAATGGGCGACATCGTCCTGTTCGACATCGCCGAAGGCATGCCCCAGGGCAAGGCGCTGGACCTCAGCCAGT
GCGGCCCGATCGAGGGCTTCGACGCCAAGATCACCGGTTCGAACGACTATGCCGATATCGCTGGCGCCGATGTCGTGATC
GTCACCGCCGGCGTTCCGCGCAAGCCGGGCATGAGCCGCGACGATCTGCTCGGCATCAACCTGAAAGTGATGAAGGCGGT
CGGCGAAGGCATCAAGAACAACTGCCCCGACGCATTCGTGATCTGCATCACCAACCCGCTCGACGCGATGGTCTGGGCGC
TGCGCGAATTCTCCGGCTTGCCGCACAACATGGTGGTCGGCATGGCAGGCGTGCTCGACAGCGCGCGTTTCGCGACCTTC
CTCGCCTGGGAATTCGGCGTTTCGACCCGTGACGTGAACGCCTTCGTGCTCGGCGGCCACGGCGACACGATGGTCCCGGT
GAAAAGCTATACCACGATCAACGGCATTCCGGTCGACGACTACGCCAAGATCAAGGGCGTGAGCGAAGACCGCATCGACG
AAATCGTCGACCGTACCCGCAAAGGTGGCGGCGAGATCGTCGGCCTGCTCGGCAACGGCTCGGCCTATTACGCCCCCGCC
ACCAGCGCGATCGCAATGGCCGAAGCCTATCTCGGCGACCAGAAGCGCATCCTGCCGTGTGCAAGCTATGTCGAAGGCAA
GTATGGCCTCGACGGGCTGTACGTCGGCGTCCCCACCGTGATCGGCGCGGGCGGCACCGAGGAAGTGATCGAGATCGAGC
TGTCGGACGAAGAGAAGAGCAATCTCAAGGTTTCGACCGACGCGGTTGAAGAGTTGCTTGAGGCTTGTAAGGGTCTGGAT
AGCTCGCTTGTCTAA

Upstream 100 bases:

>100_bases
GGCTCCCGAGATTCCGGGCGCGGCTTCCTCGCGGGACGCGGGCGTGCGCCATTCGGAGAGGCTTGACCGGTCCCGCGCCC
TGGAAGGACTGAATCCCACT

Downstream 100 bases:

>100_bases
AGCCCTGCCAATCCTGACCCACTCGCTTGCCGCGCTAACGCTGGCCCTATCGGCTAGCTCGGCAGCGCTTGGCCAAGAGA
AAACTTTCATTGAAGCGACA

Product: malate dehydrogenase, NAD-dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAHATGRKKIALIGSGMIGGTLAHLAAKKEMGDIVLFDIAEGMPQGKALDLSQCGPIEGFDAKITGSNDYADIAGADVVI
VTAGVPRKPGMSRDDLLGINLKVMKAVGEGIKNNCPDAFVICITNPLDAMVWALREFSGLPHNMVVGMAGVLDSARFATF
LAWEFGVSTRDVNAFVLGGHGDTMVPVKSYTTINGIPVDDYAKIKGVSEDRIDEIVDRTRKGGGEIVGLLGNGSAYYAPA
TSAIAMAEAYLGDQKRILPCASYVEGKYGLDGLYVGVPTVIGAGGTEEVIEIELSDEEKSNLKVSTDAVEELLEACKGLD
SSLV

Sequences:

>Translated_324_residues
MAHATGRKKIALIGSGMIGGTLAHLAAKKEMGDIVLFDIAEGMPQGKALDLSQCGPIEGFDAKITGSNDYADIAGADVVI
VTAGVPRKPGMSRDDLLGINLKVMKAVGEGIKNNCPDAFVICITNPLDAMVWALREFSGLPHNMVVGMAGVLDSARFATF
LAWEFGVSTRDVNAFVLGGHGDTMVPVKSYTTINGIPVDDYAKIKGVSEDRIDEIVDRTRKGGGEIVGLLGNGSAYYAPA
TSAIAMAEAYLGDQKRILPCASYVEGKYGLDGLYVGVPTVIGAGGTEEVIEIELSDEEKSNLKVSTDAVEELLEACKGLD
SSLV
>Mature_323_residues
AHATGRKKIALIGSGMIGGTLAHLAAKKEMGDIVLFDIAEGMPQGKALDLSQCGPIEGFDAKITGSNDYADIAGADVVIV
TAGVPRKPGMSRDDLLGINLKVMKAVGEGIKNNCPDAFVICITNPLDAMVWALREFSGLPHNMVVGMAGVLDSARFATFL
AWEFGVSTRDVNAFVLGGHGDTMVPVKSYTTINGIPVDDYAKIKGVSEDRIDEIVDRTRKGGGEIVGLLGNGSAYYAPAT
SAIAMAEAYLGDQKRILPCASYVEGKYGLDGLYVGVPTVIGAGGTEEVIEIELSDEEKSNLKVSTDAVEELLEACKGLDS
SLV

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=318, Percent_Identity=29.874213836478, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI221136809, Length=318, Percent_Identity=29.874213836478, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5031857, Length=309, Percent_Identity=29.126213592233, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI260099723, Length=309, Percent_Identity=29.126213592233, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI15082234, Length=313, Percent_Identity=28.4345047923323, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI291575128, Length=315, Percent_Identity=30.1587301587302, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI4557032, Length=315, Percent_Identity=30.1587301587302, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=29.8360655737705, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=29.8360655737705, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI260099725, Length=208, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI260099727, Length=208, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI21735621, Length=331, Percent_Identity=29.9093655589124, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI207028494, Length=190, Percent_Identity=28.4210526315789, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1789632, Length=323, Percent_Identity=32.1981424148607, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=26.3698630136986, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17554310, Length=306, Percent_Identity=31.0457516339869, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17561064, Length=303, Percent_Identity=24.4224422442244, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322765, Length=300, Percent_Identity=28.3333333333333, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324446, Length=261, Percent_Identity=29.8850574712644, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320125, Length=196, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136226, Length=308, Percent_Identity=29.2207792207792, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24647881, Length=317, Percent_Identity=31.2302839116719, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=27.7955271565495, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24663595, Length=296, Percent_Identity=31.4189189189189, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24663599, Length=314, Percent_Identity=29.9363057324841, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33975; Mature: 33844

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHATGRKKIALIGSGMIGGTLAHLAAKKEMGDIVLFDIAEGMPQGKALDLSQCGPIEGF
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECHHCCCCCCCC
DAKITGSNDYADIAGADVVIVTAGVPRKPGMSRDDLLGINLKVMKAVGEGIKNNCPDAFV
CEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCEEE
ICITNPLDAMVWALREFSGLPHNMVVGMAGVLDSARFATFLAWEFGVSTRDVNAFVLGGH
EEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCCEEEEECCC
GDTMVPVKSYTTINGIPVDDYAKIKGVSEDRIDEIVDRTRKGGGEIVGLLGNGSAYYAPA
CCEEEEEECCEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCEEECCC
TSAIAMAEAYLGDQKRILPCASYVEGKYGLDGLYVGVPTVIGAGGTEEVIEIELSDEEKS
HHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCEEEECCCCEECCCCCCEEEEEEECCCCCC
NLKVSTDAVEELLEACKGLDSSLV
CEEEEHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AHATGRKKIALIGSGMIGGTLAHLAAKKEMGDIVLFDIAEGMPQGKALDLSQCGPIEGF
CCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECHHCCCCCCCC
DAKITGSNDYADIAGADVVIVTAGVPRKPGMSRDDLLGINLKVMKAVGEGIKNNCPDAFV
CEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCEEE
ICITNPLDAMVWALREFSGLPHNMVVGMAGVLDSARFATFLAWEFGVSTRDVNAFVLGGH
EEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCCEEEEECCC
GDTMVPVKSYTTINGIPVDDYAKIKGVSEDRIDEIVDRTRKGGGEIVGLLGNGSAYYAPA
CCEEEEEECCEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCEEECCC
TSAIAMAEAYLGDQKRILPCASYVEGKYGLDGLYVGVPTVIGAGGTEEVIEIELSDEEKS
HHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCEEEECCCCEECCCCCCEEEEEEECCCCCC
NLKVSTDAVEELLEACKGLDSSLV
CEEEEHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA