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The map label for this gene is ligA

Identifier: 85374270

GI number: 85374270

Start: 1490451

End: 1492661

Strand: Reverse

Name: ligA

Synonym: ELI_07215

Alternate gene names: 85374270

Gene position: 1492661-1490451 (Counterclockwise)

Preceding gene: 85374271

Following gene: 85374269

Centisome position: 48.9

GC content: 65.17

Gene sequence:

>2211_bases
ATGACGACCGAGCTTGAAAATCTCTCCGAAGCCGAAGCCGCCAATGAATTGATGCGGTTGGCGCGGCAAATCGCGAAGCA
TGATAGGCTCTACCACGCCGAGGATGCGCCGGAGATTACCGACCAGGAATACGATGCGCTGGTGCGGCGCAATGCGGAGC
TGGAAGCGGCGTTTCCGCACCTGGTGCGCGAGGACTCGCCCAGTCGCAAGGTCGGCCATGCGGTCGCGGCCTCGCCGCTG
AGCAAGGTCACCCACGAGGTTCGCATGATGAGCCTCGACAATGCCTTTGCCGACGAGGAGGTGGCCGAGTTTGTCGCGCG
GGTGCGGCGCTATCTCAACATTGGCGAGGACGAGGCGATTGCTTTCACCGCCGAGGACAAGATTGATGGGCTGTCCTGCT
CGCTGCGCTACGAAAATGGCAAACTCGTGCGCGCGGCGACGCGGGGGGACGGGCAGGTAGGCGAGGATGTGACGCCCAAC
GTCGCGCATATCGGCGACATTCCGCAGGAGCTTACTCCCCTCCCGCTTGCGGGAGGGGCTGGGGGTGGGCCACTCGACGA
CAGTGGCTCCGCGCCCACCCCTGACCCCTCCCGCAGGCGGGAGGGGAAATGGAACGGCCCCGCTGTCTTCGAGATCCGAG
GCGAGGTCTATATGGCCACCGCCGACTTCCACGCGCTCAACGCGCGGCTGATGGACGAGGCGCGCGCCGAGGCAGACGAG
AAAGATAACGCGTTCGACACGGCCAAGGTCCGGCAGTTCGCCAATCCGCGCAACGCGGCGGCGGGCTCCTTGCGGCAGAA
AGATGCGAGCGTGACCGCGACCCGTCCGCTCAGGTTCTGGGCGCATGGATGGGGCGCGGTCGAGGGCGATGTGCCGGGCG
AAACGCAGGTCGAAGTCGTCGAGCAGATCGCGGCATGGGGCGTGCCGGTGTCGCCGCTGTTCCGGCGCTGCGAGACGCTC
GAGGAGATGCTCGCGCATTACGAGGCCATCGGCGCGCAGCGGGCGGACTTGCCCTATGAAATCGACGGCGTCGTCTACAA
GGTCGACCGGCTCGATTACCAGCAGCGGCTGGGCTTCGTCGCCAAGGCGCCGCGCTGGGCTATCGCTCGCAAGTTTCCTG
CCGAACAGGCTGAAACGACACTCGAAAATATTGATATTCAGGTCGGGCGGACCGGCAAGCTGACCCCGGTTGGGCGGCTC
GCACCGGTGTTGGTGGGCGGGGTGACGGTGACCAATGTCACGCTCCACAATCGCGACGAAATCGAGCGGCTCGGGGTGCG
CCCCGGCGACCGCGTTGTCGTCCAGCGCGCGGGCGATGTGATTCCGCAAGTCGTGCGCAATCTCACGCCCGACGAGAAAC
GCGATTCTTTCGAGTTTCCCGACACCTGTCCCGAATGCGGCAGCGAAGCGGTGAGCGAGGAAGGGGGGGTCGATGTCCGC
TGCACTGGCGGCCTCATCTGTCCCGCGCAACGCACTGAAAGACTGAAGCATTTCGTCAGCCGCGCCGCGCTCGATATCGA
CGGACTGGGCGAGAAGACGATCGACCAGTTCTTCGCGCTCGGCTGGCTGGAAAGCCCGGCCGATATCTTCCGCTTGAAGG
ACCGGCGCGATGAGATCCTCGCGCTTGAGGGTTGGATGGACAAGTCTGTCGATAACCTGTTGGCATCTGTGGAAGCGCGC
CGCGAACCGGACACCGCACGGCTCCTGTTAGGACTGGGCATCCGCCATGTCGGCGCGGTAACGGCGCGCGACTTGATGAA
GTATTTCCACGAGTTACCGGCGCTTCGTGAAACGGCTGAGAAAGCGCGGGCGGGCGACGAAGAGGCCGTCGTCGCACTCA
CCAGCATCGACGGGATCGGCAGCGCCGTCGTCGAGGCGCTGGGCGATTTTTTCCACGAAGAGCACAATCGCGCAGTGTGG
GACGACCTGCTCAGCGAAGTCAGCCCGCCGCGCTACGAAGTCGAGACGCTCGACAGTCCCGTAGCCGGCAAGACCGTCGT
CTTCACCGGCAAGCTCGAGACCATGAGCCGCGACGAGGCCAAGGCTCAGGCCGAACGGCTCGGCGCCAAGGCCAGCGGCA
GCGTGAGCGCCAAGACCGACTTGCTGGTCGCCGGGCCGGGCGCGGGCAGCAAGCTCAAGAAGGCACAGGACCTCGGCATC
GAAGTGATCGACGAAGCTGGTTGGGCCGAAATCGTCGCAGCGGCGGGATGA

Upstream 100 bases:

>100_bases
CCGTCTTTCGGCGCCACGCCCAAATGCTAATTGAGGCAGACGGTGTTATTCGAGAAGAGGAGGCATACTGGTCTCTGGAA
ATCGATCAGTTTTTCTCTGA

Downstream 100 bases:

>100_bases
GGATGCGCTACACGCTGATCGGGATTGCGTTGGTCCTTGCCGGTTGCGGCAATTCGGGAGGCGAAGATCCCGCCGCGTTC
CAGCAGCGCATCGAGGAAGT

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 736; Mature: 735

Protein sequence:

>736_residues
MTTELENLSEAEAANELMRLARQIAKHDRLYHAEDAPEITDQEYDALVRRNAELEAAFPHLVREDSPSRKVGHAVAASPL
SKVTHEVRMMSLDNAFADEEVAEFVARVRRYLNIGEDEAIAFTAEDKIDGLSCSLRYENGKLVRAATRGDGQVGEDVTPN
VAHIGDIPQELTPLPLAGGAGGGPLDDSGSAPTPDPSRRREGKWNGPAVFEIRGEVYMATADFHALNARLMDEARAEADE
KDNAFDTAKVRQFANPRNAAAGSLRQKDASVTATRPLRFWAHGWGAVEGDVPGETQVEVVEQIAAWGVPVSPLFRRCETL
EEMLAHYEAIGAQRADLPYEIDGVVYKVDRLDYQQRLGFVAKAPRWAIARKFPAEQAETTLENIDIQVGRTGKLTPVGRL
APVLVGGVTVTNVTLHNRDEIERLGVRPGDRVVVQRAGDVIPQVVRNLTPDEKRDSFEFPDTCPECGSEAVSEEGGVDVR
CTGGLICPAQRTERLKHFVSRAALDIDGLGEKTIDQFFALGWLESPADIFRLKDRRDEILALEGWMDKSVDNLLASVEAR
REPDTARLLLGLGIRHVGAVTARDLMKYFHELPALRETAEKARAGDEEAVVALTSIDGIGSAVVEALGDFFHEEHNRAVW
DDLLSEVSPPRYEVETLDSPVAGKTVVFTGKLETMSRDEAKAQAERLGAKASGSVSAKTDLLVAGPGAGSKLKKAQDLGI
EVIDEAGWAEIVAAAG

Sequences:

>Translated_736_residues
MTTELENLSEAEAANELMRLARQIAKHDRLYHAEDAPEITDQEYDALVRRNAELEAAFPHLVREDSPSRKVGHAVAASPL
SKVTHEVRMMSLDNAFADEEVAEFVARVRRYLNIGEDEAIAFTAEDKIDGLSCSLRYENGKLVRAATRGDGQVGEDVTPN
VAHIGDIPQELTPLPLAGGAGGGPLDDSGSAPTPDPSRRREGKWNGPAVFEIRGEVYMATADFHALNARLMDEARAEADE
KDNAFDTAKVRQFANPRNAAAGSLRQKDASVTATRPLRFWAHGWGAVEGDVPGETQVEVVEQIAAWGVPVSPLFRRCETL
EEMLAHYEAIGAQRADLPYEIDGVVYKVDRLDYQQRLGFVAKAPRWAIARKFPAEQAETTLENIDIQVGRTGKLTPVGRL
APVLVGGVTVTNVTLHNRDEIERLGVRPGDRVVVQRAGDVIPQVVRNLTPDEKRDSFEFPDTCPECGSEAVSEEGGVDVR
CTGGLICPAQRTERLKHFVSRAALDIDGLGEKTIDQFFALGWLESPADIFRLKDRRDEILALEGWMDKSVDNLLASVEAR
REPDTARLLLGLGIRHVGAVTARDLMKYFHELPALRETAEKARAGDEEAVVALTSIDGIGSAVVEALGDFFHEEHNRAVW
DDLLSEVSPPRYEVETLDSPVAGKTVVFTGKLETMSRDEAKAQAERLGAKASGSVSAKTDLLVAGPGAGSKLKKAQDLGI
EVIDEAGWAEIVAAAG
>Mature_735_residues
TTELENLSEAEAANELMRLARQIAKHDRLYHAEDAPEITDQEYDALVRRNAELEAAFPHLVREDSPSRKVGHAVAASPLS
KVTHEVRMMSLDNAFADEEVAEFVARVRRYLNIGEDEAIAFTAEDKIDGLSCSLRYENGKLVRAATRGDGQVGEDVTPNV
AHIGDIPQELTPLPLAGGAGGGPLDDSGSAPTPDPSRRREGKWNGPAVFEIRGEVYMATADFHALNARLMDEARAEADEK
DNAFDTAKVRQFANPRNAAAGSLRQKDASVTATRPLRFWAHGWGAVEGDVPGETQVEVVEQIAAWGVPVSPLFRRCETLE
EMLAHYEAIGAQRADLPYEIDGVVYKVDRLDYQQRLGFVAKAPRWAIARKFPAEQAETTLENIDIQVGRTGKLTPVGRLA
PVLVGGVTVTNVTLHNRDEIERLGVRPGDRVVVQRAGDVIPQVVRNLTPDEKRDSFEFPDTCPECGSEAVSEEGGVDVRC
TGGLICPAQRTERLKHFVSRAALDIDGLGEKTIDQFFALGWLESPADIFRLKDRRDEILALEGWMDKSVDNLLASVEARR
EPDTARLLLGLGIRHVGAVTARDLMKYFHELPALRETAEKARAGDEEAVVALTSIDGIGSAVVEALGDFFHEEHNRAVWD
DLLSEVSPPRYEVETLDSPVAGKTVVFTGKLETMSRDEAKAQAERLGAKASGSVSAKTDLLVAGPGAGSKLKKAQDLGIE
VIDEAGWAEIVAAAG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=718, Percent_Identity=46.2395543175487, Blast_Score=568, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ERYLH (Q2N9V6)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458332.1
- ProteinModelPortal:   Q2N9V6
- STRING:   Q2N9V6
- GeneID:   3871832
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07215
- NMPDR:   fig|314225.3.peg.1604
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q2N9V6
- ProtClustDB:   CLSK843976
- BioCyc:   ELIT314225:ELI_07215-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 80158; Mature: 80026

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 127-127 BINDING 125-125 BINDING 148-148 BINDING 215-215 BINDING 347-347 BINDING 371-371

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTELENLSEAEAANELMRLARQIAKHDRLYHAEDAPEITDQEYDALVRRNAELEAAFPH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHH
LVREDSPSRKVGHAVAASPLSKVTHEVRMMSLDNAFADEEVAEFVARVRRYLNIGEDEAI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEE
AFTAEDKIDGLSCSLRYENGKLVRAATRGDGQVGEDVTPNVAHIGDIPQELTPLPLAGGA
EEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCCC
GGGPLDDSGSAPTPDPSRRREGKWNGPAVFEIRGEVYMATADFHALNARLMDEARAEADE
CCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCC
KDNAFDTAKVRQFANPRNAAAGSLRQKDASVTATRPLRFWAHGWGAVEGDVPGETQVEVV
CCCCHHHHHHHHHCCCCCHHHCCHHHCCCCCEECCCHHHHHCCCCCCCCCCCCCHHHHHH
EQIAAWGVPVSPLFRRCETLEEMLAHYEAIGAQRADLPYEIDGVVYKVDRLDYQQRLGFV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEHHHCHHHHCCHH
AKAPRWAIARKFPAEQAETTLENIDIQVGRTGKLTPVGRLAPVLVGGVTVTNVTLHNRDE
HCCCCHHHHHHCCHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHCCCEEEEEEEECCHHH
IERLGVRPGDRVVVQRAGDVIPQVVRNLTPDEKRDSFEFPDTCPECGSEAVSEEGGVDVR
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEE
CTGGLICPAQRTERLKHFVSRAALDIDGLGEKTIDQFFALGWLESPADIFRLKDRRDEIL
ECCCEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEE
ALEGWMDKSVDNLLASVEARREPDTARLLLGLGIRHVGAVTARDLMKYFHELPALRETAE
EECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
KARAGDEEAVVALTSIDGIGSAVVEALGDFFHEEHNRAVWDDLLSEVSPPRYEVETLDSP
HHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEHHHCCCC
VAGKTVVFTGKLETMSRDEAKAQAERLGAKASGSVSAKTDLLVAGPGAGSKLKKAQDLGI
CCCCEEEEECCHHHHCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCC
EVIDEAGWAEIVAAAG
EEECCCCHHHHHCCCC
>Mature Secondary Structure 
TTELENLSEAEAANELMRLARQIAKHDRLYHAEDAPEITDQEYDALVRRNAELEAAFPH
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHH
LVREDSPSRKVGHAVAASPLSKVTHEVRMMSLDNAFADEEVAEFVARVRRYLNIGEDEAI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEE
AFTAEDKIDGLSCSLRYENGKLVRAATRGDGQVGEDVTPNVAHIGDIPQELTPLPLAGGA
EEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCCC
GGGPLDDSGSAPTPDPSRRREGKWNGPAVFEIRGEVYMATADFHALNARLMDEARAEADE
CCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCC
KDNAFDTAKVRQFANPRNAAAGSLRQKDASVTATRPLRFWAHGWGAVEGDVPGETQVEVV
CCCCHHHHHHHHHCCCCCHHHCCHHHCCCCCEECCCHHHHHCCCCCCCCCCCCCHHHHHH
EQIAAWGVPVSPLFRRCETLEEMLAHYEAIGAQRADLPYEIDGVVYKVDRLDYQQRLGFV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEHHHCHHHHCCHH
AKAPRWAIARKFPAEQAETTLENIDIQVGRTGKLTPVGRLAPVLVGGVTVTNVTLHNRDE
HCCCCHHHHHHCCHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHCCCEEEEEEEECCHHH
IERLGVRPGDRVVVQRAGDVIPQVVRNLTPDEKRDSFEFPDTCPECGSEAVSEEGGVDVR
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEE
CTGGLICPAQRTERLKHFVSRAALDIDGLGEKTIDQFFALGWLESPADIFRLKDRRDEIL
ECCCEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEE
ALEGWMDKSVDNLLASVEARREPDTARLLLGLGIRHVGAVTARDLMKYFHELPALRETAE
EECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
KARAGDEEAVVALTSIDGIGSAVVEALGDFFHEEHNRAVWDDLLSEVSPPRYEVETLDSP
HHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEHHHCCCC
VAGKTVVFTGKLETMSRDEAKAQAERLGAKASGSVSAKTDLLVAGPGAGSKLKKAQDLGI
CCCCEEEEECCHHHHCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCC
EVIDEAGWAEIVAAAG
EEECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA