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The map label for this gene is fadB [H]

Identifier: 85374189

GI number: 85374189

Start: 1410006

End: 1410875

Strand: Reverse

Name: fadB [H]

Synonym: ELI_06810

Alternate gene names: 85374189

Gene position: 1410875-1410006 (Counterclockwise)

Preceding gene: 85374190

Following gene: 85374185

Centisome position: 46.22

GC content: 63.22

Gene sequence:

>870_bases
ATGAGGAGCCTTGCGAAGGGACACGCCGGTTTGCCGGACGAATTGTTCGCACTGAGGGAACTCGATGTTCTCTACGATCC
CGACCGGCAAACTCTGTGGACCTATATGCGTCCGCAGGGGCGCCCCAGCTTTACCCCGCCGATGCTCGGCGATTTCGAGA
ATTGGCAACGGCTGATCGCCGCCAATTTCGGGCCGGACAAGGTCCCGTTGCAGTTCCTCGTGCTCGCAAGCCGCGCGCCC
GGCGTGTTCTGTTATGGCGGCGATCTGGCGCTGTTCCGGCAGTTGATCGTCGATCGCAACCGCGATGGGCTGGTTCGTTA
CGGACATCGGTGCTGCGAGATCCTGCACCGCAATATCAACACGCTCAATGTGCCGATGCTGACGGTCGGCATGGTCGAGG
GTGCCGCACTGGGGGGCGGGTTCGAGGCCCTGCTGTCATTCGATTACATTGTGGCGGAACGCAGCGCGACTTTCGGTCTT
CCCGAAATCATGTTCGGGCTGTTCCCCGGCATGGGCGCGCACGCTTTGCTCAGCCGCAAGATCGGCAGCGCGATGGCGGA
CCGGCTCATCGTGTCCAACAAGACCTACACGGCCGAAGAGATGTACGAGCTCGGCCTGGTGCACCACTTGGCAGAGGATG
GCGGGGCGCTGGAGGCGACGCGCGATTTCCTCAAGAAGTCCGAGCGGCGCCATGCCGGTCTGGTGGCAGCACGACGTGCG
ATGAAGGTCTCGTGGAACCTCAGCCTGACCGAGCTGACCCGGATCACCGAGCTTTGGGCCGATGCGGCGCTGCAATTGCG
TGAGCAGGATCTCAAACTGATGGACCGGCTCGCCTCCGCGCAGGAACGGGGCGCGCGCAAGGCCGCCTAA

Upstream 100 bases:

>100_bases
GGGGAGTCGCATGGACCGAGTGGGAACTGAATTCGAGGACGGCATCATAGCCGACAGCGGGGGATCGCAGTCCCCGGTCG
GCGAAATAGGAGGCTCAGCT

Downstream 100 bases:

>100_bases
GCTCTCGCCACTTCGTCTTCGCCATGGCCGAGCGCCATGTATTCGTCGCTGCGCATCTCCATCAGGCGGCTGACGGTGCG
TTCGAACTCGAAGCTCCCGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRSLAKGHAGLPDELFALRELDVLYDPDRQTLWTYMRPQGRPSFTPPMLGDFENWQRLIAANFGPDKVPLQFLVLASRAP
GVFCYGGDLALFRQLIVDRNRDGLVRYGHRCCEILHRNINTLNVPMLTVGMVEGAALGGGFEALLSFDYIVAERSATFGL
PEIMFGLFPGMGAHALLSRKIGSAMADRLIVSNKTYTAEEMYELGLVHHLAEDGGALEATRDFLKKSERRHAGLVAARRA
MKVSWNLSLTELTRITELWADAALQLREQDLKLMDRLASAQERGARKAA

Sequences:

>Translated_289_residues
MRSLAKGHAGLPDELFALRELDVLYDPDRQTLWTYMRPQGRPSFTPPMLGDFENWQRLIAANFGPDKVPLQFLVLASRAP
GVFCYGGDLALFRQLIVDRNRDGLVRYGHRCCEILHRNINTLNVPMLTVGMVEGAALGGGFEALLSFDYIVAERSATFGL
PEIMFGLFPGMGAHALLSRKIGSAMADRLIVSNKTYTAEEMYELGLVHHLAEDGGALEATRDFLKKSERRHAGLVAARRA
MKVSWNLSLTELTRITELWADAALQLREQDLKLMDRLASAQERGARKAA
>Mature_289_residues
MRSLAKGHAGLPDELFALRELDVLYDPDRQTLWTYMRPQGRPSFTPPMLGDFENWQRLIAANFGPDKVPLQFLVLASRAP
GVFCYGGDLALFRQLIVDRNRDGLVRYGHRCCEILHRNINTLNVPMLTVGMVEGAALGGGFEALLSFDYIVAERSATFGL
PEIMFGLFPGMGAHALLSRKIGSAMADRLIVSNKTYTAEEMYELGLVHHLAEDGGALEATRDFLKKSERRHAGLVAARRA
MKVSWNLSLTELTRITELWADAALQLREQDLKLMDRLASAQERGARKAA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the 3- hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787659, Length=132, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788682, Length=178, Percent_Identity=29.2134831460674, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790281, Length=171, Percent_Identity=27.4853801169591, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=27.3170731707317, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24653139, Length=178, Percent_Identity=30.3370786516854, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR018376
- InterPro:   IPR012799
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =5.3.3.8; =1.1.1.35 [H]

Molecular weight: Translated: 32204; Mature: 32204

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLAKGHAGLPDELFALRELDVLYDPDRQTLWTYMRPQGRPSFTPPMLGDFENWQRLIA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
ANFGPDKVPLQFLVLASRAPGVFCYGGDLALFRQLIVDRNRDGLVRYGHRCCEILHRNIN
CCCCCCCCCHHHHEECCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
TLNVPMLTVGMVEGAALGGGFEALLSFDYIVAERSATFGLPEIMFGLFPGMGAHALLSRK
EECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHH
IGSAMADRLIVSNKTYTAEEMYELGLVHHLAEDGGALEATRDFLKKSERRHAGLVAARRA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MKVSWNLSLTELTRITELWADAALQLREQDLKLMDRLASAQERGARKAA
HHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRSLAKGHAGLPDELFALRELDVLYDPDRQTLWTYMRPQGRPSFTPPMLGDFENWQRLIA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
ANFGPDKVPLQFLVLASRAPGVFCYGGDLALFRQLIVDRNRDGLVRYGHRCCEILHRNIN
CCCCCCCCCHHHHEECCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
TLNVPMLTVGMVEGAALGGGFEALLSFDYIVAERSATFGLPEIMFGLFPGMGAHALLSRK
EECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHH
IGSAMADRLIVSNKTYTAEEMYELGLVHHLAEDGGALEATRDFLKKSERRHAGLVAARRA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MKVSWNLSLTELTRITELWADAALQLREQDLKLMDRLASAQERGARKAA
HHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA