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The map label for this gene is phrB [H]

Identifier: 85374236

GI number: 85374236

Start: 1460157

End: 1461548

Strand: Reverse

Name: phrB [H]

Synonym: ELI_07045

Alternate gene names: 85374236

Gene position: 1461548-1460157 (Counterclockwise)

Preceding gene: 85374237

Following gene: 85374234

Centisome position: 47.88

GC content: 62.86

Gene sequence:

>1392_bases
ATGACCAAACCCCAAATCGTATGGCTGCGGCGCGATCTGCGCATGGCGGACAATCCGGCGCTGTATCATGCGGCCAAGGC
CGGACCGGTGGTGGCGGTCTATGTGCTCGACGACGAACGGCCCAAGGATCACAAGCTGGGCGGGGCGGCGCGGTGGTGGC
TGCATCATTCGCTGGAGAGCCTGGGCAAGAGCTTCGGCGCGCGCAATTCGCGGATTGTCCTGCGCCGCGGCGATGCGGTG
GAGGAGCTGAAAACCGTCGCCAATGCTGTCGGCGCAGAGACCGTCCATGCCAATCGTCACTATGAGCCGTGGTGGCGCAA
GGCGCAGGGCGAGCTGGCCGAAGAACTGGAGCTCAAGCTCTATGACGGCAACTACCTATTTCCGCCGGGCCACGTGACGA
CAGGATCGGGCGATCCCTACAAGATCTACACGCCGTTCTCCAAGGCGATGCTGCAGGAAATGCCCCCGCGCGATGAAGTG
CCGGAGCCGGAGACGCTGTCCTCACCGGGTGAGTGGCCCGCCAGCGACGATCTGGGCGACTGGAACCTGCTGCCGACCAA
GCCCGACTGGGCCGGCGGCCTGCGCGACTTCTGGACCGTCGGCGAAGACGCGGCGCATGAGCGGCTCGACTGGTGGTCCG
ACGAGGTCGACAAATACGATGACGGGCGCAACATGCCCTCGGTCGACGAAACCAGCCAGATGTCGCCGCACCTCCATCAT
GGCGAGATCTCGCCGGTCCAGATCTGGCACCGCTTCAAGGACAAGCGCTCCGACGGGTGGAAGACTTACGAGAAAGAGCT
GATCTGGCGCGACTATGCGCAAAACGTGATCTGCCAGTTCCCGGCCTATCCGAAAGAGAGCTATCGCGACGGCTGGGATG
CCGACATGGCGTGGCGCAATCCCAATCGCGGGCACATCATTCAGGAGGAGCTGAAACGCTGGCAGAAAGGGCAGACCGGC
TATCCGATCGTCGATGCCGGTATGCGGCAGCTATGGCAGACCGGCTGGATGCATAACCGCGTGCGGATGATCGCCGCGAG
CTTCCTCATCAAGCACCTGCTGATCGACTGGCGGCACGGCGAAAAATGGTTTTGGGATACGCTGGTGGATGCCGACTATG
CCAGCAACGGCACCAACTGGCAGTGGGTCAGCGGCACCGGCGTCGACAGCAACATGTTCAGCCGGATCATGGCGCCGCTG
ACGCAGAGCGAGAAGTTCGATGCGGCAGGCTATATCCGGGAATACGTGCCCGAACTGGCGGACTTGCCCGACGAGACGAT
CCACGATCCCGAAGAGCACGGCTGCAAGCCGGACGATTACCCCTCGAAGATCATCGCCCACAAGGAAGGCCGCGAGCGCG
CGCTCGAAGCCTATAGCGAATTCAAGAACTGA

Upstream 100 bases:

>100_bases
CGTCCAGGTATATCCCGCCTACTCTGGGGGAGGACCGACTGTTGTGGCTGTACCGGAACCCCCTGACGACTAACCACGTT
GGGCATCCGAAGGGATAAGG

Downstream 100 bases:

>100_bases
GGCGCGATTTCAGCAACTGGTCGGCCAGCAGCTTGCCCATCACCGGCACGAAGATCGCCGCCACGACCATCCAGGTCGGG
TGCGGAAACATCTGCGTCGT

Product: hypothetical protein

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTKPQIVWLRRDLRMADNPALYHAAKAGPVVAVYVLDDERPKDHKLGGAARWWLHHSLESLGKSFGARNSRIVLRRGDAV
EELKTVANAVGAETVHANRHYEPWWRKAQGELAEELELKLYDGNYLFPPGHVTTGSGDPYKIYTPFSKAMLQEMPPRDEV
PEPETLSSPGEWPASDDLGDWNLLPTKPDWAGGLRDFWTVGEDAAHERLDWWSDEVDKYDDGRNMPSVDETSQMSPHLHH
GEISPVQIWHRFKDKRSDGWKTYEKELIWRDYAQNVICQFPAYPKESYRDGWDADMAWRNPNRGHIIQEELKRWQKGQTG
YPIVDAGMRQLWQTGWMHNRVRMIAASFLIKHLLIDWRHGEKWFWDTLVDADYASNGTNWQWVSGTGVDSNMFSRIMAPL
TQSEKFDAAGYIREYVPELADLPDETIHDPEEHGCKPDDYPSKIIAHKEGRERALEAYSEFKN

Sequences:

>Translated_463_residues
MTKPQIVWLRRDLRMADNPALYHAAKAGPVVAVYVLDDERPKDHKLGGAARWWLHHSLESLGKSFGARNSRIVLRRGDAV
EELKTVANAVGAETVHANRHYEPWWRKAQGELAEELELKLYDGNYLFPPGHVTTGSGDPYKIYTPFSKAMLQEMPPRDEV
PEPETLSSPGEWPASDDLGDWNLLPTKPDWAGGLRDFWTVGEDAAHERLDWWSDEVDKYDDGRNMPSVDETSQMSPHLHH
GEISPVQIWHRFKDKRSDGWKTYEKELIWRDYAQNVICQFPAYPKESYRDGWDADMAWRNPNRGHIIQEELKRWQKGQTG
YPIVDAGMRQLWQTGWMHNRVRMIAASFLIKHLLIDWRHGEKWFWDTLVDADYASNGTNWQWVSGTGVDSNMFSRIMAPL
TQSEKFDAAGYIREYVPELADLPDETIHDPEEHGCKPDDYPSKIIAHKEGRERALEAYSEFKN
>Mature_462_residues
TKPQIVWLRRDLRMADNPALYHAAKAGPVVAVYVLDDERPKDHKLGGAARWWLHHSLESLGKSFGARNSRIVLRRGDAVE
ELKTVANAVGAETVHANRHYEPWWRKAQGELAEELELKLYDGNYLFPPGHVTTGSGDPYKIYTPFSKAMLQEMPPRDEVP
EPETLSSPGEWPASDDLGDWNLLPTKPDWAGGLRDFWTVGEDAAHERLDWWSDEVDKYDDGRNMPSVDETSQMSPHLHHG
EISPVQIWHRFKDKRSDGWKTYEKELIWRDYAQNVICQFPAYPKESYRDGWDADMAWRNPNRGHIIQEELKRWQKGQTGY
PIVDAGMRQLWQTGWMHNRVRMIAASFLIKHLLIDWRHGEKWFWDTLVDADYASNGTNWQWVSGTGVDSNMFSRIMAPLT
QSEKFDAAGYIREYVPELADLPDETIHDPEEHGCKPDDYPSKIIAHKEGRERALEAYSEFKN

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=501, Percent_Identity=26.5469061876248, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4758072, Length=494, Percent_Identity=26.1133603238866, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI188536103, Length=460, Percent_Identity=25.4347826086956, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786926, Length=476, Percent_Identity=34.453781512605, Blast_Score=251, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6324962, Length=512, Percent_Identity=29.296875, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17137248, Length=507, Percent_Identity=28.0078895463511, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24585455, Length=507, Percent_Identity=28.0078895463511, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24648152, Length=524, Percent_Identity=24.618320610687, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53590; Mature: 53459

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPQIVWLRRDLRMADNPALYHAAKAGPVVAVYVLDDERPKDHKLGGAARWWLHHSLES
CCCCCEEEEEHHHCCCCCCHHEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LGKSFGARNSRIVLRRGDAVEELKTVANAVGAETVHANRHYEPWWRKAQGELAEELELKL
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHEEEE
YDGNYLFPPGHVTTGSGDPYKIYTPFSKAMLQEMPPRDEVPEPETLSSPGEWPASDDLGD
ECCCEECCCCCEECCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
WNLLPTKPDWAGGLRDFWTVGEDAAHERLDWWSDEVDKYDDGRNMPSVDETSQMSPHLHH
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
GEISPVQIWHRFKDKRSDGWKTYEKELIWRDYAQNVICQFPAYPKESYRDGWDADMAWRN
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCC
PNRGHIIQEELKRWQKGQTGYPIVDAGMRQLWQTGWMHNRVRMIAASFLIKHLLIDWRHG
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
EKWFWDTLVDADYASNGTNWQWVSGTGVDSNMFSRIMAPLTQSEKFDAAGYIREYVPELA
CCHHHHHHHCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DLPDETIHDPEEHGCKPDDYPSKIIAHKEGRERALEAYSEFKN
CCCCHHCCCCHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKPQIVWLRRDLRMADNPALYHAAKAGPVVAVYVLDDERPKDHKLGGAARWWLHHSLES
CCCCEEEEEHHHCCCCCCHHEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LGKSFGARNSRIVLRRGDAVEELKTVANAVGAETVHANRHYEPWWRKAQGELAEELELKL
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHEEEE
YDGNYLFPPGHVTTGSGDPYKIYTPFSKAMLQEMPPRDEVPEPETLSSPGEWPASDDLGD
ECCCEECCCCCEECCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
WNLLPTKPDWAGGLRDFWTVGEDAAHERLDWWSDEVDKYDDGRNMPSVDETSQMSPHLHH
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
GEISPVQIWHRFKDKRSDGWKTYEKELIWRDYAQNVICQFPAYPKESYRDGWDADMAWRN
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCC
PNRGHIIQEELKRWQKGQTGYPIVDAGMRQLWQTGWMHNRVRMIAASFLIKHLLIDWRHG
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
EKWFWDTLVDADYASNGTNWQWVSGTGVDSNMFSRIMAPLTQSEKFDAAGYIREYVPELA
CCHHHHHHHCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DLPDETIHDPEEHGCKPDDYPSKIIAHKEGRERALEAYSEFKN
CCCCHHCCCCHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]