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The map label for this gene is asnB [H]

Identifier: 85374190

GI number: 85374190

Start: 1411105

End: 1413003

Strand: Reverse

Name: asnB [H]

Synonym: ELI_06815

Alternate gene names: 85374190

Gene position: 1413003-1411105 (Counterclockwise)

Preceding gene: 85374191

Following gene: 85374189

Centisome position: 46.29

GC content: 64.67

Gene sequence:

>1899_bases
ATGTGCGGCATTGCCGGGATCTTTCACTGCGGAACGCCCAAGCCGGTCGATCCCCGCCGGATCGAACGTATGACCGATGC
GATGGTGCATCGCGGCCCCGACGGCGGTGGCGTCTGGACCGATCAGGGCGTGGGGCTCGGCCACAGGCGGCTGTCGATCA
TCGACATCGAAGGGTCGCCGCAGCCCATGCTATCCGCCGATGAGCGCGCGATGCTGGTCTTCAACGGCGAAATCTACAAC
TATCGCGATTTGCGCCGTGAGCTGAAGGAACTCGGCGCTCGCTTCCACACCGACGGCGATTCGGAGGTCATCCTCGCCGC
ATGGCAGAAATGGGGCCCCGCATGCCTGTCGCGGCTCGACGGCATGTTCGCCTTCGCCATCTACGACCGCACCGAGCGGG
TGCTGTTCCTCGCCCGCGACCGCTTTGGCGTGAAGCCGCTATACATGGCGAACCTGAGCGACGGCAGCCTTGCTTTCGCG
TCAGAACTGAAGGGCCTGCTGGCGCATCCGCTGCTACGGCGCCAGGCCGATCCACTGGCGGTCGAGGACTACCTGACCTG
GGGCTATGTGCCGGATCATCGCTGCATCCTCAAAGGCGTCGAGAAACTGCCCGCCGGGCACTACCGCCTGTTGCGACATG
GCGGACCGCCTGCGCCATCCGTGCAATGGTGGGACATCGATTTCTCCGGCAGGCACAGTGGCAGCACTGCCGACCTGTCG
GCGCAATTGCTGCATCTGATGCGCGAGGGGGTGGAATCGCGGATGACGGCCGATGTGCCGCTGGGCGCGTTTCTTTCCGG
CGGGGTCGACAGCTCCAGCGTCGTCGCGCTGATGGCCGATGCCAGCCGTGAGCCGGTCAACACATGTTCGATCGGCTTCG
ATGTCGCAGCGCTGGACGAGACCGGTTACGCGCGCCGGATTGCCGAACGATTCGAGACGGATCACCGCGAGCGGATCGTC
GATCCCGACGATTTCGGGGCGGTCGACCTGCTGGCGGGCATGTTCGACGAACCATTCGCCGATGCCTCCGCCTTGCCGAC
CTGGCGCGTCTGCCAACTTGCGCGCGAGCACGTGACCGTCGCGCTGTCGGGCGATGGTGCCGACGAGGCGTTTGCGGGCT
ATCGCCGGCAGGTCTTCCATGCGCGCGAGGAGCAGGTTCGCTCTATCCTGCCACAGGCGCTGCGCGGGCCGATCCTCGGT
GGGCTCGGACGCATCTGGCCCAAGGCCGATTGGGCTCCGCGGCCCTTGCGCGCGAAATCCACGCTGCAAGCGCTCGGTGC
GACTGGCGAGGAAGGCTATGCGCGCGCCCTGGCTGTGACGGCGCCGGAAATGCGCTCCCAGCTCTATACCGATGCGATGG
AGCGGCAGCTTGACGGATATCGCGCGGAGCAACCCTTCGTCGAGCTGATGCGAAATGCGCCGGCCCGCTCCGGCCTCGAC
CGGGCACAATATGCCGACCTCAAGTTCTGGCTGCCCGGCGACATCCTGACCAAGGTCGATCGCACCAGCATGGCTGTCAG
CCTCGAAGCGCGCGAGCCTCTGCTCGACCACCGCCTGGTCGAATTTGCGGCCGGATTGCCTTACGGAGCGCGCATCAAGG
GCCGCCAGGGCAAGTGGCTTTTAAAGCACACTATGGAGCGCTTTCTTCCCCGGGATGTGCTGTATCGGCCCAAGCAGGGC
TTCGTGACACCGATCGCCGAATGGCTGCGCGGACCGCTACAGAACGAAGCCCGCGCCATCGCCACCAGTGCCACCATTGC
CGACACCGGCTGGTTCTCAACTTCGCGGCTTAAACGCCTGACAGAGAATCATATTTCCGGTTCTGCCGATCATTCCCGAT
TGCTGTGGCAATTGCTGATGCTCGACCGTTCCATCGGAAATCTGGCGATAACTCTGTGA

Upstream 100 bases:

>100_bases
CGATCAGACACCGGACGCGGCGCTGGCCGCATTCCGCCAGTCCACAGGGCCGCTCGGACCGTGGATAGACCGCATTGTGG
AAAGCCGATAACTCGCGTCC

Downstream 100 bases:

>100_bases
CTTAAATGCCCCAGTTCGGGACTCGGTCCATCCGCCCTCGACAGTTTCCCGATTGCCAAAGGCGCGGCCCGTCGCGTAGC
TTACGTTTGTTGGGAGCAAA

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MCGIAGIFHCGTPKPVDPRRIERMTDAMVHRGPDGGGVWTDQGVGLGHRRLSIIDIEGSPQPMLSADERAMLVFNGEIYN
YRDLRRELKELGARFHTDGDSEVILAAWQKWGPACLSRLDGMFAFAIYDRTERVLFLARDRFGVKPLYMANLSDGSLAFA
SELKGLLAHPLLRRQADPLAVEDYLTWGYVPDHRCILKGVEKLPAGHYRLLRHGGPPAPSVQWWDIDFSGRHSGSTADLS
AQLLHLMREGVESRMTADVPLGAFLSGGVDSSSVVALMADASREPVNTCSIGFDVAALDETGYARRIAERFETDHRERIV
DPDDFGAVDLLAGMFDEPFADASALPTWRVCQLAREHVTVALSGDGADEAFAGYRRQVFHAREEQVRSILPQALRGPILG
GLGRIWPKADWAPRPLRAKSTLQALGATGEEGYARALAVTAPEMRSQLYTDAMERQLDGYRAEQPFVELMRNAPARSGLD
RAQYADLKFWLPGDILTKVDRTSMAVSLEAREPLLDHRLVEFAAGLPYGARIKGRQGKWLLKHTMERFLPRDVLYRPKQG
FVTPIAEWLRGPLQNEARAIATSATIADTGWFSTSRLKRLTENHISGSADHSRLLWQLLMLDRSIGNLAITL

Sequences:

>Translated_632_residues
MCGIAGIFHCGTPKPVDPRRIERMTDAMVHRGPDGGGVWTDQGVGLGHRRLSIIDIEGSPQPMLSADERAMLVFNGEIYN
YRDLRRELKELGARFHTDGDSEVILAAWQKWGPACLSRLDGMFAFAIYDRTERVLFLARDRFGVKPLYMANLSDGSLAFA
SELKGLLAHPLLRRQADPLAVEDYLTWGYVPDHRCILKGVEKLPAGHYRLLRHGGPPAPSVQWWDIDFSGRHSGSTADLS
AQLLHLMREGVESRMTADVPLGAFLSGGVDSSSVVALMADASREPVNTCSIGFDVAALDETGYARRIAERFETDHRERIV
DPDDFGAVDLLAGMFDEPFADASALPTWRVCQLAREHVTVALSGDGADEAFAGYRRQVFHAREEQVRSILPQALRGPILG
GLGRIWPKADWAPRPLRAKSTLQALGATGEEGYARALAVTAPEMRSQLYTDAMERQLDGYRAEQPFVELMRNAPARSGLD
RAQYADLKFWLPGDILTKVDRTSMAVSLEAREPLLDHRLVEFAAGLPYGARIKGRQGKWLLKHTMERFLPRDVLYRPKQG
FVTPIAEWLRGPLQNEARAIATSATIADTGWFSTSRLKRLTENHISGSADHSRLLWQLLMLDRSIGNLAITL
>Mature_632_residues
MCGIAGIFHCGTPKPVDPRRIERMTDAMVHRGPDGGGVWTDQGVGLGHRRLSIIDIEGSPQPMLSADERAMLVFNGEIYN
YRDLRRELKELGARFHTDGDSEVILAAWQKWGPACLSRLDGMFAFAIYDRTERVLFLARDRFGVKPLYMANLSDGSLAFA
SELKGLLAHPLLRRQADPLAVEDYLTWGYVPDHRCILKGVEKLPAGHYRLLRHGGPPAPSVQWWDIDFSGRHSGSTADLS
AQLLHLMREGVESRMTADVPLGAFLSGGVDSSSVVALMADASREPVNTCSIGFDVAALDETGYARRIAERFETDHRERIV
DPDDFGAVDLLAGMFDEPFADASALPTWRVCQLAREHVTVALSGDGADEAFAGYRRQVFHAREEQVRSILPQALRGPILG
GLGRIWPKADWAPRPLRAKSTLQALGATGEEGYARALAVTAPEMRSQLYTDAMERQLDGYRAEQPFVELMRNAPARSGLD
RAQYADLKFWLPGDILTKVDRTSMAVSLEAREPLLDHRLVEFAAGLPYGARIKGRQGKWLLKHTMERFLPRDVLYRPKQG
FVTPIAEWLRGPLQNEARAIATSATIADTGWFSTSRLKRLTENHISGSADHSRLLWQLLMLDRSIGNLAITL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=392, Percent_Identity=28.0612244897959, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI168229252, Length=392, Percent_Identity=28.0612244897959, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI168229250, Length=392, Percent_Identity=28.0612244897959, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI296010848, Length=365, Percent_Identity=27.9452054794521, Blast_Score=116, Evalue=7e-26,
Organism=Escherichia coli, GI1786889, Length=412, Percent_Identity=29.3689320388349, Blast_Score=152, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71993933, Length=392, Percent_Identity=31.3775510204082, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25147557, Length=392, Percent_Identity=31.3775510204082, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25147560, Length=330, Percent_Identity=32.4242424242424, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=416, Percent_Identity=27.4038461538462, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321563, Length=415, Percent_Identity=26.7469879518072, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45553209, Length=398, Percent_Identity=31.9095477386935, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70213; Mature: 70213

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIFHCGTPKPVDPRRIERMTDAMVHRGPDGGGVWTDQGVGLGHRRLSIIDIEGSP
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEEEEECCCC
QPMLSADERAMLVFNGEIYNYRDLRRELKELGARFHTDGDSEVILAAWQKWGPACLSRLD
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHC
GMFAFAIYDRTERVLFLARDRFGVKPLYMANLSDGSLAFASELKGLLAHPLLRRQADPLA
CCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
VEDYLTWGYVPDHRCILKGVEKLPAGHYRLLRHGGPPAPSVQWWDIDFSGRHSGSTADLS
HHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHH
AQLLHLMREGVESRMTADVPLGAFLSGGVDSSSVVALMADASREPVNTCSIGFDVAALDE
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCEEECCCEEEECCC
TGYARRIAERFETDHRERIVDPDDFGAVDLLAGMFDEPFADASALPTWRVCQLAREHVTV
CHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEE
ALSGDGADEAFAGYRRQVFHAREEQVRSILPQALRGPILGGLGRIWPKADWAPRPLRAKS
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHH
TLQALGATGEEGYARALAVTAPEMRSQLYTDAMERQLDGYRAEQPFVELMRNAPARSGLD
HHHHHCCCCCCCHHHEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
RAQYADLKFWLPGDILTKVDRTSMAVSLEAREPLLDHRLVEFAAGLPYGARIKGRQGKWL
HHHHCCEEEECCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEECCCCCCHH
LKHTMERFLPRDVLYRPKQGFVTPIAEWLRGPLQNEARAIATSATIADTGWFSTSRLKRL
HHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCEECCCCCCCHHHHHHH
TENHISGSADHSRLLWQLLMLDRSIGNLAITL
HHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MCGIAGIFHCGTPKPVDPRRIERMTDAMVHRGPDGGGVWTDQGVGLGHRRLSIIDIEGSP
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEEEEECCCC
QPMLSADERAMLVFNGEIYNYRDLRRELKELGARFHTDGDSEVILAAWQKWGPACLSRLD
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHC
GMFAFAIYDRTERVLFLARDRFGVKPLYMANLSDGSLAFASELKGLLAHPLLRRQADPLA
CCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
VEDYLTWGYVPDHRCILKGVEKLPAGHYRLLRHGGPPAPSVQWWDIDFSGRHSGSTADLS
HHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHH
AQLLHLMREGVESRMTADVPLGAFLSGGVDSSSVVALMADASREPVNTCSIGFDVAALDE
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCEEECCCEEEECCC
TGYARRIAERFETDHRERIVDPDDFGAVDLLAGMFDEPFADASALPTWRVCQLAREHVTV
CHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEE
ALSGDGADEAFAGYRRQVFHAREEQVRSILPQALRGPILGGLGRIWPKADWAPRPLRAKS
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHH
TLQALGATGEEGYARALAVTAPEMRSQLYTDAMERQLDGYRAEQPFVELMRNAPARSGLD
HHHHHCCCCCCCHHHEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
RAQYADLKFWLPGDILTKVDRTSMAVSLEAREPLLDHRLVEFAAGLPYGARIKGRQGKWL
HHHHCCEEEECCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEECCCCCCHH
LKHTMERFLPRDVLYRPKQGFVTPIAEWLRGPLQNEARAIATSATIADTGWFSTSRLKRL
HHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCEECCCCCCCHHHHHHH
TENHISGSADHSRLLWQLLMLDRSIGNLAITL
HHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]